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NI DUNGIGii thiu v curcuminIIIPhng php phn lp curcumin t curcuminoidCc phng php trch ly curcuminoidIIGii thiu chung:Thnh phn chnh ca curcuminoid mt cht trong c ngh

Tn ha hc ca n l(1E,6E)-1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadien-3,5-dion.CTPT: C21H22O6Cng thc cu to

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C mu vng

Tan trong du, ethanol, methanol, dichloromethane, acetone

Hu nh khng tan trong nc mi trng acid v trung tnh, tan trong mi trng kim.IGii thiu v curcumin2) Vai tr v ng dng curcumin:

Ti sao phi tch v tinh ch CurcuminIGii thiu v curcumin2) Vai tr v ng dng ca curcuminVai tr ca Curcumin:IGii thiu v curcumin2) Vai tr v ng dng curcumin

D dy, gan, tim mchTr hen suyn, ho, cm..Khng vimTiu ha tt Phng chng ung thPhng chng HIV/AIDS p daIGii thiu v curcumin1Cc phng php trch ly trc y2Cc phng php trch curcuminII Nguyn tc trch lyPhng php trch ly bng siu m 3

1 Nguyn tc trch lyCc phng php trch curcuminIICc phng php trch curcuminIICc phng php trch ly trc y2Chng ct li cun hi nc

`CurcuminTinh du ngh``Hi nc Nc nngu im:D thc hin, thit b n gin ( khng tbi ph tr), Sau chng ct ly b rn i gn, lng. Sau ly b rn i sy10PHNG PHP CHNG CT LI CUN BNG HI NC

X lC nghHi ncChng ct li cun hi ncCn rnTinh duLng, gnNc nngCn, rnSyCurcuminoidTrch ly bng dung mi hu c

`CurcuminTinh du ngh``Este du haEthanolu im:D thc hin, thit b n gin ( khng tbi ph tr), Sau chng ct ly b rn i gn, lng. Sau ly b rn i sy13X lC nghEther du haTrch lyCn rnTinh duTrch lyEthanolDung dch lngCurcuminoidB rnTrch ly bng soxhlet

`CurcuminTinh du ngh``Este du haEthanolu im:D thc hin, thit b n gin ( khng tbi ph tr), Sau chng ct ly b rn i gn, lng. Sau ly b rn i sy15

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Curcumin dB phn hyC inNhc im ca cc phng php:

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19IIIPhng php phn lp curcumin t curcuminoidNguyn liu: bt curcuminoid c chit sut t ngh vngQuy trnh phn lp:

Kt tinh liBt curcuminoidTinh thSc k ctCurcuminCurcumin tinh< 95 %H dung mi methanol ncThi gian: 24h, nhit : 5 o CIIIPhng php phn lp curcumin t curcuminoidKt tinh li: Mc ch:Loi b tp chtThu phn tinh th c hm lng Cur caoNguyn tc: Da trn tnh tan khc nhau ca cc cht trong dung mi theo nhit v tinh khit ca hn hp em kt tinh Dung mi s dng: Methanol, nc ctIIIPhng php phn lp curcumin t curcuminoid2. Sc k bn mng (TLC): Mc chTheo di qu trnh sc k ct

Xc nh h dung mi cho sc k ct

IIIPhng php phn lp curcumin t curcuminoidNguyn tc: Da trn s tng tc khc nhau gia cc thnh phn trong hn hp vi dung mi v pha tnh

Nguyn liu: Bt curcuminoid sau 3 ln kt tinhMethanol DicloromethaneCloroformAcetoneBn mng silica gel 60G, Merck2. Sc k bn mng (TLC): IIIPhng php phn lp curcumin t curcuminoidPht hin vt v tnh tr s Rf:

Hin hnh cc vt sau gii lyMu sc t nhinPhun thuc th trong dung mi thch hp n UV2. Sc k bn mng (TLC): IIIPhng php phn lp curcumin t curcuminoidPht hin vt v tnh tr s Rf:

Tnh Rf :

Nhn xt v nh gi cc vt trn mng mng chn dung mi chy bn mng ph hpChn dung mi ra gii ct sc k2. Sc k bn mng (TLC): IIIPhng php phn lp curcumin t curcuminoid

Cc my sc k bn mng:2. Sc k bn mng (TLC): IIIPhng php phn lp curcumin t curcuminoid3. Sc k ct: Mc ch: Tch, tinh ch Curcumin t curcuminoidNguyn tc: Da vo i lc khc nhau ca cc cht trong hn hp ban u i vi pha tnh trong ct v pha ng qua ct

Phng php thc hin:

Ct thy tinh hnh trPha tnh: Silica gel 60G, 0.063-0.200 mmPha ng: Kho st trc bng TLC v chn dung mi 100% CH2Cl2Mu: bt Curcuminoid kh

IIIPhng php phn lp curcumin t curcuminoidTin hnh

Chun bNhi ctChun b mu v cho mu vo ct sc kTrin khai ctThu v kim tra thnh phn mi phn on bng sc k bn mng

3. Sc k ct: IIIPhng php phn lp curcumin t curcuminoid My sc k ct:

3. Sc k ct: