Abel Onyango, PhD student Kenya AIDS Vaccine Initiative-University of Nairobi

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EFFECTS OF SEXUAL ACTIVITY ON IMMUNE ACTIVATION AMONG HIV-EXPOSED SERONEGATIVE FEMALES AT PUMWANI CLINIC, NAIROBI. Abel Onyango, PhD student Kenya AIDS Vaccine Initiative-University of Nairobi Dept. of Medical Microbiology. OUTLINE. Background Problem statement Justification - PowerPoint PPT Presentation

Text of Abel Onyango, PhD student Kenya AIDS Vaccine Initiative-University of Nairobi

  • EFFECTS OF SEXUAL ACTIVITY ON IMMUNE ACTIVATION AMONG HIV-EXPOSED SERONEGATIVE FEMALES AT PUMWANI CLINIC, NAIROBIAbel Onyango, PhD studentKenya AIDS Vaccine Initiative-University of NairobiDept. of Medical Microbiology

  • OUTLINEBackgroundProblem statementJustificationResearch questionsHypothesisObjectivesMethodsTime-plan

  • OVERALL OBJECTIVETo determine the effects of sex work on systemic T cell activation in HIV-Exposed Seronegative (HESN) female sex workers at Pumwani Clinic, Nairobi

  • BACKGROUNDIn Kenya, new infections =104,137 (91,000=Adults)

    Sexual transmission accounts for approx. 93% of new HIV infections; with heterosexual bearing 77% cases

    About 10-15% population resist HIV (Shearer and Clerici, 2010)4

  • NEW HIV INFECTIONS-KENYA 2011

  • HESN FEMALE SEX WORKERS

    Definition: Previous =3 or more years Current definition >7yrs of follow up with normal CD4 and seronegative and PCR result for HIV6

  • MODEL OF THE SEROCONVERSIONFowke et al., 19967

  • CHARACTERISTICS OF HESNLower levels of activated T cells in the peripheral blood

    Increased levels of peripheral regulatory T cells

    Decreased levels of inflammatory cytokine production8

  • PROBLEM STATEMENT

    Global vision for zero new infections by 2015 heightens need for effective preventive HIV and treatment strategies

    Sexual transmission is the primary driver of Kenyas HIV infections

    9

  • JUSTIFICATION

    Varying susceptibility to HIV between high risk individuals has not been clearly elucidated

    Role of menstrual cycle on immune activation is unclear

    Urgent need for prevention strategies and/or new treatment options10

  • RESEARCH QUESTIONS

    Does persistent sex-driven stimulation induce Immune Quiescent state among HESN female sex workers?

    What effect does menstrual cycle have upon immune activation among HESN female sex workers during abstinence?11

  • HYPOTHESISHESN individuals downregulate sex work-driven immune activation more efficiently than HIV susceptible controls, which provides protection against subsequent HIV exposure

  • METHODOLOGYData analysis Blood draws and lab analysis

    Blood CD69, CD38, CCR5,CCR7,HLA-DR levels Data analysis Analysis will be done using paired T-test and ANOVAto compare differences in means of T cell activation determinants

  • FLOW CYTOMETRY PANELCD3 PECy5 CD4 FITC CD8 V500 CCR7 PE-CF594 CD45RA Alexa700 CCR5 V450 HLA-DR APC-H7CD161 APCCD95 PE, CD69 PE-Cy7

  • SO WHAT???Clear understanding and potential discovery of unique protective mechanisms among HESN individuals may provide opportunity for further scientific knowledge and perhaps develop new preventive interventions or treatment options for HIV 15

  • ACKNOWLEDGEMENTSDr. Keith Fowke,PhD Prof. Omu Anzala, MD, PhDDr. Julius Oyugi, PhDJulie Lajoie,PhDGenevieve Boily-Larouche,PhDLyle McKinnon,PhD

    UOMIDRCUNITID staffsKAVI-UON staffs