Figure Legends for Supplemental Data:

Figure S1: Legend for Receptor Tyrosine Kinase Arrays. The location of the specific

RTKs and controls in duplicate are shown.

Figure S2: Receptor tyrosine kinase phosphorylation in melanoma cell lines. Phospho-

Receptor Tyrosine Kinase arrays with duplicate spots of capture antibodies for 42

different receptors are shown with pairs of (+) control spots at the corners (boxed) for the

VMM609 and VMM425 melanoma cell lines for Experiments A and B. Spearman

correlation plots for the VMM609 and VMM425 melanoma cell lines where the log of

the value for the RTK is divided by the log of the value for the control for both the X axis

(value from experiment A) and Y axis (value from experiment B) are shown.

Figure S3: Receptor tyrosine kinase phosphorylation in melanoma tissues. Phospho-

Receptor Tyrosine Kinase arrays with duplicate spots of capture antibodies for 42

different receptors are shown with pairs of (+) control spots at the corners (boxed) for the

VMM609T, VMM425T, VMM5AT, VMM17T, and VMM39T melanoma tissues.

Spearman correlation plots for the VMM609T and VMM425T melanoma tissues where

the log of the value for the RTK is divided by the log of the value for the control for both

the X axis (value from experiment A) and Y axis (value from experiment B) are shown.

Figure S4: Receptor tyrosine kinase phosphorylation in melanoma cell lines. Phospho-

Receptor Tyrosine Kinase arrays with duplicate spots of capture antibodies for 42

different receptors are shown with pairs of (+) control spots at the corners (boxed) for the

14 melanoma cell lines shown.

Figure S5: Receptor tyrosine kinase phosphorylation in serum-starved melanoma cells.

Phospho-Receptor Tyrosine Kinase arrays with duplicate spots of capture antibodies for

42 different receptors are shown with pairs of (+) control spots at the corners (boxed) for

the 6 melanoma cell lines after 18 hrs of serum starvation are shown.

Figure S6: Detection of P-HGFR in human melanoma tumors and cells. A, Western

blot analysis of phospho-HGFR and total HGFR on 8 different tumor samples are shown.

GAPDH is blotted as a loading control. B, Western blot analysis of phospho-HGFR and

total HGFR on the DM122 and HT144 melanoma cell lines. GAPDH is blotted as a

loading control.

Figure S7: Anti-MSP antibody compared to IgG isotype control does not inhibit

proliferation of human melanoma cell lines. A-D, Four melanoma cell line dose response

curves are shown. The percent change in cell number versus concentration in ug/ml of

antibody is shown in a cell proliferation assay using Cell Titer-Glo starting with 1,000

cells/well. Cells were cultured in media plus serum with no treatment (hatched bar), plus

an IgG control antibody (white bars), and an anti-MSP antibody (black bars) at

concentrations ranging from 0.01 to 10 ug/ml. Error bars represent standard deviations

from three independent experiments.

Table 1: Raw Integrated Intensities for RTK arrays from Cell Lines from Figures 1 and S2 VMM609 –

