2174 PATENT ABSTRACTS

vention also discloses Xanthomonas mutants electrophoresis gel and voltage is applied across which produce the polysaccharide polymer but the gel to cause subgroups of the fragments to which do not produce xanthan gum. Methods of propagate along the columns at a velocity which preparing the polysaccharide polymers and of is a function of the length of the fragments in the their use are also described, subgroup. Radiation detectors are used to detect

the subgroups of fragments as they pass a pre- determined position along the gel column, and

4868749 data corresponding to the order in which the subgroups of fragments are detected is stored in

S I G N A L P R O C E S S I N G M E T H O D a memory. Such data is then used to determine I N A U T O R A D I O G R A P H Y the bases sequence of the molecules.

Tsutomu Kimura, Kazuhiro Hishinuma, Kaisei, 4870006 Japan assigned to Fuji Photo Film Co Ltd

A signal processing method in autoradiography A N T I G E N I C M A T E R I A L F O R A for determination of the base sequence of DNA C H A G A S ' D I S E A S E D E T E C T I O N or a EINA fragment, employing groups of S Y S T E M radioactively labeled base specific cleavage pro- ducts or mixtures thereof obtained by Elizabeth A Dragon, Stacey Sias assigned to specifically cleaving the DNA or DNA fragment Codon labeled with a radioactive element and resolved one-dimensionally in parallel relation to each T. Cruzi polypeptide antigens that react with other to form resolved rows on a support serum from chagasic individuals and does not medium. An autograph is obtained having loca- cross-react with serum from uninfected in- tional information on groups of radioactively dividuals or individuals infected with related labeled cleavage products contained in the rows parasites such as Leishmania is described. The on the support medium. An electrical digital DNA from T. Cruzi culture trypomastigotes and signal corresponding to the autoradiograph is epimastigotes coding for antigenic material then generated. Sampling points in each resolved having a molecular weight of 70 kd is identified, row of the digital signal are next detected, sequenced, and inserted into a cloning vector, Reference sampling points are then determined which, in turn, is inserted into a host cell line. The in a plurality of reference rows which are either expressed polypeptide is immunologically reac- directly provided on resolved row adjacent to the rive with sera from Chagas' disease infect pat- reference row to identify sampling points in adja- ients. The cloned gene for the 70 kd polypeptide cent resolved rows. Sampling points in the is expressed and purified and a diagnostic test for remaining non-reference rows are identified for Chagas' disease comprising the synthesized determination of the base sequence of the DNA polypeptide is described. or DNA fragment.

487OOO8 4870004

S E C R E T O R Y E X P R E S S I O N I N A P P A R A T U S A N D M E T H O D O F E U K A R Y O T E S

A N A L Y Z I N G N U C L E I C A C I D M O L E C U L E S Anthony J Brake assigned to Chiron Corpora-

tion Thomas J Conroy, Martin H Graham assigned to Autoseq Inc Methods and compositions are provided for

producing polypeptide sequences in high yield Apparatus and method of sequencing nucleic by employing DNA constructs, wherein the acid molecules, including DNA and RNA DNA sequence encoding from the polypeptide molecules. Several copies of the molecules are of interest in preceded by a leader sequence and labeled with a radioactive tracer and divided into processing sequence for secreting and processing separate groups. The groups of molecules are said polypeptide. In this manner, the mature then each chemically cleaved to produce labeled polypeptide of interest may be isolated from the fragments of varying length which terminate in nutrient medium substantially free of major predetermined bases. The groups of fragments amounts of other proteins and cellular debris. are then introduced into separate columns of The yeast strain S. cerevisiae ABI03 (pYEGF8)

PATENT ABSTRACTS 375

was deposited on Jan. 5,1983, at the A.T.C.C. 4870015 and given accession No. 20658. The plasmid pY alphaEGF23 (pABII4-pCI/I) was deposited at M E T H O D A N D C O M P O S I T I O N the A.T.C.C. on Aug. 12, 1983, and given Acces- F O R P R O D U C I N G L E C T I N I N sion No. 40079. M I C R O O R G A N I S M S

Leslie M Hoffman assigned to Lubrizol Genetics 4870009 Inc

M E T H O D O F O B T A I N I N G G E N E Methods and compositions for the construction P R O D U C T T H R O U G H T H E of a recombinant vector containing plant or

G E N E R A T I O N O F T R A N S G E N I C animal lectin DNA sequences, such vector being A N I M A L S capable of being replicated, transcribed and

translated in single cell hosts. The gene coding for the plant or animal lectin may be inserted

Ronald M Evans, Richard Palmiter, Ralph L into an expression vector which enables efficient Brinster assigned to The Salk Institute for Bio- production of the lectin protein. logical Studies

Mammalian genes that encode hormones are cloned and linked to strong promoter DNA 4870017 sequences. The linked sequences are inserted in plasmids for amplification in prokaryotic cells, B A C T E R I A L M E T H I O N I N E and multiple copies of the linked sequences are N - T E R M I N A L P E P T I D A S E excised therefrom. Linked sequences are sub- sequently microinjected into fertilized eggs and Arie Ben-Bassat, Keith A Bauer, Shing Chang, the fertilized eggs are implanted into pseudo- Sheng-Yung Chang assigned to Cetus Corpora- pregnent females of the same species. As a result, tion transgenic animals are born having the linked sequences incorporated into their genomes and Methods of obtaining N-terminal methionine- expressing the gene-encoded hormone. Because free proteins are described. The methods employ multiple copies of the linked sequences are fie- a novel enzyme, E. coli methionine amino- quently inserted and because production of the peptidase either in vitro or in vivo. For in vivo hormone is not limited to certain organs, as is the application, plasmid-borue DNA encoding the case with most endogenous hormones, the trans- peptidase is transformed into a bacterial host genic animals produce substantial amounts of which produces the desired protein. the hormone. Hormone can be harvested from the living animal (and from its hormone- producing progeny) by extracting fluid, such as 4870023 blood serum or ascites fluid, on a regular basis.

R E C O M B I N A N T B A C U L O V I R U S O C C L U S I O N B O D I E S I N

4870014 V A C C I N E S A N D B I O L O G I C A L I N S E C T I C I D E S

C L O N I N G O F T H E R M O L A B I L E G L U C O A M Y L A S E G E N E I N T O Malcolm J Fraser, Elliot Rosen, Victoria A

S A C C H A R O M Y C E S C E R E V I S I A E Ploplis assigned to American Biogenetic Sciences Inc

Judy A Eratt, Anwa Nasim, Ottawa, Canada as- signed to Canadian Patents and Development The present invention is directed to recombinant Ltd baculoviruses which encode fusion polyhedrin

proteins capable of forming occlusion bodies This invel:ntion concerns a glycoamylase gene containing foreign peptides. The recombinant cloned into the yeast Saccharomyces cerevisiae, baculoviruses of the invention are formed by in- method for cloning such a gene into such yeasts sertion into or replacement of regions of the and cloning vehicles containing such a gene, polyhedrin gene that are not essential for occlu- suitable for use in Saccharomyces cerevisiae, sion body formation, with foreign DNA flag- Yeast containing a glucoamylase gene are of ments by recombinant DNA techniques. The potential use in the brewing industry, recombinant occlusion bodies produced in ac-