19
14 14 C 13 13 C 2 H 18 18 O Mariusz Fogtman and Anna Pazdur Mariusz Fogtman and Anna Pazdur Silesian University of Silesian University of Technology Technology Department of Radioisotopes Department of Radioisotopes www.carbon14. pl www.carbon14.pl

14 C 13 C 2H2H2H2H 18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

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Page 1: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

1414CC

1313CC22HH

1818OO

Mariusz Fogtman and Anna PazdurMariusz Fogtman and Anna Pazdur

Silesian University of TechnologySilesian University of TechnologyDepartment of RadioisotopesDepartment of Radioisotopes

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Page 2: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

Scientists can use the tree rings for reconstructing past climate because trees are good environmental indicators. They produce very small rings during years of drought and large rings during years of good growing conditions. 

By counting the rings from the middle of the trunk, and studying the width of the rings, scientists can reconstruct an approximate calendar of wet and dry years. Scientists assume that the weather affected ancient tree ring growth the same way it does today. Scientists can also date the occurrence and frequency of fires by finding scars that appear in the growth rings.

Additionally measurement of isotopic ratios e.g. 2H, 13C, 18O and others in annual tree rings by mass spectrometer allows to track the isotopic ratios changes to determine the climate conditions in the past.

INTRODUCTIONINTRODUCTION

Page 3: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

CHARACTERISTIC OF TREE COMPONENTSCHARACTERISTIC OF TREE COMPONENTS

Trees go through annual Trees go through annual cycles of growth. Roots cycles of growth. Roots

are busy in the early are busy in the early spring and late fall. spring and late fall.

Leaves and twigs grow in Leaves and twigs grow in spring and the tree adds spring and the tree adds wood all summer long.wood all summer long.

http://www.na.fs.fed.us/spfo/pubs/uf/treeguidehtm/chapter4.htmhttp://www.na.fs.fed.us/spfo/pubs/uf/treeguidehtm/chapter4.htm

Page 4: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

INSIDE THE TREEINSIDE THE TREE

CrossCross--section of section of the the tree trunktree trunk..

Page 5: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

INSIDE THE TREEINSIDE THE TREE

Tree ring widthTree ring width Variability of tree ring width and climatic conditionsVariability of tree ring width and climatic conditions Seasonal patterns:Seasonal patterns:           Early wood Large, thick-walled cells           Early wood Large, thick-walled cells           Late wood Small, densely-packed, thin-walled          Late wood Small, densely-packed, thin-walled cells cells           Together = an annual growth ring           Together = an annual growth ring Mean width of rings dependant on:Mean width of rings dependant on:           tree species           tree species           tree age           tree age           availability of stored food           availability of stored food           climate (precipitation, temperature, humidity,           climate (precipitation, temperature, humidity, sunshine, sunshine,           windspeed, humidity)           windspeed, humidity) Trees as filters and sources of palaeoclimatic dataTrees as filters and sources of palaeoclimatic data

Page 6: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

TREE SAMPLINGTREE SAMPLING

TREE SLICE SAMPLINGTREE SLICE SAMPLING

Increment borerIncrement borer

Collection of the tree slice

Tree slice surface preparation by polishing

Choice of the suitablepart of tree slice for dendrochronologymeasurements.

Tree core

TREE CORE SAMPLINGTREE CORE SAMPLINGTw

o kind of tree sam

pling for dend

rochronology m

easurement

Tw

o kind of tree sam

pling for dend

rochronology m

easurement

Page 7: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

TREE RINGS MEASUREMENTTREE RINGS MEASUREMENT

Device for tree ring width measurement LINTAB in Gliwice Tree Ring Laboratory Device for tree ring width measurement LINTAB in Gliwice Tree Ring Laboratory Measurement precision is 0,01 mm.Measurement precision is 0,01 mm.

Page 8: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

Each tree should be measured at least in two radiuses.Each tree should be measured at least in two radiuses.The final results is The final results is meanmean from each radius of adequate tree ring measurement. from each radius of adequate tree ring measurement.

TREE RINGS MEASUREMENTTREE RINGS MEASUREMENT

Page 9: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

UNDER THE MICROSCOPEUNDER THE MICROSCOPE

Microscope view of pine (Microscope view of pine (Pinus SylvestrisPinus Sylvestris) ) sliceslice. This part shows. This part showsannual tree growths close to tree annual tree growths close to tree pithpith..

PithPith BarkBark

Page 10: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

PithPith BarkBark

UNDER THE MICROSCOPEUNDER THE MICROSCOPE

Page 11: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

In some cases tree growths are very thinIn some cases tree growths are very thin (e.g. (e.g. during the during the droughtdrought)). In this picture annual growth. In this picture annual growthss

with very small with very small widthwidth 0,3 – 0,8 mm are shown. 0,3 – 0,8 mm are shown.

