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Proposed Syllabus

For

B.Tech. Bio -Technology Course

Of

M.D University

(Board of studies in Technology)

(2006)

Note: The scheme of Examination and the content of syllabi for 1st and 2nd semester of B.Tech Biotechnology will be same as of B.Tech Group-I (El/EIC/ECE) Branches.

M.D.UNIVERSITY, ROHTAK

SCHEME OF STUDIES & EXAMINATION

2ND YEAR B. TECH. IN BIOTECHNOLOGY, SEMESTER III

EFFECTIVE FROM THE SESSION 2006-07

S.No

Course No.

Subject

Teaching Schedule

L T P/D Total

Examination Schedule

Th. Sess P/VV Total

Duration

of exam

1.

BT 201

Cell Biology

3 1 - 4

100 50 - 150

3

2.

BT 203

Micro biology

3 1 - 4

100 50 - 150

3

3.

BT 205

Bio chemistry

3 1 - 4

100 50 - 150

3

4.

BT 207

Genetics

3 1 - 4

100 50 - 150

3

5.

BT- 209

Organic Chemistry

3 1 4

100 50 - 150

6.

BT 211

Mol. Biol.

3 1 - 4

100 50 - 150

3

7.

BT 213

Cell Biology & Genetics Lab

- - 3 3

- 50 50 100

3

8

BT 215

Microbiology Lab.

- - 3 3

- 50 50 100

3

9.

BT 217

Biochem. & Mol Biol. lab

- - 3 3

- 50 50 100

3

10.

BT 219

Organic Chemistry lab

- - 2 2

- 25 25 50

3

Total

18 6 11 35

600 445 205 1250

Note 1.Students are allowed to use single memory, non-programmable scientific calculator during exam.

3rd SEMESTER( Bio Tech. )

CELL BIOLOGY

BT 201

L T Theory: 100 Marks

3 1 Sessional: 50 Marks

Total: 150 Marks

Time: 3 Hrs.

UNIT I

1. Cell: An introduction and classification of organisms by cell structure,cytosol, compartmentation of eukaryotic cells, cell fractionation.

2. Cell membrane and Permeability: Chemical components of biological membranes , organization and fluidity of membrane components, the membrane as a dynamic entity of cell, signalling ,cell recognition and membrane transport.

UNIT II

3. Membrane Vacuolar System Cytoskeleton and cell motility: Structure and function of microtubules,Microfilaments, Intermediate filaments .

Endoplasmic reticulum: Structure, function including role in protein segregation.

Golgi complex: Structure, biogenesis and functions including role in protein secretion

Lysosomes , vacuoles and microbodies : Structure and functions.

4. Ribosomes : Structure and function including role in protein synthesis.

5. Mitochondria : Structure , genomes , biogenesis .

6. Chloroplasts : Structure , genomes, biogenesis.

UNIT III

7. Nucleus : Structure , cell cycle (Interphase & M phases ), regulation of cell cycle.

8. Extracellular matrix : composition ,molecules that mediate cell adhesion, membrane receptors for extra cellular matrix, macromolecules ,regulation of receptor expression and function.

9. Muscle contraction:Different muscle type in the body, structural protein of muscles, energetics and regulation of muscle contraction .

10.Neurons and Neurotransmission : Resting potential, Action potential , Synaptic transmission neurotransmitters and receptors ,the generation of action potential by sensory stimuli and mechanism of nerve-impulses

11. Cancer : Carcinogenesis , agents promoting carcinogenesis, characteristics of cancer cells,molecular basis of cancer.

TEXT / REFERENCE BOOKS

1. Molecular Biology of cell, 3rd edition by Albert et al, Garland Publishing ,Inc.

2. Cell Biology Smith and Wood by Chapman and Hall

3. Cell Biology: Organelle structure and function by D.E .Sadava, Jones & Bartlett Publishers.

4. Cell and Molecular Biology , 8th ed. EDP. De Robertis and EMF DeRoberties, Lippincot

Williams & Wilkins Pvt Ltd , ( international student edition).

5. Molecular cell Biology Harvey Lodish and D .Baltimore W.H Freeman & Co. 4th ed.2000.

Note for Paper setter:

Eight questions will be set in the question paper .Two from unit 1 & three each from unit II & III. Candidates will be required to attempt five questions, selecting at least one from each unit.

3rd SEMESTER B.Tech. (Bio Tech. )

MICRO BIOLOGY

BT- 203

L T Theory : 100 Marks

3 1 Sessional : 50 Marks

Total : 150 Marks

Time : 3 Hrs.

UNIT I

Fundamentals of Microbiology: Scope and relevance of microbiology, Evolution of microbiology and its history.

Classification of Microorganisms : Microbial Taxonomy, Criteria used including molecular approaches, Microbial phylogeny and Current classification of bacteria.

.

Microbial Diversity- Distribution & Characterization : Prokaryotic and Eukaryotic cells. Morphology and cell structure of major groups of microorganisms.e.g. bacteria, Fungi, algae, protozoa. Unique features of viruses.

UNIT II

Cultivation & Maintenance of Microorganism Nutritional categories of microorganisms. Methods of isolation, Purification and preservation.

Microbial Growth and Metabolism - Growth curve, generation time. Synchronous batch and Continuous culture. Measurement of growth and factors affecting growth . Metabolic pathways amphicatabolic and biosynthetic.

Bacterial reproduction Transformation , Transduction & conjugation. Endospores, sporulation.

UNIT III

Control of Microorganism: Physical & chemical agents.

Water microbiology Bacterial pollutants of water, the coliform group . Sewage composition and its disposal.

Food microbiology Important Microorganism in food microbiology molds, yeasts & bacteria. Major food born infections and intoxications, preservation of various types of foods.

TEXT / REFFERENCES BOOKS

1. Microbiology: Prescott et al., 2003, 5th edition, Mc Graw Hill, USA.

2. Microbiology: Pelczar et al, Tata Mc Graw Hill, New Delhi.

3. Food Microbiology: Adams & Moss, 1996, C B S Publishers, New Delhi.

Note for paper setter:

Eight questions will be set in the question paper. Atleast two from each unit. Candidates will be required to attempt five questions, selecting at least one from each unit.

3rd SEMESTER B.Tech. (Bio Tech. )

BIOCHEMISTRY

BT- 205

L T Theory : 100 Marks

3 1 Sessional : 50 Marks

Total : 150 Marks

Time : 3 hrs.

UNIT I

1. Introduction to Biochemistry: A historical prospective.

2. Amino acids & Proteins Structure & Function. Structure and properties of Amino acids, sources of amino acid pool. General reactions of amino acids: Transamination, Oxidative and non- oxidative deamination & decarboxylation. Peptide bond, types of protein and their classification, acid base properties and Titration of proteins, Forces stabilizing protein structure and shape.

Different Level of structural organization of proteins, Purification of proteins and criteria of their purity. Denaturation and renaturation of proteins. Fibrous and globular proteins.

3. Carbohydrates Structure and function : Structure and properties of Monosaccharides, oligosaccharides and polysaccharides. Ring stru