expt A VMM917 – expt A

VMM425 – expt A

VMM609 – expt B

VMM917 – expt B

VMM425 – expt B

AxI 1648481 3170109 0 10046444 6315430 691828 c-Ret 1655998 1042930 131914 3046602 2821810 1360603 Dtk 1505017 299485 1443786 3550364 1609408 2562073 EGF R 339535 10116306 514883 2265987 14332928 366324 EphA1 963930 182201 432143 3739514 532072 726762 EphA2 646419 948886 483864 2194020 1356207 1006345 EphA3 403133 63085 290692 1532273 454264 160209 EphA4 5600429 1784777 1449693 5654485 8593519 6859097 EphA6 560431 4042888 202217 895065 844973 22895 EphA7 1292772 671034 329445 1954622 1549585 412261 EphB1 290560 456858 284136 2296008 622769 87623 EphB2 1129198 1157217 592951 6238681 1889291 893122 EphB4 627675 600560 124576 2772443 1084803 398957 EphB6 168342 191654 405407 2398212 815373 148846 ErbB2 2450130 1142315 1445242 3257078 2759719 2421541 ErbB3 12419869 3100059 1775076 11218307 6794077 2295115 ErbB4 1841183 1131072 1493396 6221643 2674150 1818869 FGF R1 45911 86150 173467 514295 460755 326401 FGF R2a 1362598 705444 170951 3771775 1125994 1155342 FGF R3 10618523 11219705 4646246 20529686 13586541 12193896 FGF R4 456113 227389 0 1660425 718421 0 Flt-3 4251715 2926342 2698156 8754216 7004358 7321112 Goat IgG 394197 387443 297977 4751920 933695 552889 HGF R 197026 14420104 5754177 2591531 19822436 7376702 IGF-I R 11501059 7877367 5443114 22062954 15766333 12486466 Insulin R 3337292 2444205 4533188 10734687 5139720 9162857 M-CSF R 923792 432851 859306 4450889 946552 2455773 Mer 1991309 1714134 881088 4473114 2704062 924658 Mouse IgG1 305572 315478 130513 1492921 920050 130082 Mouse IgG2a 804042 302280 209385 1910810 522031 654098 Mouse IgG2b 577339 390411 230387 2229734 841193 0 MSP R 4071467 2666835 1168540 6152235 5407227 2140776 MuSK 571851 761181 220324 2919338 440732 903116 PBS 0 0 433233 325034 358889 359514 PDGF Ra 268432 319612 0 1912506 2132573 199398 PDGF Rb 454793 787746 120742 1526934 1204002 217101 PY-control 710812 1997062 2118409 4043078 7316566 959810 PY-control 872193 3196364 1719660 6001186 6846493 1197267 PY-control 920475 2110826 7226342 2357269 5615001 2399058 PY-control 1048333 3106628 2022306 4987150 7387688 1302142 ROR1 250426 178014 0 914972 511631 0 ROR2 304047 689672 153024 1183052 782869 395913 SCF R 400801 255152 632903 1334625 491641 1273526 Tie-1 785790 275744 667795 2820587 864242 700010 Tie-2 1105375 1456511 375640 6034288 3614829 1273228 TrkA 648149 253749 343053 2776956 1296938 220856 TrkB 1563919 2132261 784307 2589305 4634739 578261 TrkC 511477 407084 573890 1658451 1359343 716035 VEGF R1 37483 357692 0 797083 1073077 250480 VEGF R2 875590 2332482 25974 4097625 3718891 465523 VEGF R3 2406372 1027795 1044552 6759450 1470527 1718118

Table 2: Raw Integrated Intensities for RTK arrays from Tissue from Figures 1 and S3

