Lab essentials and accessories - Saixx Technolog Qiagen/Catalogo2007/PG2007... 384 QIAGEN Product Guide

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  • 380 www.qiagen.com QIAGEN Product Guide 2007

    10 Lab essentials and accessories

    CH_10_4C_QIAGEN_PG_2007.qxd 10.11.2006 12:53 Uhr Seite 380

  • Other products and services

    QIAGEN Product Guide 2007 Sample & Assay Technologies 381

    www.qiagen.com/PG/accessories 10.1 Molecular biology buffers, reagents, and enzymes

    Buffers and reagents Molecular biology buffers and reagents 382

    Enzymes for sample preparation Enzymes 383 DNA markers for electrophoresis GelPilot DNA Molecular

    Weight Markers 384 DNase digestion for RNA purification RNase-Free DNase Set 387

    10.2 Extraction of nucleic acids from organic solvents Extraction of nucleic acid from organic solvents MaXtract Low and High Density 388

    10.3 Sample disruption and homogenization Rapid homogenization of cell and tissue lysates, spin-column format QIAshredder Homogenizer 390

    Low-throughput sample disruption using disposable probes TissueRuptor System 391

    High-throughput sample disruption TissueLyser and Accessories 392

    10.2 Vacuum manifolds Vacuum-driven processing of 96-well plates, 8-well strips, and spin columns QIAvac Vacuum Manifolds and Accessories 394

    10.5 Centrifuges Universal laboratory centrifuge Centrifuge 4-15C 396

    Universal laboratory centrifuge, refrigerated Centrifuge 4K15C 396

    10.6 Magnets For separating magnetic particles in 96-well plates, tubes, or culture flasks Magnets 397

    10.7 Tape sheets and plasticware 398

    New

    New

    New

    New

    Lab essentials and accessories 10

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  • 382 www.qiagen.com QIAGEN Product Guide 2007

    Lab essentials and accessories 10.1

    Molecular Biology Buffers and ReagentsNew

    Product Contents Cat. no.

    QIAGEN® RNase 250 µl (30 U/µl) RNase Inhibitor 129918 Inhibitor (7500 U) in 2 mM KH2PO4, 8 mM

    Na2HPO4, 3 mM KCl, 150 mM NaCl, pH 7.4, and 50% glycerol

    QIAGEN RNase 500 µl (30 U/µl) RNase Inhibitor 129919 Inhibitor (15,000 U) in 2 mM KH2PO4, 8 mM

    Na2HPO4, 3 mM KCl, 150 mM NaCl, pH 7.4, and 50% glycerol

    Nuclease-Free 10 x 50 ml nuclease-free water 129114 Water (10 x 50 ml) prepared without the use of DEPC

    (diethylpyrocarbonate)

    Nuclease-Free 1000 ml nuclease-free water 129115 Water (1000 ml) prepared without the use of DEPC

    (diethylpyrocarbonate)

    Nuclease-Free 5 liters nuclease-free water 129117 Water (5 liters) prepared without the use of DEPC

    (diethylpyrocarbonate)

    Buffer RLT (220 ml) 220 ml lysis buffer for RNeasy Kits 79216

    Buffer TBE, 5x tris-borate–EDTA (0.45 M 129217 5x (5 liters) tris-borate, 0.01 M EDTA, pH 8.3).

    Nuclease-free

    Buffer TAE, 50x tris-acetate–EDTA 129237 50x (5 liters) (2 M tris-acetate,

    0.05 M EDTA, pH 8.3). Nuclease-free

    IPTG (5 g) Isopropyl-thio-2-D-galactopyranoside. 129921 White powder. Molecular weight 238.3. Dioxane-free

    X-Gal (1 g) 5-bromo-4-chloro-3-indolyl-β-D- 129931 galactopyranoside. White crystalline powder. Molecular weight 408.6

    GelPilot DNA See page 384 for more information Molecular Weight Markers

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  • QIAGEN Product Guide 2007 Sample & Assay Technologies 383

    Lab essentials and accessories 10.1

    Enzymes

    For further information: www.qiagen.com/PG/accessories

    Product Contents Cat. no.

    GelPilot DNA 5x gel loading dye containing 239901 Loading Dye, 5x bromophenol blue, xylene cyanol,

    and orange G

    dNTP Set, 100 mM each dATP, dCTP, dGTP, 201912 PCR Grade, 4 x 100 µl dTTP for 1000 x 50 µl PCR

    reactions

    dNTP Set, 100 mM each dATP, dCTP, dGTP, 201913 PCR Grade, 4 x 250 µl dTTP for 2500 x 50 µl PCR

    reactions

    dNTP Mix, Mix containing 10 mM 201900 PCR Grade (200 µl) each of dATP, dCTP, dGTP, and

    dTTP (1 x 200 µl)

    dNTP Mix, Mix containing 10 mM 201901 PCR Grade (800 µl) each of dATP, dCTP,

    dGTP, and dTTP (4 x 200 µl)

    Product Contents Cat. no.

