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Bio-Reagents Oligo Synthesis Gene Synthesis Peptide Synthesis Protein Expression Antibody Production Life Science Products and Services Catalog 2012-2014

Bio-Reagents Oligo Synthesis Gene Synthesis Peptide ... Spnosors/biomatik_catalog_2… · A2115 ACrylAmidE Ultra Pure 100G C 3H 5NO Purity Conductivity (40%, Water) Melting Point

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Page 1: Bio-Reagents Oligo Synthesis Gene Synthesis Peptide ... Spnosors/biomatik_catalog_2… · A2115 ACrylAmidE Ultra Pure 100G C 3H 5NO Purity Conductivity (40%, Water) Melting Point

Bio-Reagents

Oligo Synthesis

Gene Synthesis

Peptide Synthesis

Protein Expression

Antibody Production

Life Science Products and Services

Catalog 2012-2014

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Since 2002, Biomatik has provided worldwide researchers with supe-rior quality and exceptional service. As a leading supplier of life sci-ence products and custom services, Biomatik offers industry-leading

innovation, quality and customer-satisfaction. Biomatik offers a broad range of bio-reagents, including biochemicals, enzymes, PCR related products, biokits, culture media, and catalog an-tibodies. Most of our products are in stock - in fact, over 98% of our orders are filled from stock, so you can expect the shortest turnaround time possible. Biomatik also provides a comprehensive portfolio of custom services that include custom oligo, gene, peptide, protein and antibody services. We work closely with our partners at the R&D and production levels to pro-vide complete customized solutions to help unveil the mysteries of life sciences. It is extremely important to us to help our customers save research costs while maintaining high quality standards; in doing so, our customers as well as our organization have benefited. This benefit has resulted in in-creased competitiveness, growth, and name recognition - the true reward of quality performance. Quality and customer satisfaction is at the core of our operations - we sincerely invite you to join our list of satisfied cus-tomers worldwide.

“Biomatik brings you the finest Life Science Products & Custom Services at highly competitive prices. We are convinced that the products and ser-vices we offer will meet and exceed your expectations.”

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1. Biochemicals & Enzymes ...............................................................3-40

2. PCR, qPCR & RT-PCR ...................................................................41-44

3. BioKits .........................................................................................45-48

4. Markers & Ladders ......................................................................49-58

5. Culture Media ..............................................................................59-64

6. Catalog Antibodies .......................................................................65-72

7. Custom Services ............................................................................... 73

7-1 Custom Oligo Synthesis .......................................................... 74

7-2 Custom Gene Synthesis .......................................................... 75

7-3 Custom Peptide Synthesis ....................................................... 76

7-4 Custom Protein Expression ..................................................... 78

7-5 Custom Antibody Production ................................................... 79

Appendix - Technical Reference Data .................................................81-92

Table of Contents

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Biomatik supplies life science products and custom ser-vices through its offices and authorized distributors. De-tails about our offices and distributors can be found at: www.biomatik.com.

For technical and sales inquiries:Phone: 1(800) 836-8089, 1(519) 489-7195Fax: 1(877) 221-3515, 1(519) 231-0140E-mail: [email protected]

Pricing and Payment Information Our prices are quoted in both US dollars and Cana-dian dollars. Applicable taxes, shipping charges and any special handling and packaging charges are ex-cluded and will be added to the invoice. Prices subject to change without notice. F.O.B Biomatik shipping point. Standard payment term: Net 30 days from day of in-voice. We also accept VISA, MasterCard and PayPal.

QuotationsWe would be pleased to provide you with a quote for: a) Product quantities which are different than catalog size;b) Custom made products, including custom synthetic genes, peptides, proteins and antibodies.

Conditions of SalesAll of Biomatik’s products are intended for laboratory research use only and not to be used for any other pur-pose, including but not limited to drug, food, food addi-tive, cosmetics or clinical use in humans. We reserve the right to insist on a written order and/or references be-fore proceeding on an order. The buyer may be asked to provide written assurance that chemicals not be pur-chased/resold for an improper use.

Return and Replacement PolicyFor catalog products, please contact our Customer Ser-vice for a return authorization number before returning any product. No returns will be accepted more than 30 days after shipping date. A restocking fee may be levied depending upon the nature of the return. We reserve the right not to accept the cancellation of orders, unless otherwise expressed in written form.

For custom made products, once an order is accepted by Biomatik, it cannot be terminated without the prior written consent of Biomatik. Custom made products are not returnable. Please visit our website for terms and conditions which are specific for different custom made products.

Warranties and LiabilitiesBiomatik warrants that each product conforms to its specification at the time of sale. Warranty is exclusive, and Biomatik makes no other warranty, express or im-plied, including any implied warranty of merchantability or fitness for any particular purpose. Biomatik shall not in any event be liable for any consequential, general, incidental, or contingent damages whatsoever of any kind. Biomatik’s sole and exclusive liability and buyer’s exclusive remedy with respect to products proved to Biomatik’s satisfaction to be defective or nonconforming shall be (a) replacement of such products or (b) refund of the purchase price actually paid therefore by Buyer, in Biomatik’s sole discretion, upon the return of such prod-ucts in accordance with Biomatik’s instructions.

TrademarksBiomatik® -Biomatik CorporationCoomassie® -Imperial Chemical Inc.Nonidet® -Shell International Petroleum CoSpinKleanTM -Biomatik CorporationTriton® -Union Carbide Chemicals and PlasticsTween® -ICI Americas Inc.

ORDERING INFORMATION

Biomatik Corporation9-140 McGovern Drive

Cambridge, Ontario, N3H4R7

Canada

Biomatik USA, LLC105-501 Silverside Rd

Wilmington, Delaware, 19809

USA

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1- Biochemicals & Enzymes

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S A2102 ACESWater soluble, low interference with biological processes and biological membranes

(penetration, solubilization, adsorption on surface), non-toxic and low UV absorption at

wavelength >260nm.

High PurityCAS [7365-82-4]

50G250G

C4H10N2O4SPuritypH (1%, Water) at 25°CpKa at 25°CA280 (5%, Water)

MW 182.20>99.0%3.6-4.4

6.58-6.98<0.02

A2103 ACEtiC ACid, GlACiAlMeets ACS specifications.

ReagentCAS [64-19-7]

100ML 500ML

1LCH3COOHPurityResidue after EvaporationAcetic Anhydride Chloride SulfateHeavy MetalsIron

MW 60.05>99.7%

<0.001%<0.1%<1ppm<1ppm

<0.5ppm<0.2ppm

UN 2789

A4150 ACEtONE ACS 500ML(CH3)2COPurityColor (APHA)MethanolWater

MW 58.08>99.5%

<10<0.05%

<0.5%

CAS [67-64-1]UN 1090

1L

A2104 ACridiNE OrANGEA fluorescent stain for nucleic acids in agarose and polyacrylamide gels.

Concentrations of 120μM will detect DNA at levels of 25-50ng per band. Also used to

localize nucleic acids in a variety of cell types.

High Purity[10127-02-3]

10G50G

C17H20ClN3.1/2 ZnCl2 MW 370.00A2115 ACrylAmidE Ultra Pure 100G

C3H5NOPurity Conductivity (40%, Water) Melting PointFree Acrylic AcidIronLeadMagnesiumMoisture (KF)pH (10%, 0.1M NaCl) at 25 °CInsolublesDNase, Protease & RNase

MW 71.08>99.9%

<5µmhos84-86°C

<0.001%<0.0001%<0.0001%<0.0001%

<0.2%5.5-6.5

<0.005%None Detected

CAS [79-06-1]UN 2074

500G2.5KG

5KG

A2118 ACrylAmidE Electrophoresis 100GC3H5NOPurityConductivity (40%, Water)

MW 71.08>99.5%

<5µmhos

CAS [79-06-1]UN 2074

500G2.5KG

5KG

A2119 ACtiNOmyCiN dFrom streptomyces species, antibiotic. Inhibits cell growth by complexing with DNA and interfering with RNA synthesis. At high dose of 2µg/ml, it inhibits the transcription of all RNA species. At a lower dose of 40ng/ml it inhibits the synthesis of rRNA. Used in cell culture as a selection agent. Soluble in DMSO and ethanol. Recommended working concentration: 1 µg/ml in DMSO. Prevents RNA transcription by binding DNA at transcription initiation sites.

Ultra PureCAS [50-76-0] Store: 4°C

5MG

C62H86N12O16

Purity (HPLC)Solubility (0.1%, DMSO) (P/F)

MW 1255.44>98.0%

Pass

Code Product Description Size

Biochemicals & Enzymes

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1- Biochemicals & Enzymes

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S A2102 ACESWater soluble, low interference with biological processes and biological membranes

(penetration, solubilization, adsorption on surface), non-toxic and low UV absorption at

wavelength >260nm.

High PurityCAS [7365-82-4]

50G250G

C4H10N2O4SPuritypH (1%, Water) at 25°CpKa at 25°CA280 (5%, Water)

MW 182.20>99.0%3.6-4.4

6.58-6.98<0.02

A2103 ACEtiC ACid, GlACiAlMeets ACS specifications.

ReagentCAS [64-19-7]

100ML 500ML

1LCH3COOHPurityResidue after EvaporationAcetic Anhydride Chloride SulfateHeavy MetalsIron

MW 60.05>99.7%

<0.001%<0.1%<1ppm<1ppm

<0.5ppm<0.2ppm

UN 2789

A4150 ACEtONE ACS 500ML(CH3)2COPurityColor (APHA)MethanolWater

MW 58.08>99.5%

<10<0.05%

<0.5%

CAS [67-64-1]UN 1090

1L

A2104 ACridiNE OrANGEA fluorescent stain for nucleic acids in agarose and polyacrylamide gels.

Concentrations of 120μM will detect DNA at levels of 25-50ng per band. Also used to

localize nucleic acids in a variety of cell types.

High Purity[10127-02-3]

10G50G

C17H20ClN3.1/2 ZnCl2 MW 370.00A2115 ACrylAmidE Ultra Pure 100G

C3H5NOPurity Conductivity (40%, Water) Melting PointFree Acrylic AcidIronLeadMagnesiumMoisture (KF)pH (10%, 0.1M NaCl) at 25 °CInsolublesDNase, Protease & RNase

MW 71.08>99.9%

<5µmhos84-86°C

<0.001%<0.0001%<0.0001%<0.0001%

<0.2%5.5-6.5

<0.005%None Detected

CAS [79-06-1]UN 2074

500G2.5KG

5KG

A2118 ACrylAmidE Electrophoresis 100GC3H5NOPurityConductivity (40%, Water)

MW 71.08>99.5%

<5µmhos

CAS [79-06-1]UN 2074

500G2.5KG

5KG

A2119 ACtiNOmyCiN dFrom streptomyces species, antibiotic. Inhibits cell growth by complexing with DNA and interfering with RNA synthesis. At high dose of 2µg/ml, it inhibits the transcription of all RNA species. At a lower dose of 40ng/ml it inhibits the synthesis of rRNA. Used in cell culture as a selection agent. Soluble in DMSO and ethanol. Recommended working concentration: 1 µg/ml in DMSO. Prevents RNA transcription by binding DNA at transcription initiation sites.

Ultra PureCAS [50-76-0] Store: 4°C

5MG

C62H86N12O16

Purity (HPLC)Solubility (0.1%, DMSO) (P/F)

MW 1255.44>98.0%

Pass

Code Product Description Size

Biochemicals & Enzymes

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A2121 AdENiNE, FrEE BASE (VitAmiN B4)A purine derivative with essential functions for all known life forms. Required for energy metabolism and as a component of DNA and RNA. Component of many culture media.

High PurityCAS [73-24-5]

25G100G250G

C5H5N5 PurityHeavy MetalsNitrogen ContentLoss on DryingResidue on Ignition Solubility (0.05%, Water) (P/F)

MW 135.14>98.0%

<0.001%50.2-53.4

1.0%0.1%Pass

A2122 AdENiNE SulFAtESynonym(s): Adenine hemisulfate salt, 6-Aminopurine sulfate Component of yeast dropout media including AHC Medium for selection of strains hosting YACs.

High PurityCAS [321-30-2]

5G25G

100G

(C5H5N5 )2.H 2SO4.2H2OPurity Solubility (0.05%, Water) (P/F)Water (KF)

MW 404.37>99.0%

Pass<10.0%

A2125 AdENOSiNE, FrEE BASESynonym(s): Adenine riboside, Adenine-9-β-D-ribofuranoside. A purine nucleoside essential for a variety of  metabolic processes including  energy transfer, signal transduction and mediation of inflammatory pathways.

High Purity CAS [58-61-7]Store: 4°C

5G25G

100G

C10H13N5O4 Purity Loss on Drying

MW 267.24>99.0%

<0.5%

A2123 AdENOSiNE-5’-dipHOSpHAtE [Adp], diSOdium diHydrAtEInvolved in energy and nucleic acid metabolism

Ultra Pure CAS [16178-48-6] Store: -20°C

500MG1G5GC10H13N5O10P2Na2 .2H2O

A250/A260A280/A260ATPHeavy MetalsArsenicSolubility (5%, Water) (P/F)

MW 507.170.74-0.820.13-0.19

<1.0%<0.002%

<0.0001%Pass

A2124 AdENOSiNE-5’-mONOpHOSpHAtE [Amp], diSOdium HEXAHydrAtE Intermediate in energy metabolism.

Ultra PureCAS [4578-31-8] Store: -20°C

1G10G25G

C10H12N5O7PNa2 .6H2OA250/A260A280/A260 Heavy MetalsSolubility (5%, Water) (P/F)

MW 499.190.79-0.900.18-0.22 <0.002%

Pass

100G

A2160 AdENOSiNE-5’-tripHOSpHAtE [Atp], diSOdium triHydrAtE An essential nucleotide triphosphate with a variety of roles in energy metabolism, signal transduction and nucleic acid metabolism. 

Ultra PureCAS [987-65-5] Store: -20°C

1G5G

25GC10H14O13N5P3Na2.3H2OPurityA250/A260A280/A260AMP & ADPSodium (Flame Photom) Heavy MetalsMoistureSolubility (5%, Water) (P/F)

MW 605.24>98.0%

0.75-0.810.14-0.18

<0.5%7.0-8.0

<0.003%<10.0%

Pass

100G

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Code Product Description Size

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A2127 AGArOSE t1A standard melting/gelling agarose. All purpose, high purity agarose, suitable for routine

nucleic acid analytical and preparative applications. Exceptional band resolution and

clarity.

Biotechnology CAS [9012-36-6]

25G50G

100G500G

2.5KGGel Strength (1.5%)

Gelling Range (1.5%)

Melting Range (1.5%)

EEO

Sulfate

Moisture

DNase & RNase

>1200g/cm2

36-39°C

87-89°C

<0.12

<0.15%

<10%

None Detected

A2132 AGArOSE t3Suitable for general electrophoresis applications. It provides good resolution and is cost

effective for high volume users.

Biotechnology CAS [9012-36-6]

25G50G

100G500G

2.5KG

Gel Strength (1.5%)

Gelling Range (1.5%)

Melting Range (1.5%)

EEO

Sulfate

Moisture

DNase & RNase

>1000g/cm2

36-39°C

87-89°C

<0.15

<0.15%

<10%

None Detected

A2128 AGArOSE t2High Gel Strength allowing for lower gel concentration. Suitable for Pulse Field Electrophoresis and the separation of high molecular weight DNA at lower concentrations. Exceptionally low absorption of staining agents.

Biotechnology CAS [9012-36-6]

25G50G

100G500G

Gel Strength (1.5%)Gelling Range (1.5%)Melting Range (1.5%)EEOSulfateDNase & RNase

>2200g/cm2

36-39°C87-89°C

<0.12 <0.12%

None Detected

A2129 AGArOSE t4, HiGH GEl StrENGtHAgarose T4 has highest Gel Strength and is suitable for Pulsed Field applications and high molecular weight DNA.

Biotechnology CAS [9012-36-6]

25G50G

100G500GGel Strength (1.5%)

Gelling Range Melting RangeEEODNase & RNase

>3200g/cm2

35.5-38.5°C86.5-88.5°C

<0.12 None Detected

A2131 AGArOSE t5, HiGH rESOlutiON, lOw mEltiNGA low melting agarose with super high resolution - resolve 238 bp & 242 bp bands. Unsurpassed clarity. DNA bands differing in size by 2% can be resolved in the range of 200 - 1,000 bp. This agarose is suitable for the analysis of AFLP’s (Amplified Fragment Length Polymorphisms), STR’s (Short Tandem Repeats) and tetranucleotide repeats. The low melting temperature of Agarose T5 makes it an excellent medium for analytical and preparative electrophoresis.

Biotechnology CAS [9012-36-6]

10G25G

100G

Gel Strength (1.5%)Gelling Temperature (1.5%)Melting Temperature (1.5%)EEOSulfate MoistureConductivity (1%, Water)DNase, RNase & Protease

>500g/cm2

30.0°C70.0°C <0.12

<0.11%<7.0%

<10µmhosNone Detected

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A2133 AGArOSE t6, HiGH rESOlutiON, HiGH StrENGtHA unique mixture of agarose formulated to provide high resolution of small nucleic

acids and PCR products. Ideal for small nucleic acids and PCR products. This product is

specially designed for analysis of DNA fragments less than 1,000 bp. Agarose T6 displays

precise banding patterns without smearing or high background fluorescence. The high

gel strength allows for easy-to-handle gels that are optimal for blotting applications.

Biotechnology CAS [9012-36-6]

10G25G

100G

Gel Strength (1.5%)Gelling Temperature (1.5%)Melting Temperature (1.5%)EEOSulfate DNase, RNase & Protease

>2000g/cm2

34-38°C85-89°C

<0.12 <0.10%

None Detected

A2134 AlBumiN, BOViNE SErum [BSA], FrACtiON VHeat shock isolated BSA suitable for use in a wide range of molecular biology applications, including Western Blotting, enzyme systems and as a protein supplement. Use as a blocking reagent for Southern, Northern, and Western Blotting applications. Also suitable as a nutrient in cell culture media.

BiotechnologyCAS [9048-46-8] Store: 4°C

10G25G50G

100G250G500GPurity

pH (2%, Water) at 25°CHeavy MetalsIronLoss on DryingDNase, RNase & Protease

>98.0%6.5-7.5

<0.001%<0.0005%

<5%None Detected

A2136 AlCiAN BluEUsed to stain glucosaminoglycans and other acidic polysaccharides in tissue samples. It can also be used as a bacterial stain.

High PurityCAS [33864-99-2]

5G25G

100GC56H68N16 S4Cl4CU

Dye ContentMW 1298.88Approx. 50%

A1130 AlKAliNE pHOSpHAtASE, 2-COmpONENt SyStEmAn enzyme commonly used for the removal of terminal phosphates from the 5’ ends of DNA or RNA strands to prevent re-annealing of cohesive ends and facilitate future radiolabeling of the strands. Is most often conjugated to a secondary antibody or cellular component (avidin or biotin). It includes 30U/μl of Alkaline Phosphatase in a 50% Glycerol solution and a dilution buffer that allows end user to prepare a 3U/μl solution for use in DNA modification.

BiotechnologyCAS [9001-78-9] Store: 4°C. Do not Freeze

1KU5KU

A2141 AmidO BlACKA stain for protein in paper chromatography. Used for staining protein after electrophoresis. Also a histological stain for hemoglobin.

High PurityCAS [1064-48-8]

5G25G

100GC22H14N6O9S2Na2

Dye ContentLambda Max

MW 616.50Approx. 80%615-621nm

A2142 AmmONium ACEtAtE Ultra Pure 500GC2H7N O2

Purity

Insolubles

pH (5%, Water) at 25°C

Chloride

Sulfate

Iron

Nitrate

Heavy Metals (as Pb)

MW 77.08

>97.0%

<0.01%

6.7-7.3

<0.0005%

<0.001%

<0.0005%

<0.001%

<0.0005%

CAS [631-61-8]Store: 4°C

1KG

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A4151 AmmONium CArBONAtEA mixture of variable proportions of Ammonium Bicarbonate and Ammonium Carbonate.

Ultra PureCAS [506-87-6]

500G1KG

(NH4)2 CO3

Assay (NH3)InsolublesSulfur CompoundsChlorideIronHeavy metals (as Pb)

MW 96.06>30%

<0.005%<0.002%

<0.0005%<0.0005%<0.0005%

A2143 AmmONium CHlOridE ACS 500GNH4ClPurityInsolubles pH (5%, Water) at 25°CSulfateCalciumIronMagnesiumPhosphateResidue on IgnitionHeavy Metals (as Pb)

MW 53.49>99.5%

<0.005%4.5-5.5

<0.002%<0.001%

<0.0002%<0.0005%<0.0002%

<0.01%<0.0005%

CAS [12125-02-9] 1KG

A2326 AmmONium CitrAtE, diBASiC (CitriC ACid, AmmONium SAlt, diBASiC)

High PurityCAS [3012-65-5]

500G1KG

(NH4)2HC6H5O7

PurityChlorideSulfateHeavy MetalsResidue after Ignition

MW 226.19>99.0%

<0.003%<0.01%

<0.003%<0.05%

A2146 AmmONium pErSulFAtE [ApS] ACS 25G(NH4)2S2 O8

PurityInsolublesIronManganeseChloride & ChlorateHeavy Metals (as Pb)Residue on IgnitionTitrable Free Acid

MW 228.19>98.0%

<0.005%<0.001%

<0.00005%<0.001%<0.005%

<0.05%<0.04meq/g

CAS [7727-54-0]UN 1444

100G500G

A2147 AmmONium pHOSpHAtE, diBASiC ACS 500G(NH4)2HPO4

PurityInsolublespH (5%, Water) at 25°CChlorideNitrateSulfateHeavy Metals (as Pb)IronPotassiumSodium

MW 132.06>98.0%

<0.005%7.7-8.1

<0.001%<0.003%

<0.01%<0.001%<0.001%<0.005%<0.005%

CAS [7783-28-0] 2.5KG

A2148 AmmONium pHOSpHAtE, mONOBASiC ACS 500GNH4H2PO4 Purity Insolubles pH (5%, Water) at 25°C Chloride Nitrate Sulfate Heavy MetalsIronPotassiumSodium

MW 115.03>98.0%

<0.005%3.8-4.4

<0.0005%<0.001%

<0.01%<0.0005%

<0.001%<0.005%<0.005%

CAS [7722-76-1] 2.5KG

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A2149 AmmONium SulFAtEMay be used for the precipitation or fractionation of proteins or for purification of antibodies. Useful for crystallographic analysis of nucleic acids and proteins.

Ultra PureCAS [7783-20-2]

500G2.5KG

5KG(NH4)2 SO4

PurityArsenicChlorideIronMagnesium Test (P/F)pH (5%, Water) at 25°CPhosphateSeleniumResidue on IgnitionHeavy Metals (as Pb)DNase, RNase & Protease

MW 132.14>99.5%

<0.0001%<0.0005%<0.0005%

Pass5.0-6.0

<0.0005%<0.002%<0.005%

<0.0002%None Detected

A2151 AmpHOtEriCiN BAn antifungal polyene believed to act by altering membrane permeability. A working concentration of 2.5µg/ml in DMSO is recommended.

USPCAS [1397-89-3] Store: -20°C

100MG500MG

C47H73NO17

Potency (Anhydrous) Amphotericin A Loss on Drying Residue after Ignition

MW 924.10>750µg/mg

<15.0%<5.0%<0.5%

A2154 AmpiCilliN, SOdium SAltDerivative of Penicillin. Inhibits cell-wall synthesis in gram- and gram+ bacteria. Used to select drug resistant plasmid bearing bacteria. Suggested stock solutions: 50mg/ml. Store stock solution at -20°C. Stable at 37°C for 3 days. Working concentration for E.Coli: 20-50µg/ml.

USPCAS [69-52-3]Store: 4°C

5G25G

100G

C16H18N3NaO4S Potency (anhydrous)pH (1%, Water) at 25°CMethylene ChlorideWater (KF)

MW 371.39845-988µg/mg

8.0-10.0<0.2%<2.0%

A2159 AprOtiNiNSource: Bovine lung. Highly purified by chromatography. A competitive and reversible serine protease inhibitor of chymotrypsin, trypsin, kallikrein and plasmin. Not an inhibitor of Factor Xa and thrombin. Soluble in water, 10mg/ml; also soluble in 70% methanol and 70% ethanol. Working concentration: 2-10µg/ml.

High PurityCAS [9087-70-1]Store: 4°C

100MG500MG

1G

C284H432N84O79S7

ActivityPurity (electrophoresis)

MW 6511.51>4500KIU/mg

One Single Band

A2203 BASiC FuCHSiN A stain for Gomori’s aldehyde-fuchsin method and periodic acid-Schiff method. Useful for staining bacterical flagella.

High PurityCAS [632-99-5]

5G25G

C20H20N3ClSolubility (2mg/ml Water) (P/F)Dye content

MW 337.86Pass

>80% A2205 BCip, tOluidiNE SAlt [BCip-t]

Chromogenic substrate for the detection of alkaline phosphatase. Often used with INT or NBT to amplify the sensitivity of staining. Insoluble in water. Solubility in DMF: 20mg/ml. 1mg/ml in 0.1M phosphate buffer gives a haze free solution.

Ultra PureCAS [6578-06-9] Store: -20°C

100MG250MG

1G

C8H6BrClNO4 P.C7H9NPurity (HPLC)Moisture

MW 433.62>99.0%

<2.0%

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A2207 BENZOiC ACid ACS 100GC6H5COOH PurityInsolubles (methanol)Heave Metals (as Pb)Residue on Ignition

MW 122.12>99.5%

<0.005%<0.0005%

<0.005%

CAS [65-85-0] 500G2.5KG

A2406 d-BiOtiN (VitAmiN H)A small biomolecule, 244 Daltons, that has high binding affinity to avidin and streptavidin often used to label antibodies and enzymes. Several molecules of biotin can react with a single protein molecule to increase the sensitivity at the site of detection in ELISA, immunohistochemistry and Western Blotting. Suitable for tissue culture. Used as a growth factor in mammalian cell culture. Used in affinity chromatography. Slightly soluble in water 0.2mg/ml, soluble in acids and bases. Insoluble in ethanol.

ReagentCAS [58-85-5]Store: 4°C

100MG500MG

1G5G

C10H16N2O3S Purity (RG) Heavy Metals (as Pb)

MW 244.31>99.0%

<0.001%

A2214 BiS-ACrylAmidE Ultra Pure 50GC7H10O2N2

PurityA290 (1%, Water)Conductivity (2%,water)Acrylic acid

MW 154.17>99.0%

<0.2<5µmhos <0.001%

CAS [110-26-9] 250G

A2215 BiS-triSImportant buffer for protein and nucleic acid systems.

Ultra PureCAS [6976-37-0]

25G50G

C8H19NO5

PuritypKa at 25°CpH (1%, Water) at 25°CSolubility (1.0%, Water)DNase, RNase & Protease

MW 209.20>99.0%

6.45-6.658.8-9.6

PassNone Detected

250G1KG

A4166 BlAStiCidiN S, HydrOCHlOridEBlasticidin, from Streptomyces griseochromogenes, is an antibiotic commonly used in gene selection. Blasticidin is active against both propkaryotic and eukaryotic. Resistance to blasticidin is conferred by the blasticidin resistence gene from bacillus cereus (bsr). Bsr codes for blasticidin deaminase. Solubility: Blasticidin.HCl is soluble in water, dilute acetic acid. Insoluble in organic solvents.Normally, bacteria are sensitive to blasticidin concentrations of 25-100µg/ml. Mammalian cells demostrate sensitivity at concentrations of 1-10µg/ml.

High PurityCAS [3513-03-9]UN 2811

25MG100MG

C17H26N8O5.HClPurity

MW 458.90>98%

A2217 BOriC ACid ACS 500GH3BO3 Purity Heavy Metals (as Pb) Insolubles (Methanol)ChloridePhosphateSulfateCalciumIron

MW 61.83>99.5%

<0.001%<0.005%<0.001%<0.001%

<0.01%<0.005%<0.001%

CAS [10043-35-3] 1KG5KG

A4137 BOriC ACid Biotechnology 500GH3BO3

Purity Heavy Metals (as Pb) Insolubles (Methanol)ChloridePhosphateSulfateCalciumIron DNase, RNase & Protease

MW 61.83>99.5%

<0.001%<0.005%<0.001%<0.001%

<0.01%<0.005%<0.001%

None Detected

CAS [10043-35-3] 1KG5KG

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A2218 BrAdFOrd rEAGENtReady-to-use, 1X concentrate. Bradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10 to 100µg/ml simply by increasing the volume of the dye generating a standard curve in the 10 to 100μg/ml range.

Biotechnology Store: 4°CUN 1805

100ML500ML

A2219 BriJ-35Polyoxyethylene Lauryl Ether.Non-ionic - containing no charge (polyoxyethylenes or alkyl glucosides). Non-ionic detergents are suited for solubilizing membrane proteins without altering biological activity. These non-denaturing, gentler detergents are ideal for studying the conformation and function of proteins because they keep secondary and tertiary structure intact. Non-ionic detergents efficiently separate hydrophilic proteins from membrane spanning, hydrophobic proteins.

High PurityCAS [9002-92-0]

250G500G

(C2H4O)nC12H25OHHydroxyl NumberMoisture (KF)

MW 1199.5740-60

<3.0%A4154 BrilliANt GrEEN

A stain for differentiation of E.coli from S.typhosa. Also used in bacteriological media.

CertifiableCAS [633-03-4]

10G25G

100GC27H34N2O4SDye Content

MW 482.69>85.0%

A2220 BrOmOCrESOl GrEEN, FrEE ACidA tracking dye for RNA gels. pH indicator, range 3.8 (yellow) to 5.4 (blue).

ACS CAS [76-60-8]

5G25G

C21H14Br4O5S MW 698.02

A2221 BrOmOCrESOl GrEEN, SOdium SAltA tracking dye for RNA gels. pH indicator, range 3.8 (yellow) to 5.4 (blue).

ACS CAS [62625-32-5]

5G25G

C21H13Br4O5SNa MW 720.02

A2222 BrOmOpHENOl BluEA tracking dye for nucleic acid and protein gels. pH indicator, range 3.0 (yellow) to 4.6 (blue).

ACS CAS [115-39-9]

5G25G

100GC19H10Br4O5S MW 669.96

A2223 BrOmOpHENOl BluE, SOdium SAltA tracking dye for nucleic acid and protein gels. pH indicator, range 3.0 (yellow) to 4.6 (blue).

