Lampiran Fix Print

8/19/2019 Lampiran Fix Print

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LAMPIRAN - LAMPIRAN


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Lampiran I. Surat Keterangan Serbuk Daun Jarak Pagar ( Jatropha curcas Linn).


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Lampiran II. Surat Persetujuan Etik ( Ethical Approval ) untuk peneitian jarak pagar


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Lampiran III. Data Per!itungan Ren"eman #er"asarkan Ekstak Kenta Etan$ Daun

Jarak Pagar

- Ekstrak Etanol

Bobot ekstrak etanol = 53,3 gram

Rendemen =53,3

1000 x 100 %

= 5,33 %

- Fraksi tidak larut n-heksan

Bobot fraksi = ekstrak kental etanol fraksi tidak larut n-heksan

= 53,3 gram !,"! gram

= #5,#3 gram

Rendemen =45,43

53,3 x 100 %

= "5,$3 %

- Fraksi larut loroform

Bobot fraksi = 3,35 gram

Rendemen =3,35

53,3 x 100 %

= &,$' %

-


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Lampiran I%. Data !asi uji antiin&amasi &raksi k$r$&r$m "aun J.curcas pa"a kaki

tikus 'istar ang "iin"uksi karagenan *

(abel )* +ata olume edema setia 1 .am selama & .am engamatan

/aktu

.am

elomok ) ml elomok )) ml

1 $ 3 # 1 $ 3 #

2o

1

0,'$

5 0," 0,"1 0,&5

1 1

1 1,5 1,1 1,15 1,15 1,$5 1,15 1,15 1,05

$

1,5

1,3$

5 1,1!5 1,$!5 1,$5 0,'5 1,$ 1,$

3 1,! 1,# 1,$ 1,$5 1,,$5 0,"5 1,1!5 1,15

# 1," 1,! 1,$$5 1,3$5 1,$ 0,"5 1,1 1,05

51,5

1,5$5 1,$ 1,05 1,15 0,!5 1,0!5 1,05

& 1,$5 1,$ 1,1 1,05 1 0," 1 1

/aktu

.am

elomok ))) ml elomok )2 ml

1 $ 3 # 1 $ 3 #

2o

1

1,0$

5 1 1,$ 1 1,1 0,9 1

1

1,1!5

1,1!

5 1,15 1,3 1,3!5 1,3 1,375 1,3

$ 1,$$5 1,$ 1,$5 1,55 1,55 1,45 1,4 1,53

1,3

1,$$

5 1,3 1,&5 1,&5 1,45 1,35 1,5

#

1,3

1,$!

5 1,3 1,35 1,5!5 1,55 1,45

1,37

5

5

1,1

1,#!

5 1,1 1,$5 1,#$5 1,6 1,4

1,37

5

&1,1 1,35 1,05 1,$5 1,$!5 1,5 1,3

1,32

5

eterangan

elomok 1 = kontrol negatif

elomok $ = kontrol ositif

elomok 3 = F+4 150 mg

elomok # = F+4 300 mg

elomok 5 = F+4 &00 mg

/aktu

.am

elomok 2 ml

1 $ 3 #

2o 0,'!5 1,05 1,05 1,1!5

1 1,$ 1,$ 1,$ 1,35

$ 1,$5 1,$$5 1,3$5 1,#5

3 1,35 1,3!5 1,#$5 1,&

# 1,3 1,#5 1,$!5 1,!5

5 1,$5 1,35 1,1!5 1,5!5

& 1,1 1,$$5 1,0!5 1,#5


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(abel $* Rata-rata resentase kenaikan olum edema ada kelomok kontrol dan

kelomok erlakuan fraksi kloroform daun J.curcas setia 1 .am selama & .am

engamatan

Rata-rata Kenaikan %$ume E"em (*) + SE,aktu

(Jam ke-)

Ke$mp$k

I II III I% %

1 3',10 6 &,"' 30,#" 6 1& 13,"! 6 1,'5 3#,&$ 6 !,$# 1&,&3 6 $,15

$ #',"! 6 3,#5 $!,!' 6 &,$3 $3,#3 6 $,53 #",0' 6 5,5! $3,&$ 6 $,51

3 5&,'0 6 #,5& $$,0! 6 3,5' $',$5 6 3,&' #',$0 6 &,!' 35,3$ 6 1,5"

# !0,&3 6 !,0$ 1&,##6 5,! $#,$$ 6 #,1$ #',$5 6 5,"' 35,#5 6 5,&'

5 #',03 6 &,"" 10,!$ 6 $,&3 1!,0$ 6 ',0& #5,$5 6 3,"0 $5,&" 6 #,!"

