Gary Young

Evolution of the Ultimate Antiseptic

The Story of Thieves, Triple 0 , and Inner Defense

Early History of “Thieves”

• During the 14th Century plage (known as the Black Death) the story was told of a band of thieves who robbed the corpses of plague victims.

• When captured, the bandits revealed the secret of their immunity from contracting the disease in exchange for leniency.

• The told of a concoction that included clove and rosemary that afforded them protection from contracting diseases.

BACTERIA (gram negative/positive) FUNGI/MOLDS VIRUSES

Acinetobacter calcoacetica5,8 Micrococcus spp6 Aspergillus flavus16 Herpes virus I and II 4

Actinobacillus actinnomycetemcomitans2 Morazella sp.5,8 Aspergillus niger19

Actinomyces viscosus2 Mycobacterium phlei6,19 Aspergillus parasiticus7,11

Aeromonas hydrophila5,8 Peptostreptococcus anaerobius2,3 Candida albicans19

Alcaligenes faecalis5,8 Photobacterium phosphoreum14 Colletotrichum musae12

Bacillus sp.18 Porphyromonas gingivalis1,2 Fusarium moniliforme11

Bacillus subtilis5,6,8,19 Prevotella buccae3 Fusarium proliferatum12

Beneckea natriegens5,8 Prevotella intermedia1,3 Lasiodiplodia theobromae12 1 Cai and Wu, 1996

Brevibacterium linens5,8 Prevotella nigrescens2 Mucor sp.19 2 Shapiro et al., 1994

Brochothrix thermosphacta5,8,9 Prevotella oris3 Penicillium chrysogenum19 3 Didry et al., 1994

Campylobacter jejuni10 Proteus vulgaris5,8 Penicillium citrinum17 4 Benencia et al., 2000

Carnobacterium piscicola9 Pseudonomas aeruginosa5,8,19 Penicillium expansum15 5 Dorman and Deans, 2000

Citrobacter freundii5,8 Pseudonomas fluorescens6,9 Rhizopus sp.19 6 Farag et al. 1989

Clostridium sporogenes5,8 Saccharomyces cerevisiae6 7 Farag et al. 1989

Eikenella corrodens2 Salmonella enterica10 8 Lis-Balchin et al., 1997

Enterobacter sp. 18 Salmonella pullorum5,8 9 Ouattara et al., 1997

Enterobacter aerogenes5,8 Sarcima spp.6 10 Friedman et al., 2002

Enterococcus faecalis5 Selenomonas artemidis2 11 Juglal et al., 2002

Erwinia carotovora5,8 Serratia liquefaciens9 12 Ranasinghe et al., 2002

Escherichia coli5,6,8,19 Serratia marcescens5,6,8 13 Smith-Palmer et al., 2002

Escherichia coli 0157:H710 Staphylococcus sp.18 14 Mejlholm and Dalgaard, 2002

Flavobacterium suaveolens5,8 Staphylococcus aureus5,6,8,19 15 Venturini et al., 2002

Klebsiella pneumoniae5,8 Streptococcus milleri3 16 Lopez-Malo et al., 2002

Lactobacillus curvatus9 Streptococcus mitis3 17 Vazques et al., 2001

Lactobacillus plantarum5,8 Streptococcus mutans3 18 Moleyar and Narasimham, 1992

Lactobacillus sake9 Streptococcus pneumoniae20 19 Yousef and Taw il, 1980

Leuconostoc cremoris5,8 Streptococcus sanguis2,3 20 Horne et al., 2001

Listeria monocytogenes10,13 Streptococcus sobrinus2 21 Chao, Young, and Oberg, 2000

Micrococcus sp.18 Treponema vincentii2

Micrococcus luteus5,8 Yersinia enterocolitica5,8

Clove vs PathogensClinical studies have shown that clove and it’s primary phenol, eugenol, kills:

–60 different species of Gram Negative and Postive Bacteria

–13 different fungi

–2 different viruses

Powerful Antifungal Action

• Clove oil at very small levels (0.06%) totally inhibited toxic mold and aflaxtoxin production– Dr. Radwan Farag

Antiviral Effects of Eugenol

• Eugenol strongly inhibits growth of Herpes Simplex 1 and II in several clinical studies

• Eugenol significantly delayed the onset of herpetic lesions

Tiny amounts of cinnamon bark EO decimate E coli in only 2 hours

• Only 0.025% of cinnamon EO reduced toxic E. Coli 0157:H7 counts from 10,000,000 to

31,000 CFU.