ACSCAS [62625-28-9]

5G25G

100GC19H9Br4O5SNa MW 691.94

A2304 CACOdyliC ACid Ultra Pure 5GC2H7AsO2

PurityMW 138.00

>99.0%CAS [75-60-5]UN 1572

25G100G

A2303 CACOdyliC ACid, SOdium SAlt, triHydrAtE Ultra Pure 10GC2H6AsO2Na.3H2O Purity pH (5%, Water)

MW 213.91>98.5%8.0-9.0

CAS [6131-99-3]UN 1688

25G100G

A4155 CAFFEiNE USP 100GC8H10N4O2

Purity Heavy MetalsLoss on DryingResidue on Ignition

MW 194.1998.5-101.0%

<0.001%<0.5%<0.1%

CAS [58-08-2] 500G

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A2305 CAlCium CArBONAtE, ANHydrOuS Reagent 100GCaCO3 Purity ArsenicFluorideLeadLoss on DryingMagnesium and Alkali SaltsHeavy Metals

MW 100.09>98.0%

<0.0003%<0.005%<0.001%

<2.0% <1.0%

<0.003%

CAS [471-34-1] 500G

A2306 CAlCium CHlOridE, diHydrAtE ACS 500GCaCl2.2H2OAssaypH (5%, Water) at 25°CSulfateAmmoniumBariumIronMagnesiumOxidizers (as NO3)PotassiumSodiumStrontiumHeavy Metals (as Pb)Insolubles

MW 147.0299.0-105.0 %

4.5-8.5<0.01%

<0.005%<0.005%<0.001%<0.005%<0.003%

<0.01%<0.02%

<0.1%<0.0005%

<0.01%

CAS [10035-04-8] 1KG

A4156 CAlCium HydrOXidE USP 100GCa(OH)2

PurityAcid InsolublesHeavy MetalsMagnesium & Alkali Salts

MW 74.0995.0-100.5%

<0.5%<0.002%

<4.8%

CAS [1305-62-0] 500G

A2308 CApS High Purity 50GC9H19NO3SPuritypKa at 20°C

MW 221.32>99.0%

10.3-10.5

CAS [1135-40-6] 250G1KG

A2311 CArBENiCilliN, diSOdium SAltSemi-synthetic antibiotic used for gene expression research utilizing carbenicillin-resistant plasmids. Soluble in water, 50mg/ml.

Ultra PureCAS [4800-94-6]Store: 4°C

250MG1G5G

10GC17H16N2O6SNa2

Potency (anhydrous)WaterpH

MW 422.36>800μg/mg

<6.0%<6.0-7.5

A2313 CESium CHlOridESuitable for plasmid DNA, phage or cellular RNA preparation using density gradient centrifugation.

BiotechnologyCAS [7647-17-8]

25G100G500G

CsClPurityA260 (50%, Water)LeadSodiumRubidiumPotassiumLithiumMagnesiumNickelDNase, RNase & Protease

MW 168.36>99.9%

<0.1<0.0001%

<0.1%<0.1%

<0.05%<0.005%

<0.0001%<0.0001%

None Detected

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A4185 CESium CHlOridESuitable for plasmid DNA, phage or cellular RNA preparation using density gradient centrifugation.

Ultra PureCAS [7647-17-8]

25G100G500G

CsClPurityA260 (50%, Water)LeadIronCopperChromiumSodiumRubidiumPotassiumLithiumMagnesiumNickel

MW 168.36>99.9%

<0.05<0.0001%<0.0001%<0.0001%<0.0001%

<0.03%<0.03%

<0.001%<0.001%

<0.0001%<0.0001%

A2314 CESium SulFAtE Biotechnology 10GCs2SO4

PurityA260 (50%, Water)SodiumIronHeavy Metals (as Pb)MagnesiumPotassium

MW 361.89>99.5%

<0.1<0.2%

<0.002%<0.002%<0.005%

<0.05%

CAS [10294-54-9] 50G100G

A2315 CEtyldimEtHylEtHyl AmmONium BrOmidE [CdAB]Cationic - positively-charged (quaternary ammonium compounds). Utilized for applications ranging from solubilizing membrane proteins in non-denaturing conditions to creating denaturing conditions during gel electrophoresis.

High PurityCAS [124-03-8]UN 3077

50G100G500G

C20H44NBrPurity

MW 378.49>99.0%

A2317 CEtyltrimEtHyl AmmONium BrOmidE [CtAB]Cationic - positively-charged (quaternary ammonium compounds). Utilized for applications ranging from solubilizing membrane proteins in non-denaturing conditions to creating denaturing conditions during gel electrophoresis.

High PurityCAS [57-09-0]UN 3077

100G500G

C19H42NBr Purity

MW 364.45>99.0%

A2318 CHApSCHAPS is a zwitterionic detergent that combines the features of bile salts and N-alkyl sulfobetaines. CHAPS can be easily removed from gels or protein solutions by dialysis across a membrane.

Ultra PureCAS [75621-03-3]

1G5G

10G50G

100GC32H58N2SO7

PuritypH (10%, Water) at 25°CConductivity (10%, Water)Residue on IgnitionDNase, RNase & Protease

MW 614.89>99.0%5.0-7.0

<50µmhos<0.1%

None Detected

A2323 CHlOrAmpHENiCOlA broad-spectrum bacteriostatic that inhibits protein synthesis in gram+, gram- and anaerobic bacteria. Recommended working concentration: 20µg/ml.

USPCAS [56-75-7]

5G25G

100G

C11H12Cl2N2O5 PuritypH (2.5%, Water) at 25°C

MW 323.1397.0-103.0%

4.5-7.5A4119 CHlOrOFOrm

Chloroform is commonly mixed with equal volumes of phenol to enhance the extraction of DNA. In this extraction procedure, a biphasic mixture if formed. Chloroform is used to recover the aqueous phase overlaid with mineral oil in PCR procedures.

BiotechnologyCAS [67-66-3]UN 1888

1L

CHCl3PurityColor (APHA)Residue after EvaporationLead

MW 119.38>99.8% <10.0%

<0.001%<0.05ppm

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A2325 CHymOStAtiNA reversible serine protease inhibitor of a, b, g, d-chymotrypsin. Also inhibits papain and many other cysteine proteases. Working range: 10-100µM. Soluble in DMSO and Acetic Acid. May store stock solution at -20°C for over 3 months.

Ultra PureCAS [9076-44-2] Store: -20°C

5MG25MG

A4127 AlpHA-CHymOtrypSiNFrom Bovine Pancrease.

High PurityCAS [9004-07-3]

500MG1G

Activity (Dry Basis)Loss on DryingResidue on IgnitionTrypsin

>1,000U/mg<5.0%<2.5%<1.0%

Store: -20°C 5G

A2327 CitriC ACid ACS 500GC6H8O7 PurityInsolublesChloridePhosphate Sulfur Compounds (as SO4)IronLead

MW 192.13>99.5%

<0.005%<0.001%<0.001%<0.002%

<0.0003%<0.0002%

CAS [77-92-9] 2.5KG

A2328 CitriC ACid, triSOdium diHydrAtE (SOdium CitrAtE) A biological buffer used for cell culture, in vitro, enzyme assays and some electrophoretic applications at physiological pH.

ReagentCAS [6132-04-3]

500G2.5KG

C6H5Na3O7.2H2OPurityInsolublesHeavy Metals (as Pb)DNase, RNase & Protease

MW 294.10>99.5%

<0.001%<10ppm

None DetectedA2329 COOmASSiE® BrilliANt BluE G-250

Commonly used stain for the detection of protein bands following electrophoresis. Ultra PureCAS [6104-58-1]

5G25G

100GC47 H48N3O7S2Na MW 854.04 A2330 COOmASSiE® BrilliANt BluE r-250

Commonly used stain for the detection of protein bands following electrophoresis. Ultra PureCAS [6104-59-2]

5G25G

100GC45 H44N3O7S2NaDye ContentLoss on Drying

MW 825.99>65.0%

<5.0%A2331 CrEAtiNE pHOSpHAtE, diSOdium SAlt, tEtrAHydrAtE High Purity 1G

C4H 8N3O5 PNa2×4H2OPurity (Enzymatic)Water (KF)Inorganic PhosphateATP

MW 327.14>97.0%20-24%<0.5%

<0.002%

CAS [922-32-7]Store: -20°C

5G25G

A2332 CrESOl rEd, FrEE ACidA pH indicator (Orange to Yellow; range 0.2 - 1.8 and Yellow to Violet; range 7.0 - 8.8). Also used as a constituent of PCR reagent systems.

AnalyticalCAS [1733-12-6]

5G25G

C21H 18O5S Loss on DryingResidue after Ignition

MW 382.43<5.0%<0.1%

A2333 CrESOl rEd, SOdium SAltA pH indicator (Orange to Yellow; range 0.2 - 1.8 and Yellow to Violet; range 7.0 - 8.8). Also used as a constituent of PCR reagent systems.

AnalyticalCAS [62625-29-0]

5G25G

C21H 17O5SNa MW 404.40A2334 CryStAl ViOlEt

A component of Gram stain. A pH indicator (Yellow to Violet/Blue; range 0.0 - 1.8). Soluble in water and Ethanol.

USPCAS [548-62-9]

10G25G

100GC25H 30N3ClDye ContentInsolubles (Alcohol)WaterArsenicZincLeadResidue on Ignition

MW 407.9996.0-100.5%

<1.0%<7.5%

<0.001%<0.05%

<0.003%<1.5%

500G

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A2337 CupriC SulFAtE, pENtAHydrAtE ACS 100GCuSO4.5H2OPurityInsolubles CalciumChlorideIronNickelPotassiumSodiumNitrogen Compounds (as N)

MW 249.6898.0-102.0%

<0.005%<0.005%<0.001%<0.003%<0.005%

<0.01%<0.02%<0.002

CAS [7758-99-8] 500G

A4167 CyClOHEXimidE (ACtidiONE, NArAmyCiN A)Cycloheximide is an antibiotic produced by Streptomyces griseus, and acts as an inhibitor of protein synthesis in eukaryotes but not prokaryotes. Cycloheximide’s selectivity makes it active against yeast and fungi but tolerated by most bacteria. Cycloheximide is also used to induce apoptosis in HL-60 cells, T cell hybridomas, Burkitt’s lymphoma cells, and a variety of other cell types. Additionally, plant researchers have shown it to stimulate ethylene production in fruit and leaves. In molecular biology applications, it is used in the selection of wild type CAN1, CYH2 and LYS2 genes. Soluble in Chloroform, Ethanol and Methanol.

BiotechnologyCAS [66-81-9]Store: 4°CUN 2811

100MG1G5G

25G

C15H23NO4

Purity (TLC)PotencyWater Content (KF)Solubility

281.35>95.0%

>950μg/mg<1.0%

Clear/Colorless

A4192 CytOCHrOmE C Biotechnology 100MGCytochrome C is a hemoprotein. Cytochrome C is found in animal cells in the mitochondrial protein-lipid complex. Cytochrome C is extracted from porcine or bovine heart, and further purified by chromatographic method to remove contaminating proteases.

CAS [9007-43-6]Store: -20°C

1G

C42H54FeN8O6S2

PurityAppearanceIronBacterial Endotoxin

MW 886.90>95.0%

Carmine Lyophilized<0.60%

<5EU/mgA2404 dANSyl CHlOridE

A flourescent reagent used for labeling proteins and amino acids and for staining basic proteins.

Ultra PureCAS [605-65-2] Store: -20°C

1G5G

25GC12H12ClNO2SPurity

MW 269.75>99.0%

UN 3096

A2409 dENHArdt’S SOlutiON, 100X CONCENtrAtESuitable for molecular biology applications. A blocking reagent for Northern or Southern blots to prevent probe binding on open membrane space and non-target nucleic acids.

BiotechnologyStore: -20°C

10ML50ML

pH at 25°C 5.0-7.0

A2412 dEOXyCHOliC ACid, SOdium SAltAnionic - negatively-charged (carboxylates, sulfates or sulfonates in alkali metal form). Utilized for applications ranging from solubilizing membrane proteins in non-denaturing conditions to creating denaturing conditions during gel electrophoresis. Used for complete disruption of membranes.

High PurityCAS [302-95-4]

25G100G500G

C24H 39O4Na Purity (Dry Basis) Sodium Cholate Arsenic Heavy Metals

MW 414.56>99.0%

<2.0%<0.001%<0.005%

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A1105 dNA pOlymErASE i, lArGE FrAGmENt5 U/µl, supplied with 10x Reaction Buffer. DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3’ to 5’ exonuclease activity, but has lost 5’ to 3’ exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5’ termini. Unit Definition: One unit is the amount of enzyme required to convert 10 nmole of dNTPs to an acid-insoluble form in 30 minutes at 37°C.

BiotechnologyCAS [9003-98-9]Store: -20°C

200U1KU

A2442 dNASE i (dEOXyriBONuClEASE i)A dialyzed lyophilized powder from Bovine Pancreas. Purified by chromatography. Deoxyribonuclease I (DNase I) efficiently hydrolyzes single-stranded or double-stranded DNA in the presence of divalent cations, producing 3’- hydroxyl oligonucleotides. Useful in crucial RNA preparations from tissue or bacterial cell cultures.

BiotechnologyCAS [9003-98-9]Store: -20°C

100MG500MG

1G5G

ActivityLoss on DryingResidue on Ignition

>500U/mg<5.0%<1.0%

A4199 dNASE i (dEOXyriBONuClEASE i), rNAE FrEERNase free. A dialyzed lyophilized powder from Bovine Pancreas. Purified by chromatography.

Ultra PureCAS [9003-98-9]Store: -20°C

50KU100KU

ActivityRNase

>1800U/mgNone Detected

A2417 dEXtrAN SulFAtE (dEXtrAN SOdium SulFAtE)Made from MW 500,000 Dextran. Used for the detection of DNA or RNA in nucleic acid hybridization procedures. It effectively “excludes” probe from solution, accelerating the rate of annealing.

Ultra PureCAS [9011-18-1]

25G100G500G

PuritypH (1%, Water) at 25°CIronHeavy MetalsDNase, RNase & Protease

>98.0%6.4-8.0

<0.001%<0.005%

None DetectedA2403 dAB tEtrAHydrOCHlOridE, HydrAtE

Commonly used for detection of horseradish peroxidase in membrane-bound and In Situ applications. Insoluble end product is resistant to alcohol and fading, making it ideal for immunohistochemistry.

Reagent

CAS [868272-85-9]

Store: -20°C

1G

5G

C12H14N4 .4HCl.XH2OPurity

MW 360.11>98.0%

A2423 diEtHANOlAmiNE Reagent 100GHN(C2H4OH) 2

Purity (Dry Basis)Refractive IndexSpecific Gravity

105.14>98.50

>1.453%>1.092%

CAS [111-42-2]

UN 3082

500G

A2416 diEtHylpyrOCArBONAtE [dEpC]Inactivates RNase enzymes from water and laboratory supplies. Protects RNA samples against degradation. DEPC convalently modifies histidine residues in RNase enzymes. DEPC can be used with PBS or MOPS buffer, but is incompatible with TRIS and HEPES. Density: 1.12g/ml. To treat water with DEPC, add 1ml of DEPC to 1 Liter of deionized water (0.1%). Stir overnight, Autoclave to remove DEPC. Caution: CO2

pressure may build up in the package as a result of hydrolysis of the product.

High Purity

CAS [1609-47-8]

Store: 4°C

25ML

100ML

C6H10O5

Purity

MW 162.14

>97.5%A2425 N’,N-dimEtHylFOrmAmidE [dmF]

Meets ACS Specifications. Density: 0.94g/ml. ReagentCAS [68-12-2]

500ML

C3H7NOPurity

MW 73.09 >99.5%

UN 2265

A2424 dimEtHyl SulFOXidE [dmSO]Suitable for molecular biology experiments. Density: 1.10g/ml.

ACSCAS [67-68-5]

100ML500ML

C2H6SOPurity Residue after Evaporation Water (KF) Titratable Free Acid

MW 78.13 >99.9%<0.01%

<0.1%<0.001meq/g

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2014

BiOCHEmiCAlS & ENZymES

A2452 dl-ditHiOtHrEitOl [dtt]An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM). Also named as Dithiothreitol, or Cleland’s Reagent. CAS# is also identified as [27565-41-9].

BiotechnologyCAS [3483-12-3] Store: -20°C

1G5G

25G100G

C4H10O2 S2

Purity (-SH Content)A283 (0.02M, Water)Melting PointLoss on DryingDNase, RNase & Protease

MW 154.25>99.0%

<0.0539-43°C<0.50%

None Detected

A2501 E-64 Trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butaneIrriversable protease inhibitor of several cysteine proteases like papain,calpain, and cathepsin B, H, L and S. Solubility/Stability: 1-10mM stock solutions in DMSO or 50% ethanol are stable for several days at 4°C and several months at -20°C. 1mM aqueous solutions are stable for several months at -20°C. E-64 is stable in the presence of reducing agents.

BiotechnologyCAS [66701-25-5] Store: -20°C

5MG25MG

C15H27N5O5

PurityTLC

MW 357.41>98.0%

One Spot

A2503 EdtA, diSOdium, diHydrAtE Ethylenediaminetetraacetic Acid, Disodium, Dihydrate. A reversible metalloprotease inhibitor useful for chelating metal ions.

BiotechnologyCAS [6381-92-6]

500G2.5KG

C10H14N2O8Na2.2H2OPurityInsolublespH (5%, Water) at 25°CHeavy Metals (as Pb)IronDNase, RNase & Protease

MW 372.24>99.0%

<0.005%4.0-6.0

<0.005%<0.01%

None Detected

A2504 EdtA, FrEE ACidEthylenediaminetetraacetic Acid.

Ultra PureCAS [60-00-4]

500G2.5KG

C10O16N2O8

PuritypH (5%, Water) at 25°CHeavy Metals (as Pb)IronResidue on IgnitionMagnesiumDNase, RNase & Protease

MW 292.25>99.5%2.5-3.5

<0.001%<0.005%

<0.2%<0.0005%

None Detected

A2506 EGtAEthyleneglycol bis(2-aminoethyl ether)-N,N,N’,N’ tetraacetic acid.A calcium specific chelator. Because of its low affinity for Mg+, EGTA inhibits metalloproteases. Soluble in water, more soluble as pH increases. Typical solution: 100mM EGTA in 100mM NaOH.

Ultra PureCAS [67-42-5]

10G25G

100G

C14H24N2O10

PurityMelting PointHeavy Metals (as Pb)Loss on DryingDNase, RNase & Protease

MW 380.35>97.0%>238 °C

<0.001%<1.0%

None Detected

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A4126 ElAStASEFrom Porcine Pancreas. Activity >30 units/mg. For tissue dissociation and cell isolation applications. Purified by affinity chromatography to remove trace contaminating proteases. Unstable at < pH 3.5. Unit Definition: one unit corresponds to the amount of enzyme which liberates 1umol 4-nitroaniline per minute at 25°C and pH 7.8.

High PurityCAS [39445-21-1]Store: 4°C

100MG500MG

1G5G

A2507 EOSiN y, SOdium SAltCounterstain to hematoxylin. Used for staining exfoliative cytology.

CertifiableCAS [17372-87-1]

10G25G

100GC20H6O5Br4Na2

Dye ContentLoss on Drying

MW 691.86>88.0%

<8.0%

A2508 ErytHrOmyCiN A broad spectrum antibiotic that inhibits protein synthesis in both gram+ and gram- bacteria. Recommended working concentration: 100µg/ml.

USPCAS [114-07-8]

10G25G

100GC37H67NO13

PurityWater (KF)Residue on IgnitionThiocyanate

MW 733.9485.0-100.5%

<10.0%<0.2%<0.3%

A2509 EtHANOl, ANHydrOuS, dENAturEdDenatured with 15% Methanol.

BiotechnologyCAS [64-17-5]

500ML1L

C2H5OHPurity Methanol (by Volume)Water (by Volume)Color (APHA)

MW 46.07>85.0%<15.0%<0.14%

<5

UN 1170

A5110 EASyStAiNtm i Equivalent to SYBR® Green I. The nucleic acid gel stain is the most sensitive stain available for detecting nucleic acids in agarose and polyacrylamide gels. Less than 20 pg of double-stranded DNA can be detected in a single band of an EasyStain I-stained gel using 254nm epi-illumination, black and white Polaroid® 667 print film and an EasyStain photographic filter.

BiotechnologyCAS [163795-75-3]Store: -20°C

0.1ML

A2512 EtHidium BrOmidE [EB]Suitable for electrophoresis. At 0.5μg/ml, it is useful in agarose or acrylamide gels for band detection of as little as 10ng of nucleic acid at 312nm.

BiotechnologyCAS [1239-45-8]UN 2811

5G

C21H20BrN3

PurityLoss on Drying DNase, RNase & Protease

MW 394.32>98.0%

<5.0%None Detected

A2510 EtHidium BrOmidE [EB], 10mG/ml Suitable for electrophoresis. DNase & RNase: none detected.

Biotechnology 5ML10ML

A2604 FErriC CHlOridE, HEXAHydrAtE ACS 250GFeCl3.6H2OPurityInsolublesSulfateCopperFerrous Iron MagnesiumNitratePotassiumSodiumZinc

MW 270.3097.0-102.0%

<0.01%<0.01%

<0.003%<0.002%<0.005%

<0.01%<0.005%

<0.05%<0.003%

CAS [10025-77-1]Store: 4°CUN 3260

1KG

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A2607 FErrOuS SulFAtE, HEptAHydrAtE ACS 250GFeSO4.7H2O PurityInsolubles CalciumChlorideCopperFerric IronMagnesiumManganesePhosphatePotassiumSodiumZinc

MW 278.01>99.0% <0.01%

<0.005%<0.001%<0.005%

<0.1%<0.002%

<0.05%<0.001%<0.002%

<0.02%<0.005%

CAS [7782-63-0] 1KG

A2611 FOrmAldEHydE, 37% SOlutiONSuitable for use in Formaldehyde-Agarose Gel Electrophoresis of RNA. Density: 1.08g/ml

ReagentCAS [50-00-0]UN 1198

500ML1L

CH2OFormaldehydeMethanolColor (APHA)Acidity

MW 30.0336.5-38.0%10.5-13.0%

<10<0.03%

A2612 FOrmAmidE, dEiONiZEdFormamide lowers the melting temperature of nucleic acid duplexes and is widely used in applications such as Northern blotting, nuclease protection assays, S1 mapping and in situ hybridization. Biomatik’s formamide has been deionized and is RNase-free. Ideal for denaturing nucleic acids in hybridization and DNA sequencing procedures.

Ultra PureCAS [75-12-7] Store: 4°C

100ML500ML

CH3NOPurity Conductivity A280 CopperIronLeadZinc DNase, RNase & Protease

MW 45.04>99 .5%

<100µmhos <0.05

<0.00001%<0.0005%

<0.00005%<0.00005%

None DetectedA2419 d-FruCtOSE

Sugar supplement to media for specialized application.High PurityCAS [57-48-7]

500G2.5KG

C6H12O6

PurityLoss on DryingResidue on IgnitionChlorideHeavy Metals (as Pb)SulfateCalcium & MagnesiumArsenic

MW 180.16>99.0%

<0.5%<0.5%

<0.018%<0.0005%

<0.025%<0.005%

<0.0001%A2702 G418 SulFAtE

An aminoglycoside related to gentamycin. Used as a selection agent in bacterial genetics. Suitable for cell culture. Soluble in water and most aqueous buffers. Suggested working concentrations are 100μg-800μg/ml. A concentration of ~400μg/ml is needed for selection and ~200μg/ml for maintenance in mammalian cells. Stability: powder is stable for over 2 years at room temperature; solution is stable for at least 3 months if kept at 4°C. Effective concentrations: 100μg/ml-5mg/ml.

High PurityCAS [108321-42-2]Store: 4°C

100MG500MG

1G5G

C20H40N4O10.2H2SO4

PotencypHLoss on Drying

MW 692.70>700μg/mg

5.0-6.0<10.0%

A2401 d (+) GAlACtOSESugar supplement to media for specialized application.

High PurityCAS [59-23-4]

50G250G

C6H12O6

PurityMW 180.16

>99.0%500G

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A2209 BEtA-GAlACtOSidASEFrom E. coli. An enzyme responsible for the degradation of galactosyl b-linkages in glycoproteins, glycolipids and polysaccharides. Can be conjugated to a variety of probes for the chromogenic detection of target sequences using X-Gal.

ConjugationCAS [9031-11-2] Store: -20°C

1KU5KU

Activity (Protein) at 37 °CAggregation

>550U/mg<3.0%

A2703 GElAtiN Reagent 100GBloompH at 25°CE. coliMoistureViscosity

238-2824.0-5.7

None<13%

38-50mps

500G

A2704 GENtAmyCiN SulFAtE (GENtAmyCiN)Aminoglycoside antibiotic. Inhibits gram-, gram+ bacteria and mycoplasma by blocking protein synthesis. Soluble in water 50mg/ml, typical working concentration: 15μg/ml.

USPCAS [1405-41-0]Store: 4°C

1G5G

10GPotencypH (4%, Water) at 25°CResidue on IgnitionLoss on DryingLimit of MethanolEndotoxins

>590μg/mg3.5-5.5<1.0%

<18.0%<1.0%

<0.71EU/mgA2420 d-GluCOSE, ANHydrOuS

Sugar supplement to media for specialized application.BiotechnologyCAS [50-99-7]

500G1KG

C6H12O6 Purity InsolublesLoss on Drying Residue on Ignition Chloride Sulfate & SulfiteIronArsenicHeavy Metals (as Pb) DNase, RNase & Protease

MW 180.16>99.8%

<0.005%<0.2%

<0.02%<0.01%

<0.005%<0.0005%

<0.00004%<0.0005%

None Detected

5KG

A2707 GluCOSE OXidASE, >100u/mGFrom Aspergillus Niger. It is used in the determination of glucose biosensors; In the determination of glucose in colorimetric assays in conjunction with Peroxidase; For use as an enzyme label, e.g. in ELISA systems. Solubility: Dissolves readily at 5mg/ml in 0.1M potassium phosphate buffer, pH 7.0, to give a yellow, clear solution.

High PurityCAS [9001-37-0]Store: -20°C

100KU250KU

1MU

A4149 GluCOSE OXidASE, >225u/mGFrom Aspergillus Niger. It is used in the determination of glucose biosensors; In the determination of glucose in colorimetric assays in conjunction with Peroxidase; For use as an enzyme label, e.g. in ELISA systems. Solubility: Dissolves readily at 5mg/ml in 0.1M potassium phosphate buffer, pH 7.0, to give a yellow, clear solution.

High PurityCAS [9001-37-0]Store: -20°C

50KU100KU250KU500KU

1MUA4147 β-GluCOSidASE

Dissolves readily at 5mg/ml in 0.1M sodium acetate buffer, pH5.0, to give a clear solution.

BiotechnologyCAS [9001-22-3]Store: -20°C

100KU500KU

Activity >1000U/mg materialA2710 GlutAtHiONE, OXidiZEd High Purity 1G

C20H32N6O12S2

PurityIronLeadLoss on Drying

MW 612.63>95.0%

<0.002%<0.001%

<8.0%

CAS [27025-41-8]Store: 4°C

5G

A2711 GlutAtHiONE, rEduCEd High Purity 1GC10H17N3O6SPurityIronLeadLoss on Drying

MW 307.33>98.0%

<0.002%<0.001%

<1.0%

CAS [70-18-8]Store: 4°C

5G 10G50G

250G

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A2713 GlyCErOlUsed in gel electrophoresis to facilitate samples to the bottom of the gel well. Used as a fixative in bacterial preservation media. Used as a protein stabilizer and storage buffer component.

BiotechnologyCAS [56-81-5]

250ML500ML

1L

C3H8O3

PurityHeavy Metals (as Pb) Water (KF)Residue on IgnitionDNase, RNase & Protease

MW 92.09>99.7%

<0.0005%<1.0%

<0.01%None Detected

A2715 GlyCiNE BiotechnologyCAS [56-40-6]

500G1KG5KG

C2H5NO2

PurityLoss on DryingSulfateChlorideHeavy MetalsDNase, RNase & Protease

MW 75.07>99.0%

<0.2%<0.05%<0.01%

<0.002%None Detected

A2716 GlyCiNESuitable for Electrophoresis.

High PurityCAS [56-40-6]

500G1KG5KGC2H5NO2

PurityLoss on DryingSulfateChlorideHeavy Metals (as Pb)

MW 75.07>99.0%

<0.2%<0.05%<0.01%

<0.002%A4172 GlyCOGEN, 20mG/ml

From Oysters, for DNA precipitation. An analogue of starch, glycogen is used in many areas of research in order to recover nucleic acids. It is possible to obtain nanogram or picogram quantities of DNA and RNA by using glycogen.

Molecular BiologyStore: -20°C

1ML

A2717 GlyCylGlyCiNE High Purity 25GC4H8N2O3

Purity Heavy Metals (as Pb) ChlorideAmmoniaArsenicAshIronSulfateLoss on DryingSolubility (10%, Water)

MW 132.12>99.0%

<0.002%<0.02%<0.02%

<0.0001%<0.2%

<0.001%<0.03%

<0.2%Clear/Haze Free

CAS [556-50-3] 100G250G500G

A2719 GuANidiNE HydrOCHlOridEA strong denaturant used to solubilize proteins. Suited for purification of nucleic acids or polypeptides from cell sources and inhibition of both RNase and protease activity.

BiotechnologyCAS [50-01-1]

100G500G

CH5N3.HClPurityA260 (6M, Water)ArsenicIronLeadDNase , RNase & Protease

MW 95.53>99.5%

<0.03<0.0005%<0.0005%<0.0005%

None DetectedA2722 GuANidiNE tHiOCyANAtE

A powerful protein denaturant used to inactivate endogenous RNases during the isolation of RNA from tissues and bacterial cultures.

Biotechnology CAS [593-84-0]

50G100G250G500GCH5N3.HSCN

PurityA280 (70%, Water)A300 (6M, Water)A410 (6M, Water)CopperLeadDNase, RNase & Protease

MW 118.16>99.0%

<0.8<0.1<0.1

<0.0001%<0.0001%

None Detected

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A2724 GuANiNE Ultra Pure 5GC5H5N5OAssay (Anhydrous)

MW 151.13>97.0%

CAS [73-40-5] 10G25G

A2802 HEpES, FrEE ACidOne of the best general purpose buffers available for biological research. Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, CO2 fixation; Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates; Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials. Useful pH range: 6.8-8.2.

High PurityCAS [7365-45-9]

25G100G250G250G

C8H18N2O4SPuritypKa at 25°CHeavy MetalsDNase, RNase & Protease

MW 238.31>99.0%

7.35-7.75<0.001%

None DetectedA2803 HEpES, SOdium SAlt High Purity 25G

C8H17N2O4SNa PuritySolubility (5%, Water)pKa at 25°CHeavy Metals (as Pb)DNase, RNase & Protease

MW 260.30 >99.0%

Clear/Colorless7.45-7.65

<0.0001%None Detected

CAS [75277-39-3] 100G500G1KG

A2811 HydrOCHlOriC ACidMeets ACS grade. Density: 1.17g/ml.

ReagentCAS [7647-01-0]

100ML500ML

HClPurityResidue on IgnitionHeavy Metals

MW 36.4636.5-38.0%

<0.008%<0.0005%

UN 1789

A4190 HydrOGEN pErOXidE, 3%Stabilized

USPCAS [7722-84-1]UN 2014

500ML

H2O2

H2O2 ContentH2O Content

MW 34.01~3%

~97%

A2813 HyGrOmyCiN BCell culture tested. An aminoglycoside antibiotic that inhibits protein synthesis in bacteria, fungi and higher eucaryotic cells. Widely used as a selection agent for procaryotic and eucaryotic cells carrying the hygromycin resistance gene. Recommended working concentration 100 µg/ml. The working Hygromycin B concentration, for the purpose of selection, varies with cell type, media, growth conditions and cell metabolic rate. Recommended hygromycin concentration for the selection of resistant cells is 25-1000μg/ml. Commonly used concentrations for selection are 200μg/ml for mammalian cells, 20-200μg/ml for plant cells & bacteria cells and 200-1000μg/ml for fungi. Your optimum hygromycin concentration should be tested experimentally.

