FORENSIC CHEMISTRY FORENSIC CHEMISTRY

AS SCIENTIFIC AND EDUCATIONAL AS SCIENTIFIC AND EDUCATIONAL

SPECIALISATION SPECIALISATION

AT THE JAGIELLONIAN UNIVERSITYAT THE JAGIELLONIAN UNIVERSITY

Paweł Kościelniak

JAGIELLONIAN UNIVERSITY, Faculty of Chemistry

INSTITUTE OF FORENSIC RESEARCH

Cracow, Poland

Jagiellonian University (1364)

Faculty of Chemistry

191950-5250-52 19291929

Institute of Forensic Research

DEPARTMENT OFANALYTICAL CHEMISTRY

(25)

GROUP OF TOXICOLOGICAL

AND PHARMACEUTICAL

ANALYSIS(5)

GROUP OF ANALYTICAL

FLOW TECHNIQUES

(11)

GROUP OF CHEMOMETRICS

(3)

LABORATORY FOR

FORENSIC CHEMISTRY

LABORATORY FOR FORENSIC CHEMISTRY

- system for capillary electrophoresis (PrinCe 550 CSD, Prince)

- HPLC chromatograph (LaChrom, Merck)

- GC chromatograph (3900, Varian)

- atomic fluorescence spectrometer with hydride generation (AFS 230, Beijing Haiguang Instrument Co.)

- system for microwave digestion (MARS 5X, CEM),

- audiovisual equipment (video-projectors, digital photo-cameras, video-camera, video-recorders)

founded in 2000

Sem. SubjectNo. of hours

lectures classes seminars total

VI

Electronics and automatics 30 30

Optional 60 60

Total 90 90

VII

Electronics and automatics 60 60

Advanced analytical chemistry 45 45

Chemometrics and biometrics 30 15 45

Analysis in forensic chemistry 15 15

Radiometric analysis 30 30

Toxicology 30 30

Flow analysis 15 15

Optional 60 60

Total 225 60 15 300

Sem. SubjectNo. of hours

lectures classes seminars total

VIII

Radiometric analysis   45   45

Forensic toxicology   60 15 75

Criminalistics 15 60 15 90

Low aspects of the forensic expert work 15   15

Student’s report   30 30

Optional 60   60

Total 100 100 100 315

IX

i

X

Laboratory for master thesis   450 450

Seminar for master thesis     60 60

Clinical toxicology 15   15

GLP 15   15

Optional 60   60

Total 90 450 60 600

No. Subject Place

1 Toxicological analysis of drugs IFR

2 Ethanol- selected toxicological and analytical aspects IFR

3 Application of GC/MS method to analysis of drugs in biological materials IFR

4 Determination of inorganic toxicants in biological material IFR

5 Chemical examinations of car paints IFR

6 Chemical examinations of fibers IFR

7 Chemical examinations of gun-shot residuals IFR

8 Chemical examination of inks by TLC IFR

9 Chemical analysis of inks by CE JU

10 Determination of Se and As in biological materials by AFS-HG JU

11 Comparative examination of psychotropic drugs by HPLC and CE JU

12 Profiling of drugs – physicochemical and chemometric examination JU

LABORATORY CLASSES

SubjectAnalytical method

FCh JU IFR

Determination of selected elements at normal levels in biological materials ICP/OES, AAS AAS

Development of novel techniques for preparation of biological samples HPLC, CE

Development of analytical procedures for determination of psychotropic drugs HPLC, CE

Development of analytical procedures for determination of selenium and arsenic in biological materials AFS

Application of Py-GCMS to analysis of criminalistic traces Py-GCMS

Identification and discrimination of paint traces chemometric

methods IR

Examination of questioned documents on the basis of identification of chemical composition of inks CE, LIBS

RESERCH SUBJECTS

PUBLICATIONS

M. Zadrożna, M. Gawlik, B. Nowak, A. Marcinek, H. Mrowiec , S. Walas, R. Wietecha-Posłuszny, P. Zagrodzki, „Antioxidants Activities and Concentration of Selenium, Zinc and Copper in Preterm and IUGR Human Placentas”, J. Trace Elem. Med. Biol., 23, 144-148 (2009).

M. Szafarska, M. Woźniakiewicz, M. Pilch, J. Zięba-Palus, P. Kościelniak, “Computer Analysis of ATR-FTIR Spectra of Paint Samples for Forensic Purposes”, J. Molec. Struct., 924–926, 504–513 (2009).

B. Trzcińska, J. Zięba-Palus, P. Kościelniak, "Application of Microspectrometry in Visible Range to Differentiation of Car Solid Paints for

Forensic Purposes", J. Molec. Struct., 924–926, 393–399 (2009).

R. Wietecha-Posłuszny, J. Dobrowolska, P. Kościelniak, P. Zagrodzki, „Determination of Selenium as a Biomarker of Thyroid Cancer by HG-AFS Method”, Acta Chim. Slov., 56, 441-446 (2009).

