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IS THIS COOL?

IS THIS COOL?. Well that’s what we are going to do

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Page 1: IS THIS COOL?. Well that’s what we are going to do

IS THIS COOL?

Page 2: IS THIS COOL?. Well that’s what we are going to do

IS THIS COOL?

Page 3: IS THIS COOL?. Well that’s what we are going to do

IS THIS COOL?

Page 4: IS THIS COOL?. Well that’s what we are going to do

Well that’s what we are going to do

Page 5: IS THIS COOL?. Well that’s what we are going to do

Transgenesis

Page 6: IS THIS COOL?. Well that’s what we are going to do

Transgenesis

• Multiple goals:

Page 7: IS THIS COOL?. Well that’s what we are going to do

Transgenesis

• Multiple goals:

Page 8: IS THIS COOL?. Well that’s what we are going to do

Transgenesis

• Multiple goals:

Page 9: IS THIS COOL?. Well that’s what we are going to do

Transgenesis

• Multiple goals:

Page 10: IS THIS COOL?. Well that’s what we are going to do

How does this happen?

Page 11: IS THIS COOL?. Well that’s what we are going to do
Page 12: IS THIS COOL?. Well that’s what we are going to do

Could we make insulin?

Page 13: IS THIS COOL?. Well that’s what we are going to do

GFP (green fluorescent protein)

• Isolated from a jellyfish with this trait.

Page 14: IS THIS COOL?. Well that’s what we are going to do

Why modify organisms with GFP?

Page 15: IS THIS COOL?. Well that’s what we are going to do
Page 16: IS THIS COOL?. Well that’s what we are going to do
Page 17: IS THIS COOL?. Well that’s what we are going to do
Page 18: IS THIS COOL?. Well that’s what we are going to do
Page 19: IS THIS COOL?. Well that’s what we are going to do
Page 20: IS THIS COOL?. Well that’s what we are going to do

Quick review quiz

Page 21: IS THIS COOL?. Well that’s what we are going to do

Quick review quiz

1. What does LB have in it?

Page 22: IS THIS COOL?. Well that’s what we are going to do

Quick review quiz

1. What does LB have in it?

2. What does lysogenic mean?

Page 23: IS THIS COOL?. Well that’s what we are going to do

Quick review quiz

1. What does LB stand for?

2. What does lysogenic mean?

3. What does lyophilized mean?

Page 24: IS THIS COOL?. Well that’s what we are going to do

Quick review quiz

1. What does LB have in it?

2. What does lysogenic mean?

3. What does lyophilized mean?

4. What is ampicillin?

Page 25: IS THIS COOL?. Well that’s what we are going to do

Quick review quiz

1. What does LB have in it?

2. What does lysogenic mean?

3. What does lyophilized mean?

4. What is ampicillin?

5. What is arabinose?

Page 26: IS THIS COOL?. Well that’s what we are going to do

Quick review quiz

1. What does LB have in it?

2. What does lysogenic mean?

3. What does lyophilized mean?

4. What is ampicillin?

5. What is arabinose?

6. What is the purpose of the LB/amp/ara plate?

Page 27: IS THIS COOL?. Well that’s what we are going to do

Quick review quiz

1. What does LB have in it?

2. What does lysogenic mean?

3. What does lyophilized mean?

4. What is ampicillin?

5. What is arabinose do?

6. What is the purpose of the LB/amp/ara plate?

7. What is the purpose of the LB/amp plate?