Module Guide for the Study Path - uni-luebeck.de Guide for the Study Path ... Viva Voce written or oral ... Can present a molecular virology based paper in a journal club format

Module Guide for the Study Path

Master Infection Biology

Version from 6. November 2015

Module Guide

Infection Biology Infection Biology 1 (LS4015, InfBiol1) 1

Immunology (LS4035, Immunol) 3

Infection Biology 2 (LS4145, InfBiol2) 5

Medical Microbiology (LS4175, MedMicro) 7

Module part: Molecular Virology (LS4175 A, MedMicroVi) 8

Module part: Mechanisms of Bacterial Pathogenicity (LS4175 B, MedMicroBa) 9

Module part: Pathogen Niches (LS4175 C, MedMicroNi) 10

Module part: Inflammation - Methods in Immunology (LS4175 D, MedMicroIn) 11

Cellular and molecular Microbiology Module part: Crystallography (LS4020 A, StrAnaKris) 13

Module part: NMR Spectroscopy (LS4020 B, StrAnaNMR) 14

Module part: Single Molecule Methods (LS4020 C, Einzelstru) 15

Module part: Microscopy: techniques and applications (LS4020 D, StrAnaMikr) 16

Module part: Basic Membrane Biophysics (LS4020 E, MemBiophy) 17

Module part: Protein-Biophysics (LS4020 F, ProBioPhy2) 18

Structural Biology of Infection (LS4020-IB, StrucBiol) 19

Diagnosis of Infectious Diseases (LS4045, DiagInfDis) 20

Anti-Microbial Therapy and Prophylaxis (LS4155, AntTherPro) 22

Host-Pathogen Interaction (LS4185, HostPatInt) 23

Module part: Analysis of Host Pathogen Interaction (LS4185 A, AnalHPI) 24

Module part: Rational Drug Design (LS4185 B, RatDruDes) 25

Competencies and clinical aspects Module part: Introduction to Bioinformatics (CS1400 T, Bioinfo) 26

Bioinformatics, Modelling and Biostatistics (CS4011, BioModEpi) 27

Clinical Aspects of Infection (LS4025, ClinAsp) 28

Model Systems of Infection (LS4165, ModSysInf) 30

Module part: Genetic Epidemiology (MA3200 T, GenEpi) 31

Ethics in Sciences / Scientific Writing (PS4610, EthScWr) 32

Module part: Ethics in Sciences (PS4610 A, Ethics) 33

Module part: Scientific Writing (PS4610 B, SciWrit) 34

Interdisciplinary internships and consolidation courses Practical Course (LS4115, PC) 35

Module part: Project Management (LS5200 PM, ProjectM) 36

http://www.uni-luebeck.de/

Module Guide

Modul part: Lipidomics and biological mass spectrometry (LS5200 SA, Massenspek) 37

Module part: Biochemistry of the bacterial envelope (LS5200 SB, BacEnv) 38

Module part: Molecular Dynamics (LS5200 SD, MolDyn) 39

Module part: Structural Aspects of Protein biosynthesis (LS5200 SF, ProtBSynth) 40

Module part: Light into Darkness: Modern Fluorescence Techniques in Structural Biology (LS5200 SH, ModernF) 41

Module part: Characterization of Protein Complexes in Structural Biology (LS5200 SI, ProtCompl) 42

Module part: Structure-based design and synthesis of an antiviral compound (LS5200 SL, DDSyn) 43

Module part: Critical Appraisal of Scientific Papers (LS5200 SP, CritApp) 44

Module part: Tuberculosis (LS5200 TB, Tuberculos) 45

Module part: Virus Host Interaction (LS5200 VHI, VirHostIn) 46

Module part: Cell Biology of the Senses (LS5200 ZB, ZellBioSen) 47

Module part: Experimental Immunology (LS5200 ZD, ExpImmuno) 48

Module part: Advanced course in Immunology (LS5200 ZE, ImmunoFort) 49

Module part: Gene Expression (LS5200 ZF, Genregu) 50

Module part: Cellular Microbiology & Inflammatory Diseases (LS5200 ZK, CellInfDis) 51

Module part: Molecular Biology of Virus Infections of Cythopathogenic and Non-Cytopathogenic Viruses (LS5200 ZM, MolbioVir) 52

Modle part: Inflammatory skin deseases: from bench to bedside (LS5200 ZP, InSkinD) 53

Modle part: Animal experimentation: A comprehensive introduction into laboratory mice (LS5200 ZR, AnimalExp) 54

Module part: Serological and histopathological diagnostic of inflammatory diseases (LS5200 ZS, SeroHist) 55

Module part: Molecular Genetics of inflammatory diseases (LS5200 ZT, MolGenIn) 56

Consolidating in Infection Biology (LS5205, ConsoleIB) 57

Masterthesis Master Thesis Infection Biology (LS5995, MScThesis) 58


Module Guide

LS4015 - Infection Biology 1 (InfBiol1)

Duration: Turnus of offer: Credit points:

1 Semester each winter semester 6

Course of study, specific field and term:● Master Infection Biology (compulsory), Infection Biology, 1st semester

Classes and lectures: Workload:● Infection Biology 1 (lecture, 4 SWS) ● 120 Hours private studies

● 60 Hours in-classroom work

Contents of teaching:● Introduction into the variety of infectious diseases and their respective causative pathogens (Viruses, bacteria, parasitic protozoa and

helminths, fungi).● The lectures will cover human and animal-pathogenic viruses, bacteria and fungi as well as parasites (protozoa, helminths).● Important infectious agents and diseases will be covered in detail such as Influenza, HIV, HCV, herpes viruses, Papilloma, Pox viruses,

Cholera, typhoid fever, E.coli/EHEC, Helicobacter, MRSA, Pneumococci, Tuberculosis, Candida, Malaria, Leishmaniasis, Trypanosoma,Schistosomiasis, Filariasis, Trichinella etc.

Qualification-goals/Competencies:● The students have comprehensive basic and advanced knowledge of the biology of important human infectious agents and their

diseases covering viruses, bacteria, fungi, parasitic protozoa and helminths, their life cycles, vectors and reservoirs, epidemiology,treatment and prophylaxis. The students understand the complex host-pathogen interactions during the infectious process, have thecapacity to integrate the pathogen's virulence functions and the hosts defense strategies, how both evolved during co-evolution andhow these interactions shape pathogenesis and disease outcome. The students have the competence to employ principles ofhost-pathogen interactions in scientific discussions and to use them to approach theoretically and practically research questions.

Grading through:● continuous, successful participation in course● written or oral exam as announced by the examiner

Is requisite for:● Infection Biology 2 (LS4145)

Responsible for this module:● Prof. Dr. Ulrich Schaible

Teacher:● Institute of Virology and Cell Biology● Clinic for Infectiology and Microbiology● Research Center Borstel

● Prof. Dr. Ulrich Schaible● Prof. Ph.D. Tamás Laskay● Prof. Dr. rer. nat. Stefan Taube● Prof. Dr. med. Werner Solbach

Literature:● Sebastian Suerbaum, Helmut Hahn, Gerd-Dieter Burchard, Stefan H. E. Kaufmann: Medizinische Mikrobiologie und Infektiologie - 2012● Richard Goering, Hazel Dockrell, Mark Zuckerman, Ivan Roitt von Saunders: Mims' Medical Microbiology + Student Consult Online

Access - 2012● S.J. Flint et al: Principles of Virology: Molecular Biology, Pathogenesis, and Control of Animal Viruses - American Society Microbiology,

February 2009, 3rd Ed., ISBN: 978-1-55581-443-4● S.Modrow, D. Falke, U. Truyen, H. Schätzl: Molekulare Virologie - Spektrum, Heidelberg, 3. Aufl. 2010, ISBN 978-3-8274-1833-3● Michael T. Madigan, John M. Martinko: Brock Biology of Microorganisms - Pearson International Edition, ISBN 0-13-196893-9● Mims, Nash, Stephen: Mim's Pathogenesis of Infectious Disease - 5th Edition

Language:

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http://www.vuz.uni-luebeck.de/

http://www.hygiene-luebeck.uk-sh.de/Infektiologie_Mikrobiologie/

http://www.fz-borstel.de/

Module Guide

● offered only in English

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Module Guide

LS4035 - Immunology (Immunol)



Course of study, specific field and term:● Master Infection Biology (compulsory), Infection Biology, 1st semester

Classes and lectures: Workload:● Immunology (lecture, 4 SWS) ● 120 Hours private studies


Contents of teaching:● History of Immunology.● Hematopoiesis and hematopoietic stem cells.● Cells of the innate immune system.● Immune sensing by cell-bound pattern recognition systems.● Immune sensing by soluble pattern recognition systems.● B cells, gene rearrangement and antibodies.● MHC, antigen presentation and T cell activation.● T cell subsets, functions and regulation.● Cytokine receptors and signaling.● Soluble mediators and cell trafficking.● Mucosal immunity.● The immune response in allergy.● The immune response in autoimmune diseases.● Introduction: Basic mechanisms of immune defense against pathogens.● The impact of the microbiome on innate and adaptive immune responses.● Immune mechanisms in infection with extracellular bacteria.● Immune mechanisms in infection with intracellular bacteria.● Immune mechanisms in infection with pathogenic protozoa, nematodes, trematodes and cestodes.● Immune mechanisms in infection with DNA and RNA viruses.● The Sepsis syndrome.● Vaccination strategies to prevent infections.● Immune mechanisms related to fungal infections.

Qualification-goals/Competencies:● Understanding the basics in Immunology● Understanding the immune mechanisms in infection● Employing principles of immunology for studies in infection-immunology

Grading through:● continuous, successful participation in course● Viva Voce● written or oral exam as announced by the examiner

Responsible for this module:● Prof. Dr. rer. nat. Marc Ehlers

Teacher:● Research Center Borstel● Institute of Anatomy● Abteilung Molekulare Infektiologie ● Clinic for Infectiology and Microbiology● Institute for Systemic Inflammation Research (ISEF)

● Prof. Dr. rer. nat. Marc Ehlers● Prof. Dr. med. Jörg Köhl● Prof. Dr. rer. nat. Rudolf Manz

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http://www.anat.uni-luebeck.de/

http://www.fz-borstel.de/cms/forschungszentrum/startseite.html


http://www.isef.uni-luebeck.de/

Module Guide

● Prof. Ph.D. Tamás Laskay● Dr. rer. nat. Christoph Hölscher● PD Dr. rer. nat. Norbert Reiling● Dr. rer. nat. Kathrin Kalies● Prof. Dr. med. Jan Rupp● Prof. Dr. med. Werner Solbach● Dr. Christian Karsten● Prof. Dr. med. Jörg Köhl● Dr.rer.nat. Yves Laumonnier● Ph.D. Kensuke Shima● Dr. rer. nat. Inga Kaufhold

Literature:● Kenneth Murphy: Janeway's Immunobiology

Language:● offered only in English

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Module Guide

LS4145 - Infection Biology 2 (InfBiol2)


1 Semester each summer semester 5

Course of study, specific field and term:● Master Infection Biology (compulsory), Infection Biology, 2nd semester

Classes and lectures: Workload:● Infection Biology 2 (lecture, 2 SWS)● Infection Biology 2 (practical course, 3 SWS)

● 75 Hours in-classroom work● 50 Hours private studies

Contents of teaching:● Cellular and molecular mechanisms of infections● Interaction of pathogens with cellular signalling pathways● Extracellular pathogens; biofilms● Intracellular pathogens, molecular mechanisms of intracellular survival, type II-V secretion systems, host cell death and its

consequenses● Immune therapy and DNA vaccines● Immune deficiencies, immunosuppressive chemotherapy and its consequences, retroviruses, HIV-AIDS● Infections and environment● Experimental techniques in the infection biology, in vitro and ex vivo methods, experimental animal models of infectious diseases,

gene knock-out mice, genetically manipulated infectious agents

Qualification-goals/Competencies:● Detailed knowledge of infectious agents, infectious diseases and their pathomechanisms● Detailed understanding of antimicrobial defence mechanisms at the cellular and molecular level.● Understanding the mechanisms of vaccination and immune deficiencies.● Knowledge and practical skills in laboratory techniques of infection biology.

Grading through:● certificates and protocols● continuous, successful participation in course● protocols ● written or oral exam as announced by the examiner

Is requisite for:● Practical Course (LS4115)

Requires:● Diagnosis of Infectious Diseases (LS4045)● Infection Biology 1 (LS4015)

Responsible for this module:● Prof. Ph.D. Tamás Laskay

Teacher:● Institute of Virology and Cell Biology● Research Center Borstel● Clinic for Infectiology and Microbiology

● Prof. Dr. Ulrich Schaible● Prof. Ph.D. Tamás Laskay● Prof. Dr. rer. nat. Marc Ehlers● PD Dr. rer. nat. Norbert Reiling● Prof. Dr. rer. nat. Stefan Taube

Literature:● : - Textbooks, original and review articles

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Module Guide


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Module Guide

LS4175 - Medical Microbiology (MedMicro)



Course of study, specific field and term:● Master Infection Biology (compulsory), Infection Biology, 2nd semester

Classes and lectures: Workload:● See LS4175 A: Molecular Virology (seminar, 2 SWS)● See LS4175 B: Mechanisms of Bacterial Pathogenicity (seminar,

2 SWS)● See LS4175 C: Pathogen Niches (seminar, 2 SWS)● See LS4175 D: Inflammation - Methods in Immunology

(seminar, 2 SWS)

● 120 Hours private studies● 60 Hours in-classroom work

Contents of teaching:● See LS4175 module parts A to D

Qualification-goals/Competencies:● Competence in critical reading, discussing and preseting of research articles.More details see LS4175 module parts A to D

Grading through:● attendance, >90%● presentation● contributions to the discussion

Responsible for this module:● Prof. Dr. rer. nat. Stefan Taube

Teacher:● Institute for Systemic Inflammation Research (ISEF) ● Research Center Borstel● Clinic for Infectiology and Microbiology● Institute of Virology and Cell Biology

● Prof. Dr. rer. nat. Stefan Taube● Prof. Dr. med. Jan Rupp● Prof. Dr. rer. nat. Otto Holst● Dr. rer. nat. Bianca Schneider● Prof. Dr. rer. nat. Marc Ehlers● Prof. Dr. med. Jörg Köhl● Prof. Dr. rer. nat. Rudolf Manz● Prof. Dr. med. Peter König


Notes:

Choice of two of LS 4175 A to D; * graded talk; * min. 4 students/ seminar; If one participate in more than two of the required seminars and talks are given, the two best grades aretaken into account. The additional program achievement will be added non-graded in the transcript of records.

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Module Guide

LS4175 A - Module part: Molecular Virology (MedMicroVi)



Course of study, specific field and term:● Master Infection Biology (module part), Infection Biology, 2nd semester

Classes and lectures: Workload:● Molecular Virology (seminar, 2 SWS) ● 60 Hours private studies


Contents of teaching:● The peer review process● State-of-the art techniques in virology and molecular biology● Recent discoveries of human pathogenic viruses i.e. Influenza, Hepatitis C, Xenotropic murine leukemia virus-related virus (XMRV),

Human Immunodeficiency Virus (HIV), Human Coronavirus (HuCoV-)EMC, Ebola Virus● Data analysis and interpretation● Paper discussion● Paper presentation in a journal club format

Qualification-goals/Competencies:● Can explain the concept of peer review● Can explain principle techniques used in virology and molecular biology● Can give examples of recent developments in human pathogenic viruses● Can interpret an experimental setup and evaluate the correct use of controls● Can evaluate the quality of experimental data● Can present a molecular virology based paper in a journal club format


Responsible for this module:● Siehe Hauptmodul

Teacher:● Institute of Virology and Cell Biology

● Prof. Dr. rer. nat. Stefan Taube


Notes:

* graded talk* min. 4 students/ seminar

(Part of module LS4175)

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Module Guide

LS4175 B - Module part: Mechanisms of Bacterial Pathogenicity (MedMicroBa)




Classes and lectures: Workload:● Mechanisms of Bacterial Pathogenicity (seminar, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Historical background: Milestones in the discovery of the factors involved in microbial virulence● Invasiveness, toxigenesis, colonization, specific adherence● Structure of bacterial cell membrane, cell wall and cell surface: Gram-positive and Gram-negative bacteria, mycobacteria, virulence

factors● Structure and biosynthesis of lipopolysacharides● Structure and biosynthesis of lipoarabinomannan● Structure, biosynthesis and functions of mycobacterial lipids● Recognition of microbial virulence factors by pattern recognition receptors● Recognition of lipids by immune cells, the role of CD1 presentation● Microbial toxins (e.g., leucocidin, hemolysin, botulinum toxin, diphtheria toxin, anthrax toxin, tetanus toxin, pertussis toxin, cholera

enterotoxin, adenylate cyclase, Staphylococcus aureus enterotoxin, TSST, superantigen, shiga toxin, Escherichia coli LT toxin, ST toxin)

Qualification-goals/Competencies:● Can explain structures and biosynthetic pathways of microbial constituents responsible for microbial virulence● Can explain virulence mechanisms of pathogenic microorganisms● Can interpret an experimental setup and evaluate the correct use of controls● Can evaluate the quality of experimental data



Teacher:● Research Center Borstel

● Prof. Dr. rer. nat. Otto Holst● Priv.-Doz. Dr. rer. nat. Sven Müller-Loennies

Literature:● Actual textbooks, original and review articles:


Notes:



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Module Guide

LS4175 C - Module part: Pathogen Niches (MedMicroNi)




Classes and lectures: Workload:● Pathogen Niches (seminar, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Intracellular vs. extracellular pathogens and their nichesBlood vs. tissue, extracellular matrix● Metabolic adaptations of pathogenic microbes and competition with host● Immunopriviledged tissue/organ sitesNiches for immune escape of pathogens● The physiology and cell biology of intracellular microbes● Erythrocytes as host cellsPhagocytes and non-phagocytes as host cells● Microbial biofilms and consortiaNiches formed by immunity and toxins

Qualification-goals/Competencies:● Can explain niches pathogens occupy in the host and how they influence immunity and therapy● Can explain physiological benefits for the pathogens.● Can explain physiological benefits for the pathogens.● Can interpret an experimental setup and evaluate the correct use of controls● Can evaluate the quality of experimental data



Teacher:● Berhard Nocht Institute, Hamburg ● Research Center Borstel

● Dr. rer. nat. Bianca Schneider● Dr.rer.nat. Monica Hagedorn

Literature:● Ulrich E. Schaible, Albert Haas: Intracellular Niches of Microbes: A Pathogens Guide Through the Host Cell - Wiley-VCH 2009● Pascale Cossart, Patrice Boquet, Staffan Normark: Cellular Microbiology - Asm Pr


Notes:



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http://www.bni-hamburg.de


Module Guide

LS4175 D - Module part: Inflammation - Methods in Immunology (MedMicroIn)




Classes and lectures: Workload:● Methods in Immunology (seminar, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Phage display● Flow Cytometry (FACS, MACS, FACS-sort)● Recombinant antibodies● Experimental and therapeutic● Biologica● Conventional, confocal and 2-photon Microscopy● SNPs Analysis● Signaltransduction analysis● Migration Assays● Generation of transgenic, knock-out and knock-in mice● Animal models in Life Science● Microbiome Analysis

Qualification-goals/Competencies:● Can explain principle methods and their applications in immunology● Can give examples of recent developments in immunology● Can interpret an experimental setup and evaluate the correct use of controls● Can evaluate the quality of experimental data● Can present an immunology based paper in a journal club format



Teacher:● Department of Dermatology, Allergology and Venerology● Institute for Systemic Inflammation Research (ISEF)

● Prof. Dr. rer. nat. Marc Ehlers● Prof. Dr. med. Jörg Köhl● Prof. Dr. rer. nat. Rudolf Manz● Prof. Dr. med. Peter König● Dr.rer.nat. Christian Karsten● Prof. Dr. med. Saleh Ibrahim● Dr.rer.nat. Yves Laumonnier


Notes:

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http://www.derma.uni-luebeck.de/


Module Guide



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Module Guide

LS4020 A - Module part: Crystallography (StrAnaKris)



Course of study, specific field and term:● Master Infection Biology (module part), Cellular and molecular Microbiology, 1st semester● Master MML (module part), computational life science / life sciences, 3rd semester● Master MLS (module part), structure biology, 1st semester

Classes and lectures: Workload:● Crystallography (lecture, 2 SWS) ● 60 Hours in-classroom work

● 30 Hours private studies

Contents of teaching:● Crystal growth, precipitant and phase diagram, crystal morphology, symmetry and space groups, crystallogenesis● X-rays, X-ray sources, X-ray diffraction, Bragg's law, reciprocal lattice and Ewald-sphere construction● X-ray diffraction by electrons, Fourier analysis and synthesis● Protein structure determination by X-ray diffraction, crystallographic phase problem, Patterson map, molecular replacement (MR),

multiple isomorphous replacement (MIR), multi-wavelength anomalous diffraction (MAD)● Crystallography and the drug discovery process: studying protein-ligand interactions● Practical exercises at the X-ray generator and at the computer● Site visit at the Synchrotron DESY (Hamburg)

Qualification-goals/Competencies:● Expansion of the basic diffraction theory● Practical exercises in the X-ray laboratory● X-ray analysis in the drug discovery process

Grading through:● written exam


Teacher:● Institute of Biochemistry

● Prof. Dr. rer. nat. Rolf Hilgenfeld● Dr. math. et dis. nat. Jeroen Mesters

Literature:● Jan Drenth: Principles of Protein X-ray Crystallography - Science+Business Media, LLC, New York

Language:● English, except in case of only German-speaking participants

Notes:

This modul is a part of the Modul LS4020-IB und LS4020-MLS

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http://www.biochem.uni-luebeck.de/

Module Guide

LS4020 B - Module part: NMR Spectroscopy (StrAnaNMR)




Classes and lectures: Workload:● NMR-Spectroscopy (lecture, 2 SWS) ● 90 Hours private studies


Contents of teaching:● Lecture topics:● Assignment of NMR spectra● Description of the NOESY experiment using the vector model● Chemical Exchange and Transfer-NOEs● Multidimensional NMR spectroscopy● Assignment strategy for peptides● Introduction into the product operator formalism (POF)● Description of the COSY and of the HSQC experiment using POF● NMR experiments for the assignment of proteins● NMR structural analysis of proteins● Experiments to probe the motions of protein

Qualification-goals/Competencies:● Advanced techniques to assign and analyze NMR spectra● Understanding of NMR experiments based on the product operator formalism● Basic knowledge about NMR experiments to analyze structure and dynamics of proteins



Teacher:● Institute of Chemistry

● Prof. Dr. rer. nat. Thomas Peters● Dr. rer. nat. Karsten Seeger

Literature:● James Keeler: Understanding NMR Spectroscopy - Wiley● Horst Friebolin: Ein- und zweidimensionale NMR-Spektroskopie. Eine Einführung - Wiley-VCH● Malcolm H. Levitt: Spin Dynamics - Basics of Nuclear Magnetic Resonance - Wiley-VCH● D. Neuhaus & M. P. Williamson: The Nuclear Overhauser Effect in Structural and Conformational Analysis - Wiley-VCH● Timothy Claridge: High-Resolution NMR Techniques in Organic Chemistry - Pergamon Press● : Current scientific literature


Notes:

This Modul is a part of Modul LS4020Exercises integrated with lectures

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http://www.chemie.uni-luebeck.de/

Module Guide

LS4020 C - Module part: Single Molecule Methods (Einzelstru)




Classes and lectures: Workload:● Single Molecule Methods (lecture, 2 SWS) ● 60 Hours private studies


Contents of teaching:●

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Qualification-goals/Competencies:●

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Teacher:● Institute of Physics

● Prof. Dr. rer. nat. Christian Hübner

Literature:● Lakowicz, Joseph R: Principles of Fluorescence Spectroscopy - ISBN 978-0-387-46312-4● Markus Sauer, Johan Hofkens, Jörg Enderlein: Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single

Molecules - ISBN: 978-3-527-31669-4


Notes:

This modul is a part of modul LS4020 MLS.It is the same module LS4020 IB-C of Master IB and the same module LS5720T of Master MIW

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http://www.physik.uni-luebeck.de/

Module Guide

LS4020 D - Module part: Microscopy: techniques and applications (StrAnaMikr)




Classes and lectures: Workload:● Microscopy: techniques and applications (lecture, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Light microscopy● Confocal microscopy● 2-photon microscopy● Light sources and detectors● Fluorescent Dyes; GFP and genetically encoded fluorescence markers; Live Cell/tissue imaging: considerations/limitations● Labelling/identifying cell components using fluorescence techniques● Protein-protein Interactions in living cells: FRET, FLIM; Biosensors● Photo-activatable/-switchable Fluorescent Proteins; Fluorescent Timers● Advanced 3D-Fluoresence Microscopy, STED, PALM, STORM● In vivo imaging in tissues and living animals● Applications of Flow Cytometry & Fluorescence-activated Cell Sorting● Electron Microscopy: TEM, Immunogold label; Survey of cell ultrastructure; Correlative EM/light microscopy; Scanning Electron

