ADVIA Concepts &Principles

Presented by:NARGIS KHOSO

14th Oct 2015

Overview -What is ADVIA2120i?

-Components of ADVIA2120i-Principle-Reagents and Loading reagents-Running samples-Quality Control-Maintenance

The ADVIA2120/2120i Hematology System is a fully automated diagnostic instrument with a throughput of 120 samples / hour(CBC/diff)The analyzer uses whole blood sample to provide the following types of result :

-Complete blood count(CBC)-CBC plus White cell differential count-Reticulocyte absolute, percent & indices count(retic)-CBC/diff plus retic(CBC/diff/retic)-CBC/retic

ADVIA2120/ADVIA2120i

ParametersCBC Results WBC, RBC, HB,* HCT, MCV, MCH, MCHC, CHCM, RDW, HDW, CH, CHDW, PLTDifferential Results (Absolute and %) NEUT, LYMPH, MONO, EOS, BASO, LUC(Large Unstained Cells)Platelet Results PLT, MPV, PDWReticulocyte Results Absolute and % RETIC,MCVr,CHCMr, RDWr,** HDWr,** CHr, CHDWr**CSF Assay Results (Optional)

ADVIA 2120i Hematology SystemTechnical Specifications

Morphology Results WBC Left Shift, Atypical Lymph, Blasts, Immature Granulocytes, Myeloperoxidase DeficiencyRBC and PLT NRBC, ANISO, MICRO, MACRO, HYPO, HYPER,RBC Fragments, RBC Ghosts, Platelet Clumps, Large Platelets

Test Selectivity/ThroughputCBC 120 samples/hrCBC/DIFF 120 samples/hr

CBC/RETIC 74 samples/hrRETIC 74 samples/hrCBC/DIFF/RETIC 74 samples/hr

Sample Modes/VolumesAutomatic Closed Tube 175 μLManual Closed Tube 175 μLManual Open Tube 175 μL

Data ManagementDatabase 10,000-patient storage capacity including graphics

Workstation SpecificationsComputer Windows 2000,® 40-gigabyte hard disk drive,256-MB memory, CD-RW, full-size keyboard

The ADVIA2021i Hematology System is made up of 2 major components :

A- The analyzer contains all the electronic, pneumatic, hydraulic & sampler mechanisms, as well as the on board reagent storage for all reagents: Defoamer, Wash solution except Sheath RINSE. The waste system is a sub component of analyzer.

COMPONENTS OF ADVIA2120i

1 Auto sampler2 Manual open-tube sampler3 Manual closed-tube sampler4 Unifluidics™ technology5 Perox optical assembly6 RBC optical assembly7 Hgb colorimeter assembly8 Sample, sheath, and diaphragm pumps9 Reagents, sheath, wash, and rinse10 Vacuum and pressure regulator knobs11 Touchpad12 Manual barcode reader13 Waste removal system

B. Personal Computer(PC) includes a monitor , mouse, keyboard & interconnect cabling

COMPONENTS OF ADVIA2120i

The auto sampler automatically transports, mixes, identifies & aspirates samples in closed tubes.The auto sampler is a rack-style sampler with a capacity of 150 sample tubes: 15 racks of 10 tubes. The individual racks are barcoded for rack and position numbers.

Auto sampler

In open tube aspiration, sample probe is immersed in to the specimen.Pressing the push to aspirate plate , starts the sampling

Manual open tube sampler

The capped vial is inserted upside down in to the centering collar

Close tube sampler

This block is made up of 8 plates which are pathway for fluid, air flow, valves & reaction chambers.Reaction Chambers are: -Hb reaction chamber -Baso reaction chamber -RBC reaction chamber -Retic reaction chamber -Perox reaction chamber

Unified Fluid Circuit(UFC)

Its made up of 2 ceramic discsThe rear disk is stationary .The front disc rotates to shear or divide sample into appropriate aliquots for analysis

Shear Valve

The perox optical assembly directs light from halogen lamp to the perox flow cell. The use of a sheath stream in the flow cell allows a cell-by-cell measurementof light scatter and absorption.

Perox optical assembly

The laser optical assembly uses a laser light source. This optical assembly is shared byRBC/Plt, retic, and baso/lobularity channels.The use of a sheath stream in the flow cell allows cell-by-cell measurement of low- andhigh-angle light scattering and absorption.

Laser Optical Assembly

The hemoglobin colorimeter assembly takes voltage readings that correspond to the amount of transmitted light that passes through the reaction chamber. The system uses the readings to derive the hemoglobin concentration.

Hemoglobin Colorimeter Assembly

The perox sample & sheath pumps are located on the left side of the analyzer.The RBC/baso/retic sample & sheath pumps are located on the right sideof the analyzer.

Sample, Sheath, and Diaphragm Pumps

The touchpad, located on the lower right of the analyzer, by which you operate the analyzer.On – Provides power to the analyzer assemblies and power supplies. After On is pressed, samples through the manual open- and closed-tube samplers can be processed within 2.5 minutes, while samples through the auto sampler can beginprocessing within four minutes.Standby – Sets the analyzer to a lower power state. To exit, press Standby.Start/Stop Sampler – Starts the auto sampler operation or stops the auto sampler.Eject Rack – Moves all racks within the auto sampler into the output queue. Rack in Sampler – Lights up when a sample rack is in the auto sampler,including the input queue.Off – Turns off the

Touch pad

The manual barcode reader is used to enter information from labels on sample tubes, reagent containers, controls, and calibrators.

Manual Barcode reader

All analyzer waste is stored in an 11-liter waste container. This container can be a stand-alonecontainer, which has to be emptiedmanually, or part of the automatic waste removal system. When the fluidlevel in either of the containers reachesthe maximum-level line (app. 8 liters), an error message appears on the Statusline on the personal computer monitor. The analyzer will not aspirate any more samples until the waste container is emptied.

Waste removal system

Principle

Laminar flow is induced by injecting a slowmoving sample dilution into a stream of fast-moving sheath fluid which prevents mixing. The sheath fluid surrounds the diluted sample and narrows it, so that cells flow through the sensing zone in single file…this process is known as hydrodynamic focusing.

Principle

CBC TIMEPACDIFF TIMEPACEZ WashPEROX SHEATHSHEATTH RINSEDEFOAMERAUTO RETIC

Reagents

Reagent Loading

Reagent Loading

To run patient samples from the manual open tube sampler:1.Run samples in the following order : a. Controls(control label) optional b. Patient samples(sample ID label)

2.Scan the tube label or enter the sample information in the manual sample ID tab

Running the Patient Sample

NOTE : Ensure the sample ID displays on the status line before aspirating a sample

4.Aspirate the samplea. Position tube so that probe is immersed into mixed well sample

b. Press the aspirate plate sample light flashes during aspiration ,when it stops flashing , remove tube

5.Validate results when available

Recommended controls are ADVIA TESTpoint Haematology Controls (Low, Normal and High) that should be keep and store at 2-5 degree C.*Run controls at the start of the patient workload, evaluate all controls before reporting patient results. Allow to reach the controls room temperature before use.*Mix controls properly before run.*Scan tube barcode by barcode reader*Sampler probe is immersed in to mix well sample only deep enough (Approximately 0.25 inch)*Press the aspirate plate.

Quality Control

Daily Maintenance*System wash Time 6.5 minAnalyser mode should be ready to run At utilities menu, select Hydrolytic functionSelect system wash, select 1 cycle and press StartNOTE: Automatic wash cycle has to be at least 3 cycles a day.

Maintenance

Clean the shear Valve and aspiration pathway in the UFC(Unified Fluid Circuit) by using 3% bleach and Distilled water (DW)

Weekly Maintenance

Replace both sheath filter1. PEROX filter (0.45 Um)2. Laser side Filter (0.22 Um) Clean air filter Clean 50Um plunger

Monthly Maintenance

Replace 1000Um plungerReplace 4 drain filter

6 months Maintenance

THANK YOU