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Adenovirus vectors Gene Therapy-2008. Virology 1 Infectivity Infectivity How do you measure it? How…
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Infectivity Measure by infecting dilutions of the virus stock on permissive (e.g. 293) cells. Measure by infecting dilutions of the virus stock on permissive (e.g. 293) cells. Plaque assay (1-2 weeks) Plaque assay (1-2 weeks) Foci of infection Foci of infection Rapid titer (2 days) Rapid titer (2 days) Measure by immunostaining viral antigens Measure by immunostaining viral antigens Endpoint dilution Endpoint dilution TCID 50 TCID 50 FACS after immunostaining viral antigens FACS after immunostaining viral antigens