VMM609T VMM917T VMM425T VMM5AT VMM17T VMM39T AxI 291730 852898 953558 2296 546 4059 c-Ret 324565 835736 1475072 1442 1349 6969 Dtk 4434752 13114393 9703104 27887 24280 13854 EGF R 5372299 5584939 7133198 20323 4947 7204 EphA1 506012 2416189 3189126 4198 271 43951 EphA2 376290 2266488 1897668 3164 1511 74037 EphA3 386593 1129639 857730 4434 1649 68250 EphA4 272666 1306680 861706 3545 336 3072 EphA6 0 80043 314958 1762 360 2555 EphA7 223884 370103 1881110 3469 176 7426 EphB1 168267 378078 1783946 2326 1944 14498 EphB2 1550500 2705108 6009993 23139 4440 4908 EphB4 294489 1262622 1998045 4111 1582 1536 EphB6 610836 2310969 1951621 4440 1146 3702 ErbB2 378519 396937 2262564 5920 2179 1713 ErbB3 63006 1260963 3211691 4400 1297 1599 ErbB4 917697 3101677 2733159 5855 425 2391 FGF R1 257183 610048 1435971 1352 1132 1553 FGF R2a 835628 1893642 2067164 5003 578 6208 FGF R3 413260 1501633 1780621 6442 2569 15732 FGF R4 180084 646928 1119610 3939 1928 1342 Flt-3 373799 1220746 1319178 4864 1306 2197 Goat IgG 368533 1496424 1592334 2479 1692 11396 HGF R 191301 309445 1376131 1322 786 4152 IGF-I R 844450 2107142 2626889 4304 2486 1045 Insulin R 2261589 2666374 3137695 4881 2344 1114 M-CSF R 3159011 6219941 3992995 7659 2155 4116 Mer 586238 1137802 3124134 5723 1212 4192 Mouse IgG1 539387 1466963 1552539 4384 1160 1711 Mouse IgG2a 790015 1220288 2064896 3632 1735 1422 Mouse IgG2b 278866 893406 1391403 2238 2099 3429 MSP R 1078114 2471788 8490791 20168 3296 25898 MuSK 242757 689261 828417 3202 1322 14770 PBS 0 52304 317647 720 162 8053 PDGF Ra 721726 719647 1616752 3334 822 4273 PDGF Rb 3272997 2541595 3570848 4429 1406 5990 PY-control 5469694 10533791 3053262 80676 109913 83531 PY-control 6097668 14757254 2397066 109621 133083 88296 PY-control 6171996 16496853 2875615 80998 109209 95222 PY-control 9461680 10913053 3077111 101050 131796 86365 ROR1 515127 4061676 3717385 5371 5360 9876 ROR2 493116 2791728 3623305 9224 2191 5555 SCF R 18466 918636 893395 1774 799 2994 Tie-1 557083 2509165 3097252 8519 2326 2490 Tie-2 50545 542527 2285464 9708 889 38864 TrkA 347518 625143 2019582 4410 331 37939 TrkB 314382 1151256 3176627 7326 699 25322 TrkC 178452 566606 1255564 3680 868 38171 VEGF R1 286317 1611319 1358707 2093 1069 17305 VEGF R2 543168 1990235 1776605 1772 1134 9178 VEGF R3 574142 6220864 2059833 6371 1585 13529

Table 3A: Raw Integrated Intensities for RTK arrays from Cell Lines from Figure S4

HT144 VMM5A DM331 SKMel24 VMM1 VMM17 VMM15 AxI 9019 10357 112243 4951 5173 3829 5740 c-Ret 5952 2684 67869 3455 645 609 779 Dtk 3336 7413 84037 3237 2372 1821 1879 EGF R 1969 2059 67328 5617 3711 4205 11326 EphA1 2698 848 57386 3269 1365 1288 1938 EphA2 1000 474 58970 1150 2112 1572 904 EphA3 3580 5574 68557 1056 1368 2101 848 EphA4 4046 3280 67252 2178 1653 2512 4151 EphA6 1923 950 56931 0 674 1032 1250 EphA7 1263 1702 57259 8550 804 456 2354 EphB1 2028 1212 70285 6847 669 1103 1350 EphB2 4054 2732 62697 9077 1490 4917 3243 EphB4 4372 2008 58164 2053 952 3487 2178 EphB6 3809 915 66788 4429 743 2070 2443 ErbB2 3961 3311 70274 13508 4019 577 7246 ErbB3 15227 20997 89892 20125 17991 15528 27708 ErbB4 10161 9217 77973 5805 2928 4616 3405 FGF R1 3305 2247 93833 1251 1577 2019 1538 FGF R2a 3905 3431 83989 2247 1686 654 2617 FGF R3 16086 22083 145063 8629 17792 19063 9636 FGF R4 4953 2114 97513 686 607 732 2733 Flt-3 1728 4196 47267 2254 823 2931 2118 Goat IgG 1668 1896 63255 266 779 812 1837 HGF R 2092 9273 107446 12511 5557 11929 11322 IGF-I R 14976 17224 80969 5740 24367 17653 35673 Insulin R 10210 4960 92082 1574 16498 16366 15921 M-CSF R 6970 3496 85643 4771 1703 2279 2555 Mer 1969 2059 67328 5617 3711 4205 11326 Mouse IgG1 1638 625 63546 3457 717 854 1244 Mouse IgG2a 5293 1771 53792 631 1087 1426 2255 Mouse IgG2b 4314 1186 64615 617 557 234 1075 MSP R 5240 8410 83050 7042 2264 2315 4515 MuSK 4611 2566 55904 1600 1288 1121 1253 PBS 1665 5008 86326 575 1186 836 2700 PDGF Ra 1992 2819 73594 3028 1030 1406 424 PDGF Rb 3828 4150 73564 3667 1139 1117 625 PY-control 4824 14417 73648 6171 4562 10226 15446 PY-control 6106 11665 156662 4770 4820 8114 10841 PY-control 11037 21374 110021 9028 5461 8435 11018 PY-control 12005 19709 137786 11410 5988 7569 11600 ROR1 3128 1743 56842 1598 901 1513 2523 ROR2 6387 6745 75718 1833 1293 1787 1869 SCF R 3703 2422 63521 2242 778 917 2047 Tie-1 4295 2600 65497 2020 977 2083 1527 Tie-2 3249 2849 63748 8550 1267 950 2307 TrkA 825 1132 74289 6349 237 953 1610 TrkB 754 1257 65451 6105 803 927 1360 TrkC 707 1629 69478 3051 860 1363 1294 VEGF R1 3284 1951 69341 1662 1245 1984 535 VEGF R2 3090 2030 45638 781 1003 259 1418 VEGF R3 6164 11500 54900 3005 3004 2983 5475