Journal quotes

• According to the International Journal of Food Microbiology, the essential oils of clove, cinnamon bark, and thyme had the most powerful antimicrobial effects of 25 different essential oils tested

• Showed inhibitory affects against 23 different strains of bacteria.

                                      

Creation of Thieves

• Thieves essential oil– Clove– Lemon– Cinnamon– Eucalyptus radiata– Rosemary

• In 1997 Sue Chao tested Thieves EO at Weber State University for its potent antimicrobial effects

When diffused, Thieves EO Blend decimates 3 of the most common pathogenic bacteria

–Micrococcus leteus Common gram-positive bacterium

–Pseudonomas aeroginosa Gram negative bacteria found in soil and dust that can cause serious infections

–Staphylococcus aureus Gram-positive opportunistic human pathogen carried by 30% of the population

Thieves’ killed over 99% of bacteria within 20 minutes

• 20 minutes of diffusing Thieves EO blend– killed 99.2% of all

airborne Micrococcus luteus

– The longer the oil was diffused, the greater the reduction in microorganisms

0

10002000

300040005000

60007000

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Bac

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l Cou

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FU)

0 3 6 9 12

Diffusion Time (minutes)

Evolution from Thieves to Triple O• Thieves – (1996)

– Clove

– Lemon

– Cinnamon Bark

– Eucalytpus radiata

– Rosemary

• Triple O – (2001)

• Clinically tested– Thieves EO Blend

– Oregano

– Thyme

72 Oils grouped by chemistry

Combination of Phenolics and Aldehydes

• Cinnamaldehyde (in cinnamon bark EO) is both an aldehyde and a phenol

• Clove contains antiviral and antibacterial phenolics known as phenylpropanoids

• Thyme and oregano contain simple phenolics (thymol and carvacrol)– Thyme – 55% thymol– Oregano – 65% carvacrol

• Reports show that carvacrol from oregano oil kills spores from Bacillus cereus and Bacillus anthracis (Athrax)– Archives Microbiology, v 174, Oct 2000

• "Oil of Oregano exhibited the greatest antibacterial action against common pathogenic germs such as Staph, E.coli, and Listeria."– Journal of Food Protection, v 64, July 2001

• "Oregano Oil had antibacterial activity against 25 different bacteria."– Journal of Applied Microbiology, v 88, Feb 2000

Penillicin vs Oregano EO

• Research at Weber State University by Sue Chao found that 12 microliters of oregano had the antibacterial equivalence of 10 units of penicillin.

microLiterE COLI Zone of Inhibition (cm)

Oregano EO 3 2.4

Penicillin 10 units no activity

microLiter

STAPH AUREUS Zone of Inhibition

(cm)

Oregano EO 12 3.2

Penicillin 10 units 3.2

Oregano vs. Penicillin

• Harry Preuss, MD, a professor of physiology and biophysics at Georgetown University found that oregano oil (in particular, the chemical component carvacrol) works as well as antibiotics on staphylococcus.

• In a test tube assay, oregano oil combined with staph inhibited its growth as effectively as penicillin and other antibiotics.

Resisting Infections

• In the second phase of this study, 50% of mice receiving oregano for 30 days survived staph infections compared to none in the control group.

• "Natural oils may turn out to be valuable adjuvants or even replacements for many anti-germicidals under a variety of conditions.“– Harry Preuss, MD, professor of physiology

and biophysics, Georgetown University– Science Daily, Oct 11, 2001

Evolution from Triple O to Inner Defense

• Inner Defense – (2007)– Triple O

– Citronella

• Triple O – (2001)– Thieves EO Blend

– Oregano

– Thyme

Citronella• Citronella is high in powerfully

antifungal aldehydes

• Columbia University in New York reported in 2006 that citronella has 8 times the antimicrobial action as lemon oil

INNER DEFENSENature’s ultimate armor