Microscopy (SEM)● Bioluminescence; high-content screening; outlook: emerging technologies● Data storage/formats; Course discussion; and then: �Cinema of the Cell�

Qualification-goals/Competencies:● Basics of light and fluorescence microscopy and electron microscopy● Detailed knowledge of methods for labelling and visualization of proteins and subcellular compartments● Applications of live cell imaging, in vivo imaging and quantitative fluorescence techniques



Teacher:● Institute for Biology

● Prof. Dr. rer nat. Rainer Duden

Literature:● : http://micro.magnet.fsu.edu/primer/index.html● : http://www.microscopyu.com/smallworld/● : http://www.olympusmicro.com/


Notes:

This module is a part of module LS4020

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http://www.bioweb.uni-luebeck.de/

Module Guide

LS4020 E - Module part: Basic Membrane Biophysics (MemBiophy)



Course of study, specific field and term:● Master Infection Biology (module part), Cellular and molecular Microbiology, 2nd semester

Classes and lectures: Workload:● Basics of Membrane Biophysics (lecture, 2 SWS) ● 45 Hours in-classroom work


Contents of teaching:● Importance and function of cell membranes: structure, physical function and dynamic models● Basics of the membrane components● Thermodynamic self-assembling of lipids and reconstitution techniques● Transmembrane and intrinsic membrane potentials● Mechanical properties of lipid membranes● Physical basics of membrane transport mechanisms● Investigations using lipid monolayer● Electrical and optical experiments using planar lipid bilayers● Examples for interaction mechanisms between peptides/ proteins and planar membranes● Spectroscopic methods on membranes and membrane proteins● Light and force microscopy on membranes and membrane proteins

Qualification-goals/Competencies:● Knowing the constituents and composition of biological membranes● Understanding the physical role and function of membrane lipids and proteins● Knowing the mechanical and electrical properties of membranes● Competence in various methods to investigate reconstituted and natural membranes

Grading through:● written or oral exam as announced by the examiner

Responsible for this module:● Prof. Dr. rer. nat. Christian Hübner


● Prof. Dr. rer. nat. Thomas Gutsmann● PD Dr. rer. nat. Andra Schromm

Literature:● Adam, P. Läuger, G. Stark: Physikalische Chemie und Biophysik - Springer-Verlag, 4. Auflage 2003● W. Hanke, R. Hanke: Methoden der Membranphysiologie - Spektrum Akademischer Verlag, Auflage 1997● O.G. Mouritzen: Life - As a Matter of Fat - Springer ISBN: 987-3-540-23248-3


Notes:

Part of LS4020-IB

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Module Guide

LS4020 F - Module part: Protein-Biophysics (ProBioPhy2)




Classes and lectures: Workload:● Physics of Proteins (lecture, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Protein structure● Energy landscapes● Thermodynamics of protein folding● Kinetics of protein folding● Thermodynamics of enzymatic reactions● Kinetics of enzymatic reactions

Qualification-goals/Competencies:● Understanding of physical principles of:● protein folding● protein dynamics● protein interactions




● Prof. Dr. rer. nat. Christian Hübner● PD Dr. rer. nat. Hauke Paulsen

Literature:● Hans Frauenfelder, Shirley Chan und Winnie Chan: Physics of Proteins: An Introduction to Molecular Biophysics (Biological and Medical

Physics, Biomedical Engineering) - von Springer, Berlin (Gebundene Ausgabe - 30. Dezember 2010)● Alan Fersht: Structure & Mechanism in Protein Science: Guide to Enzyme Catalysis and Protein Folding - W H Freeman & Co

(Gebundene Ausgabe - 15. Februar 1999)


Notes:

Part of LS4020-IB

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Module Guide

LS4020-IB - Structural Biology of Infection (StrucBiol)


1 Semester normally each year in the winter semester 6

Course of study, specific field and term:● Master Infection Biology (compulsory), Cellular and molecular Microbiology, 1st semester

Classes and lectures: Workload:● See LS4020 A: Crystallography (lecture, 2 SWS)● See LS4020 B: NMR Spectroscopy (lecture, 2 SWS)● See LS4020 C: Single Molecule Methods (lecture, 2 SWS)● See LS4020 D: Microscopy, Methods and Application (lecture, 2

SWS)● See LS4020 E: Membrane Biophyics (lecture, 2 SWS)● See LS4020 F: Protein-Biophysics (lecture, 2 SWS)


Contents of teaching:● See Modul parts LS4020 A bis F

Qualification-goals/Competencies:● See Modul parts LS4020 A bis F



Teacher:● Institute for Biology● Research Center Borstel● Institute of Physics● Institute of Biochemistry● Institute of Chemistry

● Prof. Dr. rer. nat. Thomas Peters● Prof. Dr. rer. nat. Rolf Hilgenfeld● Prof. Dr. rer. nat. Christian Hübner● Prof. Dr. rer. nat. Thomas Gutsmann● PD Dr. rer. nat. Andra Schromm● Prof. Dr. rer nat. Rainer Duden● PD Dr. rer. nat. Hauke Paulsen


Notes:

Choice of two courses LS 4020 A-F

If more than two of the required exams passed, the two best grades are taken into account. The additional program achievement will beadded non-graded in the transcript of records.

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Module Guide

LS4045 - Diagnosis of Infectious Diseases (DiagInfDis)



Course of study, specific field and term:● Master Infection Biology (compulsory), Cellular and molecular Microbiology, 1st semester

Classes and lectures: Workload:● Diagnosis of Infectious Diseases (lecture, 2 SWS)● Diagnosis of Infectious Diseases (practical course, 2 SWS)● Diagnosis of Infectious Diseases (seminar, 1 SWS)

● 75 Hours in-classroom work● 50 Hours private studies

Contents of teaching:● Lecture: Infection of the skin and diagnosis; Nucleic acid-based techniques used in the diagnosis of infectious diseases; Diagnosis of

bacterial infectious; Diagnosis of mycobacteria; Serological techniques for the diagnosis of infectious diseases; Diagnosis of viralinfectious; HIV diagnostics; Tropical diseases/ hemorrhagic fever

● Seminar: Selected topics concerning the diagnosis of infectious diseases (i.e. diagnostics of bacterial and viral infections, moleculardiagnostics, resistance testing of bacteria and viruses, biomarkers as novel tools to detect infections, mass spectrometry in clinicalmicrobiology, and application of next generation genome sequencing for infection diagnostics)

● Practical course: Basic laboratory rules and instructions for handling infectious organisms and materials; Techniques of bacteriology:Culture, media, preparation of slides, staining techniques; Characterization and identification of microbes (bacteria, fungi, protozoa,helminiths) by macroscopic and microscopic growth characteristics and morphology; Biochemical characterization of bacteria;Diagnostic immunology/serology: agglutination, precipitation, immunofluorescence; Diagnosis by the novel technique-MALDI-TOF/MS;Analysis of antibiotic susceptibility

Qualification-goals/Competencies:● Students are able to list the different concepts for the diagnosis of infectious diseases (pathogenic bacteria, fungi, virus, protozoa and

helminits). They are able to illustrate and discuss these concepts with the aid of appropriate examples. They are able to assess thepotential and the limitation of a given diagnostic concept and to propose alternative strategies. They do understand and are able toexplain the underlying principles of a given technique. They are able to identify unknown pathogens from suspected infectiousmaterials of skin, respiratory, intestinal, urinary tract and blood infections by various diagnostic techniques. They acquire competencesin presenting and discussing scientific results

Grading through:● continuous, successful participation in practical course● presentation● protocols ● written or oral exam as announced by the examiner

Is requisite for:● Infection Biology 2 (LS4145)

Responsible for this module:● Prof. Dr. med. Werner Solbach

Teacher:● LADR GmbH Geesthacht● Institute for Systemic Inflammation Research (ISEF) ● Berhard Nocht Institute, Hamburg ● Department of Dermatology, Allergology and Venerology● Research Center Borstel● Institute of Molecular Medicine● Clinic for Infectiology and Microbiology

● Prof. Dr