Cell CultureCAS [31282-04-9]Store: 4°CUN 2811

100MG1G5G

C20H37N3O13

Purity (HPLC)Potency

MW 527.52>90.0%

>1000μg/mgA2901 imidAZOlE Biotechnology 10G

C3H4N2

PuritypH (5%, Water) at 25°CMelting PointInsolublesSolubility (20%, Water)InsolublesDNase, RNase & Protease

MW 68.08>99.0%9.5-10.588-92oC

<0.1%Clear/Colorless

<0.1%None Detected

CAS [288-32-4]UN 3263

25G100G500G

A2902 iOdONitrOtEtrAZOlium CHlOridE [iNt dyE]Used to visualize dehydrogenase activity histochemically. Useful for Western Blot staining. Soluble in DMSO, methanol/water (1:1).

High PurityCAS [146-68-9] Store: -20°C

100MG500MG

1GC19H13N5O2IClPuritySolubility (1%, DMSO) (P/F)

MW 505.71>99.0%

Pass

5G

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A4170 iNdOlE-3-ButyriC ACid [iBA]Used in plant cell culture.

ReagentCAS [133-32-4]

1G5G

C12H13NO2

PurityHeavy Metals

MW 203.24>98.0

<0.001%

Store: 4°C 25G100G250G

A2903 iSOprOpyl-B-d-tHiOGAlACtOpyrANOSidE [iptG]Commonly used inducer for b-Galactosidase activity in many bacterial gene sequences controlled by the lac operon. IPTG is used in conjunction with X-Gal in the Blue/White colony screening assay.

Ultra PureCAS [367-93-1]Store: 4°C

1G5G

10G25G

100G C9H18O5SPurityMelting PointMositure (KF)Dioxane (P/F)pH (5%, Water) at 25°CSolubility (1%, Water) (P/F)

MW 238.31>99.0%

110-114°C<1.0%

None Detected5-7

Clear/Colorless

A2904 iSOAmyl AlCOHOlSuitable for use in nucleic acid purification. Isoamyl Alcohol is a common alcohol used in the purification of DNA. A small amount of Isoamyl Alcohol is often added to phenol chloroform mixtures to ensure deactivation of RNase activity and reduce foaming.

Ultra PureCAS [123-51-3] UN 1105

500ML1L

C5H12OPurityColor (APHA)Moisture (KF)

MW 88.15>99.0%

<20<1.0%

A2905 iSOprOpyl AlCOHOlPrecipitation of DNA with isopropanol is commonly used for concentrating, desalting and recovering purified DNA. Isopropanol precipitations are typically carried out at room temperature with minimal incubation times and are extremely useful during the isolation of large sample volumes of DNA.

BiotechnologyCAS [67-63-0]UN 1219

500ML1L4L

C3H7OHPurityColor (APHA)Moisture (KF)

MW 60.09>99.0%

<10<0.2%

A3102 KANAmyCiN SulFAtEStreptomyces kanamyceticus. Kanamycin is an aminoglycoside antibiotic that inhibits protein synthesis in gram- and gram+ bacteria and in mycoplasma. Recommended working concentration: 30μg/ml.

USPCAS [25389-94-0]

5G10G25G

100GC18H36N4O11.H2SO4

Potency (Anhydrous)pH (1%, Water) at 25°CLoss on Drying

MW 582.58>750μg/mg

6.5-8.5<4.0%

A3208 lEupEptiN, HEmiSulFAtEAc-Leu-Leu-argininal.1/2 H2SO4, Synthetic. A reversible inhibitor of cysteine proteases and serine proteases resembling trypsin. Inhibits trypsin, plasmin, papain, kallikrein, thrombin and cathepsin A and B. Soluble in water, methanol or 100% ethanol. Working concentration: 10-100μM.

BiotechnologyCAS [103476-89-7] Store: -20°C

5MG25MG

C20H38N6O4.1/2H2SO4

PurityMW 475.59

>95.0%A3216 litHium CHlOridE Biotechnology 100G

LiCl PurityInsolublesIron

MW 42.39>99.0%<0.01%<10ppm

CAS [7447-41-8] 500G1KG

A3222 lOAdiNG dyE, 5X, 30% GlyCErOlFor most DNA and RNA applications. Exclusive blend of three tracking dyes: Dye #1 - A light blue dye that migrates at 4000bp in 1% agarose; Dye #2 - An indigo dye that migrates at about 600bp in 1% agarose and Dye #3 - A magenta dye that migrates at 150bp in 1% agarose. DNase, RNase and Protease Free.

Ultra PureStore: 4°C

5ML

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A3223 lOAdiNG dyE, 6X, 60% GlyCErOl6x Gel Loading Dye is the most popular, pre-mixed loading buffer for agarose and non-denaturing poylacrylamide gel electrophoresis. It contains two tracking dyes: bromophenol blue and xylenecyanol. EDTA is included to chelate magnesium (up to 10mM) in enzymatic reactions, thereby stopping the reaction. No DNA masking during gel exposure to UV light. DNase, RNase and Protease Free.

Ultra PureStore: 4°C

5x1.4ML

A3224 lOAdiNG dyE, 6X, 15% FiCOllFor DNA and RNA gels. Exclusive blend of three tracking dyes: Dye #1 –A light blue dye that migrates at 4000bp in 1% agarose; Dye #2 –An indigo dye that migrates at about 600bp in 1% agarose and Dye #3 –A magenta dye that migrates at 150bp in 1% agarose. DNase, RNase and Protease Free.

Ultra Pure 5ML

A3233 lySOZymE, EGG wHitELysozyme is a hydrolytic enzyme specific for peptidoglycans found in the lipid bilayer of bacteria. This enzyme breaks down the cell walls of bacteria for subsequent nucleic acid extraction.

Ultra Pure Store: -20°CCAS [12650-88-3]

1G5G

10G25G

ActivityHeavy MetalsYeast/MoldStandard Plate Count

>20000U/mg<10ppm

<10 CFU/g<10 CFU/g

100G250G

A3303 mAGNESium CHlOridE, HEXAHydrAtE ACS 100GMgCl2.6H2OPurityInsolublesAmmoniumBariumCalciumIronManganeseNitratePhosphatePotassiumSodiumSulfateHeavy Metals (as Pb)

MW 203.2999.0-102.0%

<0.005%<0.002%<0.005%

<0.01%<0.0005%<0.0005%

<0.001%<0.0005%

<0.005%<0.005%<0.002%

<0.0005%

CAS [7791-18-6] 500G1KG

A4171 mAGNESium CHlOridE SOlutiON25mM. For PCR and enzymatic reactions. DNase & RNase free.

Molecular BiologyStore: -20°C

5X1.4ML

A3304 mAGNESium SulFAtE, HEptAHydrAtE ACS 100GMgSO4.7H2OPurityInsolublesAmmoniumCalciumChlorideIronManganeseNitratePotassiumSodiumHeavy Metals (as Pb)

MW 246.4798.0-102.0%

<0.005%<0.002%

<0.02%<0.0005%<0.0005%<0.0005%

<0.002%<0.005%<0.005%

<0.0005%

CAS [10034-99-8] 500G1KG

A2430 dl-mAliC ACid High Purity 250GC4H6O5

PurityResidue on IgnitionInsolublesFumaric AcidMaleic Acid

MW 134.0999.0-100.5%

<0.1%<0.1%<1.0%

<0.05%

CAS [617-48-1] 500G2.5KG

A3307 mAltOSE, mONOHydrAtECommon media additive, particularly for the cultivation of lambda phage.

ReagentCAS [6363-53-7]

100G500G1KGC12H22O11.H2O

PurityLoss on DryingResidue after Ignition

MW 360.32>85.0%

<7.0%<0.1%

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A3308 mANGANESE SulFAtE, mONOHydrAtE Reagent 100GMnSO4.H2O PurityHeavy Metals (as Pbs)Insolubles

MW 169.02>98.0%<0.05%<0.08%

CAS [10034-96-5] 500G

A2431 d-mANNitOlSuitable for cell culture.

USPCAS [69-65-8]

100G500G

C6H14O6

PurityMelting RangeLoss on DryingChlorideSulfateArsenic

MW 182.1796.0-101.50%

164-169°C<0.3%

<0.007%<0.01%

<0.0001%

2.5KG

A2432 d-mANNOSE High Purity 10GC6H12O6 PurityMelting RangeLoss on Drying

MW 180.16>99.0%

125-131°C<1.0%

CAS [3458-28-4] 25G100G500G

A2008 2-mErCAptOEtHANOlApprox. 14.3M liquid, Density: 1.11g/ml

BiotechnologyCAS [60-24-2]

100ML500ML

C2H6OSPurity WaterDNase , RNase & Protease

MW 78.13>99.0%

<0.5%None Detected

UN 2966

A4135 mES, FrEE ACid, mONOHydrAtE2-(N-Morpholino)ethanesulfonic Acid

Ultra PureCAS [145224-94-8]

25G50G

C6H13NO4S.H2OPurity pKa at 25°CDNase , RNase & Protease

MW 213.25>99.0%5.9-6.3

None Detected

100G500G

A4160 mEtHANOl Reagent 500MLCH3OHPurityWaterAcidity, wtAcetone, wtColor (APHA)

MW 32.04>99.85%

<0.1%<0.003%<0.003%

<5

CAS [67-56-1]UN 1230

1L

A3327 mEtHylENE BluE, CHlOridE, triHydrAtEPost-electrophoretic stain for DNA. Less sensitive than Ethidium Bromide, but non-intercalating. Also used to stain RNA in bacteriological procedures.

ReagentCAS [7220-79-3]

25G100G500G

C16H18ClN3S.3H2ODye Content (Dry Basis)ArsenicCopperLeadZinc Loss on Drying

MW 373.9198-103%

<0.0008%<0.02%

<0.001%<0.001%

<15.0%

A3329 miNErAl Oil, liGHt wHitEDensity: 0.834-0.854g/ml, suitable for overlaying aqueous reactions. RNase, DNase & Protease: none detected.

Molecular BiologyCAS [8042-47-5]

100ML500ML

1L

A4161 mitOmyCiN CMitomycin C is an antineoplastic antibiotic that inhibits DNA synthesis by cross-linking DNA at 5’-CpG-3’sequences. Targets gram+, gram-, and acid-fast bacteria. Used for preparation of STO feeder layers. Also used as a selection agent in cell culture. Soluble in water: 0.5mg/ml.

USPCAS [50-07-7]Store: 4°CUN 2811Store: 4°UN 2811

5MG10MG50MG

C15H18N4O5

AssayWater

MW 334.33>970μg/mg

<2.5%

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A3332 mOlyBdiC ACid, AmmONium SAlt, tEtrAHydrAtE ACS 100G(NH4)6Mo7 O24.4H2OMoO3

ChlorideMagnesiumNitratePhosphatePotassiumSodiumSulfateInsolublesHeavy Metals (as Pb)

MW 1235.8681.0-83.0%

<0.002%<0.005%<0.003%

<0.0005%<0.01%<0.01%<0.02%

<0.005%<0.001%

CAS [12054-85-2] 500G

A3333 mOlyBdiC ACid, SOdium SAlt, diHydrAtE ACS 100GNa2MoO4.2H2OPurityInsolubles Heavy Metals (as Pb) AmmoniumChlorideIronPhosphateSulfatepH (5%, Water) at 25°C

MW 241.9599.5-103.0%

<0.005%<0.0005%

<0.001%<0.005%<0.001%

<0.0005%<0.015%7.0-10.5

CAS [10102-40-6] 500G

A3335 mOpSMOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a buffering range from 6.5 - 7.9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

Ultra PureCAS [1132-61-2]

25G100G250G500G

C7H15NO4SPuritypKa at 25°CDNase, RNase & Protease

MW 209.27>99.0%7.0-7.4

None DetectedA3334 mOpS, SOdium SAlt

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a buffering range from 6.5 - 7.9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

High PurityCAS [71119-22-7]

50G100G250G

C7H14NO4S.NaPuritypH (0.1M, Water) at 25°CHeavy MetalspKa at 20°CLoss on DryingDNase, RNase & Protease

MW 231.25>99.0%

10.0-11.0<0.005%

7.0-7.4<1.0%

None DetectedA3338 mtt

Thiazolyl Blue Tetrazolium Bromide. Demonstrates oxidative systems by formation of a metal-formazan chelate precipitate.

Ultra PureCAS [298-93-1] Store: 4°C

250MG1G5G

C18H16N5SBrPurity

MW 414.32>98.0%

25G

A3339 myCOpHENOliC ACidFrom Penicillium brevi-compactum. Mycophenolic acid is a mammalian selection agent for cells expressing the E. coli gene for xanthine-guanine phosphosribosyl transferase. Mycophenolic acid suppresses cytokine-induced nitric oxide production by blocking inosine monophosphate dehydrogenase in the guanosine monophosphate pathway. Recommended working concentration: 25µg/ml in methanol. Used in mammalian expression. Inhibits inosinate dehydrogenase. Working range: 25μg/ml in methanol.

Ultra PureCAS [24280-93-1]Store: 4°C

50MG100MG500MG

C17H20O6

Purity MW 320.34

>98.0%

A3401 NAdB-Nicotinamide Adenine Dinucleotide, Trihydrate

ReagentCAS [53-84-9]

1G5G

C21H27O14N7P2.3H2OPurityMoisture (KF)

MW 717.47>97.0%

<4.0%

Store: -20°C 10G25G

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BiOCHEmiCAlS & ENZymES

A3402 NAdH B-Nicotinamide Adenine Dinucleotide, Reduced, Disodium Salt, Trihydrate

ReagentCAS [606-68-8] Store: -20°C

500MG1G5GC21H27O14N7P2Na2.3H2O

PurityMW 763.40

>92.0%A3404 NAlidiXiC ACid

Nalidixic acid is a bactericidal agent that inhibits DNA synthesis by blocking the activity of DNA gyrase in both gram+ and gram- bacteria. Recommended working concentration: 15µg/ml.

High PurityCAS [389-08-2]

5G10G25G

100GC12H12N2O3

PurityMelting PointLoss on DryingHeavy Metals (as Pb)

MW 232.24>98.0%

225-231°C<0.5%

<0.002%

A3408 NEOmyCiN SulFAtEInhibits protein synthesis in gram- and gram+ bacteria. Selection agent used to establish stable mammalian cell lines. Light sensitive. Protect from moisture. Solubility in water: 50mg/ml.

USPCAS [1405-10-3]

10G25G

100G

C23H46N6O13.3H2 SO4

Potency (Anhydrous)pH (3.3%, Water) at 25°CLoss on Drying

MW 908.90>600μg/mg

5.0-7.5<8.0%

A4120 NitrOBluE tEtrAZOlium CHlOridE [NBt]Used to enhance the color development of BCIP products in the determination of phosphatase activity.

Ultra PureCAS [298-83-9]Store: 4°C

500MG1G5G

C40H30N10O6Cl2PurityLoss on Drying

MW 817.65>98.0%<10.0%

A4188 O-NitrOpHENyl-β-d-GAlACtOpyrANOSidE [oNpG]Chromogenic substrate for the detection of beta-Galactosidase. Commonly used in ELISA. Read at 410 nm.

Ultra PureCAS [369-07-3] Store: -20°C

1G5G

C12H15NO8

Solubility (1%, Water) at 25°CMW 301.25

Pass

A3413 NONidEt p-40 SuBStitutESuitable for electrophoresis. Density: 1.05-1.07g/ml.

Reagent 25ML50ML

100MLRefractive indexHLB NumberSolubility (5%, Water)

1.48-1.5213.1Clear

500ML

A5115 OrANGE G, SOdium SAltSuitable for use as tracking dye in nucleic gel electrophoresis. Very valuable as a plasma stain. Also a great background stain for hematoxylin.

High PurityCAS [1936-15-8]

25G 100G

C16H10N2O7S2Na2

Dye contentMW 452.38

>80.0%A3604 pENiCilliN G, SOdium SAlt

Inhibits cell wall synthesis in gram+ bacteria.USPCAS [69-57-8]

25MU100MU

C16H17N2NaO4SPotency (Anhydrous)pH (6%, Water) at 25 °CLoss on Drying

MW 356.37>1500U/mg

5.0-7.5<1.5%

500MU

A3609 pEpStAtiN AAspartic acid protease inhibitor. Soluble in acetic acid, ethanol, methanol.

High PurityCAS [26305-03-3]Store: 4°C

5MG 25MG

C34N63N5O9

PurityMW 685.90

>90.0%A3608 pEpSiN 1:3000

Porcine Source

High PurityCAS [9001-75-6]

100G 500G

ActivityHeavy MetalsE. Coli (P/F)Salmonella (P/F)

1:3000-1:3500<0.004%

None DetectedNone Detected

Store: 4°C

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A3610 pErOXidASE, HOrSErAdiSH [Hrp]Activity >250 U/mg. Purity: RZ>3.0. Superior to alkaline phosphatase and ß-galactosidase conjugates due to the higher specific enzyme activity. Small molecular size (MW 40,000) allows excellent cellular penetration. Variety of substrates available. Ideal in immunoblotting and immunocytochemistry applications. Used as the reporter enzyme for supersignalchemiluminescent substrates. Conjugates compatible with a number of substrates.

High PurityCAS [9003-99-0] Store at -20°C

10KU25KU

100KU500KU

A3611 pH StANdArd, 10.0pH (25°C): 9.97-10.03, color coded, accuracy to NIST standards.

500ML

A3612 pH StANdArd, 4.0pH (25°C): 3.97-4.03, color coded, accuracy to NIST standards.

500ML

A3613 pH StANdArd, 7.0pH (25°C): 6.97-7.03, color coded, accuracy to NIST standards.

500ML

A3614 pH StANdArd KitKit contains 500ml each of pH4.0, pH7.0 and pH10.0 standards. Color coded, accuracy to NIST standards.

1KIT

A3651 pHENOl, CryStAlliNEIsolating nucleic acids from proteins is critical to the success of many molecular biology investigations. Extraction with phenol and phenol:chloroform mixtures is a universal method for purification of DNA. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation.

Ultra PureCAS [108-95-2] Store: 4°CUN 1671

100G500G

C6H6OPurityMelting PointHeavy Metals (as Pb)Moisture (KF)

MW 94.11>99.0%40-41°C<0.01%

<0.5%A3616 pHENOl rEd

A pH indicator (Yellow to Red; range 6.8-8.4). Soluble in ethanol, acetone, Insoluble in water.

ACSCAS [143-74-8]

5G25G

C19H14O5S MW 354.37A3619 pHENOl rEd, SOdium SAlt

A pH indicator (Yellow to Red; range 6.8 - 8.4). Soluble in water. ACSCAS [34487-61-1]

5G25G

C19H13O5SNa MW 376.37A3620 pHENylmEtHylSulFONyl FluOridE [pmSF]

An irreversible serine protease inhibitor of trypsin, chymotrypsin, kallikrein, subtilisin and thrombin. Also inhibits the cysteine protease papain.

High PurityCAS [329-98-6]UN 2928

5G25G

100GC6H5CH2SO2FPurityMelting RangeTLC

MW 174.19>99.0%91-95°C One Spot

A3621 pHOSpHAtE BuFFErEd SAliNE [pBS] pOwdErIdeal for cell culture applications. 1X PBS Solution contains 137mM Sodium Chloride, 2.7mM Potassium Chloride and 10mM Phosphate Buffer. Each pack prepares 1L of 10X concentrate.

Ultra Pure 1PK

pH (1.0%, Water) at 25°CConductivity (1.0%, Water)

7.3-7.513500-17000µmhos

A3602 pHOSpHAtE BuFFEr SAliNE [pBS] tABlEtS, 100mlPre-measured tablets for convenient preparation of 1X PBS solution, pH 7.3-7.5. Each tablet prepares 100 ml of a 1X PBS solution. 1X PBS Solution contains 10mM Phosphate Buffer, 137mM Sodium Chloride, and 2.7mM Potassium Chloride.

Biotechnology 50TABS200TABS

pH (100ml Water) at 25°C 7.3-7.5A3625 pipES, SOdium SAlt

Useful pH range: 6.1-7.5High PurityCAS [100037-69-2]

50G100G

C8H17N2O6S2.1.5NaPurityLoss on DryingpH (1%, Water) at 25°CpKa at 25°CChlorideHeavy Metals

MW 335.34>99.0%

<2.0%6.5-7.56.7-6.9<1.0%

0.0001%

500G

A3630 pOlyEtHylENE GlyCOl 3350 [pEG 3350]avg. MW 3,350

BiotechnologyCAS [25322-68-3]

500G1KG

Purity >99.0%

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A3631 pOlyEtHylENE GlyCOl 8000 [pEG 8000]avg. MW 8,000

BiotechnologyCAS [25322-68-3]

500G1KG

PuritypH (5%, Water) at 25°CDNase & RNase

>99.0%5.0-7.0

None DetectedA3633 pOlyViNylpyrrOlidONE [pVp]

Common reagent used in nucleic acid hybridization for the detection of DNA or RNA. avg. MW 40,000

High PurityCAS [9003-39-8]

100G500G1KG

Purity (Dry Basis)pH (5%, Water) at 25°C Heavy Metals

>99.0%3.0-7.0

<0.001%A3634 pONCEAu S

Used as a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF. High PurityCAS [6226-79-5]

10G25G50GC22H12N4Na4O13S4

Loss on DryingDNase, RNase & Protease

MW 760.61<6.0%

None DetectedA3637 pOtASSium ACEtAtE ACS 500G

C2H3O2KPuritypH ( 5%, Water) at 25°C CalciumChlorideIronMagnesiumPhosphateSodiumSulfateHeavy Metals

MW 98.14>99.0%6.5-9.0

<0.005%<0.003%

<0.0005%<0.002%<0.001%

<0.03%<0.002%

<0.0005%

CAS [127-08-2] 1KG2.5KG

A4174 pOtASSium ACEtAtE Biotechnology 500GK C2H3O2

PuritypH (5%, Water) at 25°C ChlorideSulfateHeavy Metals DNase & RNase

MW 98.14>99.0%6.5-9.0

<0.003%<0.002%

<0.0005%None Detected

CAS [127-08-2] 1KG2.5KG

A3638 pOtASSium CArBONAtE, ANHydrOuS ACS 250GK2CO3

PurityInsolubles ChlorideSulfur CompoundsSodiumCalciumIronMagnesiumHeavy MetalsPhosphate

MW 138.21>99. 0%<0.01%

<0.003%<0.004%

<0.02%<0.005%

<0.0005%<0.002%

<0.0005%<0.001%

CAS [584-08-7] 500G1KG

A4178 pOtASSium CHlOridE ACS 500GKClPurityInsolublespH (5%, Water) at 25 °CHeavy Metals BromideCalciumChlorate & Nitrate (as NO3)IodideIronMagnesiumPhosphateSodiumSulfate

MW 74.5599.0-100.5%

<0.005%5.4-8.6

<0.0005%<0.01%

<0.002%<0.003%<0.002%

<0.0003%<0.001%

<0.0005%<0.005%<0.001%

CAS [7447-40-7] 1KG5KG

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A3639 pOtASSium CHlOridE Biotechnology 500GKClPurityInsolublespH (5%, Water) at 25 °CHeavy Metals BromideCalciumChlorate & Nitrate (as NO3)IodideIronMagnesiumPhosphateSodiumSulfate DNase, RNase & Protease

MW 74.5599.0-100.5%

<0.005%5.4-8.6

<0.0005%<0.01%

<0.002%<0.003%<0.002%

<0.0003%<0.001%

<0.0005%<0.005%<0.001%

None Detected

CAS [7447-40-7] 1KG5KG

A3640 pOtASSium FErriCyANidE ACS 50G

K3Fe(CN)6

PurityInsolublesChlorideSulfateFerro Compounds

MW 329.25>99.0%

<0.005%<0.01%<0.01%<0.05%

CAS [13746-66-2] 100G500G

A3641 pOtASSium HydrOXidE Reagent 500G

KOH PurityHeavy MetalsMoistureArsenicPotassium Carbonate

MW 56.11>85.0%

<0.003%10-17%

<0.0003%<3.5%

CAS [1310-58-3]UN 1813

1KG5KG

A3642 pOtASSium iOdidE ACS 50G

KIPurityInsolublespH (5%, Water) at 25 °CBariumCalciumChloride & BromideMagnesiumPhosphateSodiumSulfateHeavy Metals (as Pb)

MW 166.00>99.0%

<0.005%6.0-9.2

<0.002%<0.002%

<0.01%<0.001%<0.001%<0.005%<0.005%

<0.0005%

CAS [7681-11-0] 100G 500G1KG

A3643 pOtASSium NitrAtE ACS 500G

KNO3 PurityInsolublespH (5%, Water) at 25 °CChlorideCalciumIodateIronMagnesiumSodiumPhosphateSulfate NitriteHeavy Metals (as Pb)

MW 101.10>99.0%

<0.005%4.5-8.5

<0.002%<0.005%

<0.0005%<0.003%<0.002%<0.005%

<0.0005%<0.003%<0.001%

<0.0005%

CAS [7757-79-1]UN 1486

1KG

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A3646 pOtASSium pHOSpHAtE, diBASiC, ANHydrOuS ACS 250G

K2HPO4

PurityInsolublespH (5%, Water) at 25 °CChlorideSulfateIronSodiumLoss on Drying Heavy Metals (as Pb)

MW 174.18>98.0%<0.01%8.5-9.6

<0.003%<0.005%<0.001%

<0.05%<1.0%

<0.0005%

CAS [7758-11-4] 500G2.5KG

A4175 pOtASSium pHOSpHAtE, diBASiC, ANHydrOuS Biotechnology 250G

K2HPO4 PurityInsolublespH (5%, Water) at 25 °CChlorideSulfateIronSodiumLoss on Drying Heavy Metals (as Pb)DNase, RNase & Protease

MW 174.18>98.0%<0.01%8.5-9.6

<0.003%<0.005%<0.001%

<0.05%<1.0%

<0.0005%None Detected

CAS [7758-11-4] 500G2.5KG

A3647 pOtASSium pHOSpHAtE, mONOBASiC, ANHydrOuS ACS 250G

KH2PO4 PurityInsolublesLoss on DryingHeavy MetalspH (5%, Water) at 25°CChlorideSulfateIronSodium

MW 136.09>99.0%<0.01%

<0.2%<0.001%

4.1-4.5<0.001%<0.003%<0.002%<0.005%

CAS [7778-77-0] 500G2.5KG

A4176 pOtASSium pHOSpHAtE, mONOBASiC, ANHydrOuS Biotechnology 250G

KH2PO4 PurityInsolublesLoss on DryingHeavy MetalspH (5%, Water) at 25°CChlorideSulfateIronSodium DNase, RNase & Protease

MW 136.09>99.0%<0.01%

<0.2%<0.001%

4.1-4.5<0.001%<0.003%<0.002%<0.005%

None Detected

CAS [7778-77-0] 500G2.5KG

A3650 prOtEiNASE KProteinase K is a non-specific serine protease with a molecular weight of approx. 28,500. It is used in the following applications: inactivation of RNase and DNases, isolation of high molecular weight DNA, isolation of RNA or the isolation of plasmid and genomic DNA. Proteinase K is active with or without the presence of SDS, EDTA and Urea.

BiotechnologyCAS [39450-01-6]Store: -20°C

100MG500MG

1G5G

Activity

DNase & RNase

>30U/mg

None Detected

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A4168 purOmyCiN, diHydrOCHlOridEFrom Streptomyces alboniger. Cell culture tested. Puromycin is an aminoglysidic antibiotic. Puromycin is a protein inhibitor by inhibiting translation. Puromycin’s main function is used in cell biology as a selective antibiotic agent in cell culture systems. Puromycin is an antibiotic used for selecting mammalian cell lines, which have been transformed by vectors that express puromycin-N-acetyl-transferase (PAC). It is toxic to procaryotic & eukaryotic cells. Fast acting nucleoside antibiotic that inhibits protein synthesis. Soluble in water 50mg/ml, in methanol 20mg/ml. In powder form it is stable for more than one year.

BiotechnologyCAS [58-58-2]Store: -20°C

10MG25MG

100MG500MG

C22H29N7O5.2HClPurity (HPLC)WaterMelting Point

MW 544.43>99.0%<12.0%

168-178°CA3804 riBOFlAViN (VitAmiN B2) High Purity 25G

C17H20N4O6

Purity Ash

MW 376.37>98.0%

<0.3%

CAS [83-88-5] 100G

A3806 rNASE A (rBiONuClEASE A)RNase A (Ribonuclease A) is an endoribonuclease that efficiently hydrolyzes RNA. RNase A is used for a variety of molecular biology applications, most commonly for the hydrolysis of RNA that contaminates DNA preparations. This enzyme is used during plasmid purification without nicking or degrading plasmid DNA.

Molecular BiologyCAS [9001-99-4]Store: -20°C

100MG500MG

1G5G

10G Activity >70U/mg

A3808 rNASE iNHiBitOrRecombinant RNase inhibitor purified from E. coli expressing the gene from Sus domesticus. MW 40-66.2.

BiotechnologyStore: -20°C

2KU10KU

DNase & RNase None Detected

A3904 SilVEr NitrAtE Staining protein after electrophoretic separation. Also used to determine chloride ions in solution. Light sensitive.

ACSCAS [7761-88-8]UN 1493

10G25G

100GAgNO3

PurityChlorideSulfateCopperIronLead

MW 169.87>99.0%

<0.0005%<0.002%

<0.0002%<0.0002%

<0.001%A3905 SOdium ACEtAtE, ANHydrOuS ACS 500G

C2H3O2NaPurityInsolublesChlorideCalciumIronPhosphateSulfateLoss on DryingHeavy Metals (as Pb)

MW 82.03>99.0%<0.01%

<0.002%<0.005%<0.001%<0.001%<0.003%

<1.0%<0.001%

CAS [127-09-3] 1KG

A4177 SOdium ACEtAtE, ANHydrOuS Biotechnology 500GC2H3O2NaPurityInsolublesChlorideCalciumIronPhosphateSulfateLoss on DryingHeavy Metals (as Pb) Solubility (1M, Water)DNase, RNase & Protease

MW 82.03>99.0%<0.01%

<0.002%<0.005%<0.001%<0.001%<0.003%

<1.0%<0.001%

Clear/ColorlessNone Detected

CAS [127-09-3] 1KG

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BiOCHEmiCAlS & ENZymES

A3907 SOdium AZidE High Purity 50G

NaN3

Purity

Heavy Metals

Iron

Loss on Drying

Solubility (20%, Water)

DNase & RNase

MW 65.01

>99.0%

<0.002%

<0.001%

<0.5%

Clear/Colorless

None Detected

CAS [26628-22-8]UN 1687

250G

A4179 SOdium BiCArBONAtE, ANHydrOuS ACS 500GNaHCO3

Purity

Calcium

Chloride

Iron

Magnesium

Potassium

Sulfur Compounds

Phosphate

Heavy Metals(as Pb)

Insolubles

MW 84.01

99.7-100.3%

<0.02%

<0.003%

<0.001%

<0.005%

<0.005%

<0.003%

<0.001%

<0.0005%

<0.015%

CAS [144-55-8] 1KG

A3908 SOdium BiCArBONAtE, ANHydrOuS Biotechnology 500GNaHCO3

PurityCalciumChlorideIronMagnesiumPotassiumSulfur CompoundsPhosphateHeavy Metals (as Pb)Insolubles DNase, RNase & Protease

MW 84.0199.7-100.3%

<0.02%<0.003%<0.001%<0.005%<0.005%<0.003%<0.001%

<0.0005%<0.015%

None Detected

CAS [144-55-8] 1KG

A3909 SOdium BOrAtE, dECAHydrAtE Boric Acid, Sodium Decahydrate.

