2009 년 4 분기미토콘드리아 유전체

연구팀(mtDNA research

lab)

2009.12.30원양회관

정리 내용

연구원 동정 학술대회 참가 및 연제발표 투고 SCI 논문 연구과제 수행

연구원 동정 현재 신명근 교수님 , 김혜란 , 오지연 , 김환영 , 엄하영 , 안애자

연구원

2009. 1. 19 오지연 연구원 시작

2009. 02. 27 김혜란 박사학위 및 김명숙 , 조정애 석사학위수여

2009. 03. 02 엄하영 샘 석사학위 및 mtDNA 연구실연구원 시작

2009. 04. 03 2009 mtDNA 연구실 1 분기 모임

2009. 05. 28 김환영 연구원 시작

2009. 06. 01 안애자 샘 석사학위 및 mtDNA 연구실연구원 시작

2009. 6. 25 2009 mtDNA 연구실 2 분기 모임

2009. 9.30 2009 mtDNA 연구실 3 분기 모임

2009. 10.22 신명근 교수님 진단검사의학회 포스터 상 수상

2009. 12.3-8 신명근 교수님 , 김혜란박사 미국 ASH 학회 참가

2009. 12. 30 2009 mtDNA 연구실 4 분기 모임

• 세브란스병원 유전자은행 개소 심포지엄 “ Alteration of mitochondrial

genome and Proteome in chronic inflammatory diseases and hematolog-

ical malignancies: implication for the development of new molecular

biomarkers and targeted therapeutics” ( 신명근 )/2009.11.26 (

연세대학교 의과대학 종합관 331 호 ) 연자발표

• 심장질환 치료기술 개발 특성화 연구센터 “ Development of new molecular

biomarkers in chronic inflammatory disease and cancers using mito-

chondria genome and proteome analysis” ( 신명근 )/2009.11.24 (

전남대학교 의과대학 ) 연자발표

• The 18th KOGO Conference Omics to Personal Medicine “Oxidative

Stress, Mitochondrial Dysfunction and Mitochondrial Genome Aberration

in Chronic Inflammatory Tissue and Hematopoietic Diseases”( 신명근 )/

2009. 9.10 (Samsung Medical Center, Seoul, Korea) 연자발표

학술대회 참가 및 연제발표

• 09 추계 진단검사의학회 /2009. 10.22 ( 대구 인터불고 호텔 )

1) “Different proportion of leukemia stem cells according to

AML FAB subtypes and their lower single cell dividing prop-

erty” ( 신명근 , 김혜란 ) 포스터발표

2) “Association of the prohibitin 3’untranslated region poly-

morphisms with increased risk of AML”( 신명근 , 김혜란 )

포스터발표

3) “Remarkable discrepancy between allele-specific PCR and

real-time PCR melting curve analysis in detection rate of

JAK2 V617F mutation”( 신명근 , 김혜란 ) 구연발표

• 09 51th ASH 학회 ( 미귝 , 뉴올리언즈 )

1) “AML FAB subtypes shows different proportion of leukemia initiat-

ing cell which has lower single cell dividing property”( 김혜란 ,

신명근 )

2) “Increased mitochondrial mass and mitochondrial DNA copy

numbers are biomarkers for benzene exposure in hematopoietic

tissue and leukemia cells”( 신명근 , 엄하영 , 김혜란 )

3) “Profound length heteroplasmic alterations in mitochondrial DNA

control region from primary AML cells: implication of impaired

mitochondrial replication and demonstration of ‘vicious cycle’

hypothesis” ( 신명근 , 김혜란 )/ 포스터발표

4) “The Prohibitin 3’ untranslated region polymorphisms are

associated

with AML susceptibility” ( 김혜란 , 신명근 )

 

• 1st Korean Myeloma Workshop “MRD markers of multi-

ple myeloma”( 신명근 )/ 2009. 6. 20 ( 화순전남대병원 대강당 )

