Biovet Catalog of products

4375, av. Beaudry, Saint-Hyacinthe QC J2S 8W2 CANADA Phone 450 771-7291 • Fax 450 771-4158

www.biovet.ca

1-888-8BIOVET (824-6838)

[email protected]

http://www.biovet-inc.com/

mailto:[email protected]

About Biovet

Biovet operates a USDA and CFIA certified laboratory offering a complete array of innovative diagnostic services for veterinarians. More than a reference in Veterinary diagnostic, our laboratory is accredited by SCC and MSDEFCC for microbiological analyzes on foods and drinking water and Non-drinking water.

Additionally, Biovet is the only Canadian company to develop, manufacture and market high performance animal diagnostic kits. The head office is located in Saint-Hyacinthe, within the city of biotechnology, with a subsidiary in the United States.

For further information, visit our web site at www.biovet.ca or contact us at:

[email protected]

USA1-877-8BIOVET

Canada & International 1-888-8BIOVET

http://www.biovet.ca/


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DIAGNOSTIC KITS

AGRI-FOOD xMAP® Salmonella Serotyping Assay Kit by Luminex®

AVIAN Poultry Check MP® MS-MG

Poultry Check MP® MS-MG-AI

xMAP® Flock Monitor IBDV-NDV-IBV-REO by Luminex®

BOVINE Bovichek® BoHV-1 gB (IBR)

Bovichek® Lepto HP

Bovichek® Leukemia – 2 or 5 plates

Bovichek® M. bovis (Improved) – 1 or 2 plate

Bovichek® M. avium ssp paratuberculosis (Johne’s)

Bovichek® Neospora

Bovine ENTERICHEK®

Bovine ENTERICHEK® CRYPTO

BVDV Ag – 2 or 5 plates

FMDV NSP-3B cELISA (multi-species)

Pathasure Enteritis 3


SWINE

Swinecheck MP® APP 1-9-11, 2, 3-6-8-15, 4-7, 5, 10 and 12

Swinecheck MP® APP 1-9-11, 2, 3-6-8-15, 4-7, and 5

Swinecheck MP® PRRS (NA + EU), SIV, PCV2

(other Elisa kits)


Swinecheck® APP 1-9-11

Swinecheck® APP 2

Swinecheck® APP 3, 6, 8

Swinecheck® APP 4, 7

Swinecheck® APP 5a, 5b

Swinecheck® Mix-APP

All kits available internationally with the following permit requirements:

CFIA-licensed;

CFIA-licensed for export – requires a permit to release veterinary biologics from CFIA in Canada;

Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA;

USDA approved;

Requires a Research and Evaluation import permit from the USDA in the US. This product is not licensed by the USDA and any claims made have not been substantiated by the USDA.

NEW SOON AVAILABLE

CFIA: Canadian Food Inspection Agency;

MSDEFCC: ministry of sustainable Development, Environment and the Fight against Climate Change;

SCC: Standards Council of Canada;

USDA: United States Department of Agriculture.

Rev. 2015-12-15

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SWINE (other Elisa kits) — [Continued]

Swinecheck® APP 10

Swinecheck® APP 12

Swinecheck® Mix-APP 10-12

Swinecheck® H1N1

Swinecheck® H3N2

Swinecheck® HPS

Swinecheck® PCV2 indirect – 2 or 5 plates

Swinecheck® PED indirect – 2 or 5 plates

Swinecheck® PRRSV type 1 and 2

Swinecheck® TGEV – 2 or 5 plates

Swinecheck® TGEV-PRCV – 2 or 5 plates

OVUCHECK® (Progesterone measurement) Ovucheck® Milk

Ovucheck® Plasma

Ovucheck® Premate 10-5

Ovucheck® Premate porcine

Ovucheck® Rapid Well

Ovucheck® Reader

OTHERS


Dermatophytes

Bio-Tubes: Blood sample test kit

Sampling Kit for collection of oral fluids

Diagnostic kits

-

Diagnostic kits

2014-10-22

xMAP® Salmonella Serotyping Assay Kit by

PRODUCT CODE: TRD-502

DESCRIPTION

The Luminex® xMAP® Salmonella Serotyping Assay (SSA) is a multiplex, nucleic acid-based assay for research or epidemiological use in identifying the serotype of a Salmonella isolate. It is not for diagnosis of Salmonella spp. contamination or infection. This assay has been evaluated with Salmonella isolates only.

SUMMARY AND EXPLANATION

The xMAP Salmonella Serotyping Assay is a microsphere-based, molecular serotyping assay that detects genes involved in the expression of serotype-specific antigens. To determine a Salmonella serotype, the somatic O antigen and all phases of the motility H antigen must be identified. By detecting the genes involved in antigen expression rather than the antigens directly, as in traditional plate or tube agglutination, rough and more problematic isolates can be identified quickly and without the need for motility antigen-phase inversion. Molecular based serotyping removes the subjectivity of agglutination analysis and is not prone to common issues such as antisera lot-to-lot inconsistency. The assay serotypes approximately 85% of the top 100 serotypes most commonly encountered in testing laboratories, while providing partial serotype information for many other serotypes. These serotypes represent greater than 90% of the serotypes routinely encountered in public health laboratory settings.

CHARACTERISTICS

Molecular assay with results that are identical to traditional serotyping (Kauffman-White Scheme)

Ability to serotype rough and problematic isolates

No phase inversions required

Increased reliability and efficiency with decreased costs

Removes subjectivity

Eliminates antisera lot to lot variance

Time to result: ~ 4 hrs vs. days/weeks

Biovet inc. 4375, Beaudry Ave St-Hyacinthe QC J2S 8W2 Canada T 1-888-8BIOVET F 450 771-2996

[email protected]

www.biovet.ca

ASSAY PROCEDURE TIME

1. DNA extraction 30 minutes

2. Amplification of O, H and AT genes 3 hours

3. Hybridization with Luminex beads 45 minutes

4. Add reporter and incubate 10 minutes

5. Plate reading

6. Data analysis


Diagnostic kits

Diagnostic kits


[email protected]

www.biovet.ca

2014-12-23

Poultry Check MP® MS-MG

PRODUCT CODE: TRM-555

DESCRIPTION

Poultry Check MP® Ms-Mg Assay is a multiplexed fluorometric immunoassay (MFIA) intended for detecting antibodies to Mycoplasma synoviae (MS) and Mycoplasma gallisepticum (MG) in chicken serum.


Mycoplasma synoviae (MS) and Mycoplasma gallisepticum (MG) are two major bacterial pathogens for poultry flocks. The surveillance of poultry flocks for MS and MG relies on regular testing of serum samples for the presence of specific antibodies.

