Bacteriology 4th Practical

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PRESS F1 FOR GUIDEANCE. Bacteriology 4th Practical. MFSH 2003. Contents. Staphylococcus & Streptococcus Station 1: Staphylococcus VS. Streptococcus3 Staphylococcus (aureus & epidermidis)4 Station 2: Gram stain of Staphylococcus5 - PowerPoint PPT Presentation

Text of Bacteriology 4th Practical

  • MFSH 2003PRESS F1 FOR GUIDEANCE

  • Staphylococcus & StreptococcusStation 1: Staphylococcus VS. Streptococcus3Staphylococcus (aureus & epidermidis)4Station 2: Gram stain of Staphylococcus5Station 3: Gram stain of staphylococcus (pus)6Station 4: Staphylococcus growth on blood agar7Station 5: Deoxyribonulease test 8Station 6: Mannitol sugar fermentation test 9Station 7: Coagulase test 10Station 8: Antibiotic sensitivity test, treatment& diseases11Station 9: Summary and revision12Streptococcus13Station 10: Gram stain of Streptococcus 14Station 11: Gram stain of Streptococcus (pus)15Station 12: Gram stain of Streptococcus pneumoniae16Station 13: Gram Stain of Streptococcus pneumoniae17Station 14: Capsule stain of Streptococcus pneumoniae18Station 15: Alpha heamolytic Streptococci 19Station 16: Beta heamolytic Streptococci20Station 17: Gama or non-heamolytic Streptocooci21Station 18: Optochin test22Station 19: Bacitracin test23Station 20: Capsule of Streptococcus Pyogens24Station 21: Lancefield Grouping of beta heamolytic Streptococci 25Station 22: Treatment & diseases26Station 23: Summary and revision27

  • Staphylococcus StreptococcusSalineSalineH2O2 H2O2H2O + O2 (bubbles)H2O2Bring 2 slides2) Add saline3) Put some of the bacteria 4) Add H2O2 5) See the difference6) Take a look on the movie Staph Vs Strept included in the cdStation 1

  • Station 2

  • Station 3StaphylococciPus cells

  • Station 4 .

  • Station 5

  • Station 6MSA = Mannitol sugar agarColor not changedColor changed to yellow

  • Station 7Liquid state Coagulated state

  • Station 8Not all the members of the same species share all properties together. For example, not all staphylococcus aureus are sensitive to the same antibiotics. (some of them are resistant to penicillin and others are not).BacteriaControlWhen the distance between the control and the antibiotic is shorter than or equal to the distance between the antibiotic and the bacteria. A =< BThe bacteria is called sensitive to that anti-biotic When the distance between the control and the antibiotic is longer than the distance between the antibiotic and the bacteria. A > BThe bacteria is called resistant to that anti-biotic SensitiveSensitiveResistantSensitiveSome tribes of Staphylococcus areus are sensitive to Penicillin (also Cloxacilin, Methicillin) while others are not.Staph.aureus Infections:-1- Pyogenic infections and abscesses 2- Osteomylitis3- Food poisoning milk products vomiting4- Toxic shock syndrome in young women 5- SSSS Staphylococcus Scalded Skin Syndrome

    Treatment:-Drug of choice Cloxacilinif MRSA, (beta-lactamase producers) then we use VancomycinStaph.epidermidis & Staph.saprophyticusInfections:-Epidermidis endocarditis with patients with catheters, cannulae & other artificial objects. Saprophytics Urinary tract infections in young female

    Treatment:- Vancomycin

  • Station 9Gram stainColor on Blood agarMannitol fermentation testDNAse testCoagulase testCatalase +ve+ve YellowishWhitish+ve yellow -ve red +ve-ve +ve-ve +ve+ve

  • Station 10

  • Station 11

  • Station 12

  • Station 13

  • Capsule(transparent)Organism (red)Station 14

  • Streptococcus can be divided upon their hemolytic activity into 3 groups: Hemolytic (e.g. Streptococcus pneumoniae) Hemolytic (e.g. Streptococcus pyogenes) These are also grouped by the LACEFIELD grouping {from A-H, K-W} as you will see later 3) or non Hemolytic (e.g. Streptococcus mutans)Station 15

  • Station 16

  • Station 17

  • No colonies around the OPTOCHIN There are colonies around the OPTOCHIN Station 18

  • There will be some colonies around the BACITRACIN strip.Station 19Upon the Lancefield grouping

  • Station 20

  • Station 21

  • Station 22Alpha hemolytic streptococci (pnemococcus)Infection:-acute pnemoniasinusitisotitis mediameningitis

    Treatment:-Penecillin

    Vaccination:By the Polysaccharide capsuleBeta hemolytic streptococci gr.A (pyogens)Infection:-pharyngitis (sore throat)tonsilitisTreatment:-PenecillinSome notes:All Staph. :- Vancomycin But in non MRSA:- Cloxacilin is betterAll Strept :- Penecilin

  • Gram stainColor on Blood agarColor on ChocolateHemolytic activityLancefiel GroupingOptochin testBacitracin testCatalase +ve +ve +ve Green Clear No Chg. Green No Chg. No Chg. Alpha Beta Non +ve -ve -ve -ve +ve -ve -ve -ve -veStation 23 NO Gp.A NO

  • A word can change the outcome of many things. So, if you either liked or didnt like this project, why keep your thoughts to yourself ?Send us your comments and ideas to COORDINATOR1422@YAHOO.COM, It will mean too much to us, even if it was just one wordTHANXM. F. Shaheen 2003