ReagentCAS [1303-96-4]

500G

Na2B4O7.10H2OPurityHeavy Metals

MW 381.3799.0-105.0%

<0.002%

A3910 SOdium CArBONAtE, ANHydrOuS ACS 500GNa2CO3 PurityInsolublesChloridePotassiumCalciumChloridePotassiumSilicaSulfur CompoundsIronHeavy Metals (as Pb)

MW 105.99>99.5%<0.01%

<0.001%<0.005%

<0.03%<0.001%<0.005%<0.005%<0.003%

<0.0005%<0.0005%

CAS [497-19-8]

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Code Product Description Size

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A3911 SOdium CHlOridE Biotechnology 500GNaCl

Purity

Phosphate

Calcium

Magnesium

Iron

Sulfate

Iodide

Nitrogen Compounds

Heavy Metals

DNase, RNase & Protease

MW 58.44

>99.9%

<0.0005%

<0.005%

<0.005%

<0.0002%

<0.0004%

<0.002%

<0.001%

<0.0005%

None Detected

CAS [7647-14-5] 1KG5KG

A3912 SOdium CHlOridE High Purity 500GNaCl

Purity

Phosphate

Calcium

Magnesium

Iron

Sulfate

Iodide

Nitrogen Compounds

Heavy Metals

MW 58.44

>99.9%

<0.0005%

<0.005%

<0.005%

<0.0002%

<0.0004%

<0.002%

<0.001%

<0.0005%

CAS [7647-14-5] 1KG5KG

A3901 SOdium dOdECyl SulFAtE [SdS] Biotechnology 100GC12H25NaO4S PuritySolubility (10%, Water)A260

(3%, Water)A280 (3%, Water)ChlorideCopperHeavy Metals (as Pb)IronPhosphateDNase, RNase & Protease

MW 288.38>99.0%

Clear/Colorless<0.10%<0.10%<0.10%

<0.0005%<0.0005%<0.0001%<0.0005%

None Detected

CAS [151-21-3]UN 1325

500G

A3903 SOdium dOdECyl SulFAtE [SdS] Reagent 100GC12H25NaO4S PurityInsolublesSolubility (10%, Water)

MW 288.38>99.0%

<0.003%Clear/Colorless

CAS [151-21-3]UN 1325

500G

A3913 SOdium HydrOXidE, pEllEtS ACS 500G

NaOHPuritySodium CarbonateChlorideCalciumPhosphateSulfateHeavy Metals IronMercuryNickelPotassium

MW 40.00>97.0%

<1.0%<0.005%<0.005%<0.001%<0.003%<0.002%<0.001%

<0.00001%<0.001%

<0.02%

CAS [1310-73-2]UN 1823

1KG

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Code Product Description Size

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BiOCHEmiCAlS & ENZymES

A4187 SOdium HydrOXidE, BEAdS Reagent 500GNaOHPuritySodium CarbonateChlorideSulfateHeavy Metals Potassium

MW 40.00>98.5%<0.90%<0.05%

<0.005%<0.006%

<0.1%

CAS [1310-73-2]UN 1823

1KG

A3914 SOdium iOdidE Ultra Pure 100GNaI PurityHeavy MetalsIronCopperSulfatePhosphate

MW 149.89>99.0%

<0.0005%<0.0003%<0.0003%

<0.005%<0.001%

CAS [7681-82-5] 500G

A3916 SOdium NitrAtE High Purity 500GNaNO3 Purity Insolubles pH (5%, Water) at 25°C

MW 84.99>99.0%<0.04%5.5-8.3

CAS [7631-99-4]UN 1498

1KG

A3920 SOdium pHOSpHAtE, diBASiC, ANHydrOuS ACS 500GNa2HPO4

Purity InsolublespH (5%, Water) at 25°CChlorideSulfateIronChlorideLoss on DryingHeavy Metals (as Pb)

MW 141.96>99.0%<0.01%8.7-9.3

<0.002%<0.005%<0.002%<0.002%

<0.2%<0.001%

CAS [7558-79-4] 1KG

A4133 SOdium pHOSpHAtE, mONOBASiC, mONOHydrAtE ACS 500GNaH2PO4.H2OPurityInsolublespH (5%, Water) at 25 °CHeavy MetalsCalciumChlorideIronPotassiumSulfate

MW 137.9998.0-102.0%

<0.01%4.1-4.5

<0.001%<0.005%

<0.0005%<0.001%

<0.01%<0.003%

CAS [10049-21-5] 1KG

A3922 SOdium SulFAtE, ANHydrOuS ACS 500GNa2SO4

PurityInsolublesHeavy Metals (as Pb) Residue on IgnitionpH (5%, Water) at 25 °CChlorideIronPhosphateCalciumMagnesiumPotassium

MW 142.04>99.0 %<0.01%

<0.0005%<0.5%5.2-9.2

<0.001%<0.001%<0.001%

<0.01%<0.005%

<0.01%

CAS [7757-82-6] 2.5KG

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Code Product Description Size

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A2450 d-SOrBitOl High Purity 100GC6H14O6

Purity

Moisture (KF)

Heavy Metals (as Pb)

Residue on Ignition

Chloride

Sulfate

Arsenic

MW 182.17

>98.0 %

<1.0%

<0.0001%

<0.1%

<0.005%

<0.01%

<0.0003%

CAS [50-70-4] 500G

A3929 StrEptAVidiNStreptomyces avidinii. A 60 kDa tetrameric protein that exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit, streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting procedures using biotin-labeled antibodies and enzymes. Unit definition: One unit will bind 1.0μg biotin.

Biotechnology CAS [9013-20-1] Store: -20°C

1MG5MG

ActivityDNase, RNase & Protease Electrophoresis

>17U/mg ProteinNone Detected

One Band

A3931 StrEptOmyCiN SulFAtEAntimicrobial inhibiting prokaryotic protein synthesis in gram- and gram+ bacteria.

Gram+/Gram-: Binds to the 30S subunit of bacterial ribosome. Working concentration:

30μg/ml. Recommended usage: for cell culture 100mg/L. Stability: Sterile filtered stock

solutions store at 4°C, 1month; at -20°C for longer stability.

USP CAS [3810-74-0]Store: 4°C

25G100G250G

(C21H39N7O12)2 .3H2SO4

Potency (Dry Basis)

pH (20%, Water) at 25°C

MW 1457.38

650-850mg/mg

4.5-7.0

A3932 SuCCiNiC ACid High Purity 100GC4H6O4

PurityHeavy Metals (as Pb)Residue on Ignition

MW 118.09>99.0%

<0.001%<0.025%

CAS [110-15-6] 500G

A3934 SuCrOSE Ultra Pure 500GC12H22O11

PurityInsolubles Heavy Metals (as Pb)IronChlorideGlucoseIronSulfateResidue on IgnitionLoss on Drying

MW 342.30>99.9%

<0.005%<0.0005%<0.0005%

<0.005%<0.1%

<0.0005%<0.005%

<0.01%<0.03%

CAS [57-50-1] 1KG

A3933 SuCrOSE Biotechnology 500GC12H22O11

PurityInsolubles Heavy Metals (as Pb)IronChlorideGlucoseIronSulfateResidue on IgnitionLoss on Drying DNase, RNase & Protease

MW 342.30>99.9%

<0.005%<0.0005%<0.0005%

<0.005%<0.1%

<0.0005%<0.005%

<0.01%<0.03%

None Detected

CAS [57-50-1] 1KG

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BiOCHEmiCAlS & ENZymES

A1113 t4 dNA liGASE5 Weiss U/µl, supplied with 10x Reaction Buffer. One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of 32P from pyrophosphate to ATP, into Norit-adsorbable material in 20 minutes at 37°C. One Weiss Unit is equivalent to approximately 67 cohesive-end ligation units.

Molecular BiologyStore: -20°C

200U1KU5KU

A4008 tEmEd Ultra Pure 25MLC6H16N2

Purity (Anhydrous Basis)Reactive IndexBoiling Range

MW 116.21>99.0%

1.417-1.419119-121°C

CAS [110-18-9]UN 2372

50ML100ML

A4010 tEtrACyCliNE HydrOCHlOridEInhibits protein synthesis in gram+ and gram- bacteria. Water soluble. Recommended

working concentration: 15µg/ml. Stock solution: 10mg/ml.

USPCAS [64-75-5]

25G100G

C22H24N2O8.HCl

Potency (Anhydrous)

pH (1%, Water) at 25°C

Loss on Drying

Heavy Metals

MW 480.90

>900µg/mg

1.8-2.8

<2.0%

<0.005%

A4128 tmB (3,3’ 5,5’-tEtrAmEtHyl BENZidiNE) diHydrOCHlOridEA sensitive substrate for the detection of horseradish peroxidase activity. Absorbs at

450 nm (yellow end-product). Ideal for ELISA and solution assays.

High PurityCAS [64285-73-0]Store: 4°C

500MG1G5G

C16H20N2.2HCl

Purity

Ash

Loss on Drying

MW 313.27

>98.0%

<0.1%

<2.0%

A4180 tHiAmiNE, HydrOCHlOridE (VitAmiN B1)Suitable for cell culture.

High PurityCAS [67-03-8]

5G25G

C12H17CIN4OS.HCl

Purity

MW 337.27

>98.0%

100G

A4018 triCiNEUseful pH range: 7.4-8.8

Ultra PureCAS [5704-04-1]

100G500G

C6H13NO5

Purity

Lead

DNase, RNase & Protease

MW 179.17

>99.0%

<0.001%

None Detected

A4020 triS Tris(hydroxymethyl)aminomethane. Reagent for stabilization of buffer formulations and

electrophoresis of biological molecules.

BiotechnologyCAS [77-86-1]

500G1KG

2.5KG

C4H11NO3

Purity

Insolubles

A280 (1M, Water)

A290 (1M, Water)

A400 (1M, Water)

Copper

Arsenic

Iron

Lead

Magnesium

DNase, RNase & Protease

MW 121.14

>99.9%

<0.005%

<0.05%

<0.2%

<0.05%

<0.0001%

<0.0005%

<0.0001%

<0.0001%

<0.0005%

None Detected

10KG

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A4021 triSTris(hydroxymethyl)aminomethane. Reagent for stabilization of buffer formulations and electrophoresis of biological molecules.

Ultra PureCAS [77-86-1]

500G1KG

2.5KGC4H11NO3

PurityInsolublesA280

(1M, Water)A290 (1M, Water)A400 (1M, Water)CopperArsenicIronLeadMagnesium

MW 121.14>99.9%

<0.005%<0.05%

<0.2%<0.05%

<0.0001%<0.0005%<0.0001%<0.0001%<0.0005%

10KG

A4019 triS ACEtAtE High Purity 25G

C4H11NO3 C2H4O2

PuritypH (0.1M, Water) at 25°CHeavy MetalsMoisture

MW 181.19>98.0%6.0-7.0

<0.001%<0.3%

CAS [6850-28-8] 100G500G

A4023 triS HydrOCHlOridEReagent for stabilization of buffer formulations and electrophoresis of biological molecules.

Ultra PureCAS [1185-53-1]

100G250G500G

C4H11NO3 .HClPurityInsolublesIronLeadCopperMagnesiumZincCalciumManganesepH (0.1M, Water) at 25°CDNase, RNase & Protease

MW 157.64>99.5%

<0.001%<0.001%

<0.0001%<0.0001%<0.0001%<0.0001%<0.0001%<0.0001%

4.2-4.9None Detected

5KG

A4025 tritON® X-100 Reagent 100MLC34H62O11

pH (5%, Water) at 25°CColor (APHA)

avg. MW 6476.0-8.0

<100

CAS [9002-93-1] 500ML1L

A4026 tritON® X-114 Reagent 100MLC14H22O(C2H4O)7-8

Color (APHA) Avg. MW 536

<100CAS [9036-19-5] 500ML

1L

A4029 trypSiNFrom Bovine Pancreas, activity >2500U/mg. Highly purified by affinity chromatography. Hymotrypsin <50 U/mg. The optimum pH is 8.0. Unit Definition: One unit hydrolyzes 1μM of p-toluene-sulfony-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 10mM calcium ion.

USPCAS [9002-07-7]Store: -20°C

1G5G

A4027 trypSiN 1: 250From Porcine Pancreas for single cell suspension.

Tissue CultureCAS [9002-07-7]

25G50G

TrypsinChymotrypsinLoss on Drying

>250U/mg<75U/mg

<5.5%

Store: 4°C 100G250G

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A4028 trypSiN 1: 300Porcine pancreas for single cell suspension.

Tissue CultureCAS [9002-07-7]

25G50G

Trypsin

Chymotrypsin

Loss on Drying

>300U/mg

<90U/mg

<5.5%

Store: 4°C 100G250G

A4181 trypSiN iNHiBitOr, SOyBEANA serine protease inhibitor specific for trypsin and trypsin-like proteases.

ReagentCAS [9035-81-8]Store: -20°C

500MG5G

25GActivity (BAEE Units) >7,000U/mg

A4031 twEEN® 20Polyoxyethylene-20-sorbitan monolaurate

ReagentCAS [9005-64-5]

100ML500ML

Hydroxyl Number

HLB Number

Moisture (KF)

Arsenic

Heavy Metals

Residue on Ignition

DNase, RNase & Protease

96-108

16.7

<3.0%

<0.0003%

<0.001%

<0.25%

None Detected

1L

A4032 twEEN® 80Polyoxyethylene-20-sorbitan monolaurate

ReagentCAS [9005-65-6]

100ML500ML

Hydroxyl Number

Moisture (KF)

Heavy Metals

Residue on Ignition

DNase, RNase & Protease

65-80

<3.0%

<0.001%

<0.25%

None Detected

1L

A1127 urACil dNA GlyCOSylASE [udG]Purified from E.coli strain K12. Catalyzes the hydrolysis of N-glycosylic bond between

uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-

stranded DNA. It shows no activity for RNA. 50 U/µl, supplied with 10x reaction buffer.

One unit of enzyme catalyzes the degradation of 1μg single-stranded uracil-containing

DNA in 60 minutes at 37°C.

Molecular BiologyCAS [117681-65-9]Store: -20°C

200U1KU

A4105 urEA Reagent 100GCH4N2O

Purity

Chloride

Cyanate

Iron

Heavy Metals (as Pb)

MW 60.06

>99.5%

<0.005%

<0.002%

<0.0001%

<0.0005%

CAS [57-13-6] 500G1KG5KG

A4103 urEA Ultra Pure 100GCH4N2O

Purity

Insolubles

Chloride

Cyanate

Iron

Heavy Metals (as Pb)

Conductivity (5M, Water)

DNase, RNase & Protease

MW 60.06

>99.5%

<0.005%

<0.0005%

<0.002%

<0.0001%

<0.0005%

<15µhmos

None Detected

CAS [57-13-6] 500G1KG5KG

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A4195 VANCOmyCiN HydrOCHlOridEAn amphoteric glycopepetide which inhibits cell wall synthesis in gram+ bacteria.

Recommended working concentration: 3µg/ml.

High PurityCAS [1404-93-9]

100MG250MG

1G5GC66H76Cl3N9O24

Potency (Dry Basis)

Moisture

pH (5%, Water) at 25°C

MW 1485.67

>900µg/mg

<5.0%

2.5-4.5

A4109 wAtEr, StErilE, rNASE FrEESuitable for PCR, tissue culture and most molecular biology applications. Sterilized to

eliminate any potential RNase and DNase..

Molecular BiologyCAS [7732-18-5]Store: 4°C

500ML

H2O

DNase & RNase

MW 18.02

None Detected

A4196 wAtEr, StErilESuitable for PCR, tissue culture and most molecular biology applications. Autoclave

sterile, endotoxin levels <0.1ng/ml.

Molecular BiologyCAS [7732-18-5] Store: 4°C

500ML

H2O

DNase

MW 18.02

None Detected

A4110 X-GAl5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside

Used in conjunction with IPTG to identify bacterial colonies that contain recombinant

plasmids by blue/white selection. X-Gal forms an intense blue precipitate in the

presence of b-Galactosidase.

Biotechnology CAS [7240-90-6]Store: -20°C

100MG250MG

1G5G

10GC14H15BrCINO6

Purity

Solution (1%, DMF)

MW 408.64

>99.0%

Clear/Colorless

A4113 X-GluC5-bromo-4-chloro-3-indolyl-b-D-glucuronic acid, cyclohexylammonium salt.

Chromogenic substrate for the detection of b-glucuronidase. Commonly used to detect

the GUS gene in bacterial colonies.

BiotechnologyCAS [18656-96-7]Store: -20°C

50MG100MG500MG

1GC14H13BrCINO7.C6H13N

Thin Layer Chromatogram

MW 521.80

One Spot

A4189 Xtt, SOdium SAltUsed to measure cell viability and growth.

Molecular BiologyCAS [111072-31-2]

100MG500MG

C22H16N7O13S2Na

Purity

MW 673.52

>90%

Store:4°C

A4115 XylENE CyANOl FFA tracking dye for DNA gels.

Ultra PureCAS [2650-17-1]

1G5G

10GC25H27N2O6S2Na MW 538.61

A4165 ZiNC SulFAtE, HEptAHydrAtE Reagent 100G500GZnSO4.7H2O

Purity

Arsenic

Lead

MW 287.54

99.0-108.7%

<0.0014%

<0.002%

CAS [7446-20-0]

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pCr, qpCr & rt-pCr

Over 98% of our orders are shipped from stock

2. PCR, qPCR & RT-PCR

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Code Description Size

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PC

R, q

PC

R &

RT-

PC

R

A1003 tAq dNA pOlymErASE5U/µl, supplied with 10x buffer (Mg2+ free) and 25mM MgCl2 solution.

500U

4X500U

Robust Taq for routine PCR use. It is ideal for amplification up to 5kb (simple template). Taq DNA Polymerase

is a highly thermostable DNA Polymerase isolated from the thermophilic bacterium Thermus aquaticus.

Amplification with Taq polymerase generates PCR products with 3’-A overhangs.

Store at -20°C.

10X500U

50x500U

100x500U

A4186 lONG-tAq dNA pOlymErASE5U/µl, supplied with 10x buffer (Mg2+ free) and 25mM MgCl2 solution.

250U

4x250U

Long-Taq is ideal for amplification up to 30kb (simple template). Applications: GC rich template, long template,

and sequencing. Amplification with this polymerase generates PCR products with 3’-A overhangs/blunt end.

Store at -20°C.

A4201 FASt-tAq dNA pOlymErASE5U/µl, supplied with 10x buffer (Mg2+ free) and 25mM MgCl2 solution.

250U

4x250U

Fast-Taq is an improved version of Taq DNA polymerase PCR which enables rapid PCR.

Store at -20°C.

A4144 HOtStArt tAq dNA pOlymErASE5U/µl, supplied with 10x buffer and 25mM MgCl2 solution.

100U

500U

HotStart Taq DNA Polymerase is a chemically modified Taq DNA Polymerase whose enzyme activities can

only be activated after 3-5 minutes of incubation at 94°C. This enzyme therefore inhibits polymerase reaction

before the onset of thermal cycling, preventing nonspecific DNA amplification and primer dimmer formation.

Amplification with this polymerase generates PCR products with 3’-A overhangs.

Store at -20°C.

250U

4X250U

A1006 pFu dNA pOlymErASE5U/µl, supplied with 10x buffer (Mg2+ free) and 25mM MgCl2 solution.

Not available in USA.

100U

500U

Pfu is ideal for high fidelity PCR amplification up to 2kb, blunt end vector cloning, RT-PCR and genotyping.

Pfu DNA polymerase is isolated from the pyrococcus furiosus. It is eight times more accurate than Taq DNA

polymerase, and highly thermostable - remains 95% active after 2 hours incubation at 95°C. Amplification

with Pfu DNA polymerase generates blunt-ended PCR products.

Store at -20°C.

A4145 tAq pCr mAStEr miX, 2XPacked 5x1ml, 25µl/Rxn.

200RXN

This Mix saves PCR preparation time by combining Taq, dNTPs and reaction buffer in a 2X concentrate

mixture, ready to use. It contains all the necessary reagents for amplification of DNA. To set up a PCR reaction,

simply add DNA template, primers and water. Amplification with this mix generates PCR products with 3’-A

overhangs.

Store at -20°C.

A4197 iGrEEN qpCr mAStEr miX-rOXPacked 4x1.25ml, 500RXN (20µL). For use in the following real-time PCR machines: ABI® 7000,7300,7700,7900,

StepOnePlus™, StepOne™, Eppendorf® Realplex 4.

500RXN

IGreen qPCR Master Mix-ROX contains internal passive reference dye (ROX). It is designed for quantitative

real-time analysis of DNA samples. The components of IGreen qPCR Master Mix have been developed for

superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The

chemically modified HotStart Taq polymerase included in the Master Mix significantly reduces non-specific

PCR amplification observed with regular Taq polymerase.

Store at -20°C.

pCr, qpCr & rt-pCr

Code Description Size

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A4183 iGrEEN qpCr mAStEr miX-lOw rOXPacked 4x1.25ml, 500RXN (20µL). For use in the following real-time PCR machines: ABI® 7500, Stratagene®

Mx3000, Mx3005, Mx4000.

500RXN

IGreen qPCR Master Mix-Low ROX contains internal passive reference dye (ROX). It is designed for quantitative

real-time analysis of DNA samples. It has been developed for superb performance in sensitivity, signal-to-

noise ratio, and complete elimination of primer dimers. The chemically modified HotStart Taq polymerase

included in the Master Mix significantly reduces non-specific PCR amplification observed with regular Taq

polymerase.

Store at -20°C.

A4184 iGrEEN qpCr mAStEr miX-iCyClErPacked 4x1.25ml, 500RXN (20µL). For use in the following real-time PCR machines: BioRad® iCycler®, iQ™5,

MyiQ™.

500RXN

IGreen qPCR Master Mix-iCycler contains internal passive reference dye (Fluorescein). It is designed for

quantitative real-time analysis of DNA samples. The components of IGreen qPCR Master Mix have been

developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer

dimers. The chemically modified HotStart Taq polymerase included in the Master Mix significantly reduces

non-specific PCR amplification observed with regular Taq polymerase.

Store at -20°C.

A4198 iGrEEN qpCr mAStEr miXPacked 4x1.25ml, 500RXN (20µL). For use in the following real-time PCR machines: BioRad® CFX96, Roche

LightCycler® 480, MJ Research Opticon™ and Opticon™ 2, MJ Research Chromo® 4, Corbett Rotor-gene®

600,3000, Eppendorf® Realplex 2.

500RXN

IGreen qPCR Master Mix does not contain any internal passive reference dye. It is designed for quantitative

real-time analysis of DNA samples. The components of EvaGreen qPCR Master Mix have been developed

for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The

chemically modified Hotstart Taq polymerase included in the Master Mix significantly reduces non-specific

PCR amplification observed with regular Taq polymerase.

Store at -20°C.

A5144 FirSt StrANd CdNA SyNtHESiS Kit (AmV) 20RXN

The Cloned AMV First-Strand cDNA Synthesis Kit is designed for the sensitive and reproducible detection

and analysis of RNA molecules in a two-step process. Cloned Avian Myeloblastosis Virus (AMV) Reverse

Transcriptase is purified to near homogeneity from baculovirus-infected insect cells containing the pol gene

of AMV.

Kit components: AMV Reverse Transcriptase, 5×AMV Reaction Buffer, dNTP Mix, Oligo-p(dT)18 Primer, Random

Primer p(dN)9, RNase Inhibitor, and RNase-free ddH2O.

Store at -20°C.

50RXN

A5146 FirSt StrANd CdNA SyNtHESiS Kit (m-mulV) 20RXN

The Cloned M-MULV First-Strand cDNA Synthesis Kit is designed for the sensitive and reproducible detection

and analysis of RNA molecules in a two-step process. Cloned Moloney Murine Leukemia Virus (M-MULV)

Reverse Transcriptase is purified to near homogeneity from baculovirus-infected insect cells containing the

pol gene of M-MULV.

Kit components: M-MuLV Reverse Transcriptase, 5x M-MuLV Reaction Buffer, RNase Inhibitor, dNTP Mix, Oligo-

(dT)18 Primer, Random Primer p(dN)6, RNase-free ddH2O.

Store at -20°C.

50RXN

Code Description Size

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A1111 m-mulV rEVErSE trANSCriptASE250 U/µl, supplied with 5x Reaction Buffer.

5KU

25KU

This enzyme is purified from an E.coli strain carrying a Moloney Murine Leukemia Virus (M-MuLV) reverse

transcriptase overproducing plasmid. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase.

This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA

synthesis) or single-stranded DNA as a template. M-MuLV Reverse Transcriptase lacks 3´ to 5´ exonuclease

activity. No detectable DNase and RNase contamination.

Store at -20°C.

A2405 dAtp 100mm SOlutiONStore at -20°C.

0.5ML

A2407 dCtp 100mm SOlutiONStore at -20°C.

0.5ML

A2422 dGtp 100mm SOlutiONStore at -20°C.

0.5ML

A2453 dttp 100mm SOlutiONStore at -20°C.

0.5ML

A2446 dNtp miX, 10mm EACH 0.5ML

The dNTP Mix is a ready-to-use aqueous solution containing dATP, dCTP, dGTP and dTTP, each at a final

concentration of 10mM.

Store at -20°C.

A2447 dNtp miX, 25mm EACH 0.5ML

The dNTP Mix is a ready-to-use aqueous solution containing dATP, dCTP, dGTP and dTTP, each at a final

concentration of 25mM.

Store at -20°C.

A4182 dNtp SEt, 100mm EACH KIT

dNTP Set contains 4 vials, 0.5ml each of the following: 100mM dATP; 100mM dCTP; 100mM dGTP and

100mM dTTP.

Store at -20°C.

Important Notice: The PCR process is covered by US. Patent numbers 4683195 and 4683202 issued to Cetus and owned by Hoffman-La Roche

Inc. Biomatik Corporation does not encourage or support the unauthorized use of the PCR process. Use of this product is recommended for

persons that either have a license to perform PCR or are not required to obtain a license.

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BioKits

Biomatik offers molecular biology kits for routine DNA and RNA isolation and purification needs. Our kits provide high purity DNA or RNA which are suitable for most of your downstream molecular biology applications.

SpinKlean pCr purification Kit

Code-Size K5101-100 prepsKit Components

Binding Solution

Wash Solution

Elution Buffer

SpinKlean Columns

Collection Tubes

50 ml

24 ml

10 ml

100

100

descriptionThe SpinKlean PCR Purification Kit provides an efficient and rapid

method for small scale purification of 60 bp to 10 kb DNA fragments

from PCR and other enzymatic reactions. This membrane based

system can bind up to 20 µg of DNA in as fast as 20 minutes. The

kit completely removes nucleotides, oligos, enzymes, mineral oil

and other impurities (such as agarose, proteins, salts, and ethidium

bromide during washing process).

Key FeaturesSimple & Fast: whole procedure in 20 minutes.

High Recovery: DNA yield is up to 95%.

High Capacity: up to 20 µg DNA in one preparation.

High Purity: for most downstream applications.

Hazard-Free: no phenol/chloroform is required.

ApplicationsPurified DNA from the SpinKlean PCR Purification Kit can

be used in the following applications: PCR and cloning,

Restriction enzyme digestion, Transformation, DNA

sequencing, In vitro transcription.

StorageStore at room temperature, for up to 1 year.

SpinKlean Gel dNA Extraction Kit

Code-Size K5111-100 prepsKit Components

Binding Solution

Wash Solution

Elution Buffer

SpinKlean Columns

Collection Tubes

60 ml

24 ml

10 ml

100

100

descriptionThe SpinKlean Gel DNA Extraction Kit provides efficient and rapid

method for small scale purification of DNA from agarose gel in TAE

or TBE buffers. This kit is designed to increase the recovery of DNA

fragments from 50 bp to 40 kb DNA fragments which are purified from

standard or low-melt agarose gels. This membrane based system can

bind up to 20 µg of DNA in as fast as 20 minutes. The kit completely

removes non-nucleic acid contaminants and inhibitors, such as

agarose, proteins, salts, and ethidium bromide during washing process.

Key FeaturesSimple & Fast: whole procedure in 20 minutes.

High Recovery: DNA yield is up to 85% in the range of

50 bp to 40 kb.

High Capacity: up to 20 µg DNA in one preparation.

High Purity: for most downstream applications.

Hazard-Free: no phenol/chloroform is required.

ApplicationsPurified DNA from the SpinKlean Gel Extraction Kit can

be used in the following applications: PCR and cloning,

Restriction enzyme digestion, Transformation, DNA

sequencing, In vitro transcription.

StorageStore at room temperature, for up to 1 year.

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SpinKlean plasmid dNA miniprep Kit

Code-Size K5113-100 prepsKit Components

Solution I

Solution II

Solution III

Wash Solution

Elution Buffer

RNase A (10 mg/ml)

SpinKlean Columns

Collection Tubes

24 ml

24 ml

50 ml

24 ml

10 ml

240 µl

100

100

descriptionThe SpinKlean Plasmid DNA Miniprep Kit provides efficient and

rapid method for small scale preparation of high quality DNA from

overnight recombinant E. coli. cultures. This membrane based

system can recover up to 20 µg of plasmid DNA in as fast as 30

minutes. The kit completely removes non-nucleic acid contaminants

and inhibitors.

Key FeaturesSimple & Fast: whole procedure in 30 minutes.

High Recovery: yield up to 95% of plasmid DNA.

High Capacity: up to 20 µg high copy plasmid DNA in

one preparation.

High Purity: for most downstream applications.

Hazard-Free: no phenol/chloroform is required.

ApplicationsPurified DNA from the SpinKlean Plasmid DNA Miniprep

Kit can be used in the following applications: PCR and

cloning, Restriction enzyme digestion, Transformation,

DNA sequencing, In vitro transcription.

StorageStore at room temperature, for up to 1 year.

(Keep RNase A solution at -20°C)

SpinKlean Genomic dNA Extraction Kit

Code-Size K5121-100 prepsKit Components

Solution I

Solution II

Wash Buffer I

Wash Buffer II

Elution Buffer

Proteinase K

SpinKlean Columns

Collection Tubes

30 ml

40 ml

60 ml

30 ml

10 ml

2 ml

100

100

descriptionThe SpinKlean Genomic DNA Extraction Kit provides simple and

rapid method for high quality genomic DNA purification from insect,

nematode, mammalian tissues etc. This membrane based system can

bind up to 30 µg of genomic DNA in as fast as 30 minutes.

Key FeaturesSimple & Fast: whole procedure in 30 minutes.

High Efficiency: up to 35 µg genomic DNA from 10 mg

of tissue.

High Purity: for most downstream applications.

Hazard-Free: no phenol/chloroform is required.

ApplicationsPurified DNA from the SpinKlean Genomic DNA

Extraction Kit can be used in the following applications:

PCR and cloning, Restriction enzyme digestion,

Labeling, In vitro transcription.

