LUCIFERASE

A. Cengiz Öztireli

BIOLUMINESCENCE

BIOLUMINESCENCE (LIVING LIGHT)Milky Sea

BIOLUMINESCENCE

Applications

BIOLUMINESCENCE

Sea AnemonesBacteria Dinoflagellates

BIOLUMINESCENCE

FIREFLYES (PHOTINUS)Attracting matesAttracting prey

LUCIFERASE

FIREFLY BACTERIA

BIOLUMINESCENCE

8 ATP → 1 Green Photon

Reaction:

LuciferinLuciferase + O2 Inter. P* P + hv

ATP

BIOLUMINESCENCE

Color P*

P* + F → F* + P Proteins around Structure

PHOTINUS PYRALIS (FIREFLY)

PHOTINUS PYRALIS LUCIFERASE

Luciferase + Luc-COOH

ATP

Luciferase: Luc-CO-AMP

O2

Luciferase: Luc=O*

PHOTINUS PYRALIS LUCIFERASE

N-Terminal

C-Terminal

Cleft

Structure and Functioning

CONCLUSIONS

THANK YOU