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Lab safety sheet for proteins lab
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Page 1 of 5
LS23L SAFETY SHEET FOR PROTEINS & SDS‐PAGE LAB
You must review this sheet and the attached SOPs carefully before coming to lab. You are responsible for following all safety guidelines while in lab and you will be tested on this material in the pre‐lab quiz.
STANDARD OPERATING PROCEDURES (SOPS) REFERRED TO IN THIS LAB:
TGS Buffer (1x concentration)
iGels (Tris Glycine gels manufactured by NuSep)
POTENTIAL HAZARDS:
Both gels and buffer contain material which can cause respiratory tract, eye and skin irritation. Contains material
which causes damage to the following organs: skin, eyes. May be harmful if absorbed through skin or if swallowed.
May be harmful if inhaled.
ENGINEERING AND WORK PRACTICE CONTROLS:
Chemicals should only be used in lab rooms (YHS 2336, 2370, 2379)
PERSONAL PROTECTIVE EQUIPMENT (PPE):
In addition to standard lab attire, you must wear:
Safety glasses or goggles when working with your protein sample and loading the gel
Gloves when handling your protein sample and loading the gel
Lab coat for the entire time you are in the lab room
WASTE DISPOSAL:
Used pipette tips and microcentrifuge tubes – regular trash
Microcentrifuge tubes containing small volume of protein mix – regular trash
Your TA will handle the disposal of the gels and the buffer
Page 2 of 5
Standard Operating Procedure for work with
Chemical name: iGels by NuSep CAS #: See list at end of SOP
PI: Dr. Gaston Pfluegl Updated: 3/28/2013
Building: Young Hall South Room(s): YHS 2344, 2336, 2370, 2379
1. Circumstances of Use/Storage:
iGels are pre‐cast polyacrylamide gels manufactured by NuSep and stored at 4C in YHS 2344. The gels are carried
into the lab rooms (YHS 2336, 2370, 2379) for the separation of proteins in the proteins lab in LS23L. Gels are
enclosed in a plastic cassette. Manufacturer’s description “Clear, solid, gel encased in a hard plastic cassette,
individually packaged in a plastic sachet in storage buffer (pH 8.5).”
2. Potential Hazards:
Inhalation: The gel is unlikely to pose an inhalation hazard because it is encased in a plastic cassette. Skin contact: Data suggests that this product should present no significant problems. Eye contact: This product may be mildly irritating to eyes. However, it is unlikely to cause any more than mild transient discomfort. It is also unlikely to cause any lasting effects. Ingestion: Ingestion of the cassette is unlikely. Gel may cause gastrointestinal irritation if swallowed. Chronic Exposure: No specific data is available for the product for chronic exposure symptoms. CARCINOGEN STATUS: Ingredients are not classified as human carcinogens by the International Agency for Research on Cancer (IARC).
3. Engineering Controls:
N/A
4. Work Practice Controls:
iGels will only be used in the lab rooms (YHS 2336, 2370, 2379).
5. Personal protective equipment (PPE):
All personnel are required to wear the following personal protective equipment whenever handling this material (in
addition to standard lab attire): Safety glasses or goggles, gloves, lab coat.
6. Transportation and Storage:
iGels are stored in the prep room (YHS 2344) at 4C. Individually packaged gels are sent out to the lab rooms (YHS
2336, 2370, 2379) for use.
7. Waste Disposal:
LS Core lab staff is responsible for the safe collection, preparation and proper disposal of waste. iGels are not
considered toxic and can be disposed of as non‐hazardous waste.
8. Exposures/Unintended contact:
UCLA Campus Police – 911 (tell dispatcher you are at UCLA if calling from cell) or 310.825.1491 Environmental Health and Safety ‐ x59797 (310.825.9797) Emergency Room (Ronald Reagan Medical Center) – x52111 (310.825.2111)
Page 3 of 5
Complete the incident report found on the LS Core Labs prep server and notify EH&S of any serious accidents within
8 hours (310.825.9797).
Skin Contact
Data suggests that this product should present no significant problems.
Eye Contact
This product may be mildly irritating to eyes. However, it is unlikely to cause any more than mild transient
discomfort. It is also unlikely to cause any lasting effects. Quickly and gently blot or brush away chemical.
Immediately flush the contaminated eye(s) with lukewarm, gently flowing water for 5 minutes, or until the chemical
is removed while holding eyelids open. If irritation persists, obtain medical advice.
Inhalation
The gel is unlikely to pose an inhalation hazard because it is encased in a plastic cassette.
Ingestion
Ingestion of the cassette is unlikely. Gel may cause gastrointestinal irritation if swallowed. Drink plenty of water and
seek immediate medical attention.
NO DATA ON CHRONIC EXPOSURE. INGREDIENTS NOT CLASSIFIED AS HUMAN CARCINOGENS.
