a. Address here.b. Address here.c. Address here.† Footnotes relating to the title and/or authors should appear here.Electronic Supplementary Information (ESI) available: [details of any supplementary information available should be included here].

Journal of Scientific Research

Received 00th January 20xx,Accepted 00th January 20xx

www.bhu.ac.in/research_pub/jsr/

Title

X. Y. Authornamea, X. Y. Authornameb and X. Y. Authornamec*

Abstract: text goes here. The abstract should be a single paragraph that summarises the content of the article.

Two acid hydrazones incorporating xanthene as a metal chelating centre (B-XAN and N-XAN)have been synthesized, characterized and evaluated for the optical sensing of Mg2+ and Al3+. The replacement of naphthyl moietyin N-XANby phenyl moietyin B-XANleads tototal specificity of the later one towards Mg2+.The B-XANexhibited a highly sensitive and selective turn ‘on’ fluorescent response towards Mg2+through intense blue fluorescence enhacement. This measurment was free from any interference by other cationic analytes including the Ca2+ and Al3+ also. The binding constant, stoichiometry as well as the mechanistic aspect of the above sensing phenomenon were also studied through various spectroscopic methods.

Keyworws: First; Second; Third; Fourth; Fifth......

A Headings are the primary heading type e.g. Introduction, Results and discussion, Experimental and Conclusion.

B Headings should always be subordinate to A headings e.g. Synthetic procedures, Materialsand methods, Crystallography.

C headings should always be subordinate to B headings e.g. General procedure for synthesis of compound X. The main paragraph text follows directly on here.

The main text of the article should appear here with headings as appropriate.

The name of the corresponding author must be indicated with star*.

Introduction

In recent years, myriads of fluorescent chemosensors for Mg2+

have been reported due to their biological, analytical as well as environmental3importance. The magnesium is the second plentiful intracellular cation and the fourth most abundant cation in human body. Mg2+ is indispensable for mediating many enzyme-catalysed reactions participating in many biological functions such as, a cofactor in the phosphorylation of glucose during carbohydrate metabolism, proper functioning of nerves and immune systems, muscle and bone remodelling and skeletal development. Furthermore, it has also been found to be involved in modulation of signal transduction, various transporters, ion channels and stabilization of DNA conformation etc.

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(a)

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Journal of Scientific Research

(b)Fig. 2(a) Single crystal of B-XAN with50 % probability.(b) Supramolecular architecture showing intra and intermolecular hydrogen bondings in B-XAN.

TABLE I. Table Type Styles

Table Head

Table Column HeadTable column subhead Subhead Subhead

copy More table copya

( x+a )n=∑k=0

n

(nk )xk an−k (1)

Results and discussion

The synthetic pathways of two new fluorogenic xanthene based derivatives B-XAN[(E)-N,-(2-hydroxybenzylidine) -9H-xanthene-9-carbohydrazone] and N-XAN[(E)-N'-((2-hydroxynaphthalen-1-yl)methylene)-9H-xanthene- 9-carbohydrazide] are shown in Scheme1.The corresponding aldehydes such as 2-hydroxy benzaldehyde and 2-hydroxy naphthaldehyde were added separately to the 20 mL methanolic solution of 9H-xanthene-9-carbohydrazide at room temperature with constant stirring for ~4-5 hours resulting into white and light yellow solids respectively which were filtered, washed and dried under vacuum. These probes were fully characterized (see the experimental section).

Experimental

The syntheses of B-XAN and N-XAN have been shown in Scheme1. The 2.0mM absolute methanolic solution of 2-hydroxybenzaldehyde and 2-hydroxynapthaldehyde was added to an equimolar absolute methanolic solution of 9H-xanthene-9-carbohydrazide respectively at room temperature with constant stirring for ~4-5 hours. As a result of this, white and light yellow solids were precipitated respectively which were finally dried under vacuum over anhydrous CaCl2. B-XANand N-XAN were characterized through various spectroscopic techniques like IR, 1H &13C NMR spectral studies along with ESI-mass determination (Fig.S1-S8)and single crystal XRD analysis (Table 1).

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ReferencesThe template will number citations consecutively within brackets [1]. The sentence punctuation follows the bracket [2]. Refer simply to the reference number, as in [3]—do not use “Ref. [3]” or “reference [3]” except at the beginning of a sentence: “Reference [3] was the first...”Number footnotes separately in superscripts. Place the actual footnote at the bottom of the column in which it was cited. Do not put footnotes in the reference list. Use letters for table footnotes.Unless there are six authors or more give all authors’ names; do not use “et al.”. Papers that have not been published, even if they have been submitted for publication, should be cited as “unpublished” [4]. Papers that have been accepted for publication should be cited as “in press” [5]. Capitalize only the first word in a paper title, except for proper nouns and element symbols.For papers published in translation journals, please give the English citation first, followed by the original foreign-language citation [6].

[1] G. Eason, B. Noble, and I. N. Sneddon, “On certain integrals of Lipschitz-Hankel type involving products of Bessel functions,” Phil. Trans. Roy. Soc. London, vol. A247, pp. 529–551, April 1955. (references)

[2] J. Clerk Maxwell, A Treatise on Electricity and Magnetism, 3rd ed., vol. 2. Oxford: Clarendon, 1892, pp.68–73.

[3] I. S. Jacobs and C. P. Bean, “Fine particles, thin films and exchange anisotropy,” in Magnetism, vol. III, G. T. Rado and H. Suhl, Eds. New York: Academic, 1963, pp. 271–350.

[4] K. Elissa, “Title of paper if known,” unpublished.[5] R. Nicole, “Title of paper with only first word capitalized,”

J. Name Stand. Abbrev., in press.[6] Y. Yorozu, M. Hirano, K. Oka, and Y. Tagawa, “Electron

spectroscopy studies on magneto-optical media and plastic substrate interface,” IEEE Transl. J. Magn. Japan, vol. 2, pp. 740–741, August 1987 [Digests 9th Annual Conf. Magnetics Japan, p. 301, 1982].

[7] M. Young, The Technical Writer’s Handbook. Mill Valley, CA: University Science, 1989.

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