J. M. Milczarek, M. Dziadosz, J. Zięba-Palus, “Way to Distinguish Car Paint Traces Based on Epoxy Primer Layers Analysis by Pyrolysis - Gas Chromatography - Mass Spectrometry (Py-GC/MS)”, Chem. Anal., 54, 173-185 (2009.

K. Madej, „Microwave-assisted and Cloud-point Extraction in Dtermination of Drugs and Other Bioactive Compounds”, Trends in

Analytical Chemistry, 28, 436-446 (2009). K. Madej, A. Biedroń, A. Garbacik, „Study of Separation and Extraction Conditions for Five Neuroleptic Drugs by an LLE-HPLC-DAD

Method in Human Plasma”, Journal of Liquid Chromatography & Related Technologies, 32, 3025-3037 (2009).

E. Florek, E. Ignatowicz, W. Piekoszewski, “Effect of Pregnancy and Tobacco Smoke on the Antioxidant Activity of Rutin in an Animal Model”, Pharmacological Reports, 61, 935-940 (2009).

M. Woźniakiewicz, J. Kuczara, P. Kościelniak, „Determination of Fluoxetine in Blood Samples by High-performance Liquid Chromatography Using a Derivatization Reagent”, Chem. Anal. (Warsaw), 54, 667-677 (2009).

R. Wietecha-Posłuszny, T. Lech, P. Kościelniak, “Application of Three Spectrometric Methods to Total Selenium Determination in Post-

mortem Material in a Case of Acute Selenium Compound Poisoning”, J. Forensic Sci., in print. M. Szafarska, R. Wietecha-Posłuszny, M. Woźniakiewicz, C. Hughes, P. Kościelniak, „Influence of Storage Conditions on Ageing of Colour

Dye-Based Inkjet Printing Inks”, Problems of Forensic Sciences, in print

Ph.D. STUDYStudent Subject Defense

Renata Wietecha-Posłuszny Development of the analytical procedure for the selenium determination in human tissues by the AFS-HG technique

2004

Rafał Borusiewicz Examination of arson traces 2004

Joanna Mania Examination of inks by capillary electrophoresis 2005

Michał Woźniakiewicz Analysis of psychotropic drugs by HPLC and CE 2008

Justyna Dobrowolska Elemental analysis of biological materials 2008

Jakub Mielczarek Classification and discrimination of car paint traces 2008

Justyna Dobrowolska Elemental analysis of selected human and animal tissues with toxicological and clinical respects

2009

Małgorzata Szafarska Development of the method for discrimination of printer inks by capillary electrophoresis for criminalistic purposes

2010

Jagoda Majcherczyk Influence of tobacco smoke on pharmacokinetics of citalopram 2011

Aneta Garbacik Novel methods for preparation of biological samples to toxicological analysis 2011

Danuta Dudek-Adamska Trace elements in biological materials 2011

Agnieszka Biedroń Analytical and toxicological examination of selected drugs in biological materials

2012

Paweł Knihnicki Determination of psychotropic drugs in saliva by capillary electroforesis 2012

Tomasz Lachowicz Py-GCMS analysis as a tool in forensic examination of criminalistic traces

2012

Agnieszka Solarz Analysis of printer inks by capillary electrophoresis for criminalistic purposes 2013

PROJECTS

TEMPUS program, project „Transformation of University Chemistry Studies at the Jagiellonian University” (TRUCS); 1997-2000

TEMPUS program, project „National Training Program for Forensic Science Experts” (NATFOSE); 1998-2001

LEONARDO DA VINCI program, project „Vocational Training System in Forensic Sciences - Exchange of Educational Experiences” (VITSFOS-E); 2001-2003

NATO program, project “Sensors for Chemical Weapons Detection Based on Molecularly Imprinted Polymers”; 2004-2005

TEMPUS program, project „Education System in Forensic Sciences for the Republic of Macedonia”, (EDUFORMAK); 2007-2010

Government Grant, project „Application of the Capillary Electrophoresis as a Tool in Criminalistic Examination of Ink Printed Documents”; 2007-2010

Operational Programme 'Innovative Economy', project „Mikro and Nano-systems in Chemistry and Clinical Diagnostics” (MNS-DIAG); 2009-2013

Government Grant, project „The Use of Modern Analytical Instrumentation for Purpose of the Fight Against Crime”; 2010-2013

Laser-induced breakdown spectrometer ((LIBS)

Laser-induced breakdown spectroscopy (LIBS) is a type of atomic emission spectroscopy, which uses a highly energetic laser pulse as the excitation source. The laser is focused to

form a plasma, which atomizes and excites samples.

Price: ~ 45 000 EUR

PLASMA EMMISIONPULSED LASER (1064 nm)

PLASMA PLUME

SAMPLE