Training Course of Microbial Resources Information Management and Utilization for Developing Countries

BSL-3 Laboratory

Facility and Management

Dr. Yiping ZhuSep. 14 2016

Pathogens and BSL-3 laboratory

There are about 10 million microbe species have

been identified in the world.

Among those, only about 400 microbe species are

pathogenic to humans, and a little more than 100

species are high pathogenic, but they can ause very

serious infecious diseases.

Nowadays, merging and reemerging infecious

diseases (SARS, Avian flu, Ebola, Zaka…) are big

threat to human socity.

We need to learn how to deal with, and how to control

the diseases.

A BSL-3 or BSL-4 laboratory is required when we

handling high pathogenic microorganisms.

What does a BSL-3 Lab look like?

When you go into a BSL-3 Laboratory…

5

2

3

1

4

5

2

3

4

1 Biosafety Cabinet

Personal Protection Equipment

Biohazard Waste Container

Biohazard Warning Signs

Access control

Directed Air Flow

6

6

The Sandwich structure of BSL-3 Lab

BSL-3 Lab Design Requirements:

a. 2 door entry (interlock)

b. Solid easily cleanable surfaces

(floor, walls, ceiling)

c. Coved floors

d. HEPA (high efficiency

particulate air filter) filtered air

supply and exhaust

e. Negative pressure and

directed air flow

f. Sealed penetrations

g. Hands-free sink near exit door

h. Eyewasher and Shower

i. Pass through autoclave

j. Sewage reatment system

Why build a BSL-3 Laboratory?

BSL-3 Laboratories are designated for handling high pathogenic

microbes that may cause serious or potentially lethal disease

through the inhalation route of exposure.

P3 and 3 Ps:

Physical Containment level 3 Lab

Protect people

Protect samples

Protect environment

What build up a BSL-3 Laboratory?

BiosafetyManagement

BSL-3 Lab

Facility & Equipments

EnsureBiosafety

Before start to build a BSL-3 Lab

Seismic safety assessment

2

When design and build a BSL-3 Lab

GB19489-2008:

Laboratories-General requirements

for biosafety

GB50346-2011:

Architectural and technical code

for biosafety laboratories

Commissioning and pass the Third Party Inspection

Capability accreditation by CNAS

China National Accreditation Service for Conformity Assessment

Laboratory qualification approval by

the Ministry of Health

The approval of handling high pathogenic microorganisms by

Beijing Municipal Health Bureau

Before start runing a BSL-3 Lab

Access control

When runing a BSL-3 Lab

Camera

Local police

Biosecurity

Protects hazards

from dangerous

people

Biosafety

Management

Biosafety

Protects people

from dangerous

hazards

Key points of Biosafety Management

Waste

1

2

5

6

3

4

Biosafety

Management system

Facility

Routine work

Operation

Staff

Material

Risk awearness

Safe operition skill

Emergency response Quiz

Operition test

Ability assessment

Regular health check

Immunization

Serum storage

Counseling

Medical services and first aid

1. Staff management

Training

Healthsurveillance

Evaluation

Heinrich's Law: that in a workplace, for every accident that causes a

major injury, there are 29 accidents that cause minor injuries and 300

accidents that cause no injuries.

Why staff training is primary efficient?

Classifications Level of Hazard

Risk Group 1 No or low individual and community risk.

Risk Group 2 Moderate individual risk, low community risk.

Risk Group 3 High individual risk, low community risk.

Risk Group 4 High individual and community risk.

2. Biohazard materials management

Pathogens Handling of biohazard materials

NameRisk

groupCulture

Animal

trials

clinical

sample

Inactived

samples

Non-

infectious

samples

Mouse leukemia

virus1 BSL-1 ABSL-1 BSL-1 BSL-1 BSL-1

Hepatitis B virus 2 BSL-2 ABSL-2 BSL-2 BSL-1 BSL-1

High pathogenic

avian influenza

virus

3 BSL-3 ABSL-3 BSL-2 BSL-1 BSL-1

Ebola virus 4 BSL-4 ABSL-4 BSL-3 BSL-2 BSL-1

Handling of biohazard materials

.

SOPs

Good

practice

.

3. Operation management

Risk

assessment

and control

Emergency

response

Supplies

Clean

Decontamination

emergency response

Incident report

4. Routine work management

5. Facility equipment management

a. Daily inspection

b. Regular maintainence

c. Annual check and test

d. Annual maintinence

a. contaminated sharps

b. solid wastes

c. cultures, stocks

d. liquid wastes

e. animal carcasses

6. Waste management

a. autoclave

b. chemical

c. incineration

d. monitoring (autoclave tapes,

biological indicators)

.

.

.

.

Waste decontamination

Waste segregation

Support, Service, Serious

Ensure the Biosafety of Your Research

Yiping Zhu13466626489, zhuyp@im.ac.cn