PithPith BarkBark

UNDER THE MICROSCOPEUNDER THE MICROSCOPE

Page 12: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

REGISTRATION OF MEASUREMENT DATAREGISTRATION OF MEASUREMENT DATA

TSAPWin Basic 1.15b TSAPWin Basic 1.15b Program for registrationProgram for registrationtree measurement data.tree measurement data.

Page 13: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

EXAMPLES OF DENDROCHRONOLOGY MEASUREMENTEXAMPLES OF DENDROCHRONOLOGY MEASUREMENT

Stump 1_1 (r0 - 81 growth disappears)

0

0,5

1

1,5

2

2,5

3

3,5

4

4,5

5

0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150

calendar years

gro

wth

wid

th,

mm

width r0, mm width r1, mm width r2, mm avr

This pine slice comes from Niepołomice Forest, near Cracow.This pine slice comes from Niepołomice Forest, near Cracow.

Page 14: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

Stump 1_2

0

0,5

1

1,5

2

2,5

3

3,5

4

4,5

5

0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150

calendar years

grow

th w

idth

, mm

width r0, mm width r1, mm avr

This pine slice also comes from Niepołomice Forest, see the similarity of dendrochronology measurement on plots.This pine slice also comes from Niepołomice Forest, see the similarity of dendrochronology measurement on plots.

EXAMPLES OF DENDROCHRONOLOGY MEASUREMENTEXAMPLES OF DENDROCHRONOLOGY MEASUREMENT

Page 15: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

CHRONOLOGYCHRONOLOGY

FFor investigations of climate condition changes in the pastor investigations of climate condition changes in the past,, on the basis on the basisof dendrochronology measurementof dendrochronology measurement,, the calendar scale must be prepared. the calendar scale must be prepared.In this fact the tree rings from different trees are compared for constructionIn this fact the tree rings from different trees are compared for constructionof complete chronology.of complete chronology. In many cases tree rings width were correlated withIn many cases tree rings width were correlated withdendrochronology standard adequatedendrochronology standard adequatelyly for investigated tree species. for investigated tree species. ThisThiscorrelation allows to match the correlation allows to match the measurement of measurement of tree rings width to the calendartree rings width to the calendaryears. years.

Different sources of dendrochronology

material

Matching of tree rings width or indices

Chronology

Page 16: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

ISOTOPIC COMPOSITIONS IN TREESISOTOPIC COMPOSITIONS IN TREES

PrecipitationPrecipitation

AtmosphericAtmospheric CO CO22

LightLight1313CC

1818OO

1818OO

22HH

1515NN

3434SS

1000)(

)()(1213

1213121313

std

stdsample

CC

CCCCC

1000)(

)()(1618

1618161818

std

stdsample

OO

OOOOO

1000)(

)()(12

12122

std

stdsample

HH

HHHHH

1000)(

)()(1415

1415141515

std

stdsample

NN

NNNNN

1000)(

)()(3234

3234323434

std

stdsample

SS

SSSSS

Page 17: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

PREPARATION OF SINGLE TREE RINGSPREPARATION OF SINGLE TREE RINGS

Tree slice was cut by small chiselTree slice was cut by small chisel After After that,that, wood sample is cut wood sample is cutfor small piecesfor small pieces for chemical for chemical

treatmenttreatment

Chemical treatment in Soxlet’sChemical treatment in Soxlet’scolumns. Wood is in cotton bags.columns. Wood is in cotton bags.

Wood samples are rinsed inWood samples are rinsed inbenzene/alcohol mixture (2/1 proportion)benzene/alcohol mixture (2/1 proportion)

Resin and dyesResin and dyesremovesremoves

Boiling samples in laboratory bathBoiling samples in laboratory bathseveral hours in 90several hours in 90ºC for ºC for removing of removing of

rest of chemicalrest of chemical mixture. mixture. Next samples are dried. Next samples are dried.

Combustion to COCombustion to CO22

for GPC measurementfor GPC measurement

Benzene productionBenzene productionfor LSC measurementfor LSC measurement

cellulose productioncellulose productionfor stable isotope for stable isotope

concentration measurementconcentration measurement

Page 18: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

MEASUREMENT OF ISOTOPES RATIOS IN TREESMEASUREMENT OF ISOTOPES RATIOS IN TREES

COCO22 BenzeneBenzene CelluloseCellulose

GPCGPC LSCLSC

AMSAMS

1414CC

1313CC22HH

3434SS1818OO

1515NN

MASS MASS SPECTROMETERSPECTROMETER

Page 19: 14 C  13 C 2H2H2H2H  18 O Mariusz Fogtman and Anna Pazdur Silesian University of Technology Department of Radioisotopes

www.carbon14.plwww.carbon14.pl