PATENT ABSTRACTS 105

The sensor comprises an outer tube having on its one end a gas-permeable membrane and a pH electrode disposed in the outer tube and supporting a spacer of poor hydro- phobicity so as to be positioned between the distal end of the electrode and the mem- brane. For measurement of carbon dioxide, a liquid containing sodium bicarbonate is poured into the outer tube to form a layer of electrolyte at the spacer. and the outer tube is mounted on a fermentor. The pH elec- trode can be removed from the outer tube which is attached to the fermentor, and a membrane protecting rod can be inserted into the outer tube for sterilization of the membrane. The gas-permeable membrane can easily be replaced.

4376165

METHOD OF PREPARING AN ENZYME-LABELED LIGAND

FOR USE IN SPECIFIC BINDlNG ASSAYS AND THE LABELED

CONJUGATE PRODUCED THEREBY

William Hornby. David L. Morris, Berk- shire, United Kingdom. assigned to Miles Laboratories Inc

A method of preparing an enzyme-labeled ligand for use in specific binding assays for determining said ligand or a specific binding partner thereof, by covalently coupling said ligand or a binding analog thereof to an

organic prosthetic group and combining the resulting coupled product with an apoen- zyme to form a conjugated enzyme complex with said prosthetic group in said coupled product. Preferably. the conjugated enzyme complex in the resulting labeled conjugate exhibits holenzyme activity which is affected by binding of said labeled conjugate by a specific binding partner of said ligand. Preferred prosthetic groups are flaving mononucleotide. heme, and. especially. flavin adenine dinucleotide, with preferred

apoenzymes being apoperoxidase and. especially, apoglucose oxidase.

4375510

SELECTIVE MEDIUM COMPOSITION AND METHOD FOR THE DETECTION OF ACTINOMYCES VISCOSUS AND

ACTINOMYCES NAESLUNDII

Harold Jordan, assigned to Forsyth Dental Infirmary for Children

A selective medium composition for the growth and detection of Actinomyces visco- sus or Actinomyces naeslundii. which com- position comprises: a solid medium selective to induce the growth of Actinomyces visco- sus or Actinomyces naeslundii; a nutrient agent to induce the substantial growth of Actinomyces viscosus or Actinomyces naes- lundii; a cadmium compound; a fluoride compound; a flavin compound; and the cadmium compound, the fluoride compound and the flavine compound all present in a concentration sufficient to inhibit the sub- stantially full growth of interfering microor- ganisms. but in a concentration insufficient to inhibit the substantial growth or Action- omyces viscosus or Actinomyces naeslundii.

4375401

ELECTROPHORESIS SYSTEM

Nicholas Catsimpoolas

106 PATENT ABSTRACTS

An improved electrophoresis system for simple, rapid and reproducible separations of biological materials such as proteins. nucleic acids, cells, bacteria. cell organelles. etc. is described. The system consists of se\‘- era1 subsystems including a first dimensional system having a multi-column rotatable carousel with automatic density gradient maker and sample deposition, a unique fraction collector cassette which can serve as a sample applicator for the second dimen-

sion electrophoresis and a slab-gel second- dimensional electrophoresis system. The total system operation is integrated by micropro- cessor control. Flexibility in the system design and operation allows for running in an ana- lytical or preparative mode at the discretion of the system operator.

4375399

MOLECULE SELECTIVE SENSOR FOR INDUSTRIAL USE AND

PROCEDURE FOR ITS PREPARATION

Jeno Havas. Gera Nagy, Emm PorjesT. Erno Pungor, Budapest. Hungary, assigned to Radelkis Elektrokemiai MisTergyarto SzovetkeTet

A modecule selective sensor having two permeable membranes in contacting rela- tionship with each other, one membrane being a protein membrane’found in nature. as for example. hog intestine membrane. air

bladder of fish, epidermal section 01 mam- mals and reptiles. etc. and the other mem- brane being the reaction product of acryla- mide, polyvinylalcohol. an enzyme, bovine albumin and glutaric aldehyde.

4374981

ULTRAFILTRATION OF FERMENTATION BROTH

CONTAINING NUCLEOSIDES TO SEPARATE INOSINE AND

GUANOSINE FROM THE BROTH

Masahiko Tsuda, Kaztihiko Ohta, Kiyoshi Nara, Kobe, Japan, assigned to Takeda Chemical industries Ltd

lnosine and /) or guanosine can be separated from fermentation broth containing microb- ial cells and high-molecular-weight substan- ces by heat-treating said broth at 90 degrees to I IO degrees C., followed by subjecting to ultrafiltration. The present process is advan- tageously carried out in the industrial use of ultrafiltration.

4374013

OXYGEN STABILIZED ENZYME ELECTRODE

Sven-Olof Enfors, S I91 41 Sollentuna. Sweden