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TESCAN and Leica Microsystems invite you to a 1-day workshop on
Cryo-Scanning Electron Microscopyin Biological Sciences
Cryo-scanning electron microscopy (Cryo-SEM) has become an established technique for capturing and observing biological samples close to their natural state. It is a method of choice, where the traditional sample preparation (e.g. critical point drying) causes unwanted changes in the sample structure. A Cryo-SEM workflow typically involves freezing of the sample using high pressure freezing. This step creates a time snapshot of the cellular processes with minimum artifacts. Frozen samples are then transferred to a cryo sputter coater, where they are coated with a conductive layer of metals or carbon. Finally, the samples are inserted into a SEM chamber equipped with a cryo-stage and observed under high vacuum conditions.
This introductory workshop will cover all parts of the whole process, from the sample preparation to the image capture. New users can get familiar with the cryoSEM technology, while the experienced users can discuss their applications with the company representatives.
You have two opportunities to attend the workshop.You can visit the workshop on:
April 9th or April 10th 2014
at TESCAN Applications Laboratories in Brno
Libušina třída 21, 623 00 Brno, Czech Republic
9:00 – 9:30 9:30 – 10:0010:00 – 10:30
10:30 – 11:00
11:00 – 12:0012:00 – 15:00
15:00 – 16:00
RegistrationScanning electron microscopy of biological specimens (TESCAN)
Cryo-Immobilization Techniques for Biological electron Microscopy (Leica Microsystems)
MAIA3: An UHR SEM for high resolution, low-kV imaging of bio samples(TESCAN)
LunchPractical demonstrations of EM HPM100, EM ACE600, EM VCT100 and MAIA3(Leica Microsystems, TESCAN)
Coffee & Sweets, Open discussion, Q & A
Preliminary program:
Workshop is free of charge, registration will be first-come first serve basis.
Please register via email to [email protected]
