160 PATENT ABSTRACTS

4629691 4629694

T R I C Y C L I C A N T I D E P R E S S A N T C O N J U G A T E S W I T H A N T I G E N S

A N D E N Z Y M E S

D E T E C T I N G A N D D I S T I N G U I S H I N G B E T W E E N

P L A S M I N O G E N A C T I V A T O R S

Christine G Collins, Marcel R Pirio, Prithipal Peter C Harpel assigned to Cornell Research Singh assigned to Syntex (U S A ) Inc "Foundation Inc

Tricyclic antidepressant functionalized com- pounds are provided for conjugation through a side chain to antigenic compounds, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies and together with the enzyme con- jugates find particular use in immunoassays for the determination or detection of the total amount of tricyclic antidepressants present in a sample.

4629692

I M M U N O A S S A Y F O R N O N E N Z Y M A T I C A L L Y

G L U C O S Y L A T E D P R O T E I N S A N D P R O T E I N F R A G M E N T S A N

I N D E X O F G L Y C E M I A

This invention relates to a method for deter- mining fibrinolytic activation in human blood and to a method to distinguish between tissue (e.g. vascular) plasminogen activator and urokinase-like plasminogen activator contribu- tions to fibrinolytic activation. Broadly, the method of the invention relates to detecting the level of plasmin-plasmin inhibitor complexes in blood plasma samples before and after clotting thereof; a greater increase in the complex level in the clotted sample as compared to non-clotted samples indicating the presence of circulating tis- sue plasminogen activator, with substantially identical levels of increase of complex in both the clotted and unclotted samples indicating the sole presence of urokinase-type plasminogen ac- tivator in the sample.

Kenneth J Dean assigned to Miles Laboratories Inc

An immunoassay method and reagent system for determining nonenzymatically glucosylated proteins and protein fragments in a biological fluid based on the specific binding of such pro- teins and fragments with anti(Amadori- rearranged glucose), e.g., antibodies which selectively recognize the rearranged deoxy- fructose form of glucose resulting when proteins are nonenzymatically glucosylated. The anti- bodies are raised against an immunogen comprising an immunogenic carrier material bearing 1-deoxy-l-fructosyl residues or con- formers of such residues. Measurement of none- nzymatically glucosylated proteins and fragments thereof provides a useful index of blood glucose levels.

4629693

S E N S I T I V I T Y IN F L U O R E S C E N C E A S S A Y S IN I C T E R I C S A M P L E S

Pyare Khanna assigned to Syntex (U S A ) Inc

Enhanced sensitivity in fluorescent assays em- ploying NADH or NADPH is obtained, par- ticularly in icteric samples, by employing excitation light at a wave length range below ab- out 400 nm and reading emitted light above ab- out 500 nm.

4 ~ 9 6 ~

P E P T I D E D E R I V A T I V E S A N D U S E T H E R E O F A S S U B S T R A T E S F O R

Q U A N T I T A T I V E L Y A S S A Y I N G E N Z Y M E S

Lars Svendsen, Reinach BL, Switzerland as- signed to Pentapharm A G

Peptide derivatives of formula See Patent for Chemical Structure wherein R 1 represents a sub- stituted amino group which is capable of being split off enzymatically with formation of a colored or fluorescent compound RI-H. The said peptide derivatives are used for quantita- tively assaying the enzyme Cl-esterase. The as- saying is carried out by reacting a medium which contains Cl-esterase or in which the latter is for- med or consumed with a peptide derivative as defined above and measuring photometrically, spectrophotometrically, fluorescence- spectrophotometrically or electrochemically the quantity of split product R I-H released per time unit by the catalytic hydrolytic action of the said enzyme on the peptide derivative.