StorageStore at room temperature for up to 1 year.

(Keep Proteinase K solution at -20°C)

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triGent (total rNA isolation reagent)

Code-Size K5161-100 mlKit Components

Total RNA Isolation Reagent 100 ml

descriptiontriGent is equivalent to TRIZol. TRIGent is a ready-to-use reagent

for the isolation of total RNA from cells and tissues. The reagent, a

mono-phasic solution of phenol and guanidine isothiocyanate, is an

improvement to the RNA isolation method developed by Chomczynski

and Sacchi. During sample homogenization or lysis, TRIGent maintains

the integrity of the RNA, while disrupting cells and dissolving cell

components. TRIGent facilitates isolation of a variety of RNA specifies

of large or small molecular size. Starting material can be either fresh or

frozen tissue, or cell samples of almost any size.

rNA yield with different Starting materialsLive tissue 6-10 µg/mg tissue

Spleen tissue 6-10 µg/mg tissue

Kidney tissue 3-4 µg/mg tissue

Skeletal muscle 1-1.5 µg/mg tissue

Brain tissue 1-1.5 µg/mg tissue

Placenta 1-4 µg/mg tissue

Cultured epithelial cells 8-15 µg/106 cells

Fibroblasts 5-7 µg/106 cells

Key FeaturesClassical: Reliable and trusted method.

High Reproductivity: stable RNA yield.

High Purity: for any downstream applications.

Hazard-Free: no phenol/chloroform is required.

ApplicationsPurified DNA from the SpinKlean Genomic DNA

Extraction Kit can be used in many applications,

including: RT-PCR, Amplification for array analysis,

Hybridization assays, In vitro transcription.

StorageStore at 4°C for up to 1 year. Protect from light.

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4. Markers & Ladders

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dNA, rNA & protein markers & ladders

Biomatik offers a variety of high quality markers and ladders, ready to use,

which are ideal for precise quantitative analysis of DNA, RNA and Protein.

dNA ladders & markers Overview

Biomatik’s DNA ladders and markers are the industry standard for gel

electrophoresis. All of our DNA ladders and markers are quantitative - made

with precise DNA concentrations. PCR and plasmid digestion by restriction

enzymes are used for the production of DNA fragments for our ladders

and markers. All DNA fragments are exceptionally pure that are free of

any truncated or degraded molecules. Our DNA Ladders with sizes ranging

from 25 bp to 10 kb for use in agarose gel electrophoresis. Additionally,

we carry DNA markers for conventional agarose gel electrophoresis. All

the bands are clearly distinct using standard electrophoresis and evenly

distributed throughout the range. In addition to the highest product quality,

all DNA markers and ladders are highly competitive. These products are

stable during prolonged incubations at room temperature and multiple

freeze-thaw cycles.

With a collection of over 30 highly purified individual DNA fragments

ranging from 10-15,000 bp, Biomatik is able to custom make almost any

ladders and markers you would need, from microgram to gram! Contact

us for details.

Key Features of dNA ladders & markers:

• SuitableforanalysisofawiderangeofDNAs

• Bright,sharp,easy-to-identifybands

• Idealforquantitativeanalysis

• Stablefor1yearatroomtemperature

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markers & ladders for dNA, rNA and protein Sizing and quantitative Analysis

Code product Name Format Size loading range Conc. Bands

M7101 LMW DNA Ladder, Quantitative Ready to Use 100Loads 5µl/lane 25bp-700bp 104ng/µl 10

M7101 LMW DNA Ladder, Quantitative Ready to Use 5x100Loads 5µl/lane 25bp-700bp 104ng/µl 10

M7510 20bp DNA Ladder Plus, Quantitative Ready to Use 50Loads 5µl/lane 60bp-300bp 128ng/µl 13

M7510 20bp DNA Ladder Plus, Quantitative Ready to Use 5x50Loads 5µl/lane 60bp-300bp 128ng/µl 13

M7507 50bp DNA Ladder Plus, Quantitative Ready to Use 100Loads 5µl/lane 50bp-1kb 128ng/µl 13

M7507 50bp DNA Ladder Plus, Quantitative Ready to Use 5x100Loads 5µl/lane 50bp-1kb 128ng/µl 13

M7123 100bp DNA Ladder, Quantitative Ready to Use 100Loads 5µl/lane 100bp-1.5kb 100ng/µl 11

M7123 100bp DNA Ladder, Quantitative Ready to Use 5x100Loads 5µl/lane 100bp-1.5kb 100ng/µl 11

M7506 100bp DNA Ladder Plus, Quantitative Ready to Use 100Loads 5µl/lane 100bp-3kb 136ng/µl 14

M7506 100bp DNA Ladder Plus, Quantitative Ready to Use 5x100Loads 5µl/lane 100bp-3kb 136ng/µl 14

M7509 500bp DNA Ladder, Quantitative Ready to Use 100Loads 5µl/lane 500bp-5kb 92ng/µl 10

M7509 500bp DNA Ladder, Quantitative Ready to Use 5x100Loads 5µl/lane 500bp-5kb 92ng/µl 10

M7508 1Kb DNA Ladder, Quantitative Ready to Use 100Loads 5µl/lane 500bp-10kb 104ng/µl 10

M7508 1Kb DNA Ladder, Quantitative Ready to Use 5x100Loads 5µl/lane 500bp-10kb 104ng/µl 10

M7113 1Kb DNA Marker Plus, Quantitative Ready to Use 100Loads 5µl/lane 100bp-10kb 144ng/µl 15

M7113 1Kb DNA Marker Plus, Quantitative Ready to Use 5x100Loads 5µl/lane 100bp-10kb 144ng/µl 15

M6135 Lambda DNA/Hind III Marker Ready to Use 100Loads 6µl/lane 564bp-23130bp 83ng/µl 7

M6135 Lambda DNA/Hind III Marker Ready to Use 5x100Loads 6µl/lane 564bp-23130bp 83ng/µl 7

M6137 Lambda DNA/Hind III Plus Marker Ready to Use 100Loads 6µl/lane 300bp-23130bp 92ng/µl 10

M6137 Lambda DNA/Hind III Plus Marker Ready to Use 5x100Loads 6µl/lane 300bp-23130bp 92ng/µl 10

M7451 Low Range RNA Ladder Ready to Use 0.2ML 4µl/lane 100-1000 bases 0.25mg/ml 7

M7452 High Range RNA Ladder Ready to Use 0.2ML 4µl/lane 200-6000 bases 0.25mg/ml 8

M7433 Mid-Range Protein Marker Ready to Use 0.5ML 5µl/lane 10kDa-175kDa 11

M7442 Broad Range Protein Marker Ready to Use 0.5ML 5µl/lane 10kDa-245kDa 12

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lmw dNA ladder, quantitative, ready to use

Code-Size:Contents:Concentration: Band Concentration:

M7101-100 Loads (500µl), M7101-5x100 Loads (5x500µl)10 DNA Fragments (bp): 25, 50, 75, 100, 150, 200, 300, 400, 500, and 700520ng/5µl 100bp and 300bp Bands: 100ng/5µl; Other Bands: 40ng/5µl

description:LMW DNA Ladder is ideal for determining the size of double-stranded DNA from 25 to 700 base pairs. The ladder consists of 10 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 100bp and 300bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, all fragments except 100bp and 300bp are at 40ng. The 100bp and 300bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.

recommended loading: 5µl/Lane. Add 5μl ladder directly to each well. You may need more or less than 5μl of ladder, depending on well size and level of intensity needed to visualize the bands.

recommended Electrophoresis Condition: 5µl/Lane, 8cm, 3 % Agarose Gel, 0.5×TBE, 5V/cm, 1h.

Storage: 1 year at room temperature; for long term storage, please keep at -20 °C.

important Notes:Do not heat before loading.For quantification, adjust the concentration of the sample to equalize.Visualize DNA by staining with ethidium bromide or with SYBR Green I.

20bp dNA ladder, quantitative, ready to use

Code-Size:Contents:Concentration: Band Concentration:

M7510-50 Loads (250µl), M7510-5x50 Loads (5x250µl)13 DNA Fragments (bp): 60, 80, 100, 120, 140, 160, 180, 200, 220, 240, 260, 280, and 300640ng/5µl 100bp and 200bp Bands: 100ng/5µl; Other Bands: 40ng/5µl

description:20bp Ladder is ideal for determining the size of double-stranded DNA from 60 to 300 base pairs. The ladder consists of 13 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.

recommended loading: 5µl/Lane. Add 5μl ladder directly to each well. You may need more or less than 5μl of ladder, depending on well size and level of intensity needed to visualize the bands.

recommended Electrophoresis Condition: 5µl/Lane, 8cm, 3 % Agarose Gel, 1×TBE, 5V/cm, 1h.

Storage: 1 year at room temperature; for long term storage, please keep at -20 °C.

important Notes:Do not heat before loading.For quantification, adjust the concentration of the sample to equalize.Visualize DNA by staining with ethidium bromide or with SYBR Green I.

bp ng/5μl

—700 40

—500 40

—400 40

—200 40

—75 40—50 40

—25 40

5 μl/Lane. 8cm Gel0.5×TBE. 5V/cm. 1h

3% a

garo

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—150 40

—300 100

—100 100

bp ng/5μl

—300 40—280 40—260 40—240 40—220 40

—180 40—160 40

—140 40

—120 40

— 80 40

— 60 40

—200 100

—100 100

5 μl/Lane. 8cm Gel1×TBE. 5V/cm. 1h

4% a

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LMW DNA ladder

20bp DNA ladder

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50bp dNA ladder plus, quantitative, ready to use

Code-Size:Contents:Concentration: Band Concentration:

M7507-100 Loads (500µl), M7507-5x100 Loads (5x500µl)13 DNA Fragments (bp): 50, 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, and 1000640ng/5µl 250bp and 500bp Bands: 100ng/5µl; Other Bands: 40ng/5µl

description:50bp DNA Ladder Plus is ideal for determining the size of double-stranded DNA from 50 to 1000 base pairs. The ladder consists of 13 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 250bp and 500bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, all fragments except 250bp and 500bp are at 40ng. The 250bp and 500bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.

recommended loading: 5µl/Lane.Add 5μl ladder directly to each well. You may need more or less than 5μl of ladder, depending on well size and level of intensity needed to visualize the bands.

recommended Electrophoresis Condition: 5µl/Lane, 8 cm 3 % Agarose Gel, 0.5×TBE, 5V/cm, 1h.

Storage: 1 year at room temperature; for long term storage, please keep at -20 °C.

important Notes:Do not heat before loading.For quantification, adjust the concentration of the sample to equalize.Visualize DNA by staining with ethidium bromide or with SYBR Green I.

100bp dNA ladder, quantitative, ready to use

Code-Size:Contents:Concentration: Band Concentration:

M7123-100 Loads (500µl), M7123-5x100 Loads (5x500µl)11 DNA Fragments (bp): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, and 1500500ng/5µl 500bp Band: 100ng/5µl; Other Bands: 40ng/5µl

description:100bp DNA Ladder is ideal for determining the size of double-stranded DNA from 100 to 1500 base pairs. The ladder consists of 11 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 500bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, all fragments except 500bp are at 40ng. The 500bp fragment is at 100ng. This ladder is pre-mixed with loading dye and is ready to use.

recommended loading: 5µl/Lane. Add 5μl ladder directly to each well. You may need more or less than 5μl of ladder, depending on well size and level of intensity needed to visualize the bands.

recommended Electrophoresis Condition: 5µl/Lane, 8cm, 1.7% Agarose Gel, 0.5×TBE, 5V/cm, 1h.

Storage: 1 year at room temperature; for long term storage, please keep at -20 °C.

important Notes:Do not heat before loading.For quantification, adjust the concentration of the sample to equalize.Visualize DNA by staining with ethidium bromide or with SYBR Green I.

bp ng/5μl

—1000 40—900 40—800 40—700 40—600 40

—400 40

—300 40

—200 40

—150 40

—100 40

—50 40

5 μl/lane. 8cm Gel0.5×TBE. 5V/cm. 1h

50bp ladder plus

3% a

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—500 100

—250 100

100bp ladder

bp ng/5μl

—1500 40

—1000 40—900 40—800 40—700 40—600 40

—400 40

—300 40

—200 40

—100 40

—500 100

1.7%

aga

rose

5 μl/Lane. 8cm Gel0.5×TBE. 5V/cm. 1h

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100bp dNA ladder plus, quantitative, ready to use

Code-Size:Contents:Concentration: Band Concentration:

M7506-100 Loads (500µl), M7506-5x100 Loads (5x500µl)14 DNA Fragments (bp): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1200, 1500, 2000, and 3000680ng/5µl 500bp and 1200bp Bands: 100ng/5µl; Other Bands: 40ng/5µl

description:100bp DNA Ladder Plus is ideal for determining the size of double-stranded DNA from 100 to 3000 base pairs. The ladder consists of 14 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 500bp and 1200bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, all fragments except 500bp and 1200bp are at 40ng. The 500bp and 1200bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.

recommended loading: 5µl/Lane. Add 5μl ladder directly to each well. You may need more or less than 5μl of ladder, depending on well size and level of intensity needed to visualize the bands.

recommended Electrophoresis Condition: 5µl/Lane, 8cm 1.7 % Agarose Gel, 0.5×TBE, 5V/cm, 1h.

Storage: 1 year at room temperature; for long term storage, please keep at -20 °C. important Notes:Do not heat before loading.For quantification, adjust the concentration of the sample to equalize.Visualize DNA by staining with ethidium bromide or with SYBR Green I.

500bp dNA ladder, quantitative, ready to use

Code-Size:Contents:Concentration: Band Concentration:

M7509-100 Loads (500µl), M7509-5x100 Loads (5x500µl)10 DNA Fragments (bp): 500, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, and 5000460ng/5µl 2000bp Band: 100ng/5µl; Other Bands: 40ng/5µl

description:500bp ladder is ideal for determining the size of double-stranded DNA from 500 to 5,000 base pairs. The ladder consists of 10 linear double-stranded fragments .The 2000bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, all fragments except 2000bp are 40ng. The 2000bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.

recommended loading: 5µl/Lane. Add 5μl ladder directly to each well. You may need more or less than 5μl of ladder, depending on well size and level of intensity needed to visualize the bands.

recommended Electrophoresis Condition: 5µl/Lane, 8cm 1 % Agarose Gel, 1×TAE, 7V/cm, 45min.

Storage: 1 year at room temperature; for long term storage, please keep at -20 °C. important Notes:Do not heat before loading.For quantification, adjust the concentration of the sample to equalize.Visualize DNA by staining with ethidium bromide or with SYBR Green I.

100bp ladder plus

—3000 40

—2000 40

—1500 40

—1000 40—900 40—800 40—700 40—600 40

—400 40

—300 40

—200 40

—100 40

—1200 100

—500 100

5 μl/Lane. 8cm Gel0.5×TBE. 5V/cm. 1h

1.7%

aga

rose

500bp ladder

bp ng/5μl

—5000 40—4500 40—4000 40—3500 40—3000 40

—2000 40

—1500 40

—1000 40

—500 40

—2500 100

1% a

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se

5 μl/Lane. 8cm Gel1×TAE. 7V/cm. 45min

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1Kb dNA ladder, quantitative, ready to use

Code-Size:Contents:Concentration: Band Concentration:

M7508-100 Loads (500µl), M7508-5x100 Loads (5x500µl)10 DNA Fragments (bp): 500, 1000, 1500, 2000, 3000, 4000, 5000, 6000, 8000, and 10000520ng/5µl 2000bp and 5000 Bands: 100ng/5µl; Other Bands: 40ng/5µl

description:1Kb DNA ladder is ideal for determining the size of double-stranded DNA from 500 to 1000 base pairs. The ladder consists of 10 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 2000bp and 5000bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, all fragments except 2000bp and 5000bp are at 40ng. The 2000bp and 5000bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.

recommended loading: 5µl/Lane. Add 5μl ladder directly to each well. You may need more or less than 5μl of ladder, depending on well size and level of intensity needed to visualize the bands.

recommended Electrophoresis Condition: 5µl/Lane, 8cm, 1% Agarose Gel, 1×TAE, 7V/cm, 45min.

Storage: 1 year at room temperature; for long term storage, please keep at -20 °C.

important Notes:Do not heat before loading.For quantification, adjust the concentration of the sample to equalize.Visualize DNA by staining with ethidium bromide or with SYBR Green I.

1Kb dNA ladder plus, quantitative, ready to use

Code-Size:Contents:

Concentration: Band Concentration:

M7113-100 Loads (500µl), M7113-5x100 Loads (5x500µl)15 DNA Fragments (bp): 100, 200, 300, 400, 500, 700, 1000, 1500, 2000, 3000, 4000, 5000, 6000, 8000, and 10000620ng/5µl 500bp and 3000 Bands: 100ng/5µl; Other Bands: 40ng/5µl

description:1Kb DNA Ladder Plus is ideal for determining the size of double-stranded DNA from 100 to 10000 base pairs. The ladder consists of 15 linear double-stranded DNA fragments, chromatography-purified. The intensity of the 500bp and 3000bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, all fragments except 500bp and 3000bp are at 40ng. The 500bp and 3000bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.

recommended loading: 5µl/Lane. Add 5μl ladder directly to each well. You may need more or less than 5μl of ladder, depending on well size and level of intensity needed to visualize the bands.

recommended Electrophoresis Condition: 5µl/Lane, 8cm, 1% Agarose Gel, 1×TAE, 7V/cm, 45min.

Storage: 1 year at room temperature; for long term storage, please keep at -20 °C.

important Notes:Do not heat before loading.For quantification, adjust the concentration of the sample to equalize.Visualize DNA by staining with ethidium bromide or with SYBR Green I.

1kb ladder

bp ng/5μl

—10000 40—8000 40

—6000 40

—4000 40

—3000 40

—1500 40

—1000 40

—500 40

—5000 100

—2000 100

5 μl/Lane. 8cm Gel1×TAE. 7V/cm. 45min

1% a

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1kb ladder plusbp ng/5μl

—10000 40—8000 40—6000 40—5000 40—4000 40

—2000 40

—1500 40

—1000 40

—700 40

—400 40—300 40—200 40—100 40

—3000 100

—500 100

5 μl/Lane. 8cm Gel1×TAE. 7V/cm. 45min

1% a

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lambda dNA/ Hind iii marker, ready to use

Code-Size:Contents:Concentration:

M6135-100 Loads (600µl), M6135-5x100 Loads (5x600µl)7 DNA Fragments (bp): 564, 2027, 2322, 4361, 6557, 9416, and 23130500ng/6µl

0.7% agarose gel

description:Lambda DNA/ Hind III Marker is valuable for various DNA sizing purposes. Lambda DNA/Hind III Marker was digested with restriction enzyme Hind III, followed by phenol/chloroform extraction to inactivate the enzyme and dialyzed in TE buffer. The final DNA concentration is measured at OD260 and compared to other DNA standards for accuracy. This ladder is pre-mixed with loading dye and is ready to use.

recommended loading: 6µl/Lane. Add 6μl ladder directly to each well. You may need more or less than 6μl of ladder, depending on well size and level of intensity needed to visualize the bands.

recommended Electrophoresis Condition: 6µl/Lane, 8cm, 0.7% Agarose Gel, 0.5xTAE, 7V/cm, 45min.

Storage: 3 months at room temperature; for long term storage, please keep at -20 °C.

important Notes:Do not heat before loading.Visualize DNA by staining with ethidium bromide or with SYBR Green I.

lambda dNA/ Hind iii plus marker, ready to use

Code-Size:Contents:Concentration:

M6137-100 Loads (600µl), M6137-5x100 Loads (5x600µl)10 DNA Fragments (bp): 300, 564, 1000, 1500, 2027, 2322, 4361, 6557, 9416, and 23130550ng/6µl

0.7% agarose gel

description:Compared with the regular Lambda DNA/ Hind III Marker, Lambda DNA/Hind III Plus Marker has three extra unique DNA bands: 1500bp, 1000bp, and 300bp. The final DNA concentration is measured at OD260 and compared to other DNA standards for accuracy. 6μl contains 500ng of Hind III digested Lambda DNA and 50ng of the additional unique DNA bands. This ladder is pre-mixed with loading dye and is ready to use.

recommended loading: 6µl/Lane. Add 6μl ladder directly to each well. You may need more or less than 6μl of ladder, depending on well size and level of intensity needed to visualize the bands.

recommended Electrophoresis Condition: 6µl/Lane, 8cm, 0.7% Agarose Gel, 0.5xTAE, 7V/cm, 45min.

Storage: 3 months at room temperature; for long term storage, please keep at -20 °C.

important Notes:Do not heat before loading.Visualize DNA by staining with ethidium bromide or with SYBR Green I.

Size

— 23130bp— 9416bp— 6557bp— 4361bp

— 2322bp— 2027bp

— 564bp

Size

— 23130bp— 9416bp— 6557bp

— 4361bp

— 2322bp— 2027bp— 1500bp

— 1000bp

— 564bp

— 300bp

Lambda DNA/ Hind III Marker

Lambda DNA/ Hind III Plus Marker

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low range rNA ladder, ready to use

Code-Size: M7451-0.2ml (5x40µl, 50 Loads)Contents: 7 chromatography purified single-stranded RNA transcripts: 1000, 800, 600, 400, 300, 200 and 100 bases.Supplied with: 2X RNA Loading Dye, 1.0ml

description:Low Range RNA Ladder, ready-to-use, is a mixture of 7 chromatography purified single-stranded RNA transcripts (100-1000 bases), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments, free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see photo for precise quantities). To minimize the possibility of contamination during the use the ladder is aliquotted in smaller volumes. Ladder is premixed with loading dye and can be directly applied on both the native or denaturing agarose gels.

The ladder is designed for qualitative and quantitative analysis of RNA on agarose gels stained with ethidium bromide or SYBR® Green II. The ladder is free of degraded RNA and NTP’s. Therefore, spectrophotometric measurements provide accurate values of RNA concentration in each ladder band. Due to this feature, the Low Range RNA Ladder can be used for approximate RNA quantification on gels. The Low Range RNA Ladder, ready-to-use, is recommended for electrophoresis in the following: native 2% agarose with TAE buffer, denaturing formaldehyde agarose with MOPS buffer, denaturing glyoxal/DMSO agarose with sodium phosphate buffer and denaturing polyacrylamide gel electrophoresis in TBE buffer.

recommended loading: 4µl/Lane.

Storage: Store at -20°C for 6 months or at -70°C for longer periods.

High range rNA ladder, ready to use

Code-Size: M7452-0.2ml (5x40µl, 50 Loads)Contents: 8 chromatography purified single-stranded RNA transcripts: 6000, 4000, 3000, 2000, 1500, 1000, 500 and 200 bases.Supplied with: 2X RNA Loading Dye, 1.0ml

description:High Range RNA Ladder, ready-to-use, is a mixture of 8 chromatography purified single-stranded RNA transcripts (200-6000 bases), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments, free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see photo for precise quantities). To minimize the possibility of contamination during the use the ladder is aliquotted in smaller volumes. Ladder is premixed with loading dye and can be directly applied on both the native or denaturing agarose gels.

The ladder is designed for qualitative and quantitative analysis of RNA on agarose gels stained with ethidium bromide or SYBR Green II. The ladder is free of degraded RNA and NTP’s. Therefore, spectrophotometric measurements provide accurate values of RNA concentration in each ladder band. Due to this feature, the High Range RNA Ladder can be used for approximate RNA quantification on gels. The High Range RNA Ladder, ready-to-use, is recommended for electrophoresis in the following: native 1% agarose with TAE buffer, denaturing formaldehyde agarose with MOPS buffer, denaturing glyoxal/DMSO agarose with sodium phosphate buffer.

recommended loading: 4µl/Lane.

Storage: Store at -20°C for 6 months or at -70°C for longer periods.

bases ng/4μl bases

— 1000

— 1000

— 800

— 800

— 600

— 600

— 400

— 400

— 300 — 300— 200

— 200

— 200

— 200

140140140140

140140

140

4 μl/lane,8cm length gel1×TAE. 5V/cm

4 μl/lane,20cm length gel1×TBE. 8V/cm

2% a

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5.0%

pol

yacr

ylam

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urea

bases ng/4μl bases

— 6000

— 4000— 3000

— 2000— 1500— 1000

— 500

— 200

— 6000

— 4000

— 3000

— 2000— 1500— 1000

— 500

— 200

120

120120

120120

120

120

120

4µl/lane, 8cm length gel; 1x TAE, 5V/cm

1.0%

aga

rose

1.0%

form

alde

hyde

aga

rose

4µl/lane, 8cm length gel; 1x MOPS, 5V/cm

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mid-range protein marker, ready to use

Code-Size: M7433-0.5ml (100 Loads)Contents: 11 protein fragments, from 10kDA to 175kDa

description:Mid-Range Protein Marker consists of 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. This unique protein marker allows you to monitor protein separation during electrophoresis, estimate molecular weight of the protein of interest, and evaluate western blot transfer efficiency. It also includes ~10, ~40 and ~90 kDa reference bands coupled with blue dye that allow easy tracking and identification.

recommended loading: 5µl/Lane.

Storage: store at 4 °C for 3 months; for long term storage, please keep at -20 °C.

important Notes:This material is for laboratory research purpose and/or in vitro use only and is not to be used in humans or animals.

Broad range protein marker, ready to use

Code-Size: M7442-0.5ml (100 Loads)Contents: 12 protein fragments, from 10kDA to 245kDa

description:Broad Range Protein Marker consists of 12 proteins that resolve into sharp, tight bands in the range of 10-245 kDa. This unique protein ladder allows you to monitor protein separation during electrophoresis, estimate molecular weight of the protein of interest, and evaluate western blot transfer efficiency. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

recommended loading: 5µl/Lane.

Storage: store at 4 °C for 3 months; for long term storage, please keep at -20 °C.

important Notes:This material is for laboratory research purpose and/or in vitro use only and is not to be used in humans or animals.

~175 ~165 ~155~120~115

~62 ~62~52 ~55

~45

~26

~16

~12

~42

~24

~17

~13

~130

~70

~62

~51

~29

~22

Tris-Glycine15%

Bis-Tris 4~12%MOPS buffer

Bis-Tris 4~12%MES buffer

~14

~95~80 ~82

~32~34

~5~7

~42

~10.5

kDa kDa kDa

~245 ~235 ~240~165~125~93

~57

~42

~31

~18

~15

~8

~170~130~93~70 ~72

~24

~53

~41

~30

~18

~14~9

~22

~180~135

~100

~75

~63

~48

~35

~25

~20

~17

~11

Tris-Glycine4~20%

Bis-Tris 10%MOPS buffer

Bis-Tris 10%MES buffer

kDa kDa kDa

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5. Culture Media

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Culture media

Biomatik offers a selection of classical culture media for the maintenance and culture of microorganisms for genetic and molecular studies.

A8502 AGArSuitable for uses in most microbiological, tissue culture media and molecular biology applications. A solidifying agent typically used at concentrations of 1-2% in solid media preparations for the growth of bacteria and fungi. Agar consists of unbranched polysaccharides of galactose subunits derived from algae or seaweed. After autoclaving, a solution of 1.5% is a light amber color completely free of insoluble particles. Agar solidifies into a haze-free, firm-surface gel. Color: white or light cream.

Bacteriological CAS [9002-18-0]

100G500G

2.5KG

Pass Thru 60 Mesh Loss on DryingAshpH in Gel (1.5%)Turbidity (1.5%)Gel Strength (1.5%)Gel PointMelting PointHeavy Metal

>90%<15.0%<6.5%6.0-8.0

<50NTU550-1050g/cm2

32-38°C82-88°C

<0.004%

A8505 AGAr, NOBlE, puriFiEdA highly purified agar. This Agar is completely nutrient-free, most often used in defined media formulations where traces of nutrients can not be tolerated. Some labs use this purified agar as substitute of agarose.

Biotechnology CAS [9002-18-0]

50G100G500G

Pass Thru 60 Mesh Loss on DryingAshpH in Gel (1.5%)Turbidity (1.5%)Gel Strength (1.5%)Gel PointMelting Point

>95%<12.0%<6.5%6.0-7.5<6NTU

550-950g/cm2

32-38°C80-90°C

A8506 BEEF EXtrACtA dried replacement for infusion of meat. A completely water soluble powder.

High Purity 50G100G500GpH (10%, Water) at 25°C 4.6-6.0

A8507 CASAmiNO ACidSAcid hydrolyzed casein containing low concentration of sodium chloride and iron.

Bacteriological 100G500G

Total NitrogenAmino NitrogenAmino Nitrogen/Total NitrogenpH (10%, Water) at 25°CLoss on DryingAsh

>8.0%>6.5%>55%

5.5-7.0<6.0%<40%

A8523 lB AGAr (lENNOX)Used for the propagation and maintenance of E. coli. Widely used for the preparation of plasmid DNA and recombinant proteins. Light beige, free-flowing powder. Usage: 35g/L. Composition: Tryptone 10g/L, Yeast Extract 5g/L, Sodium Chloride 5g/L, and Agar 15g/L.

Tissue Culture 100G500G

A8517 lB AGAr (millEr)Used for the propagation and maintenance of E. coli. Widely used for the preparation of plasmid DNA and recombinant proteins.Usage: 40g/L. Composition: Tryptone 10g/L, Yeast Extract 5g/L, and Sodium Chloride 10g/L, and Agar 15g/L.

Tissue Culture 100G500G

A8521 lB BrOtH (lENNOX)Used for the propagation and maintenance of E. coli. Widely used for the preparation of plasmid DNA and recombinant proteins.Usage: 20gm/L. Composition: Tryptone 10g/L, Yeast Extract 5g/L, and Sodium Chloride 5g/L.

Tissue Culture 100G500G

2.5KG

Code Description Size

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A8515 lB BrOtH (millEr)Used for the propagation and maintenance of E. coli. Widely used for the preparation of plasmid DNA and recombinant proteins.Usage: 25gm/L. Composition: Tryptone 10g/L, Yeast Extract 5g/L, and Sodium Chloride 10g/L.

Tissue Culture 100G500G

2.5KG

A8536 mAlt EXtrACtUsed for the preparation of media for the propagation of yeasts and molds.

Bacteriological 100G500G

Total NitrogenLoss on DryingAshColiformpH (6%, Water) at 25°C

<2.0%<5.0%<3.0%<10/g

4.5-6.0

A8550 pEptONEAn enzymatic digestion of protein. Peptone is suitable for the preparation of general culture media. It contains at least 13.0% total nitrogen in a readily available form for bacteria and has peptides and amino acids content.

Bacteriological 100G500G

2.5KG

Total NitrogenpH (2%, Water) at 25°CLoss on DryingAsh

>13.0%6.5-7.5<5.0%<4.0%

A8551 pEptONE, SOyBEAN, ANimAl-FrEEAnimal-free Peptone is a papaic/pancreatic digest of defatted soybean meal derived from sources free of genetic modifications. It is an ideal substitute for peptone derived from animal sources for all growth media including tissue culture, fermentation and agar plates.

Bacteriological 100G500G

2.5KG

Total NitrogenpH (2%, Water) at 25°C

>8.0%6.5-7.5

A8570 SOB AGArA complex medium used for growing competent host cells prior to transformation. Usage: 42.5g/L. Composition: Tryptone 20g/L, Yeast Extract 5g/L, Sodium Chloride 0.5g/L, MgSO4×7H2O 5g/L and Agar 12g/L.

