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Sensitivity to H 2 0 2 and Glutathione Levels. 100. 100. 80. 80. 60. 60. % GSH content. % survival. 40. 40. 20. 20. 0. 0. w.t. D ynk. w.t. D ynk. NDP kinase Activity. Autophosphorylation Activity. Structure and Functional Analysis of YNK. 100. 80. 60. % survival. 40. - PowerPoint PPT Presentation
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% s
urv
iva
l
0
20
80
40
60
100
w.t. ynk%
GS
H c
ont
en
t
0
20
80
40
60
100
w.t. ynk
Sensitivity to H202 and Glutathione Levels
NDP kinase Activity
AutophosphorylationActivity
% s
urv
iva
l
0
20
80
40
60
100
w.t. ynk
Structure and Functional Analysis of YNK
ynk+ YNK
ynk+
P73H
ynk+
C118A
ynk+
H119F
Proteomix Analysis of ynk1 Knock-Out
w.t.
ynk
w.t. + H202 ynk + H202
CPY: PPIase
Arabidopsis
Accumulation of NDPK2 Transcript during Oxidative Stress
Enhanced NDP Kinase and Autophosphorylation Activity in Transgenic Plant Overexpressing NDPK2
ATP
GTP
w.t. transgenic
H2O2 : 0 5 10 0 5 10 (mM) 20 30 (mM)0 5 1020 300 5 10
w.t. transgenic
NDPK activity autophosphorylation activity
wild-type transgenic knock-out
w. t.
t. g.
k. o
.
rel
ativ
e fl
uo
resc
ence
(%
)
NDPK2 Regulates Cellular Redox Conditions
100
50
0
wild-type
NDPK2 Determines Phosphorylation of Two Putative Oxidative Stress Activated Proteins
42 kD
47 kD
wild-type
+ H202
transgenic
knock-out
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