• Metadata– Date (at beginning and end of

data collection) (year, month, date)

– Growing season? (yes or no)– GPS location (in decimal

degrees)– In situ or pot?– Species ID (family, genus,

species)– Plant functional type– Growth form– C3/C4/Cyad– Angiosperm/ gymnosperm– Deciduous/evergreen– Contributor– Citation– Site ID

– Root order– Functional classification– Soil depth ( upper and

lower) (cm)– Depth of field (mm)– Treatment, extent of

treatment, and unit for extent

– Collection method– Soil block dimensions

(length, width, height) (cm)– Calculation method– Latitude, longitude– Stand age (yr)– Location notes– notes

• Morphology– Root diameter (mm)– Specific root length (SRL) (m

g-1)– Root tissue density (RTD) (g

cm-1)– Branching intensity (# roots

per higher order root)– Branching architecture (root

length per higher order root)– Type of mycorrhizae (AM or

EM)– % EM colonization (% tips

colonized)– % AM colonization (% length

colonized)

• Anatomy– Stele diameter (µm)– Cortex diameter (µm)– Root porosity (aerenchyma

area)– Vessel diameter (µm)– Phellem

• Standing Biomass– Absolute biomass (g m-2)– % total biomass (by order,

diameter, and functional class)

– Annual production (g m-2)– Annual mortality (g m-2 year-1)– Biomass turnover (g m-2a-1)

• Chemistry– Root C, N, K, P, Al, Ca, Mg

(mg g-1)– Root Cr, Ni, Cu, Zn, As, Cd,

Pb (mg kg-1)– C:N ratio– C storage (kg C hm-2)– Ion concentration (Na+ and

Cl-) (mg g d.wt-1)– Lignin– Cellulose– Hemi-cellulose– Carbohydrates (TNC)(mg g-1)– Isotopic concentration

Mean root stable δ15N (%)

• Dynamics– Survivorship (roots year-1)– Growing season survivorship (%

growing season-1)– Phenology production– Phenology mortality– Lifespan (days)– Turnover rate (% growing season-

1)

• Function– Respiration rate (nmol O2 g-1 s-1)– Respiration rate by mass (nmol s-1

m-2)– Nutrient uptake– Water uptake– Exudate rate– Oxygenation rate– Enzyme production– Conductance

• Soil dynamics– Soil temperature (°C)– Volumetric soil water content

(%)– Oxygen availability– Soil respiration– Mineralization rate

• Climate– Mean annual precipitation (mm)– Min & max monthly

temperature (°C)– Mean annual temperature (°C)– Chamber temperature (day,

night) (°C)

• Other site characteristics– Elevation (m above sea level)– Slope (°)

• Soils– pH– Textural class– Soil horizon– % sand, silt, clay, and SOM– Bulk density (g cm-3)– Soil N and P (g kg-1)– Al:Ca ratio– Cation exchange capacity (CEC)

(meq cg-1)– % base cation saturation– Elemental concentration (total

and exchangeable)– Redox potential (mV)

• Vegetation– Basal area (total and by species)– LAI (total and by species)– Photosynthesis