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Energy Harvesting Pathways
Glycolysis & Cellular Respiration
energy harvest
, storage
& transfe
rsFigure 7.1
energy transfers
•two ways to transfer metabolic energy from one molecule to another–as free energy during coupled exergonic/ endergonic reactions
–as “high energy” electrons during reduction/oxidation reactions
reduction reactions transfer energy
Figure 7.2
of course, some usable energy is lost in the transfer
NAD+ accepts reducing equivalents (H & e-)
Figure 7.4
(NADH+H+) + 1/2 O2 => NAD+ + H2O
G = -52.4 kcal·mol-1
NAD+/NADH shuttles reducing equivalentsFigure 7.3
retrieving energy from storage
•glucose is the most common metabolic fuel–other fuel molecules use the same catabolic pathway
•when glucose is completely oxidized (burned)C6 +6O2 => 6CO2+6H2O + energy G= -686 kcal/mol
•when glucose is oxidized metabolicallyC6 +6O2 => 6CO2+6H2O + energy
~ half of released energy is transferred to ATP
stages of
glucose
oxidation
Figure 7.5
retrieving energy from storage
•glucose is oxidized by a series of regulated metabolic pathways–glycolysis (cytoplasmic)•yields ATP, NADH & •two 3C pyruvates
–cellular respiration (mitochondrial)•converts pyruvate to CO2 & H2O, and•yields ATP, and •absolutely requires O2
fermentation:
partial oxidation
of glucose in the
absence of oxygen
OR,if O2 is
shortFigure 7.5
Cell Resp/Ferment LocationsTable 7.1
free energy changes duringglycoly
sisFigure 7.7
Investment, Isomerase,Harvest I,Harvest IIFigure 7.6
glycolysis
products:
NADH (2)ATP (2)pyruvate
(2)Figure 7.7
retrieving energy from storage
•glycolysis–a ten-step metabolic pathway –in the cytoplasm
•cellular respiration–NADH & pyruvate go to the mitochondrion•pyruvate is oxidized, and•decarboxylated–COOH functional group (carboxyl) is released as COO (CO2)
coenzyme A cycleFigure 7.8
citric acid
cycle,
tricarboxylic acid
(TCA) cycle,
Kreb’s cycleFigure 7.8
retrieving energy from storage
•pyruvate oxidation produces acetyl-CoA which enters the citric acid cycle
•2C acetate joins 4C oxaloacetate => 6C citric acid
•atoms are rearranged
•CO2 is released
•intermediates are oxidized•ATP is formed•more oxidation & rearrangement
final enzymatic disassembly
of glucose
by acyclic acetate burner
with energy capturing accessoriesFigure 7.8
energy yield of glycolysis
and citric acid
cycleFigure 7.9
retrieving energy from storage
•the major energy product of glycolysis and citric acid cycle is NADH
•the major metabolic energy demand is for ATP–citric acid cycle enzymes are in the mitochondrial matrix
–NADH reduces an enzymatic pathway on the inner mitochondrial membrane
fate of electrons from
glucoseFigure 7.10
change in free energy during
electron transport
Figure 7.11
electron transport proton pump
proton translocation during electron transport
Figure 7.12
retrieving energy from storage
•NADH drives electron transport•electron transport drives proton pumping
•proton pumping produces a transmembrane electrochemical gradient
•the phospholipid bilayer blocks diffusion of protons into the matrix
the ATP synthase proton channel relieves
the transmembrane proton gradient,
andthe proton gradient drives ATP
synthesis
chemiosmosis
Figure 7.12
a proton gradient
is sufficient to
generate ATP
Figure 7.13
retrieving energy from storage
•fermentation
–occurs when O2 is insufficient to drive cellular (aerobic) respiration
–IS NOT “anaerobic respiration”
–regenerates NAD+
lactic acid fermentation regenerates
NAD+
Figure 7.14
ethanolic fermentatio
n regenerates
NAD+
Figure 7.15
energy balance sheetFigure 7.16
interacting
metabolic
pathways
Figure 7.17
transamination forms an amino acidFigure 7.18
positive & negative feedback
coordinate the integrated
metabolic pathways
Figure 7.19
positive &
negative feedback control
glycolysis
Figure 7.20