9. Spill Procedure: All large spills must be reported to EH&S (310.825.9797) as soon as possible.
N/A
10. Authorized Personnel: LS Core Lab Staff
LS Core TAs and UAs (only after TA/UA training meeting)
LS Core Students (only under supervision of trained TAs)
11. Required Training: LS Core Lab Staff training should include the following:
Review of the current MSDS
Review of the departmental safety procedures
Review of the UCLA Chemical Hygiene Plan
Completion of the UCLA Laboratory Safety class administered by EH&S
Completion of annual online refresher Laboratory Safety class administered by EH&S
Completion of annual PPE training
12. Composition/Information on Ingredients:
Ingredients CAS No. Proportion, % Polyacrylamide 9003‐05‐8 < 30 * TRIS 77‐86‐1 < 2.5 Sodium azide * 26628‐22‐8 < 0.004 ** Water 7732‐18‐5 to 100
* Average concentration in most highly concentrated gel, other gels contain less. ** Sodium azide in storage buffer only.
Page 4 of 5
Standard Operating Procedure for work with
Chemical name: TGS buffer (1x solution) CAS #: See list at end of SOP
PI: Dr. Gaston Pfluegl Updated: 3/28/2013
Building: Young Hall South Room(s): YHS 2344, 2336, 2370, 2379
13. Circumstances of Use/Storage:
TGS buffer 10x concentrate will be purchased in 4L cubes and stored in the prep room (YHS 2344). Prep staff will
dilute to 1x in a 10L carboy. Bottles of 1x TGS (two 50 mL bottles per section) will be carried out to the lab rooms
(YHS 2336, 2370, 2379) as part of the proteins SDS‐PAGE lab in LS23L. Used buffer will be disposed of as described
below.
14. Potential Hazards: WARNING! Causes respiratory tract, eye and skin irritation. Contains material which causes damage to the following
organs: skin, eyes. May be harmful if absorbed through skin or if swallowed. May be harmful if inhaled.
15. Engineering Controls: N/A
16. Work Practice Controls:
TGS buffer will only be used in the lab rooms (YHS 2336, 2370, 2379) at the diluted 1x concentration.
17. Personal protective equipment (PPE):
All personnel are required to wear the following personal protective equipment whenever handling this material (in
addition to standard lab attire): Safety glasses or goggles, gloves, lab coat.
18. Transportation and Storage: TGS 10x concentrate is stored in the prep room (YHS 2344). 1x bottles are carried to lab rooms (YHS 2336, 2370,
2379). Containers should be kept tightly capped.
19. Waste Disposal:
Per EH&S, the 1.0x TGS buffer used in the LS Core Labs, uncontaminated and in a pH range of 6.0 – 8.0 is approved
for drain disposal. Used buffer should be poured down the sink and flushed with water.
This is an exception for our labs only and it should not be assumed that other buffers can be drain disposed.
20. Exposures/Unintended contact:
UCLA Campus Police – 911 (tell dispatcher you are at UCLA if calling from cell) or 310.825.1491 Environmental Health and Safety ‐ x59797 (310.825.9797) Emergency Room (Ronald Reagan Medical Center) – x52111 (310.825.2111)
Complete the incident report found on the LS Core Labs prep server and notify EH&S of any serious accidents within
8 hours (310.825.9797).
Skin Contact
Page 5 of 5
Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and
shoes. Get immediate medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get
medical attention immediately.
Eye Contact
Immediately flush eyes with plenty of water for at least 15 minutes, lifting upper and lower eyelids occasionally.
Check for and remove contact lenses. Get medical attention immediately.
Inhalation
Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical
attention.
Ingestion
If swallowed, DO NOT INDUCE VOMITING. Give small quantities of water to avoid inducing vomiting. If vomiting
occurs, the head should be kept low so the vomit does not enter the lungs. Never give anything by mouth to an
unconscious person. Get medical attention immediately.
21. Spill Procedure: All large spills must be reported to EH&S (310.825.9797) as soon as possible.
Use personal protective equipment as described above. Cover and recover liquid when possible. Collect liquid in an
appropriate container or absorb with an inert material (e.g. vermiculite, dry sand, earth) from the spill kit available
in each room and place in a chemical waste container for disposal.
22. Authorized Personnel: LS Core Lab Staff
LS Core TAs and UAs (only after TA/UA training meeting)
LS Core Students (only under supervision of trained TAs)
23. Required Training: LS Core Lab Staff training should include the following:
Review of the current MSDS
Review of the departmental safety procedures
Review of the UCLA Chemical Hygiene Plan
Completion of the UCLA Laboratory Safety class administered by EH&S
Completion of annual online refresher Laboratory Safety class administered by EH&S
Completion of annual PPE training
24. Composition/Information on Ingredients:
Name CAS number % by weight (at 1x dilution) Water 7732‐18‐5 98.2 – 98.1 Glycine 56‐40‐6 1.4 – 1.5 Tris (hydroxymethyl)aminomethane 77‐86‐1 0.3 Sodium Dodecyl Sulfate 151‐21‐3 0.1