4554253

PATENT ABSTRACTS

A P P A R A T U S F O R S Y N T H E S I Z I N G A D E N O S I N E - 5 ' -

T R I P H O S P H A T E

Kazutom lmahori, Tatsuo Iwasaki, Hirosh Nakajima, Hitoshi Kondo, lsao Tomioka, Masaru Kashima, Toshihiko Tsukamoto, Kakinokisaka, Meguro ku, Tokyo, Japan as- signed to Imahori Kazutomo; Kenkyusho Rikagaku; Unitika L

An apparatus for converting into ATP which comprises an enzyme reactor, a source of AMP supply, a source of phosphoric acid donator supply, variable fluid sending apparatus, an automatic sampling apparatus and an analyzing apparatus for the reacting solution, an arith- metic control apparatus, and a recovery ap- paratus. According to this apparatus, conversion from AMP or ADP into ATP can be effectively carried out and ATP conversion can be kept at substantial 100% over a long period of time. The device makes it possible for ATP to be used more and more in future as an energy source for bioreactors and as medicines because the ATP will be more readily available and less expensive.

4555487

M E T H O D F O R C U L T I V A T I O N O F P S E U D O M O N A S B A C T E R I A

Hideak Yamada, Koitchir Ryuno, Kyoto, Japan assigned to Nitto Kagaku Kogyo Kabushiki Kaisha; Hideaki Yama

Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained in a high yield by adding to a culture medium at least one amide compound selected from the group consisting of acrylamide, methacrylamide, crotonamide, and n-butyramide in the prepara- tion of cells of bacteria having nitrile hydratase activity by cultivating Pseudomonas bacteria capable of producing nitrile hydratase.

4 5 ~ 5 ~

I M M O B I L I Z E D E N Z Y M E S

Luis C Calvo assigned to Germaine Monteil Cosmetiques Corp

A cosmetic composition is provided for the removal ofsebum exudate from the skin. The

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composition comprises immobilzed enzymes in cosmetically acceptable vehicles for topical application. The immobilized enzymes are lipolytic lipases, proteolytic proteases and en- zymes for the breakdown of sugar oligomers from glucoproteins in the skin exudates. The en- zymes may be present individually or in any desired combination. The enzymes are im- mobilized in known manners by chemical and or physical means and are released from im- mobilization upon application to the skin.

4556637

I M M O B I L I Z E D C H O L I N E S T E R A S E E N Z Y M E

P R E P A R A T I O N S A N D A P R O C E S S F O R T H E P R E P A R A T I O N

T H E R E O F

Laszlo Szajani Bela Boross, Kamilla Kovacs, Budapest, Hungary assigned to Reanal Finom- vegyszergyar

Immobilized cholinesterase enzyme prepara- tions are prepared by treating a polymeric resin, built up from acrylic acid and/or methacrylic acid and acryl amide and/or methacryl amide monomers with an acryl or allyl type cross- linking agent and containing at least 0.1 meq/g o f - C O O H functional groups, with a car- bodiimide derivative which is soluble in water or is soluble in an organic solvent at temperatures below 0 degrees C., applying a solution of cholinesterase enzyme with a pH of 4.5 to 8.5 to the resulting activated support, washing the resulting product, and drying it if desired.

4559307

T R E A T M E N T O F Y E A S T C E L L S W I T H P R O T E O L Y T I C E N Z Y M E S

Thomas R Hopkins assigned to Phillips Petro- leum Company

Functional protein having reduced nucleic acid content is produced without initial denaturation of the protein by contacting undenatured yeast cells with an alkaline protease at a temperature of about 20 degrees C. to 40 degrees C. for about 2 minutes to 2 hours at a pH of about 8 to 1 I. The yeast cells are preferably Pichia pastoris and the alkaline protease is preferably from Bacillus lichenformis.