Metabolism

Course code: 13.1-WB-BT1-META

type of course: compulsory

Language of instruction: Polish

Director of course and lecturers

Director of course Name of lecturer

dr hab. Ewa Jaśkiewicz dr hab. Ewa Jaśkiewicz, dr Beata Machnicka

Type of course Number of hours per semester

Semester Course grade ECTS points

Intramural studies

3 Lecture 30 hours 4 credit assessment

Laboratory 30 hours 4 credit assessment

Extramural studies

Course characteristic

Aim of the course The main objectives of the course: - to be able understand and apply the methods used in metabolic studies, - to gain understanding of the localization, regulation and integration of metabolic pathways and

molecular basis of human metabolic diseases. Prerequisites Principial knowlege in physical chemistry: chemical equilibrium, entropy and laws of thermodynamics. Enzymes – principles and kinetics. Scope Principles of metabolism. Glycolysis and gluconeogenesis are regulated contrary. The citric acid cycle regulation. Regulation of oxidative phosphorylation. The pentosephosphate pathway regulation. Coordinated regulation of glycogen synthesis and breakdown. Fatty acids metabolism regulation. Regulation of biosynthesis of lipids and steroids. Amino acids biosynthesis is under allosteric regulation. Regulation of biosynthesis and degradation of nucleotides. Integration of mammalian metabolism: - key metabolites, - tissue specific metabolism, - glucose homeostasis during fasting , starvation and satiation.

Methods of education Lecture (in a form of multimedia presentation) - Practical method – laboratory classes with the use of Saccharomyces cerevisiae mutants as a model organism and microbiological equipment. Learning outcomes

Effect of the course

Knowledge, skills, competencies Area in the

natural sciences

Directional

P1A_W01 P1A_W04

K1A_W33 The student posseses the knowledge of metabolic pathways and understands its regulation and integration.

P1A_W07

P1A_W09 K1A_W34

The student possesses the knowledge of usage laboratory equipment and applies the ergonomic principles of work in

biochemical laboratory.

P1A_U01 P1A_U06

K1A_U10 The student plans and carries out simple experiments, is able to apply the research methods and technics.

P1A_U02 P1A_U03 P1A_U07

K1A_U02 The student is able to use the literature and other sources (e-learning) to interpret the results and draw the conclusions.

P1A_K05 P1A_K07

K1A_K09 The student applies self-study method and understands the need to master knowledge and skills in biochemistry.

P1A_K02 K1A_K02 The student can work in a group and organise work within a determined range.

Verification of learning outcomes and credit conditions The lectures- the test is conducted in the written form, it lasts 60 minutes and contains 30 open questions. 60% of the points out of 60 are required to get the pass mark credit. The laboratory classes: the attendance at classes, the laboratory experiments carried out correctly and the pass mark credit of 2 tests (the first 20 questions test, 60% of the points out of 20 are required to get the pass mark credit, the second 3 open questions test, 60% of the points out of 9 are required to get the pass mark credit.

Student workload The contact hours: - the lectures (30 hours)- the laboratory classes (30 hours)- the consultations (5 hours). The unassisted student work: the preparations for the laboratory classes (30 hours)- the examination preparations and the attendance at the examination test (30 hours + 1 hour). The total amount of 126 hours refer to 3 ECTS points. The determined student workload include: the workload of the direct assistance of the academic teacher: 30+30+5+1h =66 hours which refer to 2 ECTS points, the practical classes workload : 30+5+10h=45 hours which refer to1 ECTS points.

Literature

Recommended reading

Berg J, Tymoczko J L, Stryer L, Biochemia, wyd. V, PWN, 2005

Murray R K, Granner D K, Mayes P A, Rodwell V W, Biochemia Harpera, wyd V, Wydawnictwo

lekarskie PZWL, 2006

Hames B D, Hooper N M. Krótkie wykłady-biochemia, PWN, 2007