1 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)

Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA).

CERISE Experiment“C.elegans RNAi space experiment”

JEM CERISE 31407

Instructor: Toru Mori Takashi Yamasaki

(mori.tooru@jaxa.jp)(yamasaki.takashi@jaxa.jp)

2 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)

Objectives

Perform the CERISE Experiment Operations according to ODFs without instructor’s assistance.

CERISE Experiment Explain the science objectives of the CERISE experiment.

List onboard outcomes from performing the CERISE experiment.

After this lesson the trainee will be able to:

Operation Flow

Outline the CERISE operations flow from the view point of the sample movement.

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Lesson Outline

CERISE Experiment . . . . . . . . . . . . . . . . . . 4

Operation Flow of CERISE . . . . . . . . . . . . . . . . 6

Key Points for CERISE Operations . . . . . . . . . . . 8

Appendix

1. Specification of Meas Exp Unit A . . . . . . . . . . . . . . . . . . . 11

2. Schematic of Meas Exp Unit A . . . . . . . . . . . . . . . . . . . 12

3. Photo of Meas Exp Unit A . . . . . . . . . . . . . . . . . . . 13

4. Acronyms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4 Training Purposes OnlyJEM CERISE 31407JMU-081217 (Ver. 1.1)

CERISE Experiment

CERISE Experiment (PI: Atsushi Higashitani)

Research Objectives1) Investigate whether RNAi occurs in space as the same manner as on the earth.2) Explore effects of microgravity on entire gene and protein expressions, and phosphoryl

ations beyond generations.

Onboard Outcomes from the Experiment

Cultivated nematodeDosimeterTemp LoggerMicrogravity environment data (taken by MMA)

ReturnedContained in Culture Bag Holder

Downlinked

Microgravity Measurement Apparatus (MMA)

Culture Bag Holder

Returned

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Intentionally Blank

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MELFI

Operation Flow of CERISE

Saibo Rack/CBEF Activation

Meas Exp Unit A (3) Retrieve

Saibo Rack/CBEF Deactivation

CBEF

Cultivation(4 days)

-80 deg C

Sample Preparation

Meas Exp Unit A (4) Instl into CBEF

MLT and MMA Deactivation

MMAM

icro

-G e

nviro

nmen

t m

easu

rem

ent

Sample Retrieve

MLT and MMA Activation

If they are off

As required

ULF3(TBD)

FLIGHT ULF3

CB Setup

CB Stow

CB

CB Activation

Microscope Observation

CB Deactivation

Microscope Observation

:Crew operations

:Crew/Ground cooperative operations

LEGEND:

:Ground operations

Meas Exp Unit A (2) Retrieve

Sample RetrieveMeas Exp Unit A (1) re-installation with dummy mass

Cultivation(4 days) Stow

Stow

Return

Meas Exp Unit A

Culture Bag Holder

ULF3

Launch

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Operation Flow of CERISE (cont’d)

Incubator Unit 1G

Incubator Unit Micro-G

Dummy Mass

nematode

nematode

Culture Bag Holder (4)

Pins

Remove

MELFI(-80 deg C)

Sample Preparation

Meas Exp Unit A (4)

Install

Cultivation (4 days)

Incubator Unit 1G

Incubator Unit Micro-G

CBEF

nematode nematode

nematode nematode

Sample Retrieve

Culture Bag x 15Dosimeter x 1Temp Logger x 1

Temp Logger

Rtrv

Rtrv

Rtrv

Stow

Stow

Stow

Culture Bag

Microscope Observation

Retrieve one Culture BagFrom each

Attach

Observe

Stow

Meas Exp Unit A Re-Instl with dummy mass

Culture Bag Holder(no nematode contained)

Install

CBEF

Re-Instl

Cultivation (4 days)

Sample Retrieve Microscope Observation

Clear Plate

CB Microscope

Install

Reuse

Dummy Mass

Reuse

Stow

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Key Points for CERISE Operations (CBEF)

NOTE: Underlined key points have been covered by CBEF Advanced Training.

Category Equipment Key Points

Crew Safety

Electrical Shock Sample Tray,

Centrifuge

Before attaching/detaching the Canisters to/from the Sample Tray/ Centrifuge, turn off power to Canister by switches on the Sample Tray/Centrifuge.

Entanglement with rotating device

Incubator Unit 1G

Verify visually through the Observation Window that Centrifuge is not rotating before opening the door of the Incubator Unit 1G.

Centrifuge When attaching/detaching the Canisters to/from the 1G Centrifuge, lock turntable of the Centrifuge.

Time Critical Operation N/A N/A

Dos and Don’ts Incubator Unit Do NOT touch inside Incubator Unit with bare hand to keep it clean.

Sample Tray,

Centrifuge

Be careful not to pinch fingers when releasing drawer locks of slide rails on Sample Tray/Centrifuge.

Difficulty in Ops N/A N/A

Possible Mis-Ops N/A N/A

<CBEF>

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Key Points for CERISE Operations (CB)

NOTE: Underlined key points have been covered by CB Advanced Training.

Category Equipment Key Points

Crew Safety

Crew entrapment Air-tight Chamber

When CB is in ‘Stowed Configuration’ fill gaps between the OC and Outer Chassis with Gap Protectors to prevent crew entrapment.

Crew Exposure to Broken Glass

Operation Chamber

Verify no broken glass in Operation Chamber before opening OC door.

Time Critical Operation N/A N/A

Dos and Don’ts Air-tight Chamber

Pull out/stow Air-tight Chamber slowly at about 10 cm per minute to avoid damage to bellows.

OC Camera Raise OC Camera (Lens and Flexible Arm) above the inner plate before stowing the Operation Chamber.

Difficulty in Ops N/A N/A

Possible Mis-Ops N/A N/A

<CB>

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Appendix

Appendix

1. Specification of Meas Exp Unit A

2. Schematic of Meas Exp Unit A

3. Photo of Meas Exp Unit A

4. Acronyms

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Specification of Meas Exp Unit A

Item Specification

Size 239 x 166 x 102 mm

Weight 1585 g

Power +15V, -15V

+12V (for future experiments)

Temp Sensor 20~45 deg C

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Schematic of Meas Exp Unit A

CBEFCircuit Board

Analog (A)

(0~5V)

Analog (B)

(0~5V)

Temperature Sensor (1)

Temperature Sensor (2)

+12V

+15V

-15V

NTSC

ShieldConnector for future

experiments

Jumper

SEL

Relay circuit

Relay circuit

Amplifier circuit

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Overview

Inside

Photo of Meas Exp Unit A

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Acronyms

CBCBEFMELFIMLTMMAOCRNAi

Clean BenchCell Biology Experiment FacilityMinus Eighty degree Celsius Laboratory Freezer for ISSMMA Laptop TerminalMicrogravity Measurement ApparatusOperation ChamberRibonucleic Acid Interference