CYTOLOGY Lab.

Cytology 1

1. Single mitochondria, RER & Lipid droplet.2. Mitochondria ,RER & Secondery

lysosomes.3. Mitochondria, SER & glycogen granules.4. T.S of centriole , Golgi complex &coated

vesicle.5. Golgi complex.6. Peroxisome & Golgi complex.7. Golgi apparatus in epididymis

Cytology 2

8. Nucleus & RER (EM).9. Nuclear pore & rER continuous with the

outer nuclear membrane(EM).10. Melanin pigment in negroid skin11. Glycogen granules in liver. Slide12. lipid droplet.(EM)

Single mitochondria, rER

& lipid droplet

Mitochondria .Outer membrane and inner folded membrane.

Cristae Rough endoplasmic reticulum :

Consists of stacks of flattened cisternae.Membranous organells .

Polyribosomes on cytosolic surface of the membrane

Mitochondria, rER & secondary lysosomes

Secondary lysosomes. Membranous

cell organelles..Appears heterogenous due to presence of digested materials

sER , Mitochondria &

glycogen granules

Smooth endoplasmic reticulum. Absence of ribosomes (smooth)Cisternae is tubular in shape (notflattened(

Mostly found in cells synthesizing steroid hormones, cholesterol and triglycerides like liver and endocrine cells.

Glycogen granules: appears as collections

of electron-dense particles (rosett appearance)

T.S. of centriole, Golgi

complex & coated vesicle

Centrioles are non-membranous organelles.

centriole is a hollow cylinder with a dense wall around an electron lucent central core.

Its wall is made up of 9 bundles of microtubules, each bundle consists of 3 microtubules (triplets).

Golgi complex

Cis face and trans face, transfer vesicles secretory vesicles.

Cis-face (immature or forming face) which is usually convex. It is directed towards rough endoplasmic reticulum and its cisternae are highly fenestrated.

Trans-face (mature or secretory face) is usually concave and is directed towards the plasmalemma. The lumen of its cisternae tends to be wider.

6- Nucleus & rERNUCLEUS: The nuclear envelope, chromatin, nucleolus, and nuclear matrix.

Peroxisome & Golgi complexSpherical membranous organelles.Have a dense central region called crystaline core.Fine granular homogenous content.

Lipid droplets & cell membrane

A cell inclusion, Electron dense and homogenous, Variable in size.

Nuclear pores & rER continous with the outer

nuclear membrane

Golgi apparatus in epididymis

Dark stained area in cells of epididymis --- Supranuclear in position

Glycogen granules in liverA cytoplasmic inclusion, present in large amounts in liver cells (hepatocytes). Glycogen appears as dark acidophilic granules

Melanin pigments in negroid skin

A cytoplasmic deposit - Dark brown pigment. - In the epidermis of skin.