BioFlo® 610 Fermentor

65 & 125 Liter Sterilizable-in-Place Mobile Systemsfor R&D through Production

Modular Design Provides Flexibility • Easilyaddorremovesystemcomponentsatanytime,

pre-orpost-deliverytoaccommodatechangesinyourprocessrequirements.

• Numerousportsinthevesselheadplateandsidewallprovideflexibilitytopositionprobes,additionvalves,pressuretransducerandmore,whereverneeded.

• Multiplegasflowoptionsletyouoptimizecontrol,basedonyourprocessneeds.ChooseoneortwoThermalMassFlowControllers,inavarietyofflowranges,orchooseaRotameterformanualflowcontrol.

• Awidevarietyofoptionsareoffered,includingSCADAsoftware,sprayballsforvesselclean-in-place,redundantpH/DOprobes,resterilizablesampleandadditionvalves,andmore.

Advanced Controller Optimizes Results• Simultaneouslyregulateupto32processloopsthroughthe

sophisticatedRPCReactorProcessController.

• Create,save,rename,deleteandloadupto10batchrecipestostandardizeyourprocessandreduceoperatorvariability.

• TrenduptoeightprocessparameterssimultaneouslyononescreenandexportprocessvaluedataforanalysisinExcelviatheUSBport.

• Built-insecurityfeaturesprovidetwousergroupsuniqueuser-definedpasswordsandautolog-out.

• Processscale-upandscale-downaremadeeasierbecausethesameRPCcontrollerisusedacrossourlineofbench-andintermediate-scalefermentorsystems.

Introducing the BioFlo 610 – an exceptionally compact and versatile, industrial Mobile Pilot Plant Fermentor with choice of 65 and 125 L sterilizable-in-place vessels for R&D through small-scale production.

This modular system is offered with a comprehensive set of standard off-the-shelf options for rapid initial delivery, as well as easy customization at any time, should your process needs change. The entire system is built-on a mobile skid that fits through virtually any doorway, making it easy to move and share between labs in research, pilot plant and cGMP environments.

The BioFlo 610’s Intuitive Touchscreen Interface Makes Advanced Operations User Friendly

The BioFlo® 610 Fermentor: Compact. Comprehensive. And Amazingly Versatile.

➔ EnterandviewsterilizationparametersandvalvesequencesfromtheSterilizationScreen.Acrossthebottomarequicklinkstoscreensforsynopticview,calibration,cascades,trends,pumps,alarmsandsetup.

➔ TrendGraphsmakeitsimpletotrackandexportdataonuptoeightprocessvariablesoverasixdayspan.Customizetheviewbyselectingtheparameters,colorsandintervalbetweensamplemeasurements.

➔ Simultaneouslyviewupto10setpoints,currentvalues,cascadeloopsandmoreontheSummaryScreen.All32parameterscanbereadilyviewedsimplybyscrollingupordown.SynopticScreen(shownonthefrontcover),providespictorialalternativeforviewingandeditingprocessvalues.

➔ Cascadeoneormorevariables(inthiscaseAgitationandO2)toachievesophisticatedprocesscontrol,basedonthevalueofanyotheroneormorevariables.Cascadesruninparallel,notjustserialsequence,foroptimizedcontrol.

➔ ReducethetimeandeffortneededtoverifyvesselintegritythroughthePressureHoldTestScreen.Simplyenterpressurizationsetpoint,holdtime,andallowablepressuredroptoactivatetheautomatedvalvesequence.APass/Failnotificationisprovidedforsystemstatusandrecordkeeping.