CHARACTERISTICS

Multiplexed fluorometric immunoassay (MFIA)

Results in less than 2 hours

460 tests per kit ASSAY PROCEDURE INCUBATION TIME/ TEMP.

1. Preparation of samples and reagents

2. Incubation of samples and controls 60 minutes / 19°C to 26°C

3. Serial washings

4. Incubation of conjugate 15 minutes / 19°C to 26°C

5. Serial washings

6. Revelation with substrate 15 minutes / 19°C to 26°C

7. Serial washings

8. Plate reading with a special reader

IMPORTANT :

Requires a permit to release veterinary biologics from CFIA in Canada. This product is not licensed by CFIA and any claims made have not been substantiated by CFIA.



Diagnostic kits


[email protected]

www.biovet.ca

2014-12-23

xMAP® Flock Monitor IBDV-NDV-IBV-REO by


DESCRIPTION

The Luminex® xMAP® Flock Monitor Assay is a mutiplex immunoassay intended for determining the presence of antibodies for Infectious Bursal Disease Virus (IBDV), Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV), and Avian Reovirus (REO) in chicken serum. This test kit is not indicated for diagnosis of disease in individidual birds.


Serological testing is routinely used to monitor antibody response to vaccination against Infectious Bursal Disease Virus (IBDV), Newcastle Disease Virus (NDV), Infectious Bronchitis Virus (IBV), and Avian Reovirus (REO). Luminex® xMAP® Flock Monitor allows determining antibody titers against these four viruses in a single assay.

CHARACTERISTICS


Results in less than 2 hours

460 tests per kit

ASSAY PROCEDURE INCUBATION TIME/ TEMP.

1. Preparation of samples and reagents

2. Incubation of samples and controls with the bead mix

60 minutes / 19°C to 26°C

3. Washings

4. Incubation of the detection antibody 30 minutes / 19°C to 26°C

5. Washings

6. Incubation of the reporter 30 minutes / 19°C to 26°C

7. Washings

8. Resuspending beads

9. Plate reading

10. Data analysis


IMPORTANT :

Approved by USDA.


Diagnostic kits

Diagnostic kits


[email protected]

www.biovet.ca

2014-12-23

BOVICHEK® BoHV-1 gB


DESCRIPTION

This kit is based on an immunoenzymatic assay for the detection of antibodies against bovine herpes virus 1 (BoHV-1) in bovine serum using BoHV-1-gB specific monoclonal antibodies.


Infectious bovine rhinotracheitis (IBR) is a highly contagious disease caused by BoHV-1. The virus which affects domestic and wild cattle is present worldwide but has been eradicated from several countries. The disease is characterized by clinical signs of the upper respiratory tract (rhinitis, conjunctivitis, tracheitis) accompanied by signs of general illness (fever, depression, inappetence, abortion). Moreover the virus can also infect the genital tract and cause pustular vulvovaginitis and balanoposthitis. Mortality is usually low and numerous infections are subclinical. Following infection the virus persists in a latent state for the entire life of the animals. It can be occasionally reactivated eventually resulting in virus shedding without clinical signs.

CHARACTERISTICS

2 x 96 tests

Sensitive, specific, and reproducible

Rapid (about 2 hrs 30)

Easy to perform


1. Dispense samples and controls and incubate 1 hour at 37°C

2. Wash

3. Dispense conjugate and incubate 1 hour at 37°C

4. Wash

5. Dispense substrate and incubate 15 minutes at room temperature

6. Dispense stop solution and read at 450 nm


IMPORTANT :



Diagnostic kits


[email protected]

www.biovet.ca

2014-12-23

BOVICHEK® Lepto HP


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Leptospira pomona and hardjo in bovine serum.


Controls and bovine serum samples are incubated in wells coated with either L. pomona or L. hardjo antigens (Ag). The antibodies (Ab) specific to L. pomona or L. hardjo present in positive serum samples will bind the Ag in the wells. After several washes to eliminate unbound substances, a conjugate (protein G coupled to peroxydase) is added. After incubation the excess of this conjugate is eliminated by a second series of washes and its attachment to bovine antibodies is revealed with a chromogenous substrate. The conjugate, if present, reacts with the substrate and a blue color develops. The reaction is then stopped and the optical densities are read. The intensity of the color is proportional to the quantity of Ab in the serum samples.

CHARACTERISTICS

Indirect ELISA

Specific antigens of Leptospira Hardjo and Pomona

Fast execution: less than 2 hours

96 tests for Leptospira Hardjo et Pomona kit


1. Serum dilution

2. Samples incubation and control 30 minutes at 23 + 2°C

3. Successive washes

4. Conjugate incubation 30 minutes at 23 + 2°C


6. Reading with substrate 15 minutes at 23 + 2°C

7. Stopping enzymatic reaction

8. Measuring optical densities

9. Calculation and interpretation of results


IMPORTANT :



Diagnostic kits


[email protected]

www.biovet.ca

2015-04-30

BOVICHEK® Leukemia – 2 or 5 plates

PRODUCT CODE: TRM-506/509

DESCRIPTION

This kit is an immunoenzymatic assay intended for the detection of Bovine Leukemia Virus (BLV) antibodies in cattle serum and milk.


BLV is a retrovirus which causes enzootic bovine leukosis (EBL). Most infections with BLV are subclinical, but a proportion of adult cattle develop persistent lymphocytosis, and eventually lymphosarcomas (tumours) in various organs. Clinical signs, if present, depend on the organs affected.

BLV is present in blood lymphocytes and in tumour cells as provirus integrated into the DNA of the cell. It is also found in the cellular fraction of various body fluids (e.g. saliva, milk). Natural transmission depends on the transfer of infected cells (e.g. during parturition). Some blood-sucking insects may also transmit the virus mechanically. Artificial transmission occurs, especially by blood-contaminated needles, surgical equipment, gloves used for rectal examinations, etc.

Infection with BLV in cattle gives rise to a persistent antibody response. Antibodies can first be detected 3-16 weeks after infection. They are present in both serum and milk. Maternally derived antibodies may take up to 6 or 7 months to disappear. The antibodies most readily detected are those directed towards the envelope glycoprotein gp51 of the virus.

Routine diagnosis of BLV infection is based on the detection of specific antibodies. Serological testing is used in certification programs, eradication programs and for commercial exchanges.

CHARACTERISTICS

Blocking ELISA: antigens specific to BLV (gp 51 protein)

Rapid (less than 2 hours)

Tests per kit: o 2 plates: up to 184 samples tested individually or pooled o 5 plates: up to 460 samples tested individually or pooled

(up to 10 serum samples or 2 milk samples per pool)



1. Preparation of samples

2. Incubation of samples and controls 1h00 / 37ºC


4. Incubation with conjugate 30 min / 37ºC


6. Revelation with substrate 10 min / room temperature

7. Stop the reaction

8. Reading optical densities 450 nm

IMPORTANT :



Diagnostic kits


[email protected]

www.biovet.ca

2014-12-23

BOVICHEK® M. avium ssp paratuberculosis (Johne’s)


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Mycobacterium avium ssp paratuberculosis (Map) in bovine serum.


Map is the etiological agent of Johne’s disease (synonym: paratuberculosis). This disease affects several ruminant species. Map multiplies in the intestinal mucosa and induces a progressive enteritis resulting in chronic diarrhea, wasting, drop in milk production and eventually death. The diagnosis of Johne’s is based on the demonstration of Map in the feces or tissues or of Map specific antibodies in the serum or milk. The most sensitive serological assays to detect Map specific antibodies are enzyme linked immune enzymatic assays (ELISA). The sensitivity of these tests increases with the progression of the infection. Some animals free from Map have antibodies directed against other environmental mycobacteria that may cause non-specific reactions. Pre-treatment of their sera with selected mycobacteria allows removing these non-specific antibodies.

CHARACTERISTICS

Test per kit: up to 92 samples

Indirect ELISA

Absorption of the samples with selected mycobacteria

Results within two hours



1. Incubation of the samples with the absorbent

30 minutes at 23°C ± 2°C

2. Incubation of the absorbed samples 30 minutes at 23°C ± 2°C

3. Successive washings

4. Incubation with the conjugate 30 minutes at 23°C ± 2°C

5. Successive washings


7. Incubation with the substrate 30 minutes at 23°C ± 2°C

8. Plate reading (450 nm)

IMPORTANT :



Diagnostic kits


[email protected]

www.biovet.ca

2015-02-26

BOVICHEK® M. bovis – 1 or 2 plates


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Mycoplasma bovis (M.bovis) in bovine serum and milk.


Epidemiologic studies have revealed the pathogenic properties of M. bovis in cattle. M. bovis is one of the pathogens responsible for mastitis in the cow and pneumopathy in the calf. M. bovis is also involved in abortion. Serodiagnosis is a valuable tool to diagnose M. bovis infections since immune response is early, important, stable, and persistent in time.

CHARACTERISTICS

Indirect ELISA: Specific antigens to M. Bovis

Interpretation based on positivity level


Tests per kit: o 1 plate: 48 samples tested as duplicate o 2 plates: 96 samples tested as duplicate



2. Incubation of samples and controls 30 min / 23°C


4. Incubation with conjugate 30 min / 23°C


6. Revelation with substrate 10 min / 23°C




IMPORTANT :



Diagnostic kits


[email protected]

www.biovet.ca

2015-12-15

BOVICHEK® Neospora


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Neospora caninum in bovine serum and milk.


Neospora caninum is a protozoa identified by Dubey et al. in 1988. In cattle, this protozoa is responsible for an important proportion of spontaneous abortions. Once the animal is infected, the chance of it undergoing abortion is 2- to 3-fold greater than in noninfected animals. Furthermore, if abortion does not occur, the animal will likely, 8 times out of 10, give birth to infected cattle.

CHARACTERISTICS

Indirect ELISA: Specific antigens to N. caninum (Strain NC-1)


Maximum number of samples tested per kit : 2 x 90

When considering suspicious results as positive: - the relative sensitivity of this test is 99.4% (95% CI = 96.1 – 100%) - the relative specificity is 99.1% (95% CI = 98.1 – 99.6%).









8. Reading optical densities 405 nm and reference filter at 490 nm


IMPORTANT :



Diagnostic kits


[email protected]

www.biovet.ca

2014-10-20


APPLICATION

BOVINE ENTERICHEK is a lateral flow immunochromatography assay allowing to detect Escherichia coli K99, Rotavirus, Coronavirus or Cryptosporidium in bovine feces present in their environment. Each kit contains enough reagents to examine 5 fecal samples for each of the 4 above mentioned agents. The test is particularly user-friendly and can be performed on the farm itself.

DISEASE DESCRIPTION

Neonatal diarrhea is a major disease of calves. The most important infectious agents causing the disease are Rotavirus, Coronavirus, Escherichia coli K99 and Cryptosporidium. Identifying these agents in fecal samples allows to determine to which agents calves are exposed and to take appropriate control measures.

CHARACTERISTICS

Lateral flow immunochromatography assay

Qualitative assay

Reagents ready to use

Rapid (15 minutes)

Tests per kit : 5 tests for 4 agents


1. Dilute fecal samples

2. Immerse strips into diluted fecal samples 10 min, room temperature

3. Visual reading


Trousses diagnostiques


[email protected]

www.biovet.ca

2014-11-21


APPLICATION

La trousse ENTERICHECK CRYPTO est un test d’immunochromatographie latérale qui permet de détecter la présence de Cryptosporidium dans les matières fécales qui sont présentes dans leur environnement. Chaque trousse contient les réactifs suffisants pour examiner 10 échantillons de matières fécales. Grâce à la facilité d’emploi de la trousse, les tests peuvent être réalisés sur la ferme même.

DESCRIPTION DE LA MALADIE

Cryptosporidium est un des principaux agents de diarrhée néonatale chez le veau. L’identification de cet agent dans les matières fécales permet de déterminer ceux auxquels les veaux sont exposés afin de prendre les mesures de contrôle appropriées.

CARACTÉRISTIQUES

Test d’immunochromatographie latérale

Test qualitatif

Composantes prêtes à l’emploi

Exécution rapide : 15 minutes

Nombre de tests par trousse : 10


1. Diluer les matières fécales

2. Immerger les bandelettes dans les matières fécales diluées.

10 min, température de la pièce

3. Lecture visuelle


Diagnostic kits


[email protected]

www.biovet.ca

2015-04-30

BVDV Ag – 2 or 5 plates


DESCRIPTION

This kit is an immunoenzymatic assay intended for the detection of Bovine Viral Diarrhea virus (BVDV) in tissues and blood of persistently infected cattle.


Bovine Viral Diarrhea virus (BVDV) is responsible for a variety of economically important disorders in cattle (diarrhea, abortion, pneumonia, etc.). Foetal infections with BVDV during the first trimester of pregnancy can result in the birth of persistently infected (PI) calves. Such animals excrete large quantities of the virus throughout their entire life. Removal of PI animals is the corner stone of efficient BVDV control programs.

CHARACTERISTICS

Capture ELISA: specific detection of BVDV antigens

Rapid: less than 3.5 hours

Tests per kit:

o 2 plates: 192 tests

o 5 plates: 460 tests

PROCÉDURE GÉNÉRALE TEMPS D’INCUBATION/ TEMP.

1. Preparation of samples 15-30 minutes

2. Incubation of samples and controls 1 hour/room temperature


4. Incubation with first conjugate 1 hour/room temperature


6. Incubation with second conjugate 1 hour/room temperature


8. Revelation with substrate 5 minutes/room temperature

9. Reading the results


IMPORTANT :

CFIA-licensed.


Diagnostic kits


[email protected]

www.biovet.ca

2014-12-23



DESCRIPTION

This kit is an immunoenzymatic assay intended for the detection of Rotavirus, Coronavirus and Escherichia coli (E. coli) K99 antigens in bovine feces.


Diarrhea problems in the calf are often caused by Rotavirus, Coronavirus, or E. coli K99. These problems can lead to important economical losses since affected animals generally show reduced weight gain, sometimes resulting in death. Quick identification of the pathogen involved helps in disease control. It is recommended to test the feces of at least 3 calves per infected herd throughout symptom evolution.

CHARACTERISTICS

Indirect ELISA: Specific Antibodies to Rotavirus, Coronavirus and E. coli K99

Rapid (Less than 2 hours)

Qualitative test

Test per kit : 1 well is needed to test each agent Example: 30 fecal samples can be tested for the 3 agents 94 fecal samples can be tested for 1 agent only





4. Incubation with conjugates 30 min / 23°C


6. Revelation with substrates 15 min / 23°C

7. Reading the results Visual


IMPORTANT :

CFIA-licensed for export – requires a permit to release veterinary biologics from CFIA in Canada.


Diagnostic kits


[email protected]

www.biovet.ca

2014-12-23



DESCRIPTION

This kit is an immunoenzymatic assay intended for the detection of Rotavirus, Coronavirus, Escherichia coli (E.coli) K99 and Crytosporidium parvum antigens in bovine feces.


Diarrhea problems in the calf are often caused by Rotavirus, Coronavirus, E. coli K99 or Cryptosporidium parvum. These problems can lead to important economical losses since affected animals generally show reduced weight gain, sometimes resulting in death. Quick identification of the pathogen involved helps in disease control. It is recommended to test the feces of at least 3 calves per infected herd throughout symptom evolution.

CHARACTERISTICS

Indirect ELISA: Specific Antibodies to Rotavirus, Coronavirus, E. coli K99, Cryptosporidium parvum


Qualitative test

Test per kit : 1 well is needed to test each agent Example: 22 fecal samples can be tested for the 4 agents 94 fecal samples can be tested for 1 agent only





4. Incubation with conjugates 30 min / 23°C


6. Revelation with substrates 15 min / 23°C

7. Reading the results Visual


IMPORTANT :



Diagnostic kits

SW IN E

Diagnostic kits


[email protected]

www.biovet.ca

2015-04-30

SWINECHECK MP® APP 1-9-11, 2, 3-6-8-15, 4-7, and 5


DESCRIPTION

Swinecheck MP® APP assay is a mutiplex immunoassay intended for determining the presence of antibodies to Actinobacillus pleuropneumoniae (APP) serogroups 1-9-11, 2, 3-6-8-15, 4-7, and 5 in swine serum.


Swinecheck MP® APP assay is a microsphere-based multiplexed fluorometric immunoassay (MFIA)-type antibody detection test. A total of 5 distinct microsphere sets (coated with either APP 1-9-11, APP 2, APP 3-6-8-15, APP 4-7, or APP 5 antigens) are incubated in diluted swine serum and then washed. Successive incubations with biotinylated anti-swine IgG (Detection Antibody) and streptavidin R-phycoerythrin (SA-PE) reporter follow, each succeeded by a wash step to remove unbound materials. Users place plates in the plate analyzer, which captures the fluorescence intensity of the microspheres and SA-PE reporter. An S/P ratio of the SA-PE on the antigen-coated microspheres above the provided cut-offs indicates antibody is present in the sample.

CHARACTERISTICS


5 plates

Results available within 2.5 hours

Tests per kit: up to 445 samples



2. Incubation of samples and controls 1hr / 19-26°C

3. Serial washings

4. Incubation with conjugate A 30 min / 19-26°C

5. Serial washings

6. Incubation with conjugate B 30 min / 19-26°C

7. Serial washings

8. Plate reading


IMPORTANT :



Diagnostic kits


[email protected]

www.biovet.ca

2015-12-17

SWINECHECK MP® PRRSV SIV and PCV2


DESCRIPTION

Swinecheck MP® PRRSV, SIV, PCV2 assay is a mutiplex immunoassay intended for determining the presence of antibodies to Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) type 1 and 2, Swine Influenza Virus (SIV) type A, and Porcine Circovirus type 2 (PCV2) in swine serum.


Swinecheck MP® PRRSV, SIV, PCV2 assay is a microsphere-based multiplex fluorescent immunoassay (MFIA)-type antibody detection test. A total of 4 distinct microsphere sets [coated with either PRRSV NA (type 2), PRRSV EU (type 1), SIV, and PCV2 antigens] are incubated in diluted swine serum and then washed. Successive incubations with biotinylated anti-swine IgG (Detection Antibody) and streptavidin R-phycoerythrin (SA-PE) reporter follow, each succeeded by a wash step to remove unbound reagent. Users place plates in the plate analyzer, which captures the fluorescence intensity of the microspheres and SA-PE reporter. An S/P ratio of the SA-PE on the antigen-coated microspheres above the provided cut-offs indicates antibody is present in the sample.

CHARACTERISTICS


5 plates

Results available within 2.5 hours

Tests per kit: up to 445 samples



2. Incubation of samples and controls 1hr / 19-26°C

3. Serial washings

4. Incubation with secundary antibodies 30 min / 19-26°C

5. Serial washings

6. Incubation with fluorescing reporter 30 min / 19-26°C

7. Serial washings

8. Plate reading


IMPORTANT :



Diagnostic kits


[email protected]

www.biovet.ca

2015-04-30

SWINECHECK® APP 1-9-11


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 1, 9, 11 in porcine serum.


Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotypes 1, 9, 11 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.

CHARACTERISTICS

Indirect ELISA: Specific antigens to serotypes 1, 9, 11


Tests per kit: 184 samples





4. Incubation of conjugate 30 min / 23°C




8. Reading the optical densities 405 nm (reference filter at 490 nm)

IMPORTANT :




Diagnostic kits


[email protected]

www.biovet.ca

2015-04-30

SWINECHECK® APP 2


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotype 2 in porcine serum.


Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotype 2 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.

CHARACTERISTICS

Indirect ELISA: Specific antigens to serotype 2












IMPORTANT :




Diagnostic kits


[email protected]

www.biovet.ca

2015-04-30

SWINECHECK® APP 3, 6, 8


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 3, 6, 8 in porcine serum.


Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotypes 3, 6, 8 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.

CHARACTERISTICS

Indirect ELISA: Specific antigens to serotypes 3, 6, 8












IMPORTANT :




Diagnostic kits


[email protected]

www.biovet.ca

2015-04-30

SWINECHECK® APP 4, 7


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 4, 7 in porcine serum.


Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotypes 4, 7 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.

CHARACTERISTICS

Indirect ELISA: Specific antigens to serotypes 4, 7












IMPORTANT :




Diagnostic kits


[email protected]

www.biovet.ca

2015-04-30

Swinecheck® APP 5a, 5b


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 5a, 5b in porcine serum.


Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotypes 5a, 5b is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.

CHARACTERISTICS

Indirect ELISA: Specific antigens to serotypes 5a, 5b











8. Reading optical densities 405 nm (reference filter at 490 nm)


IMPORTANT :



Diagnostic kits


[email protected]

www.biovet.ca

2015-04-30

SWINECHECK® Mix-APP


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 1-2-9-11, 3-6-8-15 and 4-5-7 in porcine serum.


Pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is an important swine disease that can result in considerable economic losses. Serological testing is a major diagnostic tool to detect herds infected with APP or to certify the absence of infection. There are presently 15 recognized serotypes of APP. As the economical importance of these serotypes varies significantly depending on the country, serological testing is usually limited to the most relevant serotypes and is based on serotype specific tests.

In herds considered to be free of several serotypes, a screening test able to simultaneously detect antibodies against the most relevant serotypes would simplify testing and reduce testing costs. In case of positive or suspect reactions, samples can be examined using relevant serotype specific tests.

CHARACTERISTICS

Indirect ELISA: Specific antigens to Actinobacillus pleuropneumoniae


Test per kit: 180 samples tested for serotypes 1-2-9-11 and 4-5-7, 184 samples tested for serotypes 3-6-8-15











IMPORTANT :



Diagnostic kits


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2015-04-30

SWINECHECK® APP 10


DESCRIPTION




CHARACTERISTICS













IMPORTANT :




Diagnostic kits


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2015-04-30

SWINECHECK® APP 12


DESCRIPTION




CHARACTERISTICS













IMPORTANT :




Diagnostic kits


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2015-04-30

SWINECHECK® Mix-APP 10-12


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Actinobacillus pleuropneumoniae serotypes 10-12 in porcine serum. It can be used in complement to kit Swinecheck® mix-APP 1-2-9-11, 3-6-8-15, and 4-5-7.


Pleuropneumonia caused by Actinobacillus pleuropneumoniae serotypes 10 and 12 is an important porcine disease that can result in considerable economic losses. Herds are often chronically infected without clinical symptoms. However, following a stressful situation, several clinical cases with mortality can occur. Since horizontal transmission through airways is quite important and infection is usually inapparent, serology becomes an indispensable tool for its detection.

CHARACTERISTICS

Indirect ELISA: Specific antigens against APP serotypes 10 and 12


Test per kit: 184 samples











IMPORTANT :



Diagnostic kits


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2015-06-15

SWINECHECK® H1N1 – 2 or 5 plates


DESCRIPTION

This is an immunoenzymatic assay for the detection of swine influenza (H1N1) antibodies in porcine serum.


The influenza virus is responsible for swine flu, one of the most common diseases throughout the world. Swine flu is contagious and mainly characterized by coughing, anorexia, lethargy, and nasal discharge. Other characteristics, although seldom observed, include abortion for sows and death for piglets. This virus has several subtypes, the most common being H1N1 and H3N2. The differentiation between the subtypes is important since it will optimize vaccine selection.

CHARACTERISTICS

Blocking ELISA: Specific antigens to swine influenza (H1N1)

Results available within 3 hours

Tests per kit:

o 2 plates: 180 samples

o 5 plates: 450 samples



2. Incubation of samples and controls 1 hour / 23°C


4. Incubation with monodonal antibody 15 min / 23°C


6. Incubation with conjugate 1 hour / 23°C






IMPORTANT :



Diagnostic kits


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2014-04-30

SWINECHECK® H3N2


DESCRIPTION

This is an immunoenzymatic assay for the detection of swine influenza (H3N2) antibodies in porcine serum.


The influenza virus is responsible for swine flu, one of the most common diseases throughout the world. Swine flu is contagious and mainly characterized by coughing, anorexia, lethargy, and nasal discharge. Other characteristics, although seldom observed, include abortion for sows and death for piglets. This virus has several subtypes, the most common being H1N1 and H3N2. The differentiation between the subtypes is important since it will optimize vaccine selection.

CHARACTERISTICS

Indirect ELISA: Specific antigens to swine influenza (H3N2)












IMPORTANT :




Diagnostic kits


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2015-04-30

SWINECHECK® HPS


DESCRIPTION

This is an immunoenzymatic assay for the detection of antibodies against Haemophilus parasuis (HPS) in porcine serum.


HPS is responsible for Glasser syndrome, a condition characterized by septicaemia which results in arthritis and meningitis. To date, 15 different serotypes have been identified with serotypes 1, 5, 10, 12, 13 and 14 being the most pathogenic.

CHARACTERISTICS

Indirect ELISA: Specific antigens to Haemophilus parasuis.


Test per kit: 92 samples tested as simplicate









8. Reading of optical densities 405 nm and reference filter at 490 nm

IMPORTANT :




Diagnostic kits


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2014-04-30

SWINECHECK® PCV2 indirect – 2 or 5 plates


DESCRIPTION

Swinecheck® PCV2 IgG indirect is an immunoenzymatic assay for the detection of IgG antibodies to porcine circovirus type 2 (PCV2) in porcine serum.


PCV2 has been associated to various clinical signs in weaned and growing pigs (the so-called Porcine Circovirus Associated Diseases, PCVAD), the most important being the Post-Weaning Multisystemic Wasting Syndrome (PMWS). Other frequent conditions associated with PCV2 include Porcine Dermatitis and Nephropathy Syndrome (PDNS), enteritis, myocarditis, and abortions or stillbirths.

Diagnosis of PCVAD relies on the demonstration of typical lesions associated with the presence of PCV2 nucleic acids or antigens. Measurement of antibodies (serology) may be helpful in evaluating virus exposure. It is also useful to evaluate the decrease of maternally derived antibodies and to implement efficient vaccination programs. Numerous serological assays have been developed to detect PCV2 antibodies (IFA, IPMA, ELISA, etc.). Enzyme-Linked Immuno Assays (ELISA) are the most popular as they are easy to perform and to be automatized.