Table 3B: Raw Integrated Intensities for RTK arrays from Cell Lines from Figure S4 (continued)

VMM14 DM6 DM13 DM93 VMM39 VMM18 DM122 AxI 4518 4154 3526 0 0 2806 2411 c-Ret 2585 1683 3701 1162 547 0 2492 Dtk 3521 4365 1479 566 371 3857 1063 EGF R 3011 2013 3292 1257 0 20341 2159 EphA1 841 2376 3266 0 625 4067 2319 EphA2 2800 1006 3784 221 0 1341 1333 EphA3 2222 0 3210 0 0 0 0 EphA4 3747 28607 35734 6668 9705 2504 2376 EphA6 1053 2473 2753 1183 838 1018 1352 EphA7 1779 1842 4003 6754 8804 3947 1337 EphB1 2435 1464 0 791 998 2643 428 EphB2 2985 758 1538 3926 9644 746 1097 EphB4 1409 2052 0 787 920 1225 438 EphB6 807 4074 593 1061 186 1867 0 ErbB2 5972 7852 4194 17139 1969 20347 17974 ErbB3 5512 57220 46280 68020 66338 56447 78291 ErbB4 5962 25094 10817 3 445 1637 2365 FGF R1 2068 1292 226 0 0 0 454 FGF R2a 1775 3535 647 0 0 2777 0 FGF R3 8146 65131 84654 64173 63806 39315 49225 FGF R4 2344 1237 2764 0 0 1688 0 Flt-3 1701 10967 9586 22278 15988 7542 15110 Goat IgG 1342 2000 3953 0 607 3065 517 HGF R 8376 15476 7694 12994 2665 17046 57467 IGF-I R 6834 48430 37873 49880 53538 75657 70607 Insulin R 7527 34341 42773 43896 0 77664 66754 M-CSF R 1917 1535 3753 340 0 121 728 Mer 3011 27510 25072 6817 15544 7326 11462 Mouse IgG1 210 934 2244 1143 0 1263 0 Mouse IgG2a 252 4489 818 1060 0 1282 0 Mouse IgG2b 952 1300 0 0 0 2610 1433 MSP R 5711 45298 27595 3456 1451 7492 6208 MuSK 485 1770 1825 312 0 2590 2230 PBS 1592 212 744 0 0 0 2310 PDGF Ra 1213 0 105 0 789 1673 1440 PDGF Rb 1367 1548 2493 1112 941 4353 2088 PY-control 6257 9960 6195 4036 7629 12983 19841 PY-control 6413 14162 8558 3933 8471 14519 21977 PY-control 7599 3335 9523 5881 2910 17070 16759 PY-control 4643 8469 15526 6344 5513 13586 18379 ROR1 1657 3343 1179 1093 0 0 0 ROR2 3187 2302 3869 491 392 1309 317 SCF R 817 1152 4236 152 137 881 96 Tie-1 3353 3987 2750 0 0 2786 1869 Tie-2 2410 4577 12594 944 6305 381 2094 TrkA 783 3064 3767 659 508 2143 2687 TrkB 1698 0 2761 839 700 4000 6348 TrkC 790 8531 15206 0 15636 1243 2454 VEGF R1 457 784 0 0 0 875 402 VEGF R2 1092 0 6742 1240 0 16180 0 VEGF R3 2476 28700 18621 3734 6504 8685 12873

