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Gene Pulser Xcell Electroporation System Instruction Manual Catalog Numbers 165-2660, 165-2661, 165-2662, 165-2666, 165-2667, and 165-2668 For technical service call your local Bio-Rad office or in the U.S. call 1-800-4BIORAD (1-800-424-6723) On the Web at discover.bio-rad.com

Gene Pulser Xcell Electroporation System - Bio- · PDF fileThe Gene Pulser Xcell electroporation system is warranted against defects in materials and workmanship for 1 year. ... 3.3

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Text of Gene Pulser Xcell Electroporation System - Bio- · PDF fileThe Gene Pulser Xcell...

  • Gene Pulser Xcell

    Electroporation System

    Instruction Manual

    Catalog Numbers165-2660, 165-2661, 165-2662, 165-2666,165-2667, and 165-2668For technical servicecall your local Bio-Rad office orin the U.S. call 1-800-4BIORAD(1-800-424-6723)On the Web at discover.bio-rad.com

  • Warranty

    The Gene Pulser Xcell electroporation system is warranted against defects in materials and workmanshipfor 1 year. If any defects occur in the instruments or accessories during this warranty period, Bio-RadLaboratories will repair or replace the defective parts at its discretion without charge. The followingdefects, however, are specifically excluded:

    1. Defects caused by improper operation.

    2. Repair or modification done by anyone other than Bio-Rad Laboratories or an authorized agent.

    3. Damage caused by substituting alternative parts.

    4. Use of fittings or spare parts supplied by anyone other than Bio-Rad Laboratories.

    5. Damage caused by accident or misuse.

    6. Damage caused by disaster.

    7. Corrosion caused by improper solvent or sample.

    This warranty does not apply to the following parts:

    Fuses

    For any inquiry or request for repair service, contact Bio-Rad Laboratories. Inform Bio-Rad of the modeland serial number of your instrument.

    IMPORTANT: This Bio-Rad instrument is designed and certified to meet EN61010* and the EMCrequirements of EN61326 (for Class A) safety standards. Certified products are safe to use whenoperated in accordance with the instruction manual. This instrument should not be modified or alteredin any way. Alteration of this instrument will:

    Void the manufacturer's warranty

    Void the EN61010 safety certification

    Create a potential safety hazard

    Bio-Rad Laboratories is not responsible for any injury or damage caused by the use of this instrument forpurposes other than those for which it is intended, or by modifications of the instrument not performedby Bio-Rad Laboratories or an authorized agent.

    *EN61010 is an internationally accepted electrical safety standard for laboratory instruments.

    Model : Gene Pulser Xcell

    Catalog No.: 165-xxxx

    Date of Delivery:

    Serial No.:

    Invoice No.:

    Purchase Order No.:

  • Table of Contents

    Section 1 Introduction and Safety Information: The Gene Pulser XcellSystem ........................................................................................................1

    1.1 General Safety Information ............................................................................1

    1.2 Electrical Hazards ........................................................................................2

    1.3 Mechanical Hazards......................................................................................2

    1.4 Other Safety Precautions ..............................................................................2

    Section 2 Unpacking and System Installation ........................................................3

    2.1 Unpacking the System Components ............................................................3

    2.2 Setting up the System ..................................................................................4

    2.2.1 Setting up the Gene Pulser Xcell Main Unit and Connecting the ShockPod (Cat. #s 165-2660, 165-2661, 165-2662,165-2666) ........................................................................................4

    2.2.2 Connecting the PC Module to the Gene Pulser Xcell Main Unit (Cat. #s 165-2660, 165-2662, and 165-2668) ..........................5

    2.2.3 Connecting the CE Module to the Gene Pulser Xcell Main Unit (Cat. #s 165-2660, 165-2661, and 165-2667) ..........................6

    2.2.4 ShockPod (Cat. #s 165-2660, 165-2661, 165-2662, and165-2669) ........................................................................................6

    Section 3 Gene Pulser Xcell Operating Instructions ..............................................8

    3.1 Section Overview ..........................................................................................8

    3.2 Front Panel and Home Screen ......................................................................9

    3.2.1 Description of Keypad......................................................................9

    3.2.2 Home Screen ................................................................................10

    3.2.3 Help Screens ................................................................................11

    3.3 Manual Operation........................................................................................12

    3.3.1 Manual Operation (Guide Guide) ....................................................12

    3.3.2 Electroporation using Exponential Decay Pulses ............................12

    3.3.3 Electroporation Specifying Time Constant......................................14

    3.3.4 Electroporation using Square Wave Pulses ....................................15

    3.3.5 Results Screens ............................................................................17

    3.3.6 Saving a Program from Manual Operation......................................19

    3.3.6A Saving in a Location without a Named User Entry................20

    3.3.6B Saving in a Location with a Named User Entry ....................20

    3.4 Pre-Set Protocols........................................................................................21

    3.4.1 Using a Pre-set Protocol (Quick Guide) ..........................................21

    3.4.2 Electroporation using a Pre-Set Protocol........................................22

    3.4.3 Modifying Pre-Set Protocol Parameters ........................................25

    3.4.4 Saving Changes to Pre-Set Protocols ............................................25

    3.5 User Protocols ............................................................................................26

  • 3.5.1 Using a User Protocol (Quick Guide) ..............................................26

    3.5.2 Creating a New User Name ..........................................................26

    3.5.3 Creating a New User Protocol........................................................26

    3.5.4 Modifying a User Protocol ..............................................................30

    3.5.5 Deleting a User Name and a User Protocol....................................31

    3.5.6 Renaming a User Name or a User Protocol ..................................33

    3.6 Last Pulse ..................................................................................................34

    3.7 Optimize Operation ....................................................................................34

    3.8 Data Management ......................................................................................36

    3.9 Measurements ............................................................................................39

    3.9.1 Sample Resistance Measurements ................................................39

    3.9.2 Calibration and Measurement of Capacitors in the CE Module..................................................................................................40

    3.10 User Preferences ........................................................................................41

    3.10.1 Setting the Clock ..........................................................................41

    3.10.2 Adjusting the Screen Intensity ........................................................42

    3.10.3 Sleep Function Setting ..................................................................42

    3.11 The Pulse Trac System ..............................................................................43

    3.11.1 Pulse Trac System Description ......................................................43

    3.11.2 Pulse Trac Diagnostic Algorithm ....................................................44

    Section 4 Overview of Electroporation Theory ......................................................44

    4.1 Exponential Decay Pulses ..........................................................................45

    4.2 Square Wave Pulses ..................................................................................45

    Section 5 Factors Affecting Electroporation: Optimizing Electroporation ........................................................................................48

    5.1 Cell Growth ................................................................................................48

    5.2 DNA............................................................................................................49

    5.3 Electroporation Media ................................................................................49

    5.4 Temperature................................................................................................50

    Section 6 Electroporation of Bacterial Cells ..........................................................52

    6.1 Escherichia coli ..........................................................................................52

    6.1.1 Preparation of Electrocompetent Cells............................................52

    6.1.2 Electroporation ..............................................................................53

    6.1.3 Solutions and Reagents ................................................................53

    6.2 Staphylococcus aureus ..............................................................................54

    6.2.1 Preparation of Electrocompetent Cells............................................54

    6.2.2 Electroporation ..................................................