CHARACTERISTICS


Recombinant antigen (PCV2 capsid)

Indirect quantitative ELISA

Results within two hours


1. Incubation of the samples 60 minutes at 23°C ± 2°C

2. Serial washings

3. Incubation with the conjugate 30 minutes at 23°C ± 2°C

4. Serial washings

5. Incubation with the substrate 10 minutes at 23°C ± 2°C


7. Interpretation of the results


IMPORTANT :



Diagnostic kits


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2015-04-30

SWINECHECK® PED indirect – 2 or 5 plates


DESCRIPTION

This kit is based on an immunoenzymatic assay (ELISA) for the detection of antibodies to Porcine Epidemic Diarrhea Virus (PEDV) in porcine serum samples.


Porcine epidemic diarrhea (PED) is a swine disease characterized by acute diarrhea and vomiting. The disease is very contagious and affects pigs of all ages. The mortality may reach 100% in young piglets but is negligible in mature pigs.

PED is caused by a coronavirus distinct from the other porcine coronaviruses more especially the transmissible gastroenteritis virus (TGEV). PED was first reported in Europe in the late ’60 and is now prevalent in Asian South East and more recently in the USA.

PED diagnosis relies on the demonstration of PEDV nucleic acids or antigens in various specimens or of PEDV antibodies in serum. Several serological tests have been developed to detect PEDV antibodies. Enzyme-Linked Immuno Assays (ELISA) are easy to perform and to be automatized.

CHARACTERISTICS

Indirect ELISA: Specific antigens to PEDV

2 breakable plates/kit

Results available within 4 hours

Tests per kit:

o 2 plates: up to 184 samples

o 5 plates: up to 460 samples



2. Incubation of samples and controls 1hr / 23°C


4. Incubation with conjugate A 1 hr / 23°C


6. Incubation with conjugate B 1 hr / 23°C






IMPORTANT :



Diagnostic kits


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2015-04-30

SWINECHECK® PRRSV type 1 and 2 indirect


DESCRIPTION

Swinecheck® PRRSV type 1 and 2 indirect is an immunoenzymatic assay for the detection of antibodies to porcine reproductive and respiratory syndrome virus (PRRSV) type 1 and 2 strains in porcine serum or plasma samples (EDTA or heparin).


Porcine reproductive and respiratory syndrome virus (PRRS) is caused by the PRRSV which comprises two major genotypes: type 1 (“European”, EU) and type 2 (“North-American”, NA). The disease appeared in the late ’80 in Europe and North America and is now present in most swine producing countries. PRRS is characterized by reproductive problems such as increased abortion in late gestation, mummification and stillbirth as well as increased mortality rates and pneumonia in young pigs.

CHARACTERISTICS


Indirect ELISA

Detects antibodies to both type 1 and 2 of PRRSV

Results within less than two hours


1. Incubation of the samples 30 minutes at 23°C + 2°C

2. Serial washings

3. Incubation with the conjugate 30 minutes at 23°C + 2°C

4. Serial washings

5. Incubation with the substrate 15 minutes at 23°C + 2°C




IMPORTANT :



Diagnostic kits


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2015-04-30

SWINECHECK® TGEV recombinant – 2 or 5 plates


DESCRIPTION

This kit is based on an immunoenzymatic assay for the detection of antibodies against transmissible gastroenteritis virus (TGEV) in porcine serum.


Transmissible gastroenteritis (TGE) is a highly contagious enteric disease of swine caused by TGEV. TGEV affects swine of all ages. It multiplies in the digestive and respiratory tracts and causes vomiting and diarrhea. The mortality rate in piglets under 2 weeks of age is near 100%. In older pigs, the disease is milder and can even occasionally go unnoticed.

A fast and accurate diagnosis of TGE is essential to prevent dissemination of the disease. Diagnosis is mostly based on viral identification. However detection of antibodies is very useful in older pigs that do not always display typical signs of disease. Serological tests are also regularly required for commercial exchanges.

Antibodies against TGEV can be detected using the virus neutralization assay (VN). However, the VN test requires cell culture facilities and the use of infectious TGEV which limits its use to specialized laboratories.

Moreover, it is very time consuming and results are available after several days only. By contrast, enzyme-linked immunosorbent assays (ELISA) can be run in most laboratories and results are available within a few hours.

Use of monoclonal antibodies specific to TGEV in a blocking ELISA assay allows the detection of TGEV antibodies whereas antibodies directed against porcine respiratory coronavirus (PRCV), a closely related swine coronavirus, are not detected. The specificity of the assay is improved by using a recombinant protein as antigen.

CHARACTERISTICS

Blocking ELISA: Specific antigens to TGEV


Test per kit:

o 2 plates: 184

o 5 plates: 460




2. Incubation of samples and controls 1h00 / 37°C







IMPORTANT :

CFIA-licensed.


Diagnostic kits


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2015-04-30

SWINECHECK® TGEV/PRCV recombinant – 2 or 5 plates


DESCRIPTION

This kit is based on an immunoenzymatic assay for the detection and differentiation of antibodies against transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) in porcine serum.


Transmissible gastroenteritis (TGE) is a highly contagious enteric disease of swine caused by TGEV. TGEV affects swine of all ages. It multiplies in the digestive and respiratory tracts and causes vomiting and diarrhea. The mortality rate in piglets under 2 weeks of age is near 100%. In older pigs, the disease is milder and can even occasionally go unnoticed.

PRCV is a respiratory variant of TGEV which has lost the ability to colonize the digestive tract. PRCV infects swine of all ages and can cause benign respiratory infections. Its close relationship with TGEV complicates the diagnosis of TGE.

A fast and accurate diagnosis of TGE is essential to prevent dissemination of the disease. Diagnosis is mostly based on viral identification. However detection of antibodies is very useful in older pigs that do not always display typical signs of disease. Serological tests are also regularly required for commercial exchanges.

As antibodies against TGEV and PRCV show complete cross-reaction by neutralization, the differentiation of antibodies aimed against these two viruses is impossible by seroneutralization testing. However, the use of monoclonal antibodies in a competitive ELISA assay allows the detection and differentiation of infections caused by these two viruses. The specificity of the assay is improved by using a recombinant protein as antigen.

CHARACTERISTICS

Blocking ELISA: Specific antigens to TGEV


Test per kit:

o 2 plates: 88 samples tested for each virus

o 5 plates: 220 samples tested for each virus




2. Incubation of samples and controls 1h00 / 37°C







IMPORTANT :

CFIA-licensed.


