Hull et. al. 2015. 43

SUPPLEMENTAL FIGURE LEGENDS: Supplemental Figure 1: Western blots used to generate the densitometry values depicted in Figure 3.

Supplemental Figure 2: Western blots used to generate the densitometry values depicted in figure 6. Supplemental Figure 3: Densitometry measurements for the blots depicted in Figure 7. Bars depict the mean ± SEM of measurements normalized to GAPDH. Data are expressed in arbitrary units. P values < 0.05 using ANOVA and the Newman-Keuls post-test were considered significant. n = 3-4/group. Supplemental Figure 4: Transmission electron micrographs of left ventricle (LV) from wild-type and cardiac-specific overexpression of HO-1 (cs-HO-1) mice treat with vehicle or 14 or 60 days after treatment with doxorubicin. Micrographs were captured at 1650x total magnification. Scale bar represents 2 µm. n = 3 – 5 mice per group, with at least 5 images evaluated per mouse. Images were assessed by a reviewer blinded to the different groups.

B-Actin

COX3

ND1

NRF1

PGC1α

TFAM

Polγ

cs-HO-1cs-HO-1WT WT

Vehicle DOX

SUPP FIGURE 1

B-Actin

Fis1

Mfn1

Mfn2

DRP1

GAPDH

WT cs-HO-1 WT cs-HO-1Vehicle DOX

B

cs-HO-1cs-HO-1WT WT

Vehicle DOX

A

SUPP FIGURE 2

SUPP FIGURE 3

SUPP FIGURE 4

Wild-Type cs-HO-1Ve

hicl

eD

ay 1

4D

ay 6

0

A B

D

E

C

F

Cropped Figure 7

cs-HO-1cs-HO-1

HO-1

PINK1

GAPDH

32 kDa

63 kDa

37 kDa

~50 kDa

WTcs

HO-1 WT WT

Vehicle DOX Day 5 DOX Day 14

Pon S

B

Figure 7 Original Blot – no lanes croppedLanes:cc12cc34567891011121314

Lanes:1234567891011121314

HO-1

PINK1

GAPDH

Pon S

Full uncropped gel for Figure 7

c = lanes cropped from the image of the blot in figure 7.Numbered lanes were incorporated into figure 7.The 4th, and 5th cropped lanes were superfluous, depicting resultsfrom cs-HO-1 mice already shown in lane 2. The 2nd croppedlane was the incorrect genotype. The 1st cropped lane wassuperfluous, showing the same control as lane 1.