Biotechnology 100G500G

A8556 SOB BrOtHA rich medium used for growing competent host cells prior to transformation. Usage: 30.5g/L. Composition: Tryptone 20g/L, Yeast Extract 5g/L, Sodium Chloride 0.5g/L, and MgSO4×7H2O 5g/L.

Biotechnology 100G500G

A8562 SupEr BrOtHVersion of LB Broth containing increased amounts of tryptone and yeast extract. Usage: 60g/L. Composition: Tryptone 35g/L, Yeast Extract 20g/L, and Sodium Chloride 5g/L.

Biotechnology 100G500G

A8562 tErriFiC BrOtHUsed for the preparation of molecular genetic strains of E.coli. Supports higher density of cells to increase plasmid yield. Usage: 47.6g/L. Composition: Tryptone 12g/L, Yeast Extract 24g/L, Potassium Phosphate Monobasic 2.2g/L and Potassium Phosphate Dibasic 9.4g/L.

Biotechnology 100G500G

A8566 tryptONETryptone is the pancreatic digest of casein used as a nitrogen source for bacteria. This tryptone is the most popular one used in research labs for making medium.

Bacteriological 100G500G

2.5KGTotal NitrogenpH (6%, Water) at 25°CAmino NitrogenAshColiformLoss on Drying

>11.4%6.6-7.5>3.5%<8.0%<10/g

<6.0%

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A8569 yEASt EXtrACtThe water soluble portion of autolyzed yeast containing a source of Vitamin B complex. Suitable for cell culture and bacteriological use.

Bacteriological 100G500G

2.5KGpH (2%, Water) at 25°C 6.4-7.2

A8571 2X yt AGArUsage: 43g/L. Composition: Tryptone 16g/L, Yeast Extract 10g/L, Sodium Chloride 5g/L and Agar 12g/L.

Biotechnology 100G500G

A8572 2X yt BrOtHUsage: 31g/L. Composition: Tryptone 16g/L, Yeast Extract 10g/L and Sodium Chloride 5g/L.

Biotechnology 100G500G

A6201 l-AlANiNE High Purity 50GC3H7NO2

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 89.09>99.0%<0.1%<0.2%

<0.02%<0.001%

CAS [56-41-7] 250G1KG

A6203 l-ArGiNiNE High Purity 50GC6H14N4O2

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 210.66>98.5%<0.1%

<0.02%<0.5%

<0.001%

CAS [74-79-3] 250G1KG

A6205 l-ASpArAGiNE High Purity 50GC4H8N2O3

PurityLoss on DryingHeavy Metals

MW 132.12>99.0%<0.5%

<0.002%

CAS [70-47-3] 250G1KG

A6206 l-ASpArtiC ACid High Purity 50GC4H7NO4

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 133.11>99.0%<0.1%

<0.02%<0.03%

<0.001%

CAS [56-84-8] 250G1KG

A6257 l-CyStiNE High Purity 50GC6H12N2O4S2

PurityResidue on IgnitionLoss on DryingHeavy Metals

MW 240.30>98.5%<0.1%<0.2%

<0.001%

CAS [56-89-3] 250G1KG

A6207 l-CyStEiNE High Purity 50GC3H7NO2SPurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 121.15>98.0%<0.1%<0.5%

<0.03%<0.001%

CAS [52-90-4] 250G1KG

A6209 l-GlutAmiC ACid High Purity 50GC5H9NO4

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 147.13>98.5%<0.1%<0.1%

<0.02%<0.001%

CAS [56-86-0] 250G1KG

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Code Description Size

Over 98% of our orders are shipped from stock

A6210 l-GlutAmiNE High Purity 50GC5H10N2O3

PurityResidue on IgnitionLoss on DryingHeavy Metals

MW 146.15>98.5%

0.3%<0.3%

<0.001%

CAS [56-85-9] 250G1KG

A2716 GlyCiNE High Purity 500GC2H5NO2

PurityLoss on DryingSulfateChlorideHeavy Metals

MW 75.07>99.0%<0.2%

<0.05%<0.01%

<0.002%

CAS [56-40-6] 1KG5KG

A6211 l-HiStidiNE High Purity 25GC6H9N3O2

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 155.16>98.5%<0.1%<0.2%

<0.02%<0.001%

CAS [71-00-1] 100G500G

A6215 l-iSOlEuCiNE High Purity 25GC6H13NO2

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 131.18>98.5%

0.3%<0.3%

<0.03%<0.001%

CAS [73-32-5] 100G250G

A6217 l-lEuCiNE High Purity 50GC6H13NO2

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 131.17>98.5%<0.1%<0.2%

<0.02%<0.001%

CAS [61-90-5] 250G1KG

A6258 l-lySiNE High Purity 100GC6H14N2O2

PurityResidue on IgnitionLoss on DryingChlorideHeavy Metals

MW 146.19>98.5%<0.2%<0.5%<0.1%

<0.002%

CAS [56-87-1] 500G1KG

A6218 l-lySiNE, mONOHydrOCHlOridE High Purity 50GC6H14N2O2.HClPurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 182.65>99.0%<0.1%<0.4%

<0.02%<0.001%

CAS [657-27-2] 250G1KG

A6254 l-mEtHiONiNE High Purity 25GC5H11NO2SPurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 149.2198.5%<0.1%<0.2%

<0.02%<0.001%

CAS [63-68-3] 100G500G

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Code Description Size

A6225 l-pHENylAlANiNE High Purity 50GC9H11NO2

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 165.19>99.0%<0.1%<0.2%

<0.02%<0.001%

CAS [63-91-2] 250G1KG

A6226 l-prOliNE High Purity 50GC5H9NO2

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 115.13>98.5%<0.1%<0.3%

<0.02%<0.001%

CAS [147-85-3] 250G1KG

A6227 l-SEriNE High Purity 25GC3H7NO3

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 105.09>98.5%<0.1%<0.2%

<0.02%<0.001%

CAS [56-45-1] 100G500G

A6255 l-tHrEONiNE High Purity 25GC4H9NO3

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 119.12>99.0%<0.1%<0.2%

<0.02%<0.001%

CAS [72-19-5] 100G 500G

A6256 l-tryptOpHAN High Purity 25GC11H12N2O2

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 204.23>98.5%<0.1%<0.3%

<0.03%<0.0015%

CAS [73-22-3] 100G500G

A6253 l-tyrOSiNE High Purity 50GC9H11NO3

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 181.1999.0%<0.1%<0.2%

<0.03%<0.001%

CAS [60-18-4] 250G1KG

A6231 l-VAliNE High Purity 50GC5H11NO2

PurityResidue on IgnitionLoss on DryingSulfateHeavy Metals

MW 117.15>98.5%<0.1%<0.3%

<0.03%<0.0015%

CAS [72-18-4] 250G1KG

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6. Catalog Antibodies

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Catalog Antibodies

Biomatik offers an entire set of popular tag antibodies, loading controls and signal transduction antibodies; our monoclonal antibodies are highly sensitive and specific when compared with similar products from other biotech companies. Caution: All products are for research use only and are not intended for therapeutic or diagnostic applications.Storage: Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot is stable at 4°C for 3 months.

Code product Name Clonality Host Applications Size

CA0001 Anti-His Tag mAb HIS.H8/EH158 Mouse Dot, ELISA, IP, IS, WB 100µg

CA0003 Anti-HA Tag mAb HA.C5/C10 Mouse Dot, ELISA, IP, IS, WB 100µg

CA0005 Anti-Myc Tag Myc.A7/M19 Mouse Dot, ELISA, IP, IS, WB 100µg

CA0006 Anti-GST Tag GST.B6/G2R Mouse Dot, ELISA, IP, IS, WB 100µg

CA0007 Anti-DYKDDDDK (Anti-FLAG) Tag mAb FG4R Mouse Dot, ELISA, IP, IS, WB 100µg

CA0008 Anti-GFP Tag mAb GF28R Mouse Dot, ELISA, IP, IS, WB 100µg

CA0010 Anti-RFP Tag mAb RF5R Mouse Dot, ELISA, IP, IS, WB 100µg

CA0011 Anti-V5 Tag mAb E10/V4RR Mouse Dot, ELISA, IP, IS, WB 100µg

CA1001 Anti-GAPDH Loading Control mAb GA1R Mouse Dot, ELISA, IS, WB 100µg

CA1003 Anti-β-Actin Loading Control mAb BA3R Mouse Dot, ELISA, IS, WB 100µg

CA1007 Anti-ERK1 (E32) Signal Transduction mAb E32 Mouse IP, WB 100µg

CA1008 Anti-ERK1 (E19) Signal Transduction mAb E19 Mouse IP, WB 100µg

CA1009 Anti-ERK1/2 (E31R) Signal Transduction mAb E31R Mouse WB 100µg

Anti-His tagCode-Size CA0001-100µg

description Anti-His Tag Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality HIS.H8/EH158

Host Species Mouse

isotype IgG2b

immunogen HHHHHH (6x His) synthetic peptide conjugated to KLH

Specificity Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag’s location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)

purification Protein A affinity chromatography from mouse ascites fluid

Applications Dot, ELISA, IP, IS, WB

Sensitivity WB (with ECL): 1-2ng of His-tagged fusion proteins

working Conditions

WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution. For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

images

LEFT: untransfected control; MIDDLE: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)RIGHT: standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transected with His-tagged protein vector (2)

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Anti-HA tagCode-Size CA0003-100µg

description Anti-HA Tag Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality HA.C5/C10

Host Species Mouse

isotype IgG3

immunogen YPYDVPDYA (HA) synthetic peptide conjugated to KLH

Specificity Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins

purification Protein A affinity chromatography from mouse ascites fluid

Applications Dot, ELISA, IP, IS, WB

Sensitivity WB (with ECL): 1-5ng of HA-tagged fusion proteins

working Conditions

WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

images

LEFT and MIDDLE: Staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel) RIGHT: 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2)

Anti-myc tagCode-Size CA0005-100µg

description Anti-Myc Tag Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality Myc.A7/M19

Host Species Mouse

isotype IgG1

immunogen EQKLISEEDL (Myc) synthetic peptide conjugated to KLH

Specificity Recognizes the N-terminal or C-terminal Myc-tagged fusion proteins

purification Protein A affinity chromatography from mouse ascites fluid

Applications Dot, ELISA, IP, IS, WB

Sensitivity WB (with ECL): 10-50ng of Myc-tagged fusion proteins

working Conditions

WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

images

TOP: Staining Myc Tag in rat primary hippocampal neurons by ICC/IF. Cells were fixed with paraformaldehyde and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-3% BSA) for 30 minutes at 20°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG (1/500) was used as secondary antibody. BOTTOM: Synapses (red); Anti-Myc tag (green)RIGHT: 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2)

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Anti-GSt tagCode-Size CA0006-100µg

description Anti-GST Tag Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality GST.B6/G2R

Host Species Mouse

isotype IgG2a

immunogen GST protein

Specificity Recognizes native and denatured forms of purified GST or GST fusion proteins

purification Protein A affinity chromatography from mouse ascites fluid

Applications Dot, ELISA, IP, IS, WB

Sensitivity WB (with ECL): 10-50ng of purified GST or GST fusion proteins

working Conditions

WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

images

1:5000 (0.2μg/mL) Ab dilution probed against BL-21 bacteria lysate expressing the GST protein

Anti-dyKddddK (Anti-FlAG) tagCode-Size CA0007-100µg

description Anti-DYKDDDDK (Anti-FLAG) Tag Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality FG4R

Host Species Mouse

isotype IgG2b

immunogen DYKDDDDK (FLAG) synthetic peptide conjugated to KLH

Specificity Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

purification Protein A affinity chromatography from mouse ascites fluid

Applications Dot, ELISA, IP, IS, WB

Sensitivity WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins

working Conditions

WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

images

LEFT: HeLa cells transiently transfected with myc-DDK tagged GFAP ORF cDNA clone and immunostained with Anti-DYKDDDDK (Anti-FLAG) Tag antibody (1:1000 dilution), and then colored in green with Alexa-488 conjugated secondary antibody (1:2000). The nuclei (blue) were counter stained with DAPI. RIGHT: :1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2)

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Anti-GFp tagCode-Size CA0008-100µg

description Anti-GFP (Green Fluorescent Protein) Tag Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality GF28R

Host Species Mouse

isotype IgG1

immunogen GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates

Specificity Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

purification Protein A affinity chromatography from mouse ascites fluid

Applications Dot, ELISA, IP, IS, WB

Sensitivity WB (with ECL): 1-10 ng of purified GFP or GFP fusion proteins

working Conditions

WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

images

LEFT: Cos7 cells transiently transfected with GFP tagged BID ORF cDNA clone for mitochondria marking were immunostained with anti-GFP antibody (1:100), and then stained red with an Alexa-568 conjugated secondary antibody (1:1000)RIGHT: 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used

Anti-rFp tagCode-Size CA0010-100µg

description Anti-RFP (Red Fluorescent Protein) Tag Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality RF5R

Host Species Mouse

isotype IgG1

immunogen RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates

Specificity Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc

purification Protein A affinity chromatography from mouse ascites fluid

Applications Dot, ELISA, IP, IS, WB

Sensitivity WB (with ECL): 1-10ng of purified RFP or RFP fusion proteins

working Conditions

WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

images

1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with RFP-tagged protein vector; untransfected control (1), transfected with Turbo-RFP (2), and transfected with DeRed (3)

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Anti-V5 tagCode-Size CA0011-100µg

description Anti-V5 Tag Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality E10/V4RR

Host Species Mouse

isotype IgG1

immunogen GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH

Specificity Recognizes V5-tagged fusion proteins

purification Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor

Applications Dot, ELISA, IP, IS, WB

Sensitivity WB (with ECL): 1-2ng of V5-tagged fusion proteins

working Conditions

WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

images

1:000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2)

Anti-GApdHCode-Size CA1001-100µgdescription Anti-GAPDH Loading Control Monoclonal AntibodyForm 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azideClonality GA1RHost Species Mouseisotype IgG1immunogen Recombinant GAPDHSpecificity Recognizes native and denatured forms of GAPDH (- 37kDa)Crossreactivity

GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable

purification Protein A affinity chromatography from mouse ascites fluidApplications Dot, ELISA, IS, WB

working Conditions

WB (with ECL): 1:1000-100,000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test

images

LEFT: 1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). RIGHT: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)

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Anti-β-ActinCode-Size CA1003-100µgdescription Anti-β-Actin (BA3R) Loading Control Monoclonal AntibodyForm 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azideClonality BA3RHost Species Mouseisotype IgG2bimmunogen β-Actin N-terminal peptide-KLH conjugatesSpecificity Recognizes native and denatured forms of β-Actin (∼ 42kDa)Crossreactivity

β-Actin from human, mouse, rat, rabbit, and chickenβ-Actin from other species may also be detectable

purification Protein A affinity chromatography from mouse ascites fluidApplications Dot, ELISA, IS, WB

workingConditions

WB (with ECL): 1:1000-3,000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test

images

1:1000 (1μg/mL) Ab dilution used in WB of 20μg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4)

Anti-ErK1 (E32)Code-Size CA1007-100µg

description Anti-ERK1 (E32) Signal Transduction Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality E32

Host Species Mouse

isotype IgG1

immunogen Recombinant full length ERK1 protein

Specificity Recognizes only the ERK1 protein (e.g.: from human and mouse cells)

Crossreactivity

WB: ERK1 from human and mouse cells IP: ERK1 from human cells ERK1 from other species may also be detectable

purification Protein A affinity chromatography from mouse ascites fluid

Applications IP, WBworkingConditions

WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) For best results with other assays (e.g.: IP, etc), please determine optimal working dilution by titration test

images

LEFT: 1:1000 (1μg/mL) Ab dilution used in WB of HEK293 cell lysate; 5μg (1), 10μg (2), and 30μg (3) of cell lysate used; RIGHT: IP of anti-ERK1 (1μL) using HeLa cell lysate; 10μg (1), 25μg (2), and 50μg (3) of cell lysate used

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Anti-ErK1 (E19)Code-Size CA1008-100µg

description Anti-ERK1 (E19) Signal Transduction Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality E19

Host Species Mouse

isotype IgG1

immunogen Recombinant full length ERK1 protein

Specificity Recognizes only the ERK1 protein

Crossreactivity

WB ERK1 from human and mouse cells IP ERK1 from human cells ERK1 from other species may also be detectable

purification Protein A affinity chromatography from mouse ascites fluid

Applications IP, WB (see notations)workingConditions

WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature)For best results with other assays (e.g.: IP, etc), please determine optimal working dilution by titration test

images

LEFT: 1:10,000 (0.1μg/mL) Ab dilution used in WB of HEK293 cell lysate; 1μg (1), 5μg (2), and 10μg (3) of cell lysate used; RIGHT: IP of anti-ERK1 using 50μg of Jurkat cell lysate; 0.5μg (1), 1μg (2), and 2μg (3) of anti-ERK1 used

Anti-ErK1/2 (E31r)Code-Size CA1009-100µg

description Anti-ERK1/2 (E31R) Signal Transduction Monoclonal Antibody

Form 100µg/100µl in 10mM PBS, pH 7.2, with 0.1% sodium azide

Clonality E31R

Host Species Mouse

isotype IgG1

immunogen Full length recombinant human ERK1 protein

Specificity Recognizes human ERK1 and ERK2 proteins

Crossreactivity

WB: ERK1 and ERK2 from human cells ERK1 and ERK2 from other species may also be detectable

purification Protein A affinity chromatography from mouse ascites fluid

Applications WB

workingConditions

WB (with ECL): 1:1000 dilution (incubate for one hour at room temperature) For best results with other assays, please determine optimal working dilution by titration test

images

1:1000 (1μg/mL) Ab dilution used in WB of 10μg (1) and 30μg (2) HeLa cell lysates

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7. Custom Services

7-1 Custom Oligo Synthesis ......................76

7-2 Custom Gene Synthesis ......................77

7-3 Custom Peptide Synthesis ...................78

7-4 Custom Protein Expression ..................80

7-5 Custom Antibody Production ...............81

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Custom Oligo SynthesisWhen it comes to oligonucleotide synthesis, Biomatik has its innovative purification BAP method - Biomatik Affinity Purification. With BAP method, Biomatik can deliver very competitive oligos at high purity level - shorter oligos (<20 bases) could reach as high as 98%. Oligos produced by BAP are suitable for almost any downstream applications.

Standard Oligo SynthesisCode product description 5-9 bases 10-60 bases 61-90 bases 91-110 basesBAP-25 BAP-25nmol, 10-60 bases / 2 OD / /BAP-100 BAP-100nmol, 5-60 bases 2 OD 5 OD / /BAP-200 BAP-200nmol, 5-60 bases 5 OD 10 OD / /

BAP-400 BAP-400nmol, 5-60 bases 10 OD 20 OD / /

BAP-1000 BAP -1µmol, 5-60 bases 20 OD 40 OD / /DNA-5 PAGE (>99%), 100nmol / 2 OD / /DNA-6 PAGE (>99%), 200nmol / 5 OD 2 OD /DNA-7 PAGE (>99%), 1µmol / 20 OD 10 OD 5 ODDNA-8 HPLC (>99%), 100nmol 1 OD 2 OD / /DNA-9 HPLC (>99%), 200nmol 2 OD 5 OD / /DNA-10 HPLC (>99%), 1µmol 10 OD 20 OD / /

Common Oligo modifications3’ Or 5’ FAm 3’ Or 5’ pHOSpHOrylAtiON

FAM-1 PAGE-2OD, 3’ OR 5’ FAM, 100nmol PH-1 PAGE-2OD, 3’ OR 5’ PHOSPHATE, 100nmol

FAM-2 PAGE-5OD, 3’ OR 5’ FAM, 200nmol PH-2 PAGE-5OD, 3’ OR 5’ PHOSPHATE, 200nmol

3’ Or 5’ FitC 3’ Or 5’ AmiNE

FITC-1 PAGE-2OD, 3’ OR 5’ FITC, 100nmol AMINE-1 PAGE-2OD, 3’ OR 5’ AMINO, 100nmol

FITC-2 PAGE-5OD, 3’ OR 5’ FITC, 200nmol AMINE-2 PAGE-5OD, 3’ OR 5’ AMINO, 200nmol

FITC-3 PAGE-10OD, 3’ OR 5’ FITC, 1µmol 3’ Or 5’ tHiOlAtiON

3’ Or 5’ HEX TH-1 PAGE-2OD, 3’ OR 5’ SH C6, 100nmol

HEX-1 PAGE-2OD, 3’ OR 5’ HEX, 100nmol TH-2 PAGE-5OD, 3’ OR 5’ SH C6, 200nmol

HEX-2 PAGE-5OD, 3’ OR 5’ HEX, 200nmol pHOSpHOrOtHiOAtEd

3’ Or 5’ tAmrA PHOS-1 PAGE-2OD, PHOSPHOROTHIOATED, 100nmol

TAMRA-1 PAGE-2OD, 3’ OR 5’ TAMRA, 100nmol PHOS-2 PAGE-5OD, PHOSPHOROTHIOATED, 200nmol

TAMRA-2 PAGE-5OD, 3’ OR 5’ TAMRA, 200nmol du Or dl

3’ Or 5’ rOX DU-1 PAGE-2OD, DU/DI, 100nmol

ROX-1 PAGE-2OD, 3’ OR 5’ ROX, 100nmol DU-2 PAGE-5OD, DU/DI, 200nmol

ROX-2 PAGE-5OD, 3’ OR 5’ ROX, 200nmol 5’ FAm---3’ dabcyl

3’ Or 5’ tEt FD-1 PAGE-2OD, 5’ FAM-3’ DABCYL, 100nmol

TET-1 PAGE-2OD, 3’ OR 5’ TET, 100nmol FD-2 PAGE-5OD, 5’ FAM-3’ DABCYL, 200nmol

TET-2 PAGE-5OD, 3’ OR 5’ TET, 200nmol 5’ FAm---3’ tAmrA

5’ Cy3 Or 5’ Cy5 FT-1 PAGE-2OD, 5’ FAM-3’ TAMRA, 100nmol

CY-1 PAGE-2OD, 5’-CY3 OR CY5, 100nmol FT-2 PAGE-5OD, 5’ FAM-3’ TAMRA, 200nmol

CY-2 PAGE-5OD, 5’-CY3 OR CY5, 200nmol 5’ HEX---3’ tAmrA

3’ dabcyl HT-1 PAGE-2OD, 5’ HEX-3’ TAMRA, 100nmol

DB-1 PAGE-2OD, 5’OR 3’ DABCYL, 100nmol HT-2 PAGE-5OD, 5’ HEX-3’ TAMRA, 200nmol

DB-2 PAGE-5OD, 5’OR 3’ DABCYL, 200nmol 5’ HEX---3’ BHq1

3’ Or 5’ BiOtiN HB-1 PAGE-2OD, 5’ HEX-3’ BHQ1, 100nmol

BIOTIN-1 PAGE-2OD, 3’ OR 5’ BIOTIN, 100nmol HB-2 PAGE-5OD, 5’ HEX-3’ BHQ1, 200nmol

BIOTIN-2 PAGE-5OD, 3’ OR 5’ BIOTIN, 200nmol

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Custom Gene Synthesisdeliver any gene in any vector … more affordable than ever!

Biomatik has been providing highly competitive custom gene synthesis service since year 2004 – the gene synthesis service you can trust and it is affordable! Biomatik particularly specializes in complex genes with high/low GC content and repetitive sequences. Genes up to 1Kb are normally shipped in 2-3 weeks! 

Key Features 100% sequence accuracy guarantee Advanced codon optimization technology Cloning in any vector you desire Rush service in as little as 6 business days

deliverables• 4 µg of lyophilized plasmid containing the gene insert, sequence verified• Complete QC Control data, including QA certificate, project report, construct map, alignment file and sequence chromatograms

Standard Gene Synthesis turnaround time

Gene length Estimated delivery time

≤ 1,000 bp 2 - 3 weeks

1,001 bp - 3, 000 bp 2 - 4 weeks

> 3,000 bp > 4 weeks

rush Gene Synthesis turnaround time (for non-complex genes only)

Gene length Estimated delivery time (Business days)

≤ 800 bp 6 days

801 bp - 1,200 bp 8 days

1,201 bp - 2,000 bp 10 days

Service description of typical Gene Synthesis

1) Free Codon Optimization if requested

2) Synthesis Strategy: sequence analysis and strategy determination

3) Gene Construction: construct the entire gene according to strategy

4) Sub-cloning: clone the synthetic gene into our standard vector or the vector of your choice

5) Sequence Verification: Sequencing the final construct, 100% matching your requested sequence guaranteed

“I’ve been universally happy with your company.”ScientistDepartment of Physics The City College of New York, USA

“The different groups have all found your service very good and this is why another group has decided to go with your company as it has been highly recommended.”ScientistCollege of Life SciencesUniversity of Dundee, UK

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Custom peptide Synthesispeptides at the best quality and prices … only at Biomatik!

Biomatik is the world’s leading supplier of high quality synthetic peptides for academia and industry.  With flexible scale ranging from milligrams

to kilograms, wide range of labeling and modification services, and high-throughput peptide library synthesis capacity, we are able to synthesize

almost all kinds of peptides that have appeared in the literature, in the shortest possible time frame. Each peptide synthesis is completed under

stringent quality control; most purified peptides under 30aa are delivered in 2-3 weeks!

Key Features

High success rate

For peptides up to 130aa

From milligrams to kilograms

Crude to 99+% by HPLC

Comprehensive labeling and modifications

Extensive experience in lengthy and complex peptides

Rush service in as little as 7 business days

deliverables

• Peptide in lyophilized powder

• Certificate of Analysis, QC Data (HPLC & MS)

peptide Synthesis price reference table (partially listed only)

qty/purity Crude 75% 85% 98%

4 mg $4.10 $9.20 $11.90 $22.23

19 mg $6.24 $13.70 $19.09 $34.75

49 mg $8.51 $19.56 $25.23 $48.77

100 mg $10.87 $30.90 $37.04 $69.24

1000 mg $38.93 $92.89 $111.04 $207.00

Price per residue, valid for peptides less than 30aa only; Minimum $100 per peptide.

“Over the years, I have used several companies to synthesize peptides for my research. My experience with Biomatik has been top notch. Biomatik offers quality products at competitive prices. In fact, the latest peptide I ordered from them was about half the cost of several competitors. Their customer service personnel are competent and professional, making the process of ordering peptides quick and pain-free. I will continue to use Biomatik for all my peptide synthesis needs.”

ScientistDept. of Cell & Tissue BiologyUniversity of California, San FranciscoSan Francisco, CA 94143, USA

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Common peptide labeling and modificationsN-terminal modifications C-terminal modificationsAcetylation (N-Terminal) -NHCOCH3 Amidation (C-Terminal) -CONH2

Biotin (N-Terminal) AMC (C-Terminal)

Biotin-Ahx (N-Terminal) Alcohol (C-Terminal)

Abz (N-Terminal) Bzl (C-Terminal)

Cbz (Benzyloxycarbonylation) (N-Terminal) Cysteamide (C-Terminal)

Dansyl (N-Terminal) Ester (OEt) (C-Terminal)

Dansyl-Ahx (N-Terminal) Ester (OMe) (C-Terminal)

DTPA (N-Terminal) Et (C-Terminal)

5-FAM (N-Terminal) Biotin (Lys side chain, C-Terminal)

5-FAM-Ahx (N-Terminal) Dinitrobenzylation (Lys side chain, C-Terminal)

FITC-Ahx (N-Terminal) FITC (Lys side chain, C-Terminal)

TAMRA (N-Terminal) 5-FAM (Lys side chain, C-Terminal)

Fatty Acid (N-Terminal) TAMRA (Lys side chain, C-Terminal)

Fmoc (N-Terminal) Me (C-Terminal)

Formylation (N-Terminal) NHEt (C-Terminal)

His-Tag (N-Terminal) NHMe (C-Terminal)

HYNIC (N-Terminal) OSu (C-Terminal)

MCA (N-Terminal) tBu (C-Terminal)

Myristoyl (N-Terminal) TBzl (C-Terminal)

Mini-PEG (N-Terminal) MAPS Asymmetric 4 branches (C-Terminal)

Palmitoyl (N-Terminal) MAPS Asymmetric 8 branches (C-Terminal)

Suc (Succinylation) (N-Terminal)

Standard modifications ConjugationsAbz/DNP KLH Conjugation on cysteine

DABCYL BSA Conjugation on cysteine

DABCYL/Glu(EDANS)-NH2 OVA Conjugation

EDANS/DABCYL KLH (-NH2 of N terminal)

Glu(EDANS)-NH2 BSA (-COOH of C terminal)

MCA/DNP KLH (-COOH of C terminal)

mini-PEG BSA (-NH2 of N terminal)

pNA (p-Nitroanilide)

Succinylation

Amide cyclic (side chain) related ServicesAmide cyclic (Head to Tail) Amino Acid Analysis

Disulfide Bridge Peptide Content Analysis (nitrogen estimation)

Disulfide Bridge, x2 TFA Salt Content Analysis

Disulfide Bridge, x3 Aliquoting (free for up to 5 vials)

“Easy-to-obtain prompt quotes online, reasonable prices, very responsive service, and high quality peptides. I will continue to use Biomatik and recommend them to colleagues.”

ScientistHead, High-Throughput Screening & Drug Discovery LabNemours Center for Childhood Cancer ResearchWilmington DE 19803, USA

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Custom protein ExpressionCustom protein Service that is dependable and Affordable!

Biomatik’s proprietary protein expression and purification platforms can produce high quality functional recombinant proteins used in biomedical

and pharmaceutical research. Given our extensive expertise in gene synthesis service, we can start your recombinant protein project from

anywhere you wish! It can be from de novo gene synthesis, or from customer provided materials (plasmids or expression strains) to protein

expression, and to final refolding and purification, and deliver high quality purified protein in a timely and cost effective manner. Moreover, our

committed technical specialists will guide you through every step of the project, and keep you posted of the project progress.

Custom protein Service packages

There are four recombinant protein expression systems, including bacteria, yeast, baculovirus infected insect cells, and mammalian cells. E. coli

protein expression system has several advantages, including ease of culture, rapid cell growth and relatively simple purification procedures. We

list our popular E. coli protein expression and purification packages below. Feel free to inquire if you are interested in other expression systems.

protein Expression package description (E. coli)

#pE1001 5mg E. coli Guaranteed Protein Package Service Includes: Subcloning, Expression optimization & verification, and Expression (with tag) & purification

deliverables:Guaranteed 5mg purified soluble protein from E. coli; QC data

#pE1002 15mg E. coli Guaranteed Protein Package Service Includes: Subcloning, Expression optimization & verification, and Expression (with tag) & purification

deliverables:Guaranteed 15mg purified soluble protein from E. coli; QC data

#pE1003 5mg E. coli Guaranteed Protein Package (with tag removal) Service Includes: Subcloning, Expression (with tag) optimization & verification, Expression & purification, tag removal

deliverables:Guaranteed 5mg purified soluble protein from E. coli, tag removed; QC data

#pE1004 15mg E. coli Guaranteed Protein Package (with tag removal) Service Includes: Subcloning, Expression (with tag) optimization & verification, Expression & purification, tag removal

deliverables:Guaranteed 15mg purified soluble protein from E. coli, tag removed; QC data

1. Customer to provide the plasmid or the expression strain. If there is no material to provide, Biomatik can start with gene synthesis.

