Biomedical Research Retreat 2006 1

Biomedical Engineering Research at UNL Opportunities

Contacts:

Namas Chandra, Ph.D. P.E.Associate Dean-ResearchCollege of Engineering(402) 472-8310nchandra2@unl.edu

Becky DoleTechnical Assistant(402) 472-5600bdole2@unl.edu

Biomedical Research Retreat 2006 2

What is Biomedical Engineering?

Computations

Mathematics

ChemistryPhysics

Biology

Medicine

Human Behavior

Health

Novel• Biomaterials• Implants• Devices• Instruments• Informatics

• Better health

• Improved diagnostics

• Longer life

• Faster rehabilitation

Biomedical Research Retreat 2006 3

Areas of Expertise

• Tissue Mechanics (engineering)

– Mechanical, Engineering Mechanics, Chemical, and Biomolecular

• Medical Devices (surgical or otherwise)

– Mechanical, Engineering Mechanics, Industrial

• Biomechanics/Rehabilitation

– Mechanical, Engineering Mechanics, Industrial

• Image Analysis/Pattern Recognition

– Computer Science and Engineering

Biomedical Research Retreat 2006 4

Mechanical Devices

UNL: Carl NelsonUNMC: T. Hejkal

Infant Surgical TableGoal: Allow infant retinal surgery to be performed while seated with full rotation and tilt controls

Solution: Attachment for full-size surgical table; novel anticrimp attachments for hoses; adjustable safety-lock clamp

Goal: Eliminate frequent tool changes in MIS that waste valuable OR time and cause unnecessary trauma to patients

Solution: Multifunction tool; fuzzy logic guides preoperative tool setup

Multifunction Tool forMinimally Invasive Surgery (MIS)

UNL: Carl NelsonUNMC: D. Oleynikov

Biomedical Research Retreat 2006 5

Tissue Mechanics and New Ideas

• Make surgery easier, more effective, and more accessible

– Tool tracking in minimally invasive surgery

– Modular surgical robots

– Custom tools for tasks that are repetitive and mechanically difficult/time consuming

• Tissue Mechanics– Obtain mechanical properties

– Concerned with shear behavior, not just axial stress-strain

– Developing a very small measurement fixture

Test Set-up

UNL: Carl NelsonUNMC: J. Hammel

6

Miniature In Vivo Robots for Surgery

• Better– Miniaturized robots function entirely within

the body– Access to more surgical sites and organs– Improved visualization– Reduced incisions

• Faster– Limited set-up time– Additional tools can be added or removed quickly

• Cheaper– Mass produced– Nor hardware infrastructure (laptop enabled)– Disposable

UNL: Shane FarritorUNMC: Dmitry Oleynikov

Biomedical Research Retreat 2006 7

In Vivo Robots-Details

Mobile Camera

Pan/Tilt Camera

Biopsy

Biomedical Research Retreat 2006 8

Porcine In Vivo Mobility

Biomedical Research Retreat 2006 9

Worldwide Abundant Recombinant Hemophilia Therapy Made in the Milk of Transgenic Pigs:

Pharmacokinetic Properties for Intravenous and Oral Delivery Evaluated in Hemophilic Animals

Investigating Team

William H. Velander, Kevin E. Van Cott, Todd Swanson Dept of Chemical and Biomolecular EngineeringUniversity of Nebraska-Lincoln, Lincoln, NE

Stephan Butler and Julian Cooper, Progenetics LLC, Pembroke, VA

Paul E. Monahan, Gene Therapy CenterUniversity of North Carolina at Chapel Hill, NC

Oral Alpan and Polly Matzinger,NIH/NIAID, Bethesda Maryland

1987-------------1998 Fundamental studies2004-------------2007 Preclinical2007-------------2010 Clinical

Diverse Funding Source and Timeline

National Science FoundationCommonwealth of VirginiaAmerican Red CrossNIH/NIADProgenetics LLCMinistry of BrazilIndian Department of Biotechnology

Executive Council Meeting

P-1 / V-101

Pig Milk (FIX)

P-2 / V-102

Dilution Buffer 1

P-3 / MX-101

P-4 / BC-101

Cent / Fats remove

P-5 / V-103

Blending / 1:1

waste cent

P-8 / C-102

Heparin Capture

waste hep

P-10 / C-103

IONX

P-13 / DF-101

Conc / DiafP-14 / FDR-101

Freeze Drying / Vialswaste diaf 2

Lyophylized FIX

vent Lyoph

P-9 / V-104

P-15 / V-105

Dilution Buffer 2

P-7 / V-106

Viral Inactivation Buffer

P-16 / V-107

Viral Inact

P-17 / DE-101

Filtration

S-123

waste solid

Buffer 1

In Milk

anti-Vir

S-112

S-104

Buffer 2

P-19 / MX-102

S-105

S-102

S-114

P-20 / DF-102

Diafiltration

S-115

exch feed diaf1

exch feed diaf2

vent milk

vent antVi

vent buf 1

P-21 / MX-103

S-108

S-111S-137

vent

wa ionx

eq ionx

rg ionx

el ionx

wa hep

eq hep

rg hep

el hep

Fatsvent blend1

vent antVir

vent blend 2

S-109

w ionx

P-11 / DF-103

Diafiltration

exch feed diaf3

waste diaf 3

S-117

w diaf 1

P-6 / DPP-101

Fluid Flow

S-103S-106

P-18 / DPP-102

Fluid Flow

S-101

S-107

P-12 / C-101

FPLC IONX

wa ix2eq ix2

S-118

rg ix2

el-A ix2

el-B ix2

S-125

w ionx2

S-110

S-113

Transgenic Pig

High Purity Biologic formulation:Intravenous, Oral, Buccal, IN, IT Delivery

Recombinant Protein in Milk

Large Scale Purification Process

Pig Chromosome with Milk Production Gene through cloning or microinjection

Complex Recombinant Protein Production Process

Executive Council MeetingSaraf, UNL 2006rsaraf@unlnotes.unl.edu

Resolution is 20 mm which is 2-fold smaller than human finger;and 100 fold smaller than the currentElectronic MEMS devices.

Nano Touch Sensors Ravi Saraf, Chemical Engineering, rsaraf@unl.edu

Disphering cancer by texture: The surgeon has a resolution of our sensor is ~20 mm.

Giving surgeon the sensation of human touch during minimally invasive procedure:

Executive Council Meeting

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296 K (room temp.)

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A: add methanolB: remove methanolC: add methanolD: remove methanolE: air tightF: add isopropenolG: remove isopropenolH: add ethenol

pichia pastoris (yeast) on30 m long Au Nanoprticle Necklace: Metabolism Signal

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C E

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Single Electron Nanodevice to Measure Electrochemical Activity in a Single CellRavi Saraf, Chemical Engineering, rsaraf@unl.edu

• Combinatorial microarray with low false positive: Using the necklace as nano-electrode and combining with our combinatorial electrochemistry device, will allow us to measure perform microarray analysis using cells as the transduction agency.

• Live Bio-transistor: Use cell as a gating device to make electronics that is “intelligent” and “adaptable”.

• Power generation: Use the charge production form the cell (such as ATP cycle) to convert food directly to electricity.