연자발표

• 3rd International WHO Conference on Children’s

Health and the Environment “ Short-term effect and profil-

ing of mitochonrial genomic instability in leukemia cell lines

ex vivo after benzene exposure”( 엄하영 , 신명근 , 김혜란 )/

2009.6.7-10(Busan Bexco) 포스터발표

• 2009 년 제 50 차 대한혈액학회 춘계학술대회

1)“Benzene Exposure was Associated with Increased Mito-

chondrial Mass in Leukemia Cell Lines” ( 엄하영 , 신명근 ,

김혜란 , 오지연 )/ 2009. 5. 29 ( 서울 홍은동 그랜드힐튼호텔 )

포스터발표

2) “Clonality Assay in the Lymphoid Neoplasm”( 신명근 )/

2009. 5. 30 ( 서울 홍은동 그랜드힐튼호텔 ) 연자발표

3)“Demonstration of Multiple Myeloma Cell-specific mtDNA

Mutations in Bone Marrow Cells at an Initial Diagnosis” ( 신명

근 , 김혜란 )/ 2009. 5. 29 ( 서울 홍은동 그랜드힐튼호텔 )

구연발표

4)“The First Korean Case of Aplastic Anemia in Cohen Syn-

drome Documented by Mutation Analysis” ( 김혜란 , 신명근 )/

2009. 5. 29 ( 서울 홍은동 그랜드힐튼호텔 ) 포스터발표

5)“Spectrum of Length Heteroplasmic Aberrations in Mito-

chondrial DNA from Primary AML Cells: Implication of Impaired

Mitochondrial Replication and ATP Synthesis” ( 신명근 , 김혜란 )/

2009. 5. 29 ( 서울 홍은동 그랜드힐튼호텔 ) 포스터발표

6) SET-NUP214 Fusion Gene Contributed to the Development of

AML with Minimal Differntiation and T-ALL”( 신명근 , 김혜란 )/

2009. 5. 29( 서울 홍은동 그랜드힐튼호텔 ) 포스터 발표

7) “Distribution and Proportion of AML Stem Cells to FAB Classifi-

cation and CD34+ Fraction in Bone Marrow Specimens and

Lower Dividing Property of AML Stem Cells in Single Cell Sus-

pension Culture System”( 김혜란 , 신명근 ) / 2009. 5. 29( 서울

홍은동 그랜드힐튼호텔 ) 구연발표

• 대한진단검사의학회 2009 년 춘계 심포지엄 Laboratory

Medicine: Back to the Basics “Myeloproliferative Neo-

plasms, MPN”( 신명근 ) / 2009. 4. 9(W Seoul-Walkerhill Ho-

tel ) 연자발표

2009 4 분기투고 SCI 논문

1) Choi HJ et al. Detection of putative T cell clones using T cell receptor β

chain gene clonality assay in Korean patients with aplastic anemia. Korean

J Lab Med. SCIE-indexed 2009 Aug;29(4):269-76.

2) Kim HR et al. Alteration of mitochondrial DNA sequence and copy number

in nasal polyp tissue. Mitochondrion. SCI-indexed 2009 Sep;9(5):318-25.

3) Kim DW et al. Incidence and Causes of Eosinophilia from the Patients in a

University Hospital. Korean J Lab Med. SCIE-indexed 2009 Jun;29(3):185-

93

4) Shin MG et al. Application and usefulness of multiplex reverse transcrip-

tion-polymerase chain reaction for simultaneous screening of 28 transloca-

tions and chromosomal aberrations in leukemia. Korean J Lab Med, SCIE-

indexed 2009, under revision

★ 주저자 및 교신저자

★ 공저자

1) Kim DW, Shin JH, Kee SJ, Kim SH, Shin MG, Suh SP, Ryang DW.“Evaluation of VITEK-2 Antifungal Susceptibility Test (AST-YS01) for Candida Species Isolates in Korea. ” Korean J Lab Med. 2009 Sep;12(3):122-128.

2) Kim SH, Cho D, Kee SJ, Shin MG, Shin JH, Suh SP, Ryang DW.“Allele-related Variation in Minisatellite Repeats Involved in Transcription of the ABO Gene in Korean Blood Donors.”