2. Size of the target protein is usually less than 100KDa; Price is subject to change if it is greater than 100KDa.

3. QC data includes SDS-PAGE & Western Blot (available only for HIS tagged proteins). For other tagged proteins or if HIS tag has been

removed, additional charges may apply for WB detection if required.

4. Biomatik guarantees protein to be soluble, but not the bioactivity or functionality. If target protein expresses as inclusion body, refolding will be done to make it soluble.

5. Biomatik does not guarantee the endotoxin level of purified protein; Endotoxin removal can be requested at additional cost.

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Custom Antibody production

Biomatik has set higher standards for its custom polyclonal and monoclonal antibody production services in the industry with ELISA and application

guaranteed antibodies. With our gene synthesis, peptide synthesis and protein expression services, we can make any antibody from scratch, at

very affordable prices. From cDNA, plasmid, expression strain, peptide, protein …whatever you wish to start with for your antibody needs,

Biomatik will find a route to custom make the antibody you desire!

Custom polyclonal Antibody packages

Code#SA0033 peptide polyclonal Antibody package>Peptide antigen, 2 rabbits>From peptide synthesis and conjugation, immunization to antigen affinity purified antibody.

Guarantee: ELISA >1:32,000EDT: 3~4 months

deliverables1) 3-5 mg affinity purified antibody2) 0.5 ml pre-bleed each rabbit3) 5 mg peptide4) Project report with QC data

Code#SA0031 protein polyclonal Antibody package>Protein antigen, 2 rabbits>Customer to provide protein>From immunization to protein A/G purified antibody

Guarantee: ELISA >1:32,000EDT: ~3 months

deliverables1) 15-25 mg protein A/G purified antibody2) 0.5 ml pre-bleed each rabbit3) Project report with QC data

Code#SA0035 phospho-peptide polyclonal Antibody package>Phospho specific peptide antigen, 2 rabbits >From peptide synthesis and conjugation, immunization, to antigen affinity purified antibody

Guarantee: ELISA >1:32,000, dot blot positiveEDT: 4~5 months

deliverables1) 2-5 mg phospho-specific antibody2) 3-5 mg non-phospho-specific antibody3) 0.5 ml pre-bleed each rabbit4) phospho-peptide and non-phospho peptide, 2-3 mg each5) Project report with QC data

Code#SA0050 protein polyclonal Antibody package>Protein antigen, 2 rabbits >Customer to provide plasmid or the expression strain >From subcloning, protein expression and purification, immunization, to protein A/G purified antibody

Guarantee: ELISA >1:32,000EDT: ~4 months

deliverables1) 15-25 mg protein A/G purified antibody2) 0.5 ml pre-bleed each rabbit3) 1 mg protein4) Project report with QC data

Code#SA0051 protein polyclonal Antibody package>Protein antigen, 2 rabbits >Customer to provide cDNA >From vector construction, subcloning, protein expression and purification, immunization, to protein A/G purified antibody

Guarantee: ELISA >1:32,000EDT: 4~5 months

deliverables1) 15-25 mg protein A/G purified antibody2) 0.5 ml pre-bleed each rabbit3) 1 mg protein4) Project report with QC data

Code#SA0052 protein polyclonal Antibody package>Protein antigen, 2 rabbits >From gene synthesis, subcloning, protein expression and purification, immunization, to protein A/G purified antibody

Guarantee: ELISA >1:32,000EDT: 4~5 months

deliverables1) 15-25 mg protein A/G purified antibody2) 0.5 ml pre-bleed each rabbit3) 1 mg protein4) 4 µg plasmid5) Project report with QC data

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Custom monoclonal Antibody packages

Code#SA0131 peptide monoclonal Antibody package>Peptide antigen, 3-5 Balb/c mice>From peptide synthesis and conjugation, immunization, ELISA identification, fusion and hybridoma development, isotype determination, subcloning and cell cryopreservation, to ascites production and protein A/G purified antibody

Guarantee: ELISA >1:80,000EDT: 6~7 months

deliverables1) 2-5 clones2) 2-5 mg purified antibodies (2 clones)3) 50 µl pre-immune sera (1:10 dilution)4) 2-3 mg peptide5) Project report with QC data

Code#SA0132 protein monoclonal Antibody package>Protein antigen, 3-5 Balb/c mice>Customer to provide protein>From immunization, ELISA identification, fusion and hybridoma development, isotype determination, subcloning and cell cryopreservation, to ascites production and protein A/G purified antibody

Guarantee: ELISA >1:80,000EDT: ~6 months

deliverables1) 2-5 clones2) 2-5 mg purified antibodies (2 clones)3) 50 µl pre-immune sera (1:10 dilution)4) Project report with QC data

Code#SA0133 phospho-peptide monoclonal Antibody package>Phospho specific peptide antigen, 3-5 Balb/c mice >From peptide synthesis and conjugation, immunization, ELISA identification, fusion and hybridoma development, isotype determination, subcloning and cell cryopreservation, to ascites production and protein A/G purified antibody

Guarantee: ELISA (Ab titer) >1:80,000; ELISA (phospho/unmodified) >100EDT: ~7 months

deliverables1) 2-5 clones2) 2-5 mg purified antibodies (2 clones)3) 50 µl pre-immune sera (1:10 dilution)4) phospho-peptide and non-phospho peptide, 2-3 mg each5) Project report with QC data

Code#SA0150 protein monoclonal Antibody package>Protein antigen, 3-5 Balb/c mice >Customer to provide plasmid or the expression strain >From subcloning, protein expression and purification, immunization, ELISA identification, fusion and hybridoma development, isotype determination, subcloning and cell cryopreservation, to ascites production and protein A/G purified antibody

Guarantee: ELISA >1:80,000EDT: ~8 months

deliverables1) 2-5 clones2) 2-5 mg purified antibodies (2 clones)3) 50 µl pre-immune sera (1:10 dilution)4) 1 mg protein5) Project report with QC data

Code#SA0151 protein monoclonal Antibody package>Protein antigen, 3-5 Balb/c mice>Customer to provide cDNA >From vector construction, subcloning, protein expression and purification, immunization, ELISA identification, fusion and hybridoma development, isotype determination, subcloning and cell cryopreservation, to ascites production and protein A/G purified antibody

Guarantee: ELISA >1:80,000EDT: ~8 months

deliverables1) 2-5 clones2) 2-5 mg purified antibodies (2 clones)3) 50 µl pre-immune sera (1:10 dilution)4) 1 mg protein5) Project report with QC data

Code#SA0152 protein monoclonal Antibody package>Protein antigen, 3-5 Balb/c mice >From gene synthesis, subcloning, protein expression and purification, immunization, ELISA identification, fusion and hybridoma development, isotype determination, subcloning and cell cryopreservation, to ascites production and protein A/G purified antibody

Guarantee: ELISA >1:80,000EDT: ~8 months

deliverables1) 2-5 clones2) 2-5 mg purified antibodies (2 clones)3) 50 µl pre-immune sera (1:10 dilution)4) 1 mg protein5) 4 µg plasmid6) Project report with QC data

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8. Appendix - Technical Reference Data

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8Commonly Used Material Grades

ACS Grade Materials conforming with the specifications and procedures outlined in American Chemical Society Specifi-cations, 10th Edition, 2006.

Ultra Pure Grade Material with a purity level exceeding the various monograph grades.

USP Grade Materials conforming with the specifications and procedures outlined in the United States Pharmacopeia, 31st Edition, 2008.

Biotechnology Grade /Molecular Biology Grade

Materials equivalent to Ultra Pure, but particularly suitable for use in Molecular Biology applications. Tested for specific contaminants such as nucleases and bacteria where appropriate.

Electrophoresis Grade Material specifically for Electrophoretic Applications.

High Purity Grade Materials of superior quality where there are no published standards.

Reagent Grade High Purity materials which meet the specifications noted in our catalog.

Technical Grade Designates materials of selected commercial or industrial quality.

Tissue Culture Grade Materials of high purity, suitable for use in Tissue Culture applications.

Analytical Grade Designates reagents suitable for use in analytical procedures.

Certified/Certifiable Grade Materials, typically dyes and stains that meet the requirements of the Biological Stain Commission. Certified reagents have been tested and validated by the Biological Stain Commission.

Conjugation Grade Material suitable for enzyme conjugation. 

 Measurement Conversions Weight 1 milligram =0.015 grain 1 centigram =10 mg =0.154 grain 1 decigram =10 cg =1.543 grains

1 gram =10 dg =15.43 grains 1 decagram =10 g =5.64 drams 1 hectogram =100 g =3.527 ounces

1 kilogram =1000 g =2.205 pounds 1 ton (metric ton) =1000 kg =0.984 (long) ton =1.1023

Capacity Measure1 millilitre =0.002 pint 1 centilitre =10 ml =0.018 pint 1 decilitre =10 cl =0.176 pint

1 litre =10 dl =1.76 pints 1 decalitre =10 l =2.20 gallons

1 hectolitre =100 l =2.75 bushels 1 kilolitre =1000 l =3.44 quarters

 Capacity Measure – British Capacity Measure - American dry Capacity Measure - American liquid1 pint =20 fluid oz =34.68 cu.in. =0.568 litre 1 quart =2 pints =1.136 litres 1 gallon =4 quarts =4.546 litres 1 peck =2 gallons =9.092 litres 1 bushel =4 pecks =36.4 litres 1 quarter =8 bushels=2.91 hectolitres

1 pint =33.60 cu.in. =0.550 litre 1 quart =2 pints =1.101 litres 1 peck =8 quarts =8.81 litres 1 bushel =4 pecks =35.3 litres

 

1 pint =16 fluid oz =28.88 cu.in. =0.473 litre 1 quart =2 pints =0.946 litre 1 gallon =4 quarts =3.785 litres

Linear Measure 1 inch =25.4 millimetres 1 foot =12 inches =0.3048 metre 1 yard =3 feet =0.9144 metre 1 (statute) mile =1760 yards =1.609 kilometres 1 nautical mile =1852 m

1 millimetre =0.03937 inch 1 centimetre =10 mm. =0.3937 inch 1 decimetre =10 cm. =3.937 inches 1 metre =10 dm =1.0936 yards =3.2808 feet 1 decametre =10 m =10.936 yards 1 hectometre =100 m =109.4 yards 1 kilometre =1000 m =0.6214 mile 1 mile marin =1852 m =1.1500 mile

Square Measure 1 square inch =6.45 sq.centimetres 1 square foot =144 sq.in.=9.29 sq.decimetres 1 square yard =9 sq.ft.=0.836 sq.metre 1 acre =4840 sq.yd. =0.405 hectare 1 square mile =640 acres =259 hectares

1 square centimetre =0.155 sq.inch 1 square metre =1.196 sq.yards 1 are =100 square metres =119.6 sq.yards 1 hectare =100 ares =2.471 acres 1 square kilometre =0.386 sq.mile

Cubic Measure 1 cubic inch =16.4 cu.centimetres 1 cubic foot =1728 cu.in.=0.0283 cu.metre 1 cubic yard =27 cu.ft.=0.765 cu.metr

1 cubic centimetre =0.061 cu.inch 1 cubic metre =1.308 cu.yards

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Bulk quantities and custom formulations available upon request

527

Commonly Used Abbreviations

A A adenine or adenosine; one-letter code for alanine A260 absorbance at 260 nm ACES N-(2-acetamido)-2-aminoethanesulfonic acid Ad-2 adenovirus-2 ADA N-(2-acetamido)-2-iminodiacetic acid ADP adenosine 5’-diphosphate AMP adenosine monophosphate AMV Avian Myeloblastosis Virus AP alkaline phosphatase APS ammonium persulfate aRNA antisense RNA ATP adenosine 5’-triphosphate B BCIP 5-bromo-4-chloro-3-indolyl phosphate BES N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid BGG bovine gamma globulin BICINE N,N-bis(2-hydroxyethyl)glycine Bio-dNTP biotin-deoxynucleoside triphosphate BME -mercaptoethanol; 2-mercaptoethanol bp base pair Bq Becquerel BSA bovine serum albumin B/W blue/white cloning C C cytosine or cytidine; one-letter code for cysteine CA casamino-acids CAPS N-cyclohexyl-3-aminopropanesulfonic acid cDNA complementary deoxyribonucleic acid CEU cohesive wnd ligation unit Ci Curie CIAP calf intestinal alkaline phosphatase CMP cytidine monophosphate cpm counts per minute CTP cytidine 5’-triphosphate D Da Dalton dAMP deoxyadenosine monophosphate dATP deoxyadenosine triphosphate dCTP deoxycytidine triphosphate ddATP dideoxyadenosine triphosphate ddCTP dideoxycytidine triphosphate ddGTP dideoxyguanosine triphosphate ddNTP dideoxynucleoside triphosphate ddTTP dideoxythymidine triphosphate DE-81 Whatman® diethylaminoethyl cellulose paper DEPC diethyl pyrocarbonate DIG digoxigenin dITP deoxyinosine triphosphate dGTP deoxyguanosine triphosphate DMSO dimethyl sulfoxide DNA deoxyribonucleic acid DNase deoxyribonuclease dNMP deoxyribonucleoside monophosphate dNTP deoxynucleoside triphosphate dpm disintegrations per minute ds double-stranded DTT dithiothreitol dTTP deoxythymidine triphosphate dUTP deoxyuridine triphosphate E EDTA ethylenediaminetetraacetic acid EGTA ethylene glycol tetraacetic acid ELISA enzyme-linked immunosorbent assay EMBL European Molecular Biology Laboratory EMSA electrophoretic mobility shift assay Endo endodeoxyribonuclease assay endo endonuclease

exo exonuclease Exo 5’ and 3’- exodeoxyribonuclease assay F FastAP™ FastAP™ thermosensitive alkaline phosphatase FDU fast didest unit FOA (5-FOA) 5-fluoroorotic acid G G guanine or guanosine; one-letter code for glycine Gal D-galactose GMP guanosine monophosphate GMPD glycosylase mediated polymorphism detection GUS -D-glucuronidase GTP guanosine 5’-triphosphate GQ genetic quality H HC high concentration HEPES N-(2-hydroxyethyl)piperazine-N’-(2-ethanesulfonic acid) HRP horseradish peroxidase HPLC high-performance liquid chromatography I IEF isoelectric focussing IPTG isopropyl- -D-thiogalactopyranoside K kb kilobase kDa kiloDalton L LAMP loop-mediated isothermal amplification LB Luria Bertani media LC low concentration LE low electroendosmosis LM low melting point LO labeled oligonucleotide M MCS multiple cloning site MES 2-(N-morpholino)ethanesulfonic acid MDA multiple displacement amplification M-MuLV Moloney Murine Leukemia Virus MOPS 3-(N-morpholino)propanesulfonic acid mRNA messenger ribonucleic acid MW molecular weight N NAD nicotinamide adenine dinucleotide NADH nicotinamide adenine dinucleotide, reduced form NADP nicotinamide adenine dinucleotide phosphate NADPH nicotinamide adenine dinucleotide phosphate, reduced form NBT nitro blue tetrazolium NDP nucleoside diphosphate NMP ribonucleoside monophosphate NR not recommended NP-40 Nonidet P-40 (detergent) nt nucleotide NTC no-template control NTP nucleoside triphosphate O oligo(A) oligoadenylic acid oligo(dT) oligodeoxythymidylic acid OMP orotidine monophosphate P Pi inorganic phosphate PAAG polyacrylamide gel PAGE polyacrylamide-gel electrophoresis PBS phosphate-buffered saline PCR polymerase chain reaction PEI polyethylenimine PEG polyethylene glycol PIPES piperazine-N,N’-bis(2-ethanesulfonic acid) PNK polynucleotide kinase pNPP 4-nitrophenyl phosphate; para-nitrophenyl phosphate poly(A) polyadenylic acid poly(A)+ polyadenylated (mRNA) poly(dA-dT) poly (deoxyadenylic acid – deoxythymidylic acid)

(continued on next page)

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www.fermentas.com524

For patent and license information see p.557

100X Denhardt Solution: Final concentration:Ficoll 400 10 g 0.02% (w/v)Polyvinylpyrrolidone 10 g 0.02% (w/v)Bovine serum albumin 10 g 0.02% (w/v)H2O to 500 ml

Filter sterilize and store at -20°C in 25 ml aliquots1 M Dithiothreitol (DTT):

DTT 15.45 g H2O to100 ml

Store at -20°C0.5 M Ethylenediamine Tetraacetic Acid (EDTA) (pH 8.0):

Na2EDTAx2H2O 186.1 g H2O to 700 ml

Adjust pH to 8.0 with 10 M NaOH (~50 ml)H2O to 1 liter

10 mg/ml Ethidium Bromide:Ethidium bromide 0.2 g H2O to 20 ml

Mix well and store at 4°C in dark.CAUTION: Ethidium bromide is a mutagen. Wear gloves when working with solutions and a mask when weighing the powder.1 M KCl:

KCl 74.6 gH2O to 1 liter

1 M MgCl2:MgCl2x6H2O 20.3 gH2O to 100 ml

1 M MgSO4:MgSO4x7H2O 24.6gH2O to 100ml

5M NaCl:NaCl 292.2 gH2O to 1 liter

10 M NaOH:NaOH 400.0 g H2O to 1 liter

1 M Tris-HCl [tris(hydroxymethyl)aminomethane]:Tris base 121.1 gH2O to 800 ml

Adjust to desired pH with concentrated HCl.Mix and add H2O to 1 liter3 M Sodium Acetate (pH 5.2 or 7.0) (2):

Sodium acetate x 3H2O 408.1 gH2O to 800 ml

Adjust the pH to 7.0 with acetic acid or adjust the pH to 5.2 with glacial acetic acid.H2O to 1 liter

Buffers (2)

10X Stock Phosphate-buffered Saline (PBS), per liter: Final 1X concentration:NaCl 80.0 g 137 mMKCl 2.0 g 2.7 mMNa2HPO4 14.4 g 10 mMKH2PO4 2.7 g 2 mMH2O to 800 ml

Adjust to pH 7.4 with concentrated HCl.H2O to 1 liter

20X SSC, per liter: Final 1X concentration:NaCl 175.3 g 150 mMNa3citrate x 2H2O 88.2 g 15 mMH2O to 800 ml

Adjust pH to 7.0 with 1M HClH2O to 1 liter

Growth Media (1)LB Medium, per liter: Final 1X concentration:

Tryptone 10 g 1.0% (w/v)Yeast extract 5 g 0.5% (w/v)NaCl 10 g 1.0% (w/v)H2O to 1 liter

Adjust pH to 7.0.Low Salt LB Medium, per liter: Final 1X concentration:

Tryptone 10 g 1.0% (w/v)Yeast extract 5 g 0.5% (w/v)NaCl 5 g 0.5% (w/v)H2O to 1 liter

Adjust pH to 7.0.Terrific Broth Medium, per liter: Final 1X concentration:

Tryptone 12 g 1.2% (w/v)Yeast extract 24 g 2.4% (w/v)Glycerol 4 ml 0.4% (w/v)H2O to 900 ml

Autoclave, cool to 60°C or less before adding 100 ml of filter sterilized 10X TB phosphate (0.17 M KH2PO4, 0.72 M K2HPO4).SOB Medium, per liter Final 1X concentration:

Tryptone 20 g 2.0% (w/v)Yeast extract 5 g 0.5% (w/v)NaCl 0.5 g 0.05% (w/v)250 mM KCl 10 ml 2.5 mMH2O to 900 ml

Adjust pH to 7.0 and add H2O to 990 ml.Autoclave, cool to room temperature and just prior to use add 10 ml 1 M MgCl2 (sterile solution). 10 mMSOC Medium, per liter:

SOB Medium (1 liter) with the addition of 20 ml filter sterilized 1 M glucose.

M9 Minimal Medium, per liter: Final 1X concentration:5X M9 salts 200 mlSterile H2O to 1 liter1 M MgSO4 1 ml 1 mM20% glucose 10 ml 0.2% (w/v)1M CaCl2 0.1 ml 0.1 mM

5X M9 Salts, per liter: Final 1X concentration:Na2HPO4x7H2O 64 g 47.8 mMKH2PO4 15 g 22 mMNaCl 2.5 g 8.6 mMNH4Cl 5 g 18.7 mM

Additives Final 1X concentration:Antibiotics (if required):

Ampicillin 50 μg/mlChloramphenicol 20 μg/mlKanamycin 30 μg/mlTetracycline 12 μg/ml

Galactosides (if required):X-Gal 20 μg/mlIPTG 0.1 mM

Media containing agar or agarose, per liter:Agar (for plates) 15 g 1.5% (w/v)Agar (for top agar) 7 g 0.7% (w/v)Agarose (for plates) 15 g 1.5% (w/v)Agarose (for top agarose) 7 g 0.7% (w/v)

Stock Solutions10 M Ammonium Acetate:

Ammonium acetate 385.4 g H2O to 500 ml

1 M CaCl2:CaCl2x6H2O 219.1 gH2O to 1 liter

Commonly Used Media, Stock Solutions and Buffers

(continued on next page)

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20X SSPE, per liter: Final 1X concentration:NaCl 175.3 g 150 mMNaH2PO4xH2O 27.6 g 10 mMNa2EDTA 7.4 g 1 mMH2O to 800 ml

Adjust pH to 7.4 with 10 M NaOHH2O to 1 liter

5X Tris-glycine, Native Electrophoresis Buffer, per liter: Final 1X concentration:Tris base 15.1 g 25 mM Glycine 72.0 g 192 mMH2O to 1 liter

The pH of diluted solution is pH 8.3.5X Tris-glycine-SDS Electrophoresis Buffer, per liter: Final 1X concentration:

Tris base 15.1 g 25 mM Glycine 72.0 g 192 mMSDS 5.0 g 0.1% (w/v)H2O to 1 liter

The pH of diluted solution is pH 8.3.5X Tris-tricine-SDS Electrophoresis Buffer, per liter: Final 1X concentration:

Tris base 60.6 g 0.1 M Tricine 89.6 g 0.1 MSDS 10.0 g 0.1% (w/v)H2O to 1 liter

The pH of diluted solution is pH 8.3.50X TAE (Tris-acetate-EDTA) Electrophoresis Buffer, per liter: Final 1X concentration:

Tris base 242 g 40 mMGlacial acetic acid 57.1 ml 20 mM0.5 M EDTA (pH 8.0) 100 ml 1 mMH2O to 1 liter

The pH of diluted solution is ~8.510X TBE (Tris-borate-EDTA) Electrophoresis Buffer, per liter: Final 1X concentration:

Tris base 108 g 89 mMBoric acid 55 g 89 mM0.5 M EDTA (pH 8.0) 40 ml 2 mMH2O to 1 liter

10X TPE (Tris-phosphate-EDTA) Electrophoresis Buffer, per liter: Final 1X concentration:Tris base 108 g 89 mMPhosphoric acid (85%) 15.5 ml 27 mM0.5 M EDTA (pH 8.0) 40 ml 2 mMH2O to 1 liter

TE (Tris-EDTA) Buffer, pH 7.4, 7.6 or 8.0, per liter: Final 1X concentration:1 M Tris, pH 7.4, 7.6, 8.0 10 ml 10 mM0.5 M EDTA (pH 8.0) 2 ml 1 mMH2O to 1 liter

References 1. Atlas, R.M., Handbook of Microbiological Media, second edition, Ed. Parks, L.C., CRC

Press, N.Y., 1997.2. Sambrook, J., et al., Molecular Cloning: A Laboratory Manual, second edition, Cold Spring

Harbor Laboratory Press, Cold Spring Harbor, NY, A1.2-A2.12, 2001.

Expressing Concentrations of Reagents

Molarity number of moles solute M (mol/l) = number of liters of solution

Percent composition weight of solute (g) Concentration (%) = x 100 total weight of solution (g)

Percent composition (weight/volume) weight of solute (g) Concentration (% w/v) = x 100 total weight of solution (ml)

Percent composition (volume/volume) weight of solute (ml) Concentration (% v/v) = x 100 total weight of solution (ml)

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Temperature Dependence of the pH for Commonly Used Buffers

Buffer system Chemical name pKa/20°C pKa/10°CMES 4-morpholineethanesulfonic acid 6.15 -0.110

ADA [(carbamoylmethyl)imino]diacetic acid 6.60 -0.110

PIPES 1,4-piperazinediethanesulfonic acid 6.80 -0.085

ACES 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid 6.90 -0.200

BES 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid 7.15 -0.160

MOPS 4-morpholinepropanesulfonic acid 7.20 -0.013

TES 2-{[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino}-1-propanesulfonic acid

7.50 -0.200

HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 7.55 -0.140

TRICINE N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine 8.15 -0.210

TRIS 2-amino-2-hydroxymethylpropane-1,3-diol 8.30 -0.310

BICINE N,N-bis(2-hydroxyethyl)glycine 8.35 -0.180

GLYCYLGLYCINE 2-(2-aminoacetyl)aminoacetic acid 8.40 -0.280

Decay Factors for Calculating the Amount of Radioactivity32P, (half-life – 14.3 days)

Days Hours0 12 24 36 48 60 72 84

0 1.000 0.976 0.953 0.930 0.908 0.886 0.865 0.8444 0.824 0.804 0.785 0.766 0.748 0.730 0.712 0.6958 0.679 0.662 0.646 0.631 0.616 0.601 0.587 0.57312 0.559 0.546 0.533 0.520 0.507 0.495 0.483 0.47216 0.460 0.449 0.439 0.428 0.418 0.408 0.398 0.38920 0.379 0.370 0.361 0.353 0.344 0.336 0.328 0.32024 0.312 0.305 0.298 0.291 0.284 0.277 0.270 0.26428 0.257 0.251 0.245 0.239 0.234 0.228 0.223 0.21732 0.212 0.207 0.202 0.197 0.192 0.188 0.183 0.17936 0.175 0.170 0.166 0.162 0.159 0.155 0.151 0.14740 0.144 0.140 0.137 0.134 0.131 0.127 0.124 0.12144 0.119 0.116 0.113 0.110 0.108 0.105 0.102 0.10048 0.098 0.095 0.093 0.091 0.089 0.086 0.084 0.08252 0.080 0.078 0.077 0.075 0.073 0.071 0.070 0.068

35S, (half-life – 84.4 days)

Weeks Days0 1 2 3 4 5 6

0 1.000 0.992 0.984 0.976 0.969 0.961 0.9541 0.946 0.939 0.931 0.924 0.916 0.909 0.9022 0.895 0.888 0.881 0.874 0.867 0.860 0.8533 0.847 0.840 0.833 0.827 0.820 0.814 0.8074 0.801 0.795 0.788 0.782 0.776 0.770 0.7645 0.758 0.752 0.746 0.740 0.734 0.728 0.7226 0.717 0.711 0.705 0.700 0.694 0.689 0.6837 0.678 0.673 0.667 0.662 0.657 0.652 0.6468 0.641 0.636 0.631 0.626 0.621 0.616 0.6129 0.607 0.602 0.597 0.592 0.588 0.583 0.57910 0.574 0.569 0.565 0.560 0.556 0.552 0.54711 0.543 0.539 0.534 0.530 0.526 0.522 0.51812 0.514 0.510 0.506 0.502 0.498 0.494 0.490

33P, (half-life – 25.4 days)

Days Days0 1 1 3 4 5 6 7 8 8

0 1.000 0.973 0.947 0.921 0.897 0.872 0.849 0.826 0.804 0.78210 0.761 0.741 0.721 0.701 0.683 0.664 0.646 0.629 0.612 0.59520 0.579 0.564 0.549 0.534 0.520 0.506 0.492 0.479 0.466 0.45330 0.441 0.429 0.418 0.406 0.395 0.385 0.374 0.364 0.355 0.34540 0.336 0.327 0.318 0.309 0.301 0.293 0.285 0.277 0.270 0.26350 0.256 0.249 0.242 0.236 0.229 0.223 0.217 0.211 0.205 0.20060 0.195 0.189 0.184 0.179 0.174 0.170 0.165 0.161 0.156 0.15270 0.148 0.144 0.140 0.136 0.133 0.129 0.126 0.122 0.119 0.11680 0.113 0.110 0.107 0.104 0.101 0.098 0.096 0.093 0.091 0.08890 0.086 0.084 0.081 0.079 0.077 0.075 0.073 0.071 0.069 0.067100 0.065 0.064 0.062 0.060 0.059 0.057 0.055 0.054 0.053 0.051110 0.050 0.048 0.047 0.046 0.045 0.043 0.042 0.041 0.040 0.039120 0.038 0.037 0.036 0.035 0.034 0.033 0.032 0.031 0.030 0.030

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DNA Migration in Agarose and Polyacrylamide Gels

Reference 1. Sambrook, J., et al., Molecular Cloning. A Laboratory

Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 12.89, 5.42, 2001.

Recommended agarose gels for electrophoretic separation of DNA fragments.

Agarose gel, %

Range of effective

separation, bp

Approximate positions of tracking dyes, bp*

Bromophenol blue Xylene cyanol FF

TBE buffer TAE buffer TBE buffer TAE buffer0.5 2000-50000 750 1150 13000 167000.6 1000-20000 540 850 8820 116000.7 800-12000 410 660 6400 85000.8 800-10000 320 530 4830 65000.9 600-10000 260 440 3770 51401.0 400-8000 220 370 3030 41601.2 300-7000 160 275 2070 28901.5 200-3000 110 190 1300 18402.0 100-2000 65 120 710 10403.0 25-1000 30 60 300 4604.0 10-500 18 40 170 2605.0 10-300 12 27 105 165

* Positions of the tracking dyes can be estimated only approximately because the dye front migrates as fuzzy band. To use the data above, the following guidelines are recommended:

• Agarose should be top quality. The Fermentas TopVision™ LE GQ Agarose (#R0491) was used to prepare the gels.

• Only freshly prepared electrophoresis buffers should be used. The buffers were prepared from the stocks supplied by Fermentas (50X TAE Buffer (#B49) and 10X TBE Buffer (#B52), respectively).

Recommended polyacrylamide gels for electrophoretic separation of DNA fragments (1).

Polyacrylamide gel (with BIS at 1:20), % (w/v)

Range of effective

separation*

Approximate positions of tracking dyes*

Bromophenol blue Xylene cyanol FF

Denaturing gels4.0 100-500 nt 50 nt 230 nt5.0 70-400 nt 35 nt 130 nt6.0 40-300 nt 26 nt 105 nt8.0 30-200 nt 19 nt 75 nt

10.0 20-100 nt 12 nt 55 nt15.0 10-50 nt 10 nt 40 nt20.0 5-30 nt 8 nt 28 nt30.0 1-10 nt 6 nt 20 nt

Non-denaturing gels3.5 100-1000 bp 100 bp 460 bp5.0 80-500 bp 65 bp 260 bp8.0 60-400 bp 45 bp 160 bp12.0 50-200 bp 20 bp 70 bp15.0 25-150 bp 15 bp 60 bp20.0 5-100 bp 12 bp 45 bp

* Positions of the tracking dyes can be estimated only approximately because the dye front migrates as fuzzy band.

Tracking dye migration in agarose gels.

