BD FACS Canto II

The BD FACS Canto II offers three lasers and is able to detect up to 8 parameters ( fluorochromes) at the same time!

For the analysis at the Canto II you have the choice between two different configurations:

Configuration 4-2-2 (BD default)

A photodiode is used to detect the FSC signal!

Detector Array

Laser Parameter PMT- Position

BP-Filter

LP-Filter

Examples for possible

Fluorochromes Trigon 405 nm

-violett- 1 2

A B

510/50 450/50

502 LP

AmCyan Pacific Blue

Octagon 488 nm -blue-

3 4

5 6

SSC (Granularity)

A B C D E F G H

780/60 670 LP

585/42 530/30 488/10 Empty Empty

735 LP 655 LP 610 LP 556 LP 502 LP

PE-Cy7 PerCP, PerCP-Cy5.5

PE

FITC

Trigon 633 nm -red-

7

8

A B C

780/60 685 LP 660/20

735 LP APC-Cy7

APC

Configuration Copy of 4-2-2

A photodiode is used to detect the FSC signal!

The samples can only be injected via 5mL Round-Bottom Tubes with a maximum filling volume of 4mL !

Technical Highlights Lasers 488-nm solid state, 20-mW laser output

633-nm HeNe, 17-mW laser output 405-nm solid state diode, 30-mW fiber

Flow cell 180 x 430µm rectangular quartz flow cell FSC Detector Photodiode SSC Detector Photomultiplier with 488/10 BP Maximum sample acqusition Rate

10,000 events per second

Parameters Area (A), Width (W), Height (H) for all channels with up to two ratios and Time (T)

Compensation Full inter-beam matrix during or post acquisition

Software BD FACS DivaTM Software v.6.1.3

Detector Array

Laser Parameter PMT- Position

BP-Filter

LP-Filter

Examples for possible

Fluorochromes Trigon 405 nm

-violett- 1 2

A B

C

529/24 457/50

Empty

502 LP v-GFP, v-YFP CFP, Hoechst, BFP, Pacific

Blue, Cerulan

Octagon 488 nm -blue-

1 2

3 4 5

SSC (Granularity)

A B

C D E F G H

780/60 670 LP

529/24 509/20 488/10 Empty Empty

735 LP 610 LP

556 LP 525 LP 502 LP

PE-Cy5, PE-Cy7 PerCP, PerCP-Cy5.5, PI,

7AAD, PE-Texas Red, e-RFP

GFP, Venus, YFP FITC, eGFP, eYFP

Trigon 633 nm -red-

1

2

A B C

780/60

660/20

735 LP 685 LP

APC-Cy7

APC