Table 4: Raw Integrated Intensities for RTK arrays from Serum Starved Cell Lines from Figure S5

VMM39 VMM1 VMM5A VMM17 VMM609 VMM425 VMM917 AxI 1085 7570 1745498 2112791 4914027 687275 2504137 c-Ret 2131 1097 5539068 1758035 3763970 0 1787186 Dtk 1674 6870 2647100 713753 7701946 4053242 1848851 EGF R 871 2206 156975 506822 481350 667330 6171551 EphA1 2132 1220 1412288 724410 1277142 444352 0 EphA2 1006 3478 4427521 2510748 1513728 2822163 3630881 EphA3 4952 1077 1802658 1272326 1355971 107175 780097 EphA4 2635 3551 3384779 2402415 12607518 1689148 2823379 EphA6 452 428 0 15342 1014389 612341 306395 EphA7 2327 308 455436 214967 976393 172486 491066 EphB1 1882 3487 394328 4398069 690311 716617 5100097 EphB2 2356 1673 657277 2179573 6027137 666983 1920189 EphB4 1344 1663 2866399 1324101 1047329 643029 598075 EphB6 703 1356 1011927 656879 1221818 169692 709724 ErbB2 6946 2690 498495 194256 3219426 0 1326277 ErbB3 8577 1892 3748782 1807533 16699390 517774 1638115 ErbB4 28354 24718 6025431 12971027 8297201 2570289 13094855 FGF R1 1279 1987 1839056 661603 3176161 510445 350086 FGF R2a 1146 1421 2490076 1188576 3025206 488668 469664 FGF R3 5254 2792 20079153 17150427 16076653 7523928 9968286 FGF R4 1958 951 490470 9524 479665 79404 290867 Flt-3 4568 1954 459133 713211 1820554 834993 1126615 Goat IgG 2748 1586 2208201 167037 2547033 813998 415591 HGF R 4567 14960 18691095 11070215 830255 22389605 16544720 IGF-I R 46378 37951 22069473 20253089 17819365 13258183 12470459 Insulin R 1665 29440 378421 22099465 14706507 19012725 11220430 M-CSF R 2948 2604 2981975 2146875 2586832 1985100 885266 Mer 4059 7611 2956920 4734607 7211354 962612 4703361 Mouse IgG1 1056 1598 414367 136716 0 0 41087 Mouse IgG2a 2760 1835 2847107 3213293 1507425 418620 1956447 Mouse IgG2b 2128 1555 800255 293766 36721 0 204968 MSP R 3703 12375 4914478 13821242 8669331 2907038 12142929 MuSK 2103 602 1917610 763342 2720740 213788 938647 PBS 3345 4427 0 0 0 175707 94681 PDGF Ra 695 1313 116870 6430746 2575558 236303 12141774 PDGF Rb 1463 2287 285855 1357645 1332175 257737 658164 PY-control 15433 4435 663758 581367 6934017 2039654 735844 PY-control 18031 9021 753931 793331 2881405 3495299 1618723 PY-control 18457 6181 328528 1005339 2693359 2434589 965167 PY-control 19252 4974 618803 1841665 5204716 2452440 0 ROR1 2468 733 0 0 186003 0 0 ROR2 1874 2815 2071101 1617640 1846271 0 3417152 SCF R 1355 1985 2278587 717337 1184951 8642117 254201 Tie-1 1228 5104 1423903 554059 2588116 0 1493843 Tie-2 1315 2899 1605108 420297 4194997 0 755165 TrkA 4906 1130 496065 466728 2540676 252580 396056 TrkB 5113 7215 1722206 1532000 2401093 3835135 3707083 TrkC 1936 644 245870 138908 972984 59077 38473 VEGF R1 1504 1945 0 998332 970154 181355 492289 VEGF R2 1672 461 3647668 1035648 1804851 105813 3650695 VEGF R3 3367 7154 15784538 12225308 11129484 19206639 9514810