ABSTRACTS New Biotechnology Volume 25S September 2009
(0 or 600mg/seedling). Inoculation with a mixture of myceliumfrom S. bovinus, L. laccata and L. determinus and with a mixture ofspores of P. tinctorius and S. citrinum enhanced plant development,measured bfertiliser. Rebiotechnol
The use of bculture, parespecially ware consideproductionthe inuenbiofertilizetative strainAzotobacterand one isoAs.Pp1) whand stimulaand used folates were iblack cumiof growth.
(Biofertimedicinal proselinum sawith the apand one fuobtained shproduced bmedicinal proselinum sa
Genetic trment strcultures o
Abrus precawhich is c
source of sweet principle compound glycyrrhizin (a triterpenoidsaponin). It can be used as a very good substitute for Liquorice.Genetic transformation and elicitation has proved to be an effec-
y tostuded tred piry r431d Npineneecatoermimeds wecon
1 A. p
celltolonic acromsformtaintureredcemebtai/v),plicalicyle inntrotranecate pohizin
, A. N
al standaneby 1anceof Tass s
S308 www.y shoot height, and plant biomass, without the need ofsults indicate that selected ECM fungi can be benecialogical tools in nursery production of P. pinaster.
zers and their signicance to environmentalinable agriculture
d Said Ali , H. Soliman, A. Abdallah, T. Moharram, S.
sity, Cairo, Egypt
iofertilizers has become a great hope for Egyptian agri-ticularly in the eld of production of medicinal plants,hen the economic and environmental points of viewred, since they reduce the environmental pollution andcosts, in addition to improving the quality. Therefore,ce of using locally isolated nitrogen-xing bacteria asrs were studied. The isolates used were three represen-s of either Azotobacter and Azospirillum (two isolates ofout of 60 isolates, these isolates were Az.NP7-Az.PR1,late of Azospirillum out of 60 isolates, this isolate wasich were screened for their activity in nitrogen xingtion effect by used root exudates of black cumin plantsr further inoculation studies as biofertilizers. These iso-solated at random from rhizosphere and rhizoplane ofn, parsley and fenugreek cultivars at the different stagesThe effect of inoculation with N2-xing bacteria.lizer) on growth plant characteristics of testedlants (Nigella sativa, Trigonella foenum-graecum and Pet-tivum) plants were studied. Inoculationwas conjugatedplication of four doses of mineral N, i.e. 0, 50%, 75%ll dose (100%) from the recommended doses. Resultsowed that the application of biofertilizers (N2-xers)etter growth and reduced the N requirement in manylants such as Nigella sativa, Trigonella foenum and Pet-tivum compared with untreated plants.
ansformation and elicitation as yield enhance-ategy for glycyrrhizin production by cellf Abrus precatorius L.
ara , V.K. Dixit
of Pharmaceutical Sciences, Sagar, India
torius L. (family Fabaceae) known as Indian liquoriceommon bedding deciduous vine. It is a very good
tive waIn thismediatimprovand haMTCC532 antion. OAbrus gof A. prcally gSkoogcultureculturepH, 27formed(driedzopus sascorbgistic puntranwas obcell culcompaenhanwere o(7.5%vThe apand saincreasthe cogeneticof A. prindicatglycyrr
Bactericoccushistopltiedto enhgrowthand m
elsevier.com/locate/nbtenhance secondary metabolites in plant cell cultures.y, we describe the amalgamation of Ti and Ri plasmid-ansformation ofAbrus precatorius and elicitation for theroduction of glycyrrhizin in the established crown galloot culture of A. precatorius. Agrobacterium tumefaciensand MTCC 2250 and Agrobacterium rhizogenes MTCCCIM5140 were used for the Abrus genetic transforma-e assay and polymerase chain reaction conrmed thetic transformation. High yielding transformed cell lineriuswas developed from leaf explant taken from asepti-nated Abrus plantlets, cultured on solid Murashige andium (0.8%agar) without growth regulators. Suspensionre developed on optimized media composition andditions (photoperiod of 12/12h light/dark cycle, 5.8C temperature and 120 rpm). The Agrobacterium trans-recatorius cell culture which further elicited with bioticpowder and culture ltrate of Aspergillus niger and Rhi-ifer) and abiotic elicitors (yeast extract, salicylic acid,id) at optimum concentration. This resulted in syner-otion of glycyrrhizin accumulation compared with theed cell culture. Glycyrrhizin productivity of 32.5mg/L
ed in 20 days of cultivation. The Abrus transformedshowed 2.5-fold increase in glycyrrhizin content whenwith untransformed callus (0.0126%DW). Maximumnts of 3.02-fold and 2.83-fold in glycyrrhizin contentned with Aspergillus niger (10%v/v) and R. stoloniferrespectively, 3rd and 6th day after elicitor treatment.tion of yeast extract (10mg/L), ascorbic acid (10M)ic acid (10M) resulted in 1.88, 1.92 and 1.65-foldglycyrrhizin content respectively when compared withl culture. Present study reports very rst successfulsformation of A. precatorius and study on cell cultures
orius capable to produce glycyrrhizin. These results alsotential to develop a suitable methodology to produceat large scale.
cetic acid production by plant associated bac-ential to alter endogenous IAA content andf Triticum aestivum L.
asim Sabri, S. Hasnain
the Punjab, Lahore, Pakistan
rains of Bacillus, Pseudomonas, Escherichia, Micro-Staphylococcus genera isolated from rhizosphere,
and phyllosphere of different plant species were iden-6S rDNA gene sequencing. Strains were evaluatedendogenous indole-3-acetic acid (IAA) content and
riticum aestivum var. Inqalab-91. Gas chromatographypectrometric (GCMS) analysis revealed that bacte-