Laboratory Methods in Laboratory Methods in BiotechnologyBiotechnology

Theory and practice of common biological Theory and practice of common biological laboratory methods: solution preparation, laboratory methods: solution preparation, spectrophotometry, electrophoresis, DNA spectrophotometry, electrophoresis, DNA

manipulations, and biological, chemical, and manipulations, and biological, chemical, and radiological safety radiological safety

Idea Behind the CourseIdea Behind the Course

Provide practical experiences in laboratory Provide practical experiences in laboratory activities that will help students make the activities that will help students make the transition from content-based lower division transition from content-based lower division courses to application orientated senior courses to application orientated senior level courses.level courses.

Provide skills and dispositions needed to Provide skills and dispositions needed to carry out laboratory research as part of a carry out laboratory research as part of a career in the life sciences. This includes career in the life sciences. This includes undergraduate research, working as a undergraduate research, working as a technician, graduate research, and affiliated technician, graduate research, and affiliated professions.professions.

Role in Your Curriculum Role in Your Curriculum

Electives: Molecular Techniques, Cell and Molec, Immunology, Path Micro,Microbial Phys, etc

MCMB Specialty: Lab Methods, Micro, Molecular Genetics

Core: Reasoning, Investigation, Cell Biology, Genetics,

ObjectivesObjectives

To give students To give students experience in experience in dimensional analysis dimensional analysis used in microbiology used in microbiology and cellular and and cellular and molecular biology, molecular biology, and biotechnology and biotechnology

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ObjectivesObjectives

To familiarize the To familiarize the students with the theory students with the theory and practice of and practice of preparing solutions preparing solutions from various starting from various starting materials.materials.

ObjectivesObjectives

To introduce the students to means of preparing To introduce the students to means of preparing sterile media and solutions. sterile media and solutions.

ObjectivesObjectives

To allow students to To allow students to practice quantifying practice quantifying bacteria and viruses, bacteria and viruses, and to measure the and to measure the growth rates of bacterial growth rates of bacterial populations. populations.

ObjectivesObjectives

To allow students to To allow students to quantify proteins, quantify proteins, nucleic acids and other nucleic acids and other chemicals in solution.chemicals in solution.

ObjectivesObjectives

To introduce students to commonly used devices To introduce students to commonly used devices including, but not limited to: microliter pipetters, including, but not limited to: microliter pipetters, centrifuges, spectrophotometers, pH meters, etc. centrifuges, spectrophotometers, pH meters, etc.

ObjectivesObjectives

To introduce the students to the use of enzymes for To introduce the students to the use of enzymes for end-point determination and to the measurement of end-point determination and to the measurement of enzyme activity. enzyme activity.

ObjectivesObjectives

To give students To give students experience in the experience in the techniques for the techniques for the manipulation and manipulation and electrophoretic electrophoretic separation of proteins separation of proteins and DNA.and DNA.

ObjectivesObjectives

To provide information regarding biological, To provide information regarding biological, chemical, radiation, and other aspects of lab safety. chemical, radiation, and other aspects of lab safety.

TextbooksTextbooks

Make sure you bring Make sure you bring this each time.this each time. Seidman and Moore – Seidman and Moore –

our main textour main text Leboffe and Pierce – Leboffe and Pierce –

illustration of techniquesillustration of techniques Also need:Also need:

Calculator!Calculator! Lab NotebookLab Notebook

Additional ResourcesAdditional Resources

Brock – background information on theoryBrock – background information on theory Day – advice on writing (humor)Day – advice on writing (humor) Robyt - Source of many handouts/additional readingsRobyt - Source of many handouts/additional readings CBE Manual – Style guide for preparation of manuscripts CBE Manual – Style guide for preparation of manuscripts Don’t buy theseDon’t buy these

WebpageWebpagehttp://cstl-csm.semo.edu/champine/BI245/index.html

Lab Activities Lab Activities Lecture Topics Lecture Topics Homework Assignments Homework Assignments In Class Assignments In Class Assignments Manuscripts Manuscripts Notebook Guidelines Notebook Guidelines Syllabus Syllabus Grade BookGrade Book