H I S T O L O G YWhat is histology?

How are histologic sections made?

MICROSCOPIC EXAMINATION

Histology is the study of the microscopic anatomy of cells and tissues.

STEP 1:

Each body organ and tissue type has a characteristic microscopic appearance. Review the photos shown here and then the glass slides. Try to identify what tissue type is shown on each slide.

Tissue sections on glass slides are stained and then examined via microscope.

H&E (Hematoxylin & Eosin) is the most commonly used stain in medical diagnosis.

Eosin

Hem

atox

ylin

STEP 2:Fixation & Processing

STEP 3:Tissue embedding in paraffin

STEP 4:Microscopic evaluation

STEP 5:Slide staining

STEP 6:Block cut to make slides

Cutting the tissue

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