    RNase-Free DNase Set See page 387 for more information 79254

    QIAGEN Protease (7.5 AU) 7.5 Anson units per vial (lyophilized) 19155

    QIAGEN Protease (30 AU) 4 x 7.5 Anson units per vial 19157 (lyophilized)

    QIAGEN Proteinase K (2 ml) 2 ml (>600 mAU/ml, solution) 19131

    QIAGEN Proteinase K (10 ml) 10 ml (>600 mAU/ml, solution) 19133

    RNase A (17,500 U) 2.5 ml (100 mg/ml; 7000 units/ml, 19101 solution)

    CH_10_4C_QIAGEN_PG_2007.qxd 10.11.2006 12:53 Uhr Seite 383

  • 384 www.qiagen.com QIAGEN Product Guide 2007

    Lab essentials and accessories 10.1

    GelPilot DNA Molecular Weight Markers For easy and accurate sizing of DNA fragments

    ■ Convenient design — intensive DNA fragments and easy-to-remember pattern for fast and accurate DNA sizing

    ■ Ready-to-load buffer — containing 3 tracking dyes to facilitate optimization of agarose gel run time

    ■ Defined band intensities — standard (50 ng) and intensive (100 ng) DNA fragments to aid DNA quantification

    Product description

    The 12 ready-to-use GelPilot markers cover a wide range of fragment sizes guaranteeing an ideal format for every requirement. The DNA fragments in each marker are designed to be easy to remember for fast and accurate DNA sizing. In addition, each marker contains a single intensive band, facilitating DNA quantification and making it simple to determine the DNA size. The unique ready- to-load buffer contains 3 tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time.

    Applications

    GelPilot DNA Molecular Weight Markers are suitable for use when analyzing DNA on an agarose gel.

    New

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  • QIAGEN Product Guide 2007 Sample & Assay Technologies 385

    Lab essentials and accessories 10.1

    GelPilot 50 bp Ladder (100) (cat. no. 239025)

    GelPilot 100 bp Ladder (100) (cat. no. 239035)

    GelPilot 100 bp Plus Ladder (100) (cat. no. 239045)

    GelPilot 200 bp Ladder (100) (cat. no. 239055)

    GelPilot 500 bp Ladder (100) (cat. no. 239075)

    GelPilot 1 kb Ladder (100) (cat. no. 239085)

    GelPilot 1 kb Plus Ladder (100) (cat. no. 239095)

    GelPilot Low Range Ladder (100) (cat. no. 239115)

    GelPilot Wide Range Ladder (100) (cat. no. 239125)

    GelPilot Mid Range Ladder (100) (cat. no. 239135)

    GelPilot High Range Ladder (100) (cat. no. 239145)

    GelPilot Lambda HindIII Marker (100) (cat. no. 239185)

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    bp

    –500 –400 –350 –300 –250 –200 –150

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    bp –15,000 –10,000 –7500 –5000

    –2500

    –1000

    –250

    bp

    –23,130 –9416 –6557 –4361

    –2322 –2027

    –564

    bp –10,000 –8000 –7000 –6000 –5000 –4000

    –3000

    –2000

    –1000

    bp

    –10,000 –8000 –5000

    –2000 –1600

    –1000 –700 –500 –400 –300 –200 –100

    bp

    –1200 –900 –700

    –500

    –300

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    bp

    –600 –500 –400 –300

    –200

    –100

    bp –1500

    –1000 –900 –800 –700 –600 –500 –400

    –300

    –200

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    bp

    –4000 –2200 –2000 –1800 –1600 –1400 –1200 –1000

    –800

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    Clearly Arranged Markers

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  • 386 www.qiagen.com QIAGEN Product Guide 2007

    Lab essentials and accessories 10.1

    Product Contents Cat. no.

    GelPilot 50 bp Ready-to-use DNA marker; 600 µl 239025 Ladder (100) (100 lanes), 9 fragments: 50–500 bp

    GelPilot 100 bp Ready-to-use DNA marker; 600 µl 239035 Ladder (100) (100 lanes), 6 fragments: 100–600 bp

    GelPilot 100 bp Plus Ready-to-use DNA marker; 600 µl 239045 Ladder (100) (100 lanes), 11 fragments: 100–1500 bp

    GelPilot 200 bp Ready-to-use DNA marker; 600 µl 239055 Ladder (100) (100 lanes), 12 fragment: 200–4000 bp

    GelPilot 500 bp Ready-to-use DNA marker; 239075 Ladder (100) 600 µl (100 lanes), 8 fragments:

    500–4000 bp

    GelPilot 1 kb Ready-to-use DNA marker; 600 µl 239085 Ladder (100) (100 lanes), 9 fragments:

    1000–10,000 bp

    GelPilot 1 kb Plus Ready-to-use DNA marker; 600 µl 239095 Ladder (100) (100 lanes), 12 fragments:

    100–10,000 bp

    GelPilot Low Range Ready-to-use DNA marker; 600 µl 239115 Ladder (100) (100 lanes), 6