& 30,"! 6 $,5" 5,!! 6 5,!& 1$,!$ 6 &,#& 35,$0 6 3,5! 13,"1 6 #,3'

(abel 3* +ata 7rea 8nder 9ure: 7890-& % .am ersentase kenaikan olum edematerhada ;aktu setia 1 .am selama & .am engamatan

/aktu

.am

elomok ) ml elomok )) ml

1 $ 3 # 1 $ 3 #

2o 0 0 0 0 0 0 0 0

1 $5 ',#& $1,"! $1,"! 1$,5 3",#& !,5 $,5

$ 50 31,0" #5,31 51,5& $5 &1,5# 1!,5 1$,5

3 &0 #!,$' #",#3 5!,"1 $5 3",#& 1",!5 1!,5

# !5 &!,5! 51,5& &0,'3 $$,5 30,!! 13,!5 105 &5 !#,3$ 51,5& #",#3 1!,5 $3,0" ",!5 5

& 3!,5 #!,$' #3,!5 31,$5 !,5 1',$3 3,!5 $5

kumulati

f 31$,5

$!!,0

$$&$,5

$!1,"!

5 110 $11,5# !0 50

urata 6


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Lampiran %. 8asi anaisis statistik A9: "ari !ubungan persentase kenaikan ;$um

e"ema ter!a"ap 'aktu pa"a uji e&ek antiin&amasi &raksi k$r$&$rm

"aun J. curcas pa"a kaki tikus ,istar ang "i in"uksi karagenan *

NPar Tests

One-Sample Kolmogorov-Smirnov Test

NILAI AUC

N 20

Normal Parametersa,bMean 179,7350

Std. De!at!on "0,"9203

Most #$treme D!%%eren&es

Absol'te ,1(7

Pos!t!e ,125

Ne)at!e *,1(7

+olmo)oro*Sm!rno ,-57

Asm/. S!). 2*ta!led ,7"1

Monte Carlo S!). 2*ta!led

S!). ,732&

95 Con%!den&e InteralLoer 4o'nd ,723

U//er 4o'nd ,7(0

a. est d!str!b't!on !s Normal.

b. Cal&'lated %rom data.

&. 4ased on 10000 sam/led tables !t6 start!n) seed 299""3525.


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Oneway

Descriptives

NILAI AUC

N Mean Std.

De!at!on

Std.

#rror

95 Con%!den&e Interal

%or Mean

M!n!m'

m

Ma$!m'

m

Loer

4o'nd

U//er

4o'nd

+N8L

N#AI:(

2"0,975

521,"597-

10,929"

"2(-,1917 315,7593 2-2,50 312,50

+N8L

PSII:

(110,3"(

5

71,9012-35,950-

3

*(,02-5 22(,7955 50,00 211,5(

:+D;P 150 (119,--5

025,029(-

12,51(7

379,"375 159,(925 93,75 153,--

:+D;P 300 (2(3,395

050,11121

25,055-

01-3,-5-9 323,1331 1"-,3- 297,22

:+D;P -00 (1((,255

02-,17(31

13,0"71

-102,-05" 1"5,90(2 110,71 1-9,15

otal 20179,735

0"0,"9203

1",0""0

11(1,"7-( 217,593- 50,00 312,50

Test of Homogeneity of Variances

NILAI AUC

Leene Stat!st!& d%1 d%2 S!).

1,9(" ( 15 ,155

ANOVA

NILAI AUC

S'm o% S


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Post Hoc Tests

Multiple omparisons

De/endent >ar!able? NILAI AUC

LSD

I +#LMP+ ; +#LMP+ Mean

D!%%eren&e I*;

Std. #rror S!). 95 Con%!den&e Interal

Loer 4o'nd U//er 4o'nd

+N8L N#AI:

+N8L PSII: 170,59101@ 30,77392 ,000 10(,9979 23-,1"(1

:+D;P 150 1-1,31051@ 30,77392 ,000 95,717( 22-,903-

:+D;P 300 37,5"051 30,77392 ,2(1 *2",012- 103,173-

:+D;P -00 13-,72051@ 30,77392 ,000 71,127( 202,313-

+N8L PSII:

+N8L N#AI: *170,59101@ 30,77392 ,000 *23-,1"(1 *10(,9979

:+D;P 150 *9,2"050 30,77392 ,7-7 *7(,"73- 5-,312-

:+D;P 300 *133,01050@ 30,77392 ,001 *19",-03- *-7,(17(

:+D;P -00 *33,"7050 30,77392 ,2"" *99,(-3- 31,722-

:+D;P 150

+N8L N#AI: *1-1,31051@ 30,77392 ,000 *22-,903- *95,717(

+N8L PSII: 9,2"050 30,77392 ,7-7 *5-,312- 7(,"73-

:+D;P 300 *123,73000@ 30,77392 ,001 *1"9,3231 *5",13-9

:+D;P -00 *2(,59000 30,77392 ,(37 *90,1"31 (1,0031

:+D;P 300

+N8L N#AI: *37,5"051 30,77392 ,2(1 *103,173- 2",012-

+N8L PSII: 133,01050@ 30,77392 ,001 -7,(17( 19",-03-

:+D;P 150 123,73000@ 30,77392 ,001 5",13-9 1"9,3231

:+D;P -00 99,1(000@ 30,77392 ,00- 33,5(-9 1-(,7331

:+D;P -00

+N8L N#AI: *13-,72051@ 30,77392 ,000 *202,313- *71,127(

+N8L PSII: 33,"7050 30,77392 ,2"" *31,722- 99,(-3-

:+D;P 150 2(,59000 30,77392 ,(37 *(1,0031 90,1"31

:+D;P 300 *99,1(000@ 30,77392 ,00- *1-(,7331 *33,5(-9

@. 6e mean d!%%eren&e !s s!)n!%!&ant at t6e 0.05 leel.


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Lampiran %I. 8asi anaisis statisti< DAI pa"a uji e&ek antiin&amasi &raksi k$r$&$rm

"aun J. curcas pa"a kaki tikus 'istar ang "i in"uksi karagenan *

NPar Tests


P#8S#NAS#

DAA

ANIIN:LAMASI

N 1-

Normal Parametersa,bMean (5,03"1

Std. De!at!on 2(,59-2"

Most #$treme D!%%eren&es Absol'te ,135Pos!t!e ,0"0

Ne)at!e *,135

+olmo)oro*Sm!rno ,539

Asm/. S!). 2*ta!led ,93(


S!). ,902&

95 Con%!den&e InteralLoer 4o'nd ,"9-

U//er 4o'nd ,907


b. Cal&'lated %rom data.&. 4ased on 10000 sam/led tables !t6 start!n) seed 92-21(("1.


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Oneway

Descriptives

P#8S#NAS# DAA ANIIN:LAMASI

N Mean Std.

De!at!on

Std.

#rror

95 Con%!den&e Interal %or

Mean

M!n!m'm Ma$!m'm


+N8L

PSII:( -0,7125 25,59052 12,7952- 19,9923 101,(327 2(,71 "2,20

:+D;P 150 ( 57,(100 ",907(" (,(537( (3,23-2 71,5"3" (5,31 --,-3

:+D;P 300 ( 13,3750 17,"3357 ",91-79 *15,0022 (1,7522 *5,7" 33,-7

:+D;P -00 ( (",-550 9,315"7 (,-5793 33,"31( -3,(7"- 39,79 -0,59

otal 1- (5,03"1 2(,59-2" -,1(907 31,9317 5",1((- *5,7" "2,20




1,72- 3 12 ,215

ANOVA


S'm o% S


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Post Hoc Tests

Multiple omparisons

De/endent >ar!able? P#8S#NAS# DAA ANIIN:LAMASI

LSD

I +#LMP+ ; +#LMP+ Mean

D!%%eren&e I*;



+N8L PSII:

:+D;P 150 3,30250 11,93231 ,7"7 *22,-95" 29,300"

:+D;P 300 (7,33750@ 11,93231 ,002 21,3392 73,335"

:+D;P -00 12,05750 11,93231 ,332 *13,9(0" 3",055"

:+D;P 150

+N8L PSII: *3,30250 11,93231 ,7"7 *29,300" 22,-95"

:+D;P 300 ((,03500@ 11,93231 ,003 1",03-7 70,0333

:+D;P -00 ",75500 11,93231 ,(77 *17,2(33 3(,7533

:+D;P 300

+N8L PSII: *(7,33750@ 11,93231 ,002 *73,335" *21,3392

:+D;P 150 *((,03500@ 11,93231 ,003 *70,0333 *1",03-7

:+D;P -00 *35,2"000@ 11,93231 ,012 *-1,27"3 *9,2"17

:+D;P -00

+N8L PSII: *12,05750 11,93231 ,332 *3",055" 13,9(0"

:+D;P 150 *",75500 11,93231 ,(77 *3(,7533 17,2(33

:+D;P 300 35,2"000@ 11,93231 ,012 9,2"17 -1,27"3



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Lampiran %II. Data !asi pengamatan !ist$pat$$gi juma! neutr$&i per // se

euk$sit pa"a masing-masing ke$mp$k k$ntr$ "an ke$mp$k perakuan

&raksi k$r$&r$m "aun J. curcas setiap jam seama jam pengamatan

ikus Juma! Neutr$&i ( =// se euk$sit)

Ke$mp$k

I

Ke$mp$k

II

Ke$mp$k

III

Ke$mp$k

I%

Ke$mp$k

%

) 1# # 3 1$ "

)) 11 # ! 13 5

))) 1$ 0 5 ! 5

Purata 2066 201 4 /01


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Lampiran %III. 8asi anaisis statistik pengamatan !ist$pat$$gi juma! neutr$&i

per // se euk$sit pa"a kaki tikus ,istar ang "i in"uksi

karagenan *


ne'tro%!l

N 15

Normal Parametersa,bMean 7,3333

Std. De!at!on (,1"-1(

Most #$treme D!%%eren&es

Absol'te ,17"

Pos!t!e ,17"

Ne)at!e *,1(3

+olmo)oro*Sm!rno ,-90

Asm/. S!). 2*ta!led ,729


S!). ,--1&

95 Con%!den&e InteralLoer 4o'nd ,-52

U//er 4o'nd ,-70


b. Cal&'lated %rom data.

&. 4ased on 10000 sam/led tables !t6 start!n) seed 2000000.


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Oneway

Descriptives

ne'tro%!l

N Mean Std.

De!at!on

Std.

#rror

95 Con%!den&e Interal %or

Mean

M!n!m'm Ma$!m'm


Bontrol

ne)at!% 3 12,3333 1,52753 ,""192 ",53"" 1-,1279 11,00 1(,00

Bontrol /os!t!% 3 2,---7 2,309(0 1,33333 *3,0702 ",(035 ,00 (,00

:+D;P 150 3 5,0000 2,00000 1,15(70 ,0317 9,9-"3 3,00 7,00

:+D;P 300 3 10,---7 3,21(55 1,"5592 2,-"13 1",-521 7,00 13,00

:+D;P -00 3 -,0000 1,73205 1,00000 1,-973 10,3027 5,00 ",00

otal 15 7,3333 (,1"-1( 1,0"0"- 5,0151 9,-515 ,00 1(,00


ne'tro%!l


1,027 ( 10 ,(39

ANOVA

ne'tro%!l

S'm o% S


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Post Hoc Tests

Multiple omparisons

De/endent >ar!able? ne'tro%!l

LSD

I Belom/oB ; Belom/oB Mean D!%%eren&e

I*;



Bontrol ne)at!%

Bontrol /os!t!% 9,----7@ 1,"257( ,000 5,59"7 13,73(7

:+D;P 150 7,33333@ 1,"257( ,002 3,2-53 11,(013

:+D;P 300 1,----7 1,"257( ,3"3 *2,(013 5,73(7

:+D;P -00 -,33333@ 1,"257( ,00- 2,2-53 10,(013

Bontrol /os!t!%

Bontrol ne)at!% *9,----7@ 1,"257( ,000 *13,73(7 *5,59"7

:+D;P 150 *2,33333 1,"257( ,230 *-,(013 1,73(7

:+D;P 300 *",00000@ 1,"257( ,001 *12,0-"0 *3,9320

:+D;P -00 *3,33333 1,"257( ,09" *7,(013 ,73(7

:+D;P 150

Bontrol ne)at!% *7,33333@ 1,"257( ,002 *11,(013 *3,2-53

Bontrol /os!t!% 2,33333 1,"257( ,230 *1,73(7 -,(013

:+D;P 300 *5,----7@ 1,"257( ,011 *9,73(7 *1,59"7

:+D;P -00 *1,00000 1,"257( ,59- *5,0-"0 3,0-"0

:+D;P 300

Bontrol ne)at!% *1,----7 1,"257( ,3"3 *5,73(7 2,(013

Bontrol /os!t!% ",00000@ 1,"257( ,001 3,9320 12,0-"0

:+D;P 150 5,----7@ 1,"257( ,011 1,59"7 9,73(7

:+D;P -00 (,----7@ 1,"257( ,029 ,59"7 ",73(7

:+D;P -00

Bontrol ne)at!% *-,33333@ 1,"257( ,00- *10,(013 *2,2-53

Bontrol /os!t!% 3,33333 1,"257( ,09" *,73(7 7,(013

:+D;P 150 1,00000 1,"257( ,59- *3,0-"0 5,0-"0

:+D;P 300 *(,----7@ 1,"257( ,029 *",73(7 *,59"7



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Lampiran I>. D$kumentasi Peneitian

Pr$ses penguapan Pemberian Karagenan

Penaringan &itrat

pemberian se"iaan uji

Ekstrak kenta &raksi

k$r$&$rm

pengukuruan ;$ume e"ema

Kaki tikus untuk preparat 8E

Se"iaan uji ?KDJP berbagai "$sis

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Lampiran Fix Print