PATENT ABSTRACTS

to fluorescence with an output spectrum charac- teristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA frag- ment can be reconstructed.

4962045

T I M E - R E S O L V E D F L U O R I M E T R I C D E T E C T I O N O F

L A N T H A N I D E L A B E L E D N U C L E O T I D E S

Enrico Picozza, Steven S Saavedra assigned to The Perkin-Elmer Corporation

Nucleotides conjugated with a chelating agent and labeled with a lanthanide, especially ter- bium, are readily detected by time-resolved fluorimetry during and following gel elec- trophoresis. The labeled nucleotide conjugates are very stable, highly fluorescent with a long radiative lifetime and remain fluorescent during dilution and during gel electrophoresis and since an enhancement solution is not required for detection, the labeled nucleotide conjugates can be detected on-line, if desired.

97

resulting sensitized latex particles combine at- tributes of each monomer to provide utility in difficult protein systems to form latex anti- gen/antibody complexes. The products of the in- vention may be used in a variety of diagnostic applications.

4963355

P R O D U C T I O N O F A N T I B O D Y C A T A L Y S T S

Peter S Kim, Neville Kallenbach assigned to Igen Inc

Process for producing an antibody catalyst for a chemical reaction. Chemical reactions in which the antibody catalyst can be used are also dis- closed.

4963486

D I R E C T F E R M E N T A T I O N O F C O R N T O L ( + ) - L A C T I C A C I D BY

R H I Z O P U S O R Y Z A E

4962154

L A T E X - A N T I G E N - A N T I B O D Y C O M P L E X E S F O R

I M M U N O A S S A Y

Douglas Pollock, Charles J McDonald, William E Cohrs, John Reno assigned to The Dow Chemical Company

A latex partice comprising a vinyl aromatic monomer and an vinyl acrylate ester monomer which is modified by a protein binding modifying monomer to facilitate the binding of a protein via coupling techniques. These latex par- ticles are useful in diagnostic test applications. The vinyl acrylate ester monomer and a vinyl aromatic monomer are dispersed in a ratio bet- ween 1:3 to 3:1 of vinyl acrylate ester monomer to vinyl aromatic monomer in an aqueous phase containing a protein binding modifying mono- mer. The protein modifying monomer can be a vinyl carboxylic acid, such as a acrylic acid in a concentration of from about 0.5 percent to ab- out 10 percent of the monomers. The dispersion is subjected to emulsion polymerization to pro- vide a latex particle in a size range from 0.1 to 1 micron. The latex particle is sensitized with an antibody or antigen via coupling techniques. The

Yong Hang assigned to Cornell Research Foun- dation Inc

L( + )-lactic acid is produced in high yield by a direct one step fermentation of renewable bio- mass such as ground corn using Rhizopus oryzae in the presence of calcium carbonate. A kilogram of crushed corn yields over 350 grams high purity L(+ )-lactic acid.

4963490

P O R O U S I N O R G A N I C M E M B R A N E S U P P O R T A N D

M E T H O D

Stephen J Churchouse, Elizabeth M Scamans, Banbury, United Kingdom assigned to Alcan International Limited

A method of growing or maintaining mam- malian cells comprises applying a culture of the cells to a surface of a porous inorganic mem- brane support. The support is preferably a trans- parent anodic aluminium oxide membrane. The support carrying cells (44) growing or being maintained thereon may comprise a short tube (30) and a disc (32) oftbe porous inorganic mem-