0.510 bp

100 bp

1 kb

10 kb

100 kb

1 1.5 2 2.5 3 3.5 4 4.5 5 5.5Agarose, %

Bromphenol blue in TBE buffer

Bromphenol blue in TAE buffer

Xylene cyanol FF in TBE buffer

Xylene cyanol FF in TAE buffer

DNA Base Pairs

Cytosine ------------------------------------ Guanine

Thymine ------------------------------------- Adenine

* Translation termination codon.** Codes for fMet if in the initiator position.

Codons and Assigned Amino Acids

First (5’) Second Third (3’)U C A G

U

PhePheLeuLeu

SerSerSerSer

TyrTyrTer*Ter*

CysCysTer*Trp

UCAG

C

LeuLeuLeuLeu

ProProProPro

HisHisGlnGln

ArgArgArgArg

UCAG

A

IleIleIle

Met (fMet)

ThrThrThrThr

AsnAsnLysLys

SerSerArgArg

UCAG

G

ValValVal

Val**

AlaAlaAlaAla

AspAspGluGlu

GlyGlyGlyGly

UCAG

Summary of Useful Conversion

1 Becquerel (Bq) = 1 disintegration per second = 2.7x10-11 Curies (Ci)

1 Ci = 3.7x1010 Bq = 37 GBq = 2.22x1012 disintegrations per minute (dpm) 1 mCi = 37 MBq = 2.22x109 dpm 1 μCi = 37 kBq = 2.22x106 dpm1 GBq = 27 mCi1 MBq = 27 μCi1 kBq = 27 nCi

Physical Properties of Some Common RadioisotopesRadioisotope Half-life Specific activity, MBq/mmol

32P 14.3 days up to 107

33P 25.4 days up to 107

35S 87.4 days up to 107

131I 8.04 days up to 105

125I 60 days up to 107

14C 5730 years up to 103

3H 12.43 years up to 106

* Positions of the tracking dyes can be estimated only approximately because the dye front migrates as fuzzy band. To use the data above, the following guidelines are recommended:

• Agaroseshouldbetopquality.

• Onlyfreshlypreparedelectrophoresisbuffersshouldbeused.

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Concentrations of Acids and Bases

* With some acids and bases, stock solutions of different molarity/normality are in common use.

Substance Formula MW Moles/liter* Grams/liter%

by weightSpecific gravity

ml/liter to prepare 1 M solution

Acetic acid, glacial CH3COOH 60.05 17.4 1045 99.5 1.05 57.5

Acetic acid CH3COOH 60.05 6.27 376 36 1.045 159.5

Formic acid HCOOH 46.02 23.4 1080 90 1.20 42.7

Hydrochloric acid HCl 36.5 11.6 424 36 1.18 86.2

Nitric acid HNO3 63.02 15.99 1008 71 1.42 62.5

Perchloric acid HClO4 100.5 11.65 1172 70 1.67 85.8

Phosphoric acid H3PO4 97.9 14.8 1445 85 1.70 67.7

Sulfuric acid H2SO4 98.1 18.0 1766 96 1.84 55.6

Ammonium hydroxide NH4OH 35.0 14.8 251 28 0.898 67.6

Potassium hydroxide KOH 56.1 13.5 757 50 1.52 74.1

Sodium hydroxide NaOH 40.0 19.1 763 50 1.53 52.4

Conversion FormulaC = A / x 103

C – mM concentration of compoundsA – observed absorbance at max (nm) – molar absorption coefficient (M-1 x cm-1)

(see section “Nucleotides & Primers” for ordering information)

molar absorption coefficient (absorbance at max for 1M solution at pH 7.0) * determined at pH 7.0** determined at pH 10.0

CompoundMW,

Da (acid form)max*, nm

, M-1 x cm-1

ATP 507 259 15400

CTP 483 271 9000

GTP 523 253 13700

UTP 484 262 10000

dATP 491 259 15200

dCTP 467 271 9300

dGTP 507 253 13700

dTTP 482 267 9600

ddATP 475 261 15200

ddCTP 451 271 9300

ddGTP 491 253 13600

ddTTP 466 267 9600

dm6ATP 507 265 15400

dm4CTP 481 274 13600

dm5CTP 481 279 8770

Aminoallyl-dUTP 523240 290

11900 7800

Biotin-11-dUTP 862240 289

10700 7100

NAD 664 260 18000

NADH 665 338** 6200

NADP 743 260 18000

NADPH 745 260 18000

Physical Constants of the Nucleoside Triphos-phates and Related Compounds

SI Unit Prefixes

Prefix Symbol Multipleyotta Y 1024

zetta Z 1021

exa E 1018

peta P 1015

tera T 1012

giga G 109

mega M 106

kilo k 103

hecto h 102

deka da 101

deci d 10-1

centi c 10-2

milli m 10-3

micro μ 10-6

nano n 10-9

pico p 10-12

femto f 10-15

atto a 10-18

zepto z 10-21

yocto y 10-24

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Common Conversions of Nucleic Acids

Spectrophotometric ConversionsNA A260 μg/ml mM (in nucleotides)

dsDNA 1 50 0.15

ssDNA 1 33 0.1

ssRNA 1 40 0.12

dsDNA 6.7 335 1

ssDNA 10.0 330 1

ssRNA 8.3 332 1

The average MW of a deoxyribonucleotide base = 333 DaThe average MW of a ribonucleotide base = 340 DaThe average MW of a deoxyribonucleotide base pair = 650 Da

Molar Conversions

Phage/Plasmid DNA bpQuantity

μg pmol

DNA 1000 1 1.52

0.66 1

pUC18/19 DNA 2686 1 0.57

1.77 1

pBR322 DNA 4361 1 0.35

2.88 1

SV40 DNA 5243 1 0.29

3.46 1

X174 DNA 5386 1 0.28

3.54 1

M13mp18/19 DNA 7250 1 0.21

4.78 1

phage DNA 48502 1 0.03

32.01 11

DNA/Protein Conversions1 kb of DNA = 333 amino acid 37 kDa10 kDa protein 0.27 kb DNA30 kDa protein 0.81 kb DNA50 kDa protein 1.32 kb DNA100 kDa protein 2.70 kb DNA

Estimation of Ends (3’ or 5’) ConcentrationCircular DNA pmol ends = pmol DNA x number of cuts x 2Linear DNA pmol ends = pmol DNA x (number of cuts x 2 + 2)Phage/Plasmid DNA pmol ends (1 μg)1000 bp DNA 3.04

linear pUC18/19 DNA 1.14

linear pBR322 DNA 0.7

linear SV40 DNA 0.58

linear X174 DNA 0.56

linear M13mp18/19 DNA 0.42

phage DNA 0.06

References1. Sambrook, J., et al., Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Labora-

tory, Cold Spring Harbor, N.Y., 2001. 2. Wallace, R.B., et al., Hybridization of synthetic oligodeoxyribonucleotides to phiX174 DNA:

the effect of single base pair mismatch, Nucleic Acids Res., 6, 3543-3557, 1979. 3. Butkus, V., et al., Synthesis and physical characterization of DNA fragments containing

N4-methylcytosine and 5-methylcytosine, Nucleic Acids Res., 20, 8467-8478, 1987.4. Sambrook, J., et al., Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Labora-

tory, Cold Spring Harbor, N.Y., 10.2-10.4, 2001.

Common Conversions of Oligonucleotides

Common Conversions of OligonucleotidesMolecular WeightMW = 333 x NConcentration of Oligonucleotides C (μM or pmol/μl) = A260 / (0.01 x N)C (ng/ml) = (A260 x MW) / (0.01 x N) MW – molecular weightA260 – absorbance at 260 nmN – number of bases

For Duplex Oligonucleotide shorter than 25 bp, “The Wallace Rule” (2)

Tm (in °C) = 2(A+T) + 4(C+G), where(A+T) – the sum of the A and T residues in the oligonucleotide and (G+C) – the sum of G and C residues in the oligonucleotide.

Presence of m5C in oligonucleotide increases melting temperature of duplex. Presence of m4C or m6A decreases melting temperature (3).

For Duplex DNA, <100 bp long (4)

Tm (in °C) = 81.5°C+16.6(log10[Na+])+0.41(%[G+C])-675/n-1.0mn – the number of bases in the oligonucleotidem – the percentage of base-pair mismatches

Melting Temperature of Duplex DNA and Oligo-nucleotides

Temperature Dependence of the pH of 50 mM Tris-HCl Solutions

pH of the 50 mM Tris-HCl solutions4°C 8.1 8.2 8.3 8.4 8.5 8.6 8.7 8.8 8.9 9.0 9.1 9.2 9.3 9.4

25°C 7.5 7.6 7.7 7.8 7.9 8.0 8.1 8.2 8.3 8.4 8.5 8.6 8.7 8.8

37°C 7.2 7.3 7.4 7.5 7.6 7.7 7.8 7.9 8.0 8.1 8.2 8.3 8.4 8.5

Name ConstantAvogadro’s Number 6.02214 x 1023 mol-1

Planck’s Constant 6.62608 x 10-34 J s

Boltzmann Constant 1.38066 x 10-23 J K-1

Ideal Gas Constant0.0821 L atm/mol K8.314 J/mol K6.24 x 104 cm3 torr/mol K

Speed of Light 2.9979 x 108 m/s

Atomic Mass Unit 1.66054 x 10-27 kg

General Physical Constants

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Amino Acids

Nonpolar (hydrophobic)Amino acid Structure

Alanine

Proline

Valine

Leucine

Isoleucine

Methionine

Phenylalanine

Tryptophan

AcidicAmino acid Structure

Aspartate

Glutamate

Polar, unchargedAmino acid Structure

Glycine

Serine

Threonine

Cysteine

Asparagine

Glutamine

Tyrosine

BasicAmino acid Structure

Lysine

Histidine

Arginine

Amino acidAbbreviation

Mr, DapKa values

pI3-letter 1-letter

pK1 (-COOH–)

pK2

(-NH3+)

pKR

(R group)

Nonpolar (hydrophobic)

Alanine Ala A 89.09 2.34 9.69 6.01

Proline Pro P 115.13 1.99 10.96 6.48

Valine Val V 117.15 2.32 9.62 5.97

Leucine Leu L 131.17 2.36 9.60 5.98

Isoleucine Ile I 131.17 2.36 9.68 6.02

Methionine Met M 149.21 2.28 9.21 5.74

Phenylalanine Phe F 165.19 1.83 9.13 5.48

Tryptophan Trp W 204.23 2.38 9.39 5.89

Polar, uncharged

Glycine Gly G 75.07 2.34 9.60 5.97

Serine Ser S 105.09 2.21 9.15 5.68

Threonine Thr T 119.12 2.11 9.62 5.87

Cysteine Cys C 121.16 1.96 10.28 8.18 5.07

Asparagine Asn N 132.12 2.02 8.80 5.41

Glutamine Gln Q 146.15 2.17 9.13 5.65

Tyrosine Tyr Y 181.19 2.20 9.11 10.07 5.66

Basic

Lysine Lys K 146.19 2.18 8.95 10.53 9.74

Histidine His H 155.16 1.82 9.17 6.00 7.59

Arginine Arg R 174.20 2.17 9.04 12.48 10.76

Acidic

Aspartate Asp D 133.10 1.88 9.60 3.65 2.77

Glutamate Glu E 147.13 2.19 9.67 4.25 3.22

www.biomatik.com 1-800-836-808990

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Nd

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RE

NC

E d

ATA

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14. A

PPEN

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www.fermentas.com518

For patent and license information see p.557

Technical Data

Genome Sizes

References1. GOLD™ Genomes online Database, for more complete data and updates, see http://www.genomesonline.org/.2. Genomes Atlas Database, for more complete data and updates, see http://www.cbs.dtu.dk/services/GenomeAtlas/.

Virus Length, nt Approx. MW, DaBacteriophage X174 5,380 3.5 x 106

Bacteriophage Lambda 48,502 3.1 x 107

Human Immunodeficiency Virus 1 9,181 3.1 x 106

Rous Sarcoma Virus 9,392 3.2 x 106

SARS Coronavirus 29,751 1.0 x 107

Simian Virus 40 (SV 40) 5,224 3.4 x 106

Vaccinia Virus 191,737 1.2 x 108

Variolla (Small pox) Virus 185,578 1.2 x 108

ArchaeaLength of DNA, nt

Approx. MW, Da

Halobacterium sp. NRC-1 2.01 106 1.31 109

Methanosarcina acetivorans C2A 5.75 106 3.74 109

Methanococcus jannaschii DSM2661 1.66 106 1.08 109

Methanosarcina mazei Go1 4.10 106 2.66 109

Methanobacterium thermoautotrophicum delta H 1.75 106 1.14 109

Pyrococcus horikoshii OT3 1.74 106 1.13 109

Pyrococcus abysii GE5 1.76 106 1.14 109

Pyrococcus furiosus DSM3638 1.91 106 1.24 109

Sulfolobus acidocaldarius DSM639 2.23 106 1.45 109

Sulfolobus solfataricus P2 2.99 106 1.94 109

Thermoplasma acidophilum 1.56 106 1.01 109

BacteriaLength of DNA, nt

Approx. MW, Da

Agrobacterium tumefaciens C58-Cereon 4.91 106 3.19 109

Aquifex aeolicus VF5 1.55 106 1.00 109

Bacillus subtilis 168 4.20 106 2.73 109

Bacillus halodurans 4.20 106 2.73 109

Bordetella pertussis Tohama I NCTC-13251 4.07 106 2.65 109

Chlamydophila pneumoniae CWL029 1.23 106 8.00 108

Deinococcus radiodurans R1 3.28 106 2.13 109

Escherichia coli K12 4.64 106 3.02 109

Escherichia coli 0157:H7 EDL933 4.10 106 2.66 109

Haemophilus influenzae KW20 1.83 106 1.19 109

Helicobacter pylori 26695 1.66 106 1.08 109

Lactobacillus acidophilus NCFM 1.99 106 1.29 109

Lactococcus lactis IL1403 2.36 106 1.53 109

Mycobacterium leprae TN 3.27 106 2.12 109

Mycobacterium tuberculosis H37Rv 4.45 106 2.89 109

Mycoplasma genitalium G037 5.80 105 3.77 108

Mycoplasma pneumoniae M129 8.16 105 5.30 108

Neisseria meningitidis Z2491 2.18 106 1.42 109

Salmonella typhimurium LT2 SGSC1412 4.86 106 3.16 109

Staphylococcus aureus MW2 2.82 106 1.83 109

Streptomyces coelicolor A3(2) 8.67 106 5.64 109

Streptococcus pneumoniae R6 2.04 106 1.33 109

Streptococcus pyogenes SF370 (M1) 1.85 106 1.20 109

Pseudomonas aeruginosa 6.26 106 4.07 109

Pseudomonas fluorescens Pt-5 7.07 106 4.60 109

Thermus thermophilus HB8 2.12 106 1.38 109

Vibrio cholerae N16961 4.00 106 2.60 109

EukaryotesLength of DNA, nt

Approx. MW, Da

Anopheles gambiae PEST (malaria mosquito)

2.78 108 1.80 1011

Arabidopsis thaliana (flowering plant) 1.15 108 7.47 1010

Caenorhabditis briggsae (soil-dwelling nematode)

1.04 108 6.76 1010

Caenorhabditis elegans (round worm) 1.21 107 7.86 109

Ciona intestinalis (ascidian tadpole) 1.16 108 7.54 1010

Drosophila melanogaster (fruit fly) 1.37 108 8.90 1010

Gallus gallus (chicken) 1.05 109 6.82 1011

Guillardia theta (chromophyte algae) 5.51 105 3.58 108

Homo sapiens (human) 3.15 109 2.07 1012

Mus musculus (mouse) ~3.00 109 ~1.95 1012

Neurospora crassa OR74A (filamentous fungus)

4.30 107 2.80 1010

Saccharomyces cerevisiae S288C (budding yeast)

1.21 107 7.86 109

Schizosaccharomyces pombe (fission yeast)

1.40 107 9.10 109

Pan troglodytes (chimpanzee) 3.02 109 1.96 1012

Plasmodium falciparum 3D7 (human malaria parasite)

2.29 107 1.49 1010

Oryza sativa japonica (rice) 4.20 108 2.73 1011

Calculation the number of copies of a template amount of genome DNA (μg) Avogadro constant (mol-1)Genome copy number = length of DNA (bp) 106 650

This calculation is based on the assumption that the average weight of a base pair (bp) is 650 Daltons.Example: Human genome copy number in 1 μg of genome DNA.

1 (μg) 6.022 1023 (mol-1)Genome copy number = = 2.94 105

3.15 109 (bp) 106 650

1-800-836-8089 www.biomatik.com 91

AppENdiX - tECHNiCAl rEFErENCE dAtA

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Perio

dic

Tabl

e of

the

Elem

ents

IAVI

IIA1

HHy

drog

en1.

0079

IIAIII

AIV

AVA

VIA

VIIA

2

He Heliu

m4.

0026

3

LiLi

thiu

m6.

941

4

BeBe

rylliu

m9.

0122

5

B Boro

n10

.811

6

CCa

rbon

12.0

11

7

NNi

troge

n14

.007

8

OOx

ygen

15.9

99

9

FFl

uorin

e18

.998

10

Ne Neon

20.1

8011

Na Sodi

um22

.990

12

Mg

Mag

nesi

um24

.305

IIIB

IVB

VBVI

BVI

IBVI

IBVI

IBVI

IIIB

IIB

13

AlAl

umin

ium

26.9

82

14

Si Silic

on28

.086

15

PPh

osph

orus

30.9

74

16

S Sulfu

r32

.066

17

ClCh

lorin

e35

.453

18

Ar Argo

n39

.948

19

KPo

tass

ium

39.0

98

20

Ca Calc

ium

40.0

78

21

ScSc

andi

um44

.956

22

TiTi

tani

um47

.897

23

VVa

nadi

um50

.942

24

CrCr

omiu

m51

.996

25

Mn

Man

gane

se54

.938

26

Fe Iron

55.8

47

27

Co Coba

lt58

.933

28

Ni Nick

el58

.693

29

Cu Copp

er63

.546

30

Zn Zinc

65.3

9

31

Ga Galliu

m69

.723

32

GeGe

rman

ium

72.6

1

33

As Arse

nic

74.9

22

34

SeSe

leni

um78

.96

35

BrBr

omin

e79

.904

36

Kr Kryp

ton

83.8

037

RbRu

bidi

um85

.468

38

SrSt

ront

ium

87.6

2

39

YYt

trium

88.9

06

40

ZrZi

rcon

ium

91.2

24

41

Nb Niob

ium

92.9

06

42

Mo

Mol

ybde

num

95.9

4

43

TcTe

chne

tium

[98]

44

RuRu

then

ium

101.

07

45

RhRh

odiu

m10

2.91

46

PdPa

lladi

um10

5.42

47

Ag Silve

r10

7.87

48

CdCa

dmiu

m11

2.41

49

In Indi

um11

4.82

50

Sn Tin

118.

71

51

SbAn

timon

y12

1.75

52

TeTe

lluriu

m12

7.60

53

IIo

dine

126.

90

54

Xe Xeno

n13

1.29

55

Cs Cesi

um13

2.91

56

Ba Bariu

m13

7.33

57-7

1

*

72

HfHa

fniu

m17

8.49

73

TaTa

ntal

um18

0.95

74

WTu

ngst

en18

3.85

75

ReRh

eniu

m18

6.21

76

OsOs

miu

m19

0.23

77

IrIri

dium

192.

22

78

PtPl

atin

um19

5.08

79

Au Gold

196.

97

80

Hg Mer

cury

200.

59

81

TlTh

aliu

m20

4.38

82

Pb Lead

207.

2

83

BiBi

smut

h20

8.98

84

PoPo

loni

um[2

09]

85

AtAs

tatin

e[2

10]

86

Rn Rado

n[2

22]

87

FrFr

anci

um[2

23]

88

Ra Radi

um[2

26.0

3]

89-1

03

* *

104

RfRu

ther

ford

ium

[261

]

105

Db Dubn

ium

[262

]

106

SgSe

abor

gium

[263

]

107

Bh Bohr

ium

[264

]

108

Hs Hass

ium

[269

]

109

Mt

Mei

tner

ium

[268

]

110 Uu

nUn

unni

lium

[271

]

57

LaLa

ntha

num

138.

91

58

Ce Ceriu

m14

0.12

59

PrPr

aseo

dym

ium

140.

91

60

NdNe

odym

ium

144.

24

61

PmPr

omet

hium

[145

]

62

Sm Sam

ariu

m15

0.36

63

EuEu

ropi

um15

1.97

64

GdGa

dolin

ium

157.

25

65

Tb Terb

ium

158.

93

66

DyDy

spro

sium

162.

50

67

Ho Holm

ium

164.

93

68

Er Erbi

um16

7.26

69

Tm Thul

ium

168.

93

70

YbYt

terb

ium

173.

04

71

LuLu

tetiu

m17

4.97

89

Ac Actin

ium

[227

]

90

Th Thor

ium

232.

04

91

PaPr

otac

tiniu

m23

1.04

92

UUr

aniu

m23

8.03

93

NpNe

ptun

ium

237.

05

94

PuPl

uton

ium

[244

]

95

AmAm

eric

ium

[243

]

96

Cm Curiu

m[2

47]

97

BkBe

rkel

ium

[247

]

98

CfCa

lifor

nium

[251

]

99

EsEi

nste

iniu

m[2

52]

100 Fm Fe

rmiu

m[2

57]

101 M

dM

ende

leviu

m[2

58]

102

NoNo

beliu

m[2

59]

103

LrLa

wre

nciu

m[2

62]

* La

ntha

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ser

ies

** A

ctin

ide

serie

s

Alka

li m

etal

s

Alka

line

earth

met

als

Tran

sitio

n m

etal

s

Poor

met

als

Solid

Liqu

id

Gas

Synt

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Br H TcCNo

nmet

als

Nobl

e ga

ses

Lant

hani

de s

erie

s

Actin

ide

serie

s

+1 -1 +1

+1

+1

+1

+1

+1

+2+2

+2

+2+2

+2

+3

+3

+3

+4

+4

+4

+4

+5

+3

+4

+5

+3

+5

+2

+3

+4

+5

+2

+3

+6

+2

+3

+4

+5

+6

+2

+3

+4

+5

+6

+2

+3

+4

+5

+6

+7

+2

+4

+7

+2

+3

+4

+6

+7

+3

+4

+6

+8

+2

+3

+4

+6

+8

+2

+3

+6

+2

+3

+2

+3

+4

+5

+2

+3

+4

+6

+2

+3

+2

+4

+2

+4

+6

+1

+2

+3

+1

+2

+3

+1

+3

+2

+2

+1

+2

+3

+3

+3

+1

+3

+1

+3

+2

+4

+4

+2

+4

+2

+4

+2

+4

-3 +2

+3

+4

+5 -3 +3

+5 -3 +3

+5 -3 +3

+5

+3

+5

-1 -2 -2 +2

+4

+6 -2 +4

+6 -2 +4

+6

+2

+4

+6

-1 -1 +1

+3

+5

+7 -1 +1

+3

+5 -1 +1

+5

+7 -1 +1

+3

+5

+7

- - -

+2

+2

+4

+6

+8

+2

+3

+3

+3

+4

+4

+3

+4

+4

+5

+3

+3

+4

+5

+6

+3

+3

+4

+5

+6

+2

+3

+3

+4

+5

+6

+2

+3

+3

+4

+5

+6

+3

+3

+4

+3

+4

+2

+3

+4

+3

+2

+3

+4

+3

+2

+3

+3

+2

+3

+3

+2

+3

+2

+3

+2

+3

+3

+3

6

CCa

rbon

12.0

11

Oxid

atio

n de

gree

Sym

bol

Nam

eAt

omic

wei

ght

IVA

Grou

p cl

assi

ficat

ion

+2

+4

Atom

ic n

umbe

r

www.biomatik.com 1-800-836-808992

AP

PE

Nd

Ix -

TE

CH

NIC

AL

RE

fE

RE

NC

E d

ATA

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Page 97: Bio-Reagents Oligo Synthesis Gene Synthesis Peptide ... Spnosors/biomatik_catalog_2… · A2115 ACrylAmidE Ultra Pure 100G C 3H 5NO Purity Conductivity (40%, Water) Melting Point

Perio

dic

Tabl

e of

the

Elem

ents

IAVI

IIA1

HHy

drog

en1.

0079

IIAIII

AIV

AVA

VIA

VIIA

2

He Heliu

m4.

0026

3

LiLi

thiu

m6.

941

4

BeBe

rylliu

m9.

0122

5

B Boro

n10

.811

6

CCa

rbon

12.0

11

7

NNi

troge

n14

.007

8

OOx

ygen

15.9

99

9

FFl

uorin

e18

.998

10

Ne Neon

20.1

8011

Na Sodi

um22

.990

12

Mg

Mag

nesi

um24

.305

IIIB

IVB

VBVI

BVI

IBVI

IBVI

IBVI

IIIB

IIB

13

AlAl

umin

ium

26.9

82

14

Si Silic

on28

.086

15

PPh

osph

orus

30.9

74

16

S Sulfu

r32

.066

17

ClCh

lorin

e35

.453

18

Ar Argo

n39

.948

19

KPo

tass

ium

39.0

98

20

Ca Calc

ium

40.0

78

21

ScSc

andi

um44

.956

22

TiTi

tani

um47

.897

23

VVa

nadi

um50

.942

24

CrCr

omiu

m51

.996

25

Mn

Man

gane

se54

.938

26

Fe Iron

55.8

47

27

Co Coba

lt58

.933

28

Ni Nick

el58

.693

29

Cu Copp

er63

.546

30

Zn Zinc

65.3

9

31

Ga Galliu

m69

.723

32

GeGe

rman

ium

72.6

1

33

As Arse

nic

74.9

22

34

SeSe

leni

um78

.96

35

BrBr

omin

e79

.904

36

Kr Kryp

ton

83.8

037

RbRu

bidi

um85

.468

38

SrSt

ront

ium

87.6

2

39

YYt

trium

88.9

06

40

ZrZi

rcon

ium

91.2

24

41

Nb Niob

ium

92.9

06

42

Mo

Mol

ybde

num

95.9

4

43

TcTe

chne

tium

[98]

44

RuRu

then

ium

101.

07

45

RhRh

odiu

m10

2.91

46

PdPa

lladi

um10

5.42

47

Ag Silve

r10

7.87

48

CdCa

dmiu

m11

2.41

49

In Indi

um11

4.82

50

Sn Tin

118.

71

51

SbAn

timon

y12

1.75

52

TeTe

lluriu

m12

7.60

53

IIo

dine

126.

90

54

Xe Xeno

n13

1.29

55

Cs Cesi

um13

2.91

56

Ba Bariu

m13

7.33

57-7

1

*

72

HfHa

fniu

m17

8.49

73

TaTa

ntal

um18

0.95

74

WTu

ngst

en18

3.85

75

ReRh

eniu

m18

6.21

76

OsOs

miu

m19

0.23

77

IrIri

dium

192.

22

78

PtPl

atin

um19

5.08

79

Au Gold

196.

97

80

Hg Mer

cury

200.

59

81

TlTh

aliu

m20

4.38

82

Pb Lead

207.

2

83

BiBi

smut

h20

8.98

84

PoPo

loni

um[2

09]

85

AtAs

tatin

e[2

10]

86

Rn Rado

n[2

22]

87

FrFr

anci

um[2

23]

88

Ra Radi

um[2

26.0

3]

89-1

03

* *

104

RfRu

ther

ford

ium

[261

]

105

Db Dubn

ium

[262

]

106

SgSe

abor

gium

[263

]

107

Bh Bohr

ium

[264

]

108

Hs Hass

ium

[269

]

109

Mt

Mei

tner

ium

[268

]

110 Uu

nUn

unni

lium

[271

]

57

LaLa

ntha

num

138.

91

58

Ce Ceriu

m14

0.12

59

PrPr

aseo

dym

ium

140.

91

60

NdNe

odym

ium

144.

24

61

PmPr

omet

hium

[145

]

62

Sm Sam

ariu

m15

0.36

63

EuEu

ropi

um15

1.97

64

GdGa

dolin

ium

157.

25

65

Tb Terb

ium

158.

93

66

DyDy

spro

sium

162.

50

67

Ho Holm

ium

164.

93

68

Er Erbi

um16

7.26

69

Tm Thul

ium

168.

93

70

YbYt

terb

ium

173.

04

71

LuLu

tetiu

m17

4.97

89

Ac Actin

ium

[227

]

90

Th Thor

ium

232.

04

91

PaPr

otac

tiniu

m23

1.04

92

UUr

aniu

m23

8.03

93

NpNe

ptun

ium

237.

05

94

PuPl

uton

ium

[244

]

95

AmAm

eric

ium

[243

]

96

Cm Curiu

m[2

47]

97

BkBe

rkel

ium

[247

]

98

CfCa

lifor

nium

[251

]

99

EsEi

nste

iniu

m[2

52]

100 Fm Fe

rmiu

m[2

57]

101 M

dM

ende

leviu

m[2

58]

102

NoNo

beliu

m[2

59]

103

LrLa

wre

nciu

m[2

62]

* La

ntha

nide

ser

ies

** A

ctin

ide

serie

s

Alka

li m

etal

s

Alka

line

earth

met

als

Tran

sitio

n m

etal

s

Poor

met

als

Solid

Liqu

id

Gas

Synt

hetic

Br H TcCNo

nmet

als

Nobl

e ga

ses

Lant

hani

de s

erie

s

Actin

ide

serie

s

+1 -1 +1

+1

+1

+1

+1

+1

+2+2

+2

+2+2

+2

+3

+3

+3

+4

+4

+4

+4

+5

+3

+4

+5

+3

+5

+2

+3

+4

+5

+2

+3

+6

+2

+3

+4

+5

+6

+2

+3

+4

+5

+6

+2

+3

+4

+5

+6

+7

+2

+4

+7

+2

+3

+4

+6

+7

+3

+4

+6

+8

+2

+3

+4

+6

+8

+2

+3

+6

+2

+3

+2

+3

+4

+5

+2

+3

+4

+6

+2

+3

+2

+4

+2

+4

+6

+1

+2

+3

+1

+2

+3

+1

+3

+2

+2

+1

+2

+3

+3

+3

+1

+3

+1

+3

+2

+4

+4

+2

+4

+2

+4

+2

+4

-3 +2

+3

+4

+5 -3 +3

+5 -3 +3

+5 -3 +3

+5

+3

+5

-1 -2 -2 +2

+4

+6 -2 +4

+6 -2 +4

+6

+2

+4

+6

-1 -1 +1

+3

+5

+7 -1 +1

+3

+5 -1 +1

+5

+7 -1 +1

+3

+5

+7

- - -

+2

+2

+4

+6

+8

+2

+3

+3

+3

+4

+4

+3

+4

+4

+5

+3

+3

+4

+5

+6

+3

+3

+4

+5

+6

+2

+3

+3

+4

+5

+6

+2

+3

+3

+4

+5

+6

+3

+3

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+3

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+2

+3

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+3

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+3

+2

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+3

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+3

+2

+3

+2

+3

+2

+3

+3

+3

6

CCa

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12.0

11

Oxid

atio

n de

gree

Sym

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Nam

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Page 98: Bio-Reagents Oligo Synthesis Gene Synthesis Peptide ... Spnosors/biomatik_catalog_2… · A2115 ACrylAmidE Ultra Pure 100G C 3H 5NO Purity Conductivity (40%, Water) Melting Point

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