Korean J Lab Med. 2009 Sep;44(3):127-132.

3) Moon CJ, Shin JH, Jeong ES, Kee SJ, Kim SH, Shin MG, Suh SP, Ryang DW.“Central Venous Catheter-Related Microbacterium Bacteremia Identified by 16S ribosomal RNA Gene Sequencing .” 

Korean J Lab Med. 2009 Jun;12(2):97-101.

4) Yun HK, Kim SH, Cho D, Kee SJ, Shin MG, Shin JH, Suh SP, Ryang DW.“Comparison of Two Sputum Processing Methods for Detecting Mycobacterium tuberculosis by Culture and PCR: Universal Sample Processing(USP) and NALC-NaOH Methods.” Korean J Lab Med.  2009 Jun;12(2):67-71.

5) Kee SJ, Kim SM, Kim SH, Shin MG, Shin JH, Suh SP, Ryang DW. “Multiplex PCR Assay for Identification of Mycobacterial Species Iso-

lated from Liquid Cultures.” Kor J Lab Med (SCIE) 2009, 45(1):19-26.

6) Moon CJ, Shin JH, Kim DW, Kee SJ, Kim SH, Shin MG, Suh SP, Ryang DW.

“Species-Specific Differences in Rhodamine 6G Accumulation of Can-dida Isolates Detected by Flow Cytometric Analysis.”

Korean J Lab Med. 2009 Apr;29(2):127-134.

7) Cho SH, Huh JW, Kim YJ, Shin MG, Kim HR. “APC Mutation Spectra in Sporadic Colon Cancer Tissue among Korean.” Kor J Lab Med (SCIE) 2009, 76(3):159-167.

8) Kim MN, Shin JH, Sung H, Lee K, Kim EC, Ryoo N, Lee JS, Jung SI, Park KH, Kee SJ, Kim SH, Shin MG, Suh SP, Ryang DW.

“Candida haemulonii and closely related species at 5 university hospitals in Korea: identification, antifungal susceptibility, and clinical features.” Clin Infect Dis. 2009, 15;48(6):e57-61.

9) Kim SH, Shin JH, Kim EC, Lee K, Kim MN, Lee WG, Uh Y, Lee HS, Lee MK,

Jeong SH, Jung SI, Park KH, Lee JS, Shin MG, Suh SP, Ryang DW.

“The relationship between antifungal usage and antifungal suscepti-bility in clinical isolates of Candida: a multicenter Korean study.”

Med Mycol. 2009, 47(3):296-304. Epub 2008 Jul 30.

10) Song JW, Shin JH, Kee SJ, Kim SH, Shin MG, Suh SP, Ryang DW.

“Expression of CgCDR1, CgCDR2, and CgERG11 in Candida glabrata biofilms formed by bloodstream isolates.” Med Mycol. 2009;47(5):545-8.

★ Manuscript prepara-tion1) Association of allelic expression imbalance of JAK2

V617F mutant and phenotypic diversity in BCR-ABL negative myeloproliferative neoplasma

2) Direct confirmation of silent property of AML stem cells using single cell culture system

3) Aberrant expression of prohibitin in primary AML cells and their implication of treatment monitoring, progno-sis and therapeutic approach

4) Spectrum of mitochondrial genome instability of pri-mary AML cells and their clinical and pathophysiologic implication

5) Colon and stomach cancer-specific mitochondrial DNA mutations

6) The change of Mitochondrial copy number is possible biomarker of benzene exposure in leukemia cell lines

연구과제 수행 1) 단독연구과제

i) 09 과학재단 특정기초

ii) 09 보건의료기술사업 과제

2) 세부연구과제

i) 환경성 질환연구센터 세부과제

모두의 앞날에 무궁한 발전이

있도록

우리 모두 화이팅 !!!

감사합니다 ~

2009 미국혈액학회 과장님 독사진입니다 .

미토콘드리아 유전체팀

미녀들입니다 .