• Microfluidic Devices • Hydrophobic Coatings• Oleophobic Coatings

Company Focus

• Family of devices for semen and micro-biologic cell counting

• Fixed cover with even chamber depth for great cell counting accuracy

• Compatible with computer assisted sperm analysis (CASA) instruments

• Manufactured from inert non-toxic materials that are safe for all cells, including delicate boar sperm.

MicroTool

MicroTool20 µm depth chambers with fixed coverslip

Small volume (2 µl or 4 µl) chambers

Affordable: Manufacturer to end-user direct pricing model

100% laser interferometry inspection process verifies chamber depth

Made in the USA since 1987

Used by many universities

Compatible with commercial CASA systems

Compatible with free open-source ImageJ CASA

Free ground shipping within the USA

Cytonix Slides and Other Analysis Methods

• Study conducted at the London Royal Veterinary College.

• Compare 6 methods for determining sperm concentration:

• Flow-Cytometry (Flow)• Hemocytometry (Hem)• Image-Analysis (Image)• Cytonix Chambers (MicroX)• Plate-Readers (Plate)• Spectrophotometry (Spec).

• The results show that Cytonix chambers showed good correlation (>0.8) with other cell counting methods

• Prathalingam NS, Holt WW, et al., J. Andrology, 27: 257-262, 2006

Sperm Mobility• Study conducted at the Wayne State University,

Michigan

• Sperm mobility and type of sperm movement can be assessed reliably in Cytonix counting chambers due to a unique coating in the devices.

• Cytonix Chambers are manufactured with a crystal clear coating to reduce cell adhesion to raw glass surfaces yielding excellent motility and low lateral head displacement of sperm

• Armant DR, Ellis MA, Fertil. Steril. 63:1128-30, 1995

Repeatability of Sperm Counts• Repeatability of sperm counting methods was studied by evaluating

the coefficients of Variation (CV) across 5 repeats:

• Flow-Cytometry (2.3%)• Spectrophotometer (4.1%)• Hemocytometer (7.8%)• Cytonix-Counters (10.7%)• Image-Analysis (14.9%)• Fluorescent Plate Reader (20.0%).

• Although Cytonix-Counters did not have the lowest CV, they have the advantage of allowing sperm motility assessment and sperm counting at the same time

• Prathalingam NS, Holt WW, et al., J. Andrology, 27: 257-262, 2006

Boar Sperm Pre-freeze, Post-Thaw and Post-Thaw Progressive Motility

• University of Illinois thesis studied boar sperm motility prior to freezing and after thaw using a Spermvision system and Cytonix 20 µm depth counting chambers

• The results showed average measures of ~73.1 x 109 cells per ejaculate, ~82% Pre-freeze Motility, ~55% Post-Thaw Motility and ~47% Post-Thaw Progressive Motility. The methods and results from this work showed good acceptable pregnancy rates and litter sizes from frozen-thawed boar sperm.

• Spencer KW, Thesis 2010, Department of Animal Sciences, University of Illinois, Urbana

Other Publications

Choi EK, Tsunekawa N, Kanai Y, Kurohmaru M. A new preparation protocol for measurement of testicular sperm production. J Reprod Dev. 2008;54:90-3.

Brazil C, Swan SH, Tollner CR, Treece C, Drobnis EZ, Wang C, Redmon JB, Overstreet JW. Quality control of laboratory methods for semen evaluation in a multicenter research study. J Androl. 2004;25:645-56.

Brazil C, Swan SH, Drobnis EZ, Liu F, Wang C, Redmon JB, Overstreet JW. Standardized methods for semen evaluation in a multicenter research study. J Androl. 2004;25:635-44

• Large of family of coatings with hydrophobic and oleophobic properties

• Millions of pipette tips coated per year

• Low retention prevents, water, oil, protein & DNA adhesion

• Other applications include circuit boards, medical devices, tubing, thixotropic inks, membranes, filters, etc.

FluoroPel

• Family of superhydrophobic coatings

• Contact angles to water over 150°

• Prevents water, ice and snow accumulation

• Radomes, microwave dishes, parabolic grid dishes, mobile radio antennas, passive reflectors and other telecommunication applications

FluoroThane

• Family of glass and metal coatings for smartphone and other touch devices

• Hydrophobic and oleophobic

• Extremely durable with >3000 steel wool abrasive rubs

• Contact angles to water over 110°

FluoroSyl

• Family of hydrophobic and oleophobic coatings

• Metal and plastic compatible

• UV curable and extremely durable

• Contact angles to water over 110°

FluorAcryl

• Family of surfactants

• Leveling agents

• Hydrophobic additives

• Oleophobic additives

• Applications include bowling lane conditioners and coatings to reduce freeze-thaw damage

FluorN

• 8,221,870 Articles comprising hydrophobic surfaces

• 8,168,264 Hydrophobic coating compositions, articles coated with said compositions, and processes for manufacturing same

• 7,579,056 Hydrophobic formulations and vessel surfaces comprising same

• 7,459,315 Miniaturized assembly and method of filling assembly

• 6,362,246 Porous surface compositions and methods of retaining biological samples on said surface

• 6,037,168 Microbiological assembly comprising resealable closure means

• 5,989,692 Porous surface for laboratory apparatus and laboratory apparatus having said surface

• 5,853,894 Laboratory vessel having hydrophobic coating and process for manufacturing same

• 5,503,803 Miniaturized biological assembly

• 4,911,782 Method for forming a miniaturized biological assembly

• 4,676,274 Capillary flow control

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