Modular, Industry-Standard Components enableeasycustomizationaswellasreadysourcingofreplacementparts

Optional Exhaust Gas Condenser reducesevaporationofvesselcontents

Four Removable Vessel Bafflesenhancemixing

Multiple Probe Options forpH,DO,Redox,2ndpH,and2ndDOareoffered

Two Foam/Level Conductivity Probes are assignabletopumps,alarmorcontrolloops

Illuminated Sight Glass withmomentarylightprovideclearviewingofvesselcontents

Resterilizable Drain Valveenablessteriletransferofvesselcontents

Sanitary Fittingsallowutilitiestobeconnectedinminutes

Multiple PG 13.5 Headplate Ports and Sanitary Style NBS Connection Portsareprovidedthroughoutthevessel,providingflexibilitytopositionsensorsandredundantprobeswhereverneeded.Theseportsareflushwiththevessel’sinterior,virtuallyeliminatingdeadlegstominimizecontaminationriskandsimplifycleaning

Optional Resterilizable Sample Valveenablessamplingvesselcontentsatanytimeduringyourprocessrun

Bottom Drive with Double Mechanical Seal and Rushton Style Impellerarestandard.Low-shearpitchedbladeandmarinebladeimpellersoptional

Built-in Load Cellsprovideadirectmeasureofvesselcontents,enablingintegratedcontrolofpumpsforharvestingorautomaticadditionofmedia,pH,DO,orfoamcontrolagents

Customizable PI Values or Factory Defaults canbeselectedformostprocessparametersMultiple Analog Inputs and Outputs,easilyaccessedfromthesideofthecontrolpanel,integrateexternaldevicesforoptimizedprocesssensingandcontrol

Automatic Vessel Pressure Controllerallowscontrolviathetouchscreeninterface

Adjustable-Angle, User-Friendly 15” Touchscreen Interfacesimplifiescontrolandprovidesaclearviewofprocessparameters

Three Built-in, Assignable Peristaltic Pumpsareprovidedforaddition,harvest,foam,level,etc.Controlsandconnectionsarealsoprovidedforeasyadditionofyourexternalpumps

Safety FeaturesincludeasanitaryrupturediskinthevesselandanASMEsafetyreleasevalveonthedrainjacket

Centrally-Located Gauge Panelprovidesquickreferencetobasicprocessutilities

ASME, CE and cETLus Certified andofferedwithoptionalvalidationpackagesforvirtuallyanymanufacturingneed

The BioFlo® 610 Fermentor: Compact. Comprehensive. And Amazingly Versatile.

➔ Resterilizable Addition Valve Array facilitatesmakingsterileadditions.Eachvesselcanaccommodateuptofouradditionports.Oneadditionportshown

➔ Optional Glycol Heat Exchangerenablesrapidcool-down.Closed-loop,eco-friendlydesigneliminatesneedforsingle-passcoolingwateringrowthmode,andreducesoverallwaterconsumption

Integrated System Includes Control Station with Touchscreen Interface, 65 or 125 Liter Vessel, and Mobile Piping Skid

Swing-Away Headplatemakesiteasytoaccessthevesselinteriorforcleaning

➔

BioFlo® 610 Fermentor Specifications*

Vessel

Total Volume 65.0 Liters 125.0 LitersWorking Volume 16 - 50 Liters 31 - 100 Liters

Construction• Aspect Ratio: 3:1 • Code Ratings: ASME/CE• Material of Construction: 316L SS • Vessel Pressure: 50 PSIG, Full vacuum• Vessel Access: Headplate • Finish: 20 Ra mechanically polished interior [standard]

Agitation Drive: Bottom drive, double-mechanical sealSpeed 50 - 700 rpm 50 - 500 rpmImpellers (3) Rushton-Style impellers standard. Low-shear marine blade and pitched blade optionalBaffles (4) Removable, 316 L stainless-steel

Ports

Headplate• (3) PG 13.5 [Level 1 probe/spare, Level 2 probe/spare, septum/spare]• (4) 1.5” NBS connect sanitary style [pressure gauge, exhaust, and (2) spray balls/septums/spares]• (1) 2” Vessel light

Upper Side Wall • (7) 1.5” NBS connect sanitary style [pressure transducer/spare, gas overlay/spare, vessel rupture device, and (4) addition valves/spares]• (1) 3” NBS connect sanitary style [vessel sight glass]

Lower Side Wall • (7) 1.5” NBS connect sanitary style [RTD, sample/spare, spare, sparger, and (3) DO/pH/Redox or combinations thereof]Bottom (1) 1” NBS connect sanitary style [radial diaphragm drain valve]

ControllerControl Station Controls one vessel with 32 control loops. Stores 10 recipes and eight process variables for trend graphing. Includes an

industrial touchscreen monitor/user interface, three built-in pumps, and connections for all utilities and communication signalsTouchscreen Interface/Display 15” Industrial touchscreen interface/display

PumpsStandard, Options & Control

Standard: Three built-in, assignable, peristaltic pumps. Control modes: Off, Prime, Base, Acid, Foam, Level 2 Wet, Level 2 Dry, Volume Add, Volume HarvestOptional: External variable-speed pumps can be added with totalizer and functionality of standard pumps

Speed Pumps 1 – 3: 100 rpm Fixed-speed duty cycle

Piping Skid

Construction • Material of construction: 316L SS • Gaskets/O-Rings: Class (VI) EPDM and Silicon

Aeration Standard: 1 Thermal Mass Flow Controller (TMFC) with single-gas controlOptional: 1 TMFC with 2-gas control, 1 Rotameter with either single-gas or 2-gas control, 2 TMFCs (2-gas control)

Gas Inlet Sparger/overlay filter housing with 0.2 µ absolute disposal filter. Overlay valve optional

Exhaust Line Line designed for minimal backpressure. Includes heater and 1.2 µ nominal exhaust filter and housing Automatic backpressure control

Temperature Control Line

• All systems come with automatic pressure hold and sterilization program• Operating temperature control range 10°C above water supply temperature to 50°C• Line designed to achieve 1°C/minute temperature rises, in the 30° - 50°C range• Optional: Glycol/chiller heat exchanger designed to remove 100 watts/L

Load Cell Provided for measuring vessel volumeSensor Options • pH / DO probe kits • Redundant pH / DO probe kits • Redox probe kitDimensions (W x D x H) • 42” x 31.5” x 94” • 122 cm x 86 cm x 239 cm

Additional Options

• Spray balls • Foam/level kits • Turbidity sensor/transmitter • Addition valve connector kit• Transfer lines • Sterile sampling kit • Addition vessels • Marine and pitched-blade impellers• 1 or 7 port septum • Utility regulator kit • Scales for addition vessel • Vessel passivation• Validation packages • Bottle holder • Low pressure seal • Additional sight glass

Utility Requirements & Connections

Process Air 30 PSIG, 75 SLPM 30 PSIG, 150 SLPMOxygen 30 PSIG, 32 SLPM 30 PSIG, 64 SLPMInstrument Air 80-100 PSIG, 2 scfm (56.5 SLPM)Process Steam 35 PSIG, 10 lb/hr 35 PSIG, 20 lb/hrUtility Steam 35 PSIG, 50 lb/hr 35 PSIG, 100 lb/hrFacility Water 30 PSIG, 3 GPM 30 PSIG, 4 GPMWater Return Less than 15 PSIG back pressureClean Condensate Gravity drainBiowaste Gravity drainGlycol/Chiller 30 PSIG, 4 GPM 30 PSIG, 8 GPMElectric 208-230V AC, single phase, 50/60 Hz, 15 A

Input/Output Connections & Comm Ports (Built into the back panel of Master Control Station)

External Devices Seven analog inputs and seven analog outputs for your external devices such as analyzers, sensors, external pumps, etc.

2 USB Ports Import firmware/software upgrades and export trend data. Connect optional 8-port serial box for scales, etc.

Communications Port For optional BioCommand® SCADA software

Regulatory Compliance

New Brunswick Scientific is ISO 9001:2008 certified. * Specifications subject to change without notice.

B-402410XC1109-PDFa

Contact us for your local NBS sales office or distributor:www.nbsc.com • bioinfo@nbsc.com

POBox4005,Edison,NJ08818-4005,USA1.800.631.5417•+1.732.287.1200

ASME