Diagnostic kits

O V U C H E C K

( P r o g e s t e r o n e m e a s u r e m e n t )

Diagnostic kits


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2015-04-30

OVUCHECK® Milk


DESCRIPTION

The OVUCHECK® MILK ELISA is an immunoenzymatic test which provides a simple, reliable and precise measurement of progesterone in whole milk from dairy cows. The concentration range covered by the reagents is 1 to 30 ng/mL. Each kit contains sufficient reagents for up to 92 tests plus four standards. OVUCHECK® MILK is used for oestrus detection and assessment of pregnancy status/luteal function in cows.


The OVUCHECK® MILK test is based on the competitive binding of unlabelled progesterone present in the standard or whole milk sample, and a fixed quantity of progesterone labeled with the enzyme alkaline phosphatase (AP), to binding sites on a limited amount of specific progesterone antibodies. The wells are pre-coated with antibodies, providing a solid phase for the convenient separation of the bound progesterone from the free progesterone in the milk. After incubation, all components other than those bound to the plate wells are washed away. The amount of bound AP-labelled progesterone remaining on the wells is inversely proportional to the concentration of the unlabelled progesterone present in the sample. The bound labelled progesterone is then measured by reacting the AP with its substrate during a second incubation. The colour produced is measured spectrophotometrically and the concentration of progesterone in the milk is determined from a standard curve. Alternatively, the colour can be interpreted visually.

CHARACTERISTICS

Qualitative test

Competitive binding of progesterone

Standard curve determined by progesterone standards (1, 5, 10 and 30 ng/ml)

Tests per kit: 92 possible tests



1. Preparation of substrate

2. Incubation of samples, standards and conjugate 30 min / AT


4. Incubation with substrate 30 min / AT


6. Reading of the results 405 nm

AT: Ambiant Temperature

Diagnostic kits


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2015-04-30

OVUCHECK® Plasma


DESCRIPTION

The OVUCHECK® PLASMA ELISA kit is an immunoenzymatic test which provides a simple, reliable and precise measurement of progesterone in plasma or serum of cows and bitches. Each kit con-tains sufficient reagents for up to 92 tests plus four standards. OVUCHECK® PLASMA is used for oestrus detection and assessment of pregnancy status and luteal function.


The OVUCHECK® PLASMA test is based on the competitive binding of unlabelled progesterone present in the standard or sample, and a fixed quantity of progesterone labelled with the enzyme alkaline phosphatase (AP) (conjugate), to binding sites on a limited amount of specific progester-one antibodies. The wells are pre-coated with antibodies, providing a solid phase for the capture of the progesterone present in the samples, the standards or the conjugate. After incubation, all components other than those bound to the plate wells are washed away. The amount of bound AP-labelled progesterone remaining on the wells is inversely proportional to the concentration of the unlabelled progesterone present in the sample. The bound labelled progesterone is then measured by making the AP react with its substrate during a second incubation. The colour pro-duced is measured spectrophotometrically and the concentration of progesterone in the sample is determined from a standard curve.

CHARACTERISTICS

Qualitative test


Standard curve determined by progesterone standards (1, 2.5, 5 and 10 ng/mL)









6. Reading of the results 405 nm


Diagnostic kits


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2015-04-30

OVUCHECK® Premate 5 and 10

PRODUCT CODE: TRM-540-541

DESCRIPTION

OVUCHECK® PREMATE is a semi-quantitative test for the measurement of progesterone in a drop of plasma or serum of the bitch. The quantity of progesterone present is indicated by a change in colour, which is compared with high and low progesterone standards. This kit is quick and simple to use. It gives reliable information which allows you to:

- plan mating at the optimum time - determine the time of whelping - investigate the causes of infertility (anovular cycles, silent heats with normal

ovulation)


Hormonal changes occurring in the bitch in the course of pro-oestrus, oestrus, beginning of dioestrus, and gestation are described below.

At the end of pro-oestrus, the drop in oestrogen levels, secreted by the maturing ovarian follicle, causes the luteinising hormone (LH) surge. Ovulation normally happens 24 to 48 hours after the LH surge.

The follicle gradually produces progesterone above basal levels (<0.5 ng/mL) a few days before the LH surge. When the LH surge occurs, progesterone levels may reach 2 to 4 ng/mL.

At the time of ovulation, the progesterone level is generally between 4 and 10 ng/mL. This normally happens 11 to 13 days after the start of pro-oestrus (characterized by the presence of blood and swelling of the vulva).

After ovulation, progesterone levels continue to rise to reach maximal levels 2 to 3 weeks after the start of dioestrus. However, there are large variations between breeds and individuals. The determination of progesterone level with OVUCHECK® PREMATE in association with other elements such as the behaviour of the dog and vaginal cytology allows determination of the time of ovulation and therefore the optimal time for mating.

At the end of gestation, 12 to 24 hours before giving birth, the level of progesterone falls again to reach values of less than 2 ng/mL. Thus, a level of progesterone higher than the low standard of the OVUCHECK® PREMATE test shows that parturition will not occur in the next 12 to 24 hours.


CHARACTERISTICS

Semi-quantitative test


Rapid (less than 1 hour)

Tests per kit: 5 tests Premate 5

10 tests Premate 10








Diagnostic kits


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2015-04-30

OVUCHECK® Premate porcine


DESCRIPTION

OVUCHECK® PREMATE PORCINE is an immunoenzymatic (ELISA) kit which provides a simple and reliable measurement of progesterone in plasma or serum of female swine (gilts, sows). OVUCHECK® PREMATE PORCINE is intended for assessment of ovarian function and provides a reliable aid to sow reproduction management. Each kit contains sufficient reagents for up to 28 tests.


The OVUCHECK® PREMATE PORCINE test is based on the competitive binding of unlabelled progesterone present in the standard or sample, and a fixed quantity of progesterone labelled with the enzyme alkaline phosphatase (AP) (conjugate), to binding sites on a limited amount of specific progesterone antibodies.

The wells are pre-coated with specific progesterone antibodies, providing a solid phase for the capture of the progesterone present in the samples, the standards or the conjugate. After incubation, all components other than those bound to the plate wells are washed away. The amount of bound AP-labelled progesterone remaining on the wells is inversely proportional to the concentration of the unlabelled progesterone present in the sample. The bound labelled progesterone is then measured by making the AP react with its substrate during a second incubation. The colour obtained with the sample is compared to that produced by two standards of 2.5 and 5.0 ng of progesterone/mL.

CHARACTERISTICS

Competitve ELISA

Semi-quantitative test

2 progesterone standards (2.5 et 5.0 ng/mL)

Tests per kit: 28


Diagnostic kits


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2015-04-30

OVUCHECK® Rapid Well


DESCRIPTION

OVUCHECK® RAPID WELL is used to measure the level of progesterone in a drop of cow’s milk. The quantity of progesterone present is indicated by a change in colour, which is compared with a standard. OVUCHECK® RAPID WELL is a simple and rapid test which provides a reliable aid to cow fertility management.


The amount of progesterone is almost zero at oestrus (heat) and then increases and remains at a high level. The progesterone level falls suddenly 17 to 20 days after the last heat, indicating the onset of the next cycle. This fall does not occur if the animal is pregnant.

CHARACTERISTICS

Qualitative test

Rapid (Less than 30 minutes)




1. Incubation of samples, standard and conjugate 5 min / AT


3. Incubation of substrate B and C 5 min / AT




Diagnostic kits


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2015-04-30

OVUCHECK® Reader


DESCRIPTION

OVUCHECK® Reader is a Convertible Well-strip/Cuvette Reader and is a portable miniphotometer designed to do ELISA testings in either 1x8/1x12 microwell strips or round/square cuvettes by interference filter for wavelength of 400-700nm.

OVUCHECK® Reader is a user-friendly ELISA reader. All operations are carried out with only two touch buttons by following instruction on the LCD display, and results can be obtained within two seconds.

OVUCHECK® Reader undertakes testings with an extreme economic Reagent consumption, in microwell at 0.3ml and in cuvette at 0.5ml.


OVUCHECK READER® can be use with Biovet Ovucheck® kits to measure progesterone levels in milk or blood.

CHARACTERISTICS

portable, compact, accurate, easy to use and affordable

Holders for 12mm diameter tubes or 9 x 13mm well strips.

Houses multiple filters, including the 405 nm and 550 nm filters

Changing filters and holders is fast and very easy.

Instructions that are as simple as using a video device.

Measurement accuracy comparable to more expensive devices.


Diagnostic kits

OTHE R S

Diagnostic kits


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2015-04-30

Dermatophytes


DESCRIPTION

This is a culture medium for rapid isolation and identification of dermatophytes.


In order to directly apply this method on samples taken in surgery or in the field, this kit is easy to use, giving the possibility of having a diagnosis in a few days. It contains specific nutrients, which facilitate the growth of dermatophytes and selective antibiotics which prevent the growth of non-pathogenic saprophytic mycetes and bacteria. The interpretation relies on the pH color indicator, which immediately shows the presence of dermatophytes by a color change, because Microsporum, Trichophyton and Epidermophyton produce alkaline metabolites.

CHARACTERISTICS

Rapid isolation and identification of dermatophytes.

Color change: 24-72 hours in presence of dermatophytes

Tests per kit: 10 tests / package


1. Carry out the sampling (hair, skin, scab, etc)

2. Place the sample on the edge of the culture medium.

3. Incubate at room temperature 48 hours / 23°C

4. Leave the sample in position for about 10 to 14 days to identify the colonies.


Diagnostic kits


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2015-04-30

Bio-Tubes® - Blood sample test kit


MATERIALS PROVIDED

100 Bio-Tubes® with screw caps.

MATERIALS REQUIRED BUT NOT PROVIDED

100 hypodermic needles 18G 1 ½ (single use)

Rubber bands

Rubbing alcohol

Gauze

Permanent marker

Stickers

PROCEDURE

1. Apply a rubber band at the base of the ear like a tourniquet

2. Rub the outside of the ear with alcohol

3. Allow to air dry

4. Attach the needle to the hub of the Bio-Tube

5. Gently insert the needle into the largest vein of the ear

6. Let the blood flow into the tube by gravity (can be harvested in a 1.5 ml)

7. Carefully remove the needle from the vein

8. Remove the rubber band at the base of the ear

9. Apply pressure on the puncture site to start clotting

10. Remove the needle from the hub of the Bio-Tube

11. Dispose of the needle appropriately

12. Unscrew the Bio-Tube and remove the hub with the pipe

13. Close the Bio-Tube with a screw cap

14. Identify the Bio-Tube with a permanent marker or a sticker

15. Let the Bio-Tube clot at room temperature for 30-45 minutes

16. Place the Bio-Tube in a Ziploc® bag

17. Bio-Tubes can be refrigerated before shipping to the laboratory

18. Complete a request form

19. Place the Ziploc® bag inside another Ziploc® bag with the request

20. Send to the laboratory in a cooler with ice packs


Diagnostic kits


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2015-04-30

Sampling Kit for collection of oral fluids


MATERIALS PROVIDED

cotton rope (about 30 feet)

10 tubes 50 ml

10 pairs of vinyl gloves

10 small plastic bags

10 medium plastic bags

MATERIALS REQUIRED BUT NOT PROVIDED

1 pair of scissors

Permanent marker

PROCEDURE

1. Fold the cotton rope in half and fix tightly in an easily accessible place for the animals

2. The free ends of the rope should be at shoulder height of the animals in no way contacting the ground.

3. Leave the rope in place for about thirty minutes

4. When the rope is sufficiently soaked, untie it

5. Place the tip that was chewed by animals in the plastic bag

6. Twist it with your hands to express the oral fluids into the plastic bag

7. Remove the rope

8. Cut one corner of the plastic bag

9. Pour the oral fluids into the Falcon tube

o The laboratory requires a minimum 3 ml of fluid

10. Close the Falcon tube tightly

11. Identify the tube with a permanent marker or a sticker

12. Place it in a plastic bag

13. Immediately put the bag in a cooler on ice packs

14. If samples cannot reach the laboratory the same day, freeze them (-20°)

15. Complete a request form

16. Place the plastic bag inside another bag with the request

17. Send that to the laboratory in a cooler with ice packs




2. Addition of samples, standards and conjugate and incubation

15 min/RT

3. Washings

4. Addition of substrate and incubation 15 min/RT

5. Stopping of the enzymatic reaction

6. Revelation with substrate

7. Stopping of the enzymatic reaction

8. Comparison of sample and standard colorations

RT: Room Temperature

See reverse for Sampling kits and diagnostic Services

Sampling Kits Bio-Tubes® - Blood sampling kit (PRRS) Collection of oral fluids (Influenza, PCV2, PRRS, PEDV)

Biovet is a biotechnology company providing unique expertise in the field of diagnostics for

animal health and agro-industry specialists. Biovet operates a USDA and CFIA certified* laboratory offering a complete array of innovative diagnostic services for veterinarians. Biovet employs more than 50 people including 15 scientists.

Two laboratories in North America to meet your diagnostic needs.

Canada Biovet Canada 4375, Beaudry avenue Saint-Hyacinthe, QC J2S 8W2 Canada Tel.: 450-771-7291 Fax: 450-771-4158 Toll free : 888-824-6838 [email protected]

USA Biovet USA 9025 Penn Avenue South Bloomington, MN 55431 USA Tel.: 952-884-3113 Fax: 952-884-3473 Toll free : 877-824-6838 [email protected]

888 824-6838 [email protected]

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