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n 1990, we began to use computerized methods in my laboratory, in the city of Aguascalientes, to study the three main causes of blindness in Mexico. These meth- ods allowed us to study the retina and the optical nerve in living beings in a very similar way to the technique known as spectrophotometry, in which a specific wavelength in applied to the retina and nerve and a characteristic image or response is obtained. played by melanin, a substance also known chemically as polihydroxyindol, which has extraordinary properties and all of them tend to do something to protect the tissues, but none of them seemed to explain such constant and complete protection. We have achieved, for the first time, alternative electricity generation using self-  renewing photoelectrochemical cells which separate the hydrogen atom from water  and then bring the atoms together again. This is a fundamental scientific break-  through that could bring us closer to the hydrogen era. Melanin: ¿fuel of the future? ARTURO SOLÍS HERRERA * * Mr. Solis Herrera graduated as a medical surgeon from the National Polytechnical Institute (IPN), as an ophthalmologist from the National Autonomous University of Mexico (UNAM) and as a neuro-ophthalmologist from the National Institute of Neurology and Neurosurgery (INNN). He is a master of medical sciences from the Aguascalientes Autonomous University and doctor in pharma- cology from the University of Guadalajara. Currently, he works as technical director at the chemical and pharmaceutical company Formulas Magistrales in the city of Aguascalientes. Fig 1. In this picture we see a detailed image of the optical nerve, which is the circular structure, which measures the equivalent of 12 human hairs held together. Upon changing the wavelength and/or using filters and means of contrast, as a kind of radar for penetration, we have obtained other images that give us important information for evaluating eye disease. During this research, we detected the important role We were able to extract the first 20 mililiters of melanin for the first time in 1998, since my idea was that if we could instill it in the human eye, we could achieve reasonable therapeutic ef- fects. The results we did in fact achieve were very impressive, indeed much better than we expected.    M    o    l    e    c    u    l    a    r    C    e    l    l    B    i    o    l    o    g    y  ,    L    o    d    i    s    h  ,    B    e    r    k    ;    E    d  .    F    r    e    e    m    a    n Fig 2. Pho tosynthes is i n plants.

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It took us 12 years, from 1990 to 2002, to understand howthis substance worked so effectively. Finally, we could confirmour hypothesis –which was incredible even to ourselves– that mela-nin delivers hydrogen to the cell. In other words, it captures pho-tonic energy and transforms it into chemical energy. This aston-ished us, given that hydrogen is the smallest atom of all, the mostabundant in the universe and it is the carrier of energy that natureuses most.

We can define photosynthesis as the absorption of photonsfrom electromagnetic radiation, which brings about an ionic event.Until today, chlorophyll is accepted to be the only substance widelydisseminated in nature that is capable of delivering hydrogen to aplant cell.

No other substance has been known in eucaryote cells (inmammals, fish, birds, insects, etc.) that, by capturing photons from

electromagnetic radiation, can get the necessary energy to splitthe water molecule. Results obtained using melanin confirm to usthat not only plants carry out photosynthesis, but that mammalscan also do so. Indeed, so can any living being that has melanin inits genetic code. In other words, melanin is to the animal king-dom what chlorophyll is to the plant kingdom.

As can be seen in Fig.3, both compounds have certain simi-larities, particularly the four nitrogen atoms in the center. N thecase of melanin, it would appear that Nature made asuperchlorophyll, since chlorophyll has only one center of reac-

tion, while chlorophyll has hundreds of centers of reaction pergram of the substance.

In several research institutes, attempts have been made tomake use of chlorophyll’s ability to split the water molecule inorder to obtain hydrogen for energy purposes. However, it turnsout that once chlorophyll is taken out of the leaf of a plant, itbecomes permanently inactive 20 seconds later. The Universityof California has been trying to improve chlorophyll’s activitylevel outside of plants for the past 50 years, without achievinguseful results.

This fact led us to doubt the usefulness of our research. If wetake melanin out of the tissue and make it produce energy, howlong is the reaction going to last? Maybe 30 seconds or 50 sec-onds? To our surprise, it functions for years and, if we perfect thetechnology, it might very well function for decades or maybe evenhundreds of years. In other words, melanin is many thousands of times more efficient in capturing particles of electromagnetic ra-diation (photons) than is chlorophyll.

The question was: How does melanin extract energy fromwater? In the water molecule, energy is extracted by separat-ing and reuniting the hydrogen atoms from the oxygen at-oms.

A

B

Fig 3. A) Proposed (theoretical) formula of melanin.  B) Formula of chlorophyll.

Fig. 4 A) Spectrum of absorption of chlorophyll, with its peaks of absorption at 450and at 650 manometers.

  B) Electromagnetic spectrum of melanin, in which greater amplitude andefficiency is observed, as it absorbs thousands of times more photons than doeschlorophyll

A

B

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Energy is produced from water in line with the followingreaction (¤ = light).

The reaction outlines above shows that two water molecules,plus melanin, in the presence of photons of electromagnetic ra-diation (symbolized by the sun), gives us tow hydrogen molecules,an oxygen molecule and four electrons. However, when the reac-tion goes from right to left, the hydrogen and oxygen atoms re-unite, giving us water and electricity, since the melanin does notundergo change, since it only supports and catalyzes the reactionwithout any deterioration to its molecule. The arrow indicates thatthe reaction goes in both directions and, being complementaryreactions, one exergonic and the other endergonic, a cycle is es-tablished which lasts for years, as the melanin does not deterio-rate. The melanin captures photonic energy and uses it to extractthe hydrogen molecule from water. The time needed to recollectthe energy required to split the water molecule is 3 x 10-12 sec-onds.

We have estimated that the third of all energy usually avail-

able to a human being comes from melanin, light and water. How-ever, this third of all energy is the primordial energy, that is, it isthe energy that activates chemical reactions in the human organ-ism. In line with our therapeutic studies (whose results have beenextraordinary), life systems are supported by this energy one wayor another, or even require chemical energy from melanin derivedfrom photonic energy in order to begin or to sustain life. This iscoherent with clinical findings related to the time that a humanbeing can live without water. Water is accepted to be the univer-sal solvent and diluting agent, but if we add that it is also, in the

presence of light and melanin, the source of a third of total energyused by the human body, and that this energy is key to beginningmost important processes, such as sight, it can be more easilyunderstood that a human being can tolerate the lack of water foronly three days, while he can go without food for up to three weeks.

We have compared this molecule in the human being to mela-

nin molecules in other species and we were surprised to find thatthe molecule is the same in all species. NASA defines life as aself-sustaining chemical system, capable of evolving in a darwinianmanner. Melanin is, without doubt, a very important precursor of life.

In January 2005, I listened to a speech by George W. Bush,President of the United States, who said that substances are neededthat can separate the hydrogen atom from water, so that we canfully enter into the hydrogen era. I wondered: Why don’t they usemelanin? I set about the task of finding the answer and I did find

Fig. 5. Presence of melanin asseen in some plants, for in-stance, in two eggplants.Melanin is part of geneticcode of one of them, but notof the other. It is undeniablethat the plant that can ab-sorb electromagnetic radia-tion receives an extra sup-ply of energy giving it phe-nomenal advantages in life.

Fig. 6. Image of the human eye 35 days into pregnancy. The fetus’ eye iscompletely full of melanin, a substance that is so important for life,that it forms immediately and provides energy to the tissues, so thatall kinds of reactions can take place that lead to life.

Photographs book: Nacer, l a gran aventura. Lennart Nilsson, Salvat.

Fig. 7. Approximatethree-dimen-sional structureof melanin.

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it. They don’t use melanin, because, apart from myself, nobodyelse knew about it. Without giving it much thought, I decided, inJune 2005, to begin proceedings on the patent: “New

 photoelectrochemical procedure to break the water molecule intohydrogen and oxygen using as the main substrate melanins, their 

  precursors, analogues or derivatives”.

GENERATING ELECTRICITY 

Another fundamental feature of melanin is its stability inwater. This is vital for electricity generation. For instance, thefirst sample that we were able to extract in January 1998 began itsninth year without any kind of deterioration in January 2007. Thefirst cells (prototypes) we made with a view to generating elec-tricity, this year began their sixth semester producing electricitywithout interruption at room temperature.

In Fig. 8, a voltmeter registering zero current can be seen, asthe recipient does not contain a melanin solution. In contrast, inFig. 9, the voltmeter records 300 milivolts or as much as 470

milivolts when the concentration of melanin is increased. More-over, once the cell is sealed, the cell does not require rechargingin any way.

On March 13, 2007, we managed to light the first light emit-ting diode (LED), which remains lit six months later and lightsour laboratory day and night. Our cells are still very elemental,but they do produce electricity and we are working on makingthem more efficient and scaling them up to competitive costs. Ini-tially, we used a concentration of 1.3% melanin and 98.7% water.Later, when we increased the concentration of melanin to 4%, the

generation of electricity increased exponentially.In terms of technological development, we have achieved

progress I consider to be significant and which can reflect thepotential of such cells. For instance, early in 2006, we were pro-

ducing a liter and a half of melanin every three months and ourcells were of 30 mL and produced 400 mV and 10 uA. In March2007, when we were able to light a LED for the first time, thecells we were making were of 500 mL and produced 500 mV and200 uA. By this time, we could produce about 50 liters of melanindaily. The modules we used for demonstration allowed us to lighta LED with ten 500 mL cells.

More recently, we were able to connect up a small musicplayer, since each cell now produces 600 mV and 200 mA, that is,a thousand times more than the 200 uA we used to achieve. Cur-rently, in our small laboratory, we produce about 200 liters of melanin daily.

The perspectives, for now, mainly have to do with lighting.We would hope to do lighting on a more massive scale within twoyears and to have the right kind of designs for that. Within fiveyears, we think it might be feasible to put together a prototypevehicle and the most interesting aspect of this would be that thisvehicle would never have to go into a gasoline station. We willneed economic support to integrate interdisciplinary teams to scaleup this fuel-cell technology in an effective and rapid manner, sincewe are currently exercising our professions and carrying out thisresearch simultaneously.

Fig 8. Prototype of aself-renewingphotoelectroche-mical cellconnected to a

voltmeter,allowing for measurementof changes inelectricpotential, whenusing melanin(empty cell).

Fig 9. Cell with melanin.

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It should be taken into account that several European na-tions, as web as the United States, say they have budgeted 100billion dollars for the possible future construction of “hydrogasoline stations,” that is, stations that will sell hydrogenfor vehicles. At this time, they do not know where they will get

the hydrogen from, because, for now, hydrogen can only be ob-tained from gas or from oil.

In line with our current knowledge, 1,000 liters of melaninprovide 10,000 volts, plus miliamperes, but these figures can bemodified to specific needs, that is, it can be modulated accordingto the size of the cells, the way the cells are connected to eachother, the size and the arrangement of the electrodes, modifica-tions in the formula of the central substrate, etc. In other words,the possibilities are almost infinite and much work will be re-quired to discover and develop them. As Vladimir S. Bagotsky

says on page 22 of his book, Fundamentals of Electrochemistry,published by Wiley (second edition, 2007), in designing electro-chemical systems, it is impossible to predict how they will workout and all options have to be tested.

RESPONDING TO SKEPTICISM

Our research has met with some skepticism when we havepresented it to different people, since the concept that photosyn-thesis occurs only in plants, and not in mammals, is very deeplyingrained in people’s minds.

However, in March 2007, the article “Ionizing RadiationChanges the Electronic Properties of Melanin and Enhances theGrowth of Melanized Fungi”, was published by EkaterinaDadachova y her colleagues at the Albert Einstein College of Medicine, Nueva York (PLoS ONE 2(5): e457. doi:10.1371/ 

 journal.pone.0000457). This article is very important, because,finally, an independent research team has made findings that arecompatible with our theory that melanin has the ability to carryout photosynthesis, also reaching this conclusión by observingthe biological effects of melanin. This is explainable in the sensethat melanin is accepted to be “intractable” (The Physical and 

Chemical Properties of Eumelanin, Paul Meredith and TadeuszSarna, 2006, Blackwell Munksgaard doi: 10.1111/j.1600-0749.2006.00345), which refers to the fact that it has been impos-sible to discern the chemical structure of melanin. If we could doso, this would allow us to infer or explain if not all, at least some,of its extraordinary physical and chemical properties. Meredith

A

B

Fig 10. A) Osciloscope concected to the cell.  B ) LED lit by the cell.

Fig.11 LED lit by cell.

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reaffirms the great difficulty in studying melanin and states a veryconcrete question: Where does the enormous amount of photonicenergy go, that is absorbed by melanin?

Our findings are based on the observation of the biologicaleffects of melanin on the human retina and may still be contro-versial. However, we have been progressing both in the develop-ment of applications of alternative electricity generation and inthe production of medicines that modulate photosynthesis in hu-man beings, paying attention, of course, to all the bioethical andlegal aspects that are implicit. We began proceedings on anotherpatent in 2006 based on the extraordinary therapeutical benefits.

In the article by Dadachova and colleagues, the observationsin their research are compared to the way plants obtain energyfrom photosynthesis. Their findings should contribute to morerapid acceptance of the results of our research, allowing scien-tists, government officials and businesspeople to make better de-cisions regarding integrating multidisciplinary teams that can de-velop, as quickly as possible, all applications, both in the field of energy and in the area of medical pharmacology. In the latter field,

the pharmacological modulation of photosynthesis in human be-ings offers major expectations in ailments such as Alzheimer, dif-ferent types of arthritis, nephropathy, enteropathy, sepsis, etc.

LOOKING TO THE FUTURE

Finally, one must underline the growing global concern onclimate change. Global warming trends are gaining momentumand it would appear that we are heading towards a disaster situa-tion, if the trends are not reversed. The imminence of catastro-phes, if rapid action is not taken, will go beyond the ability of governments and peoples to mitigate and face this phenomenonand its consequences, experts say. Signs are that climate changeis outstripping the measures foreseen in the Kyoto Protocol.

Faced with this reality, the development of solutions basedon melanin may be able to offer a light at the end of the tunnel.. Ibelieve it is worthwhile studying this and developing it seriously.

Possibly a national research project could be carried out that couldgo beyond our borders. The demand for an efficient, self-renew-ing photoelectrochemical cell, from a cost-benefit viewpoint, couldbe overwhelming.

It is something that I personally could hardly expect toachieve in the short term. That is why I am at a stage of seekingsupport, not only financially, but strategically, logistically and tech-nologically, because a task of this scope requires many brilliantminds, each working in their own direction. But it is a possibilitythat should be within our reach.

B

A

Fig. 12 A) Work table with several cells connected.  B) Group of LEDS lit by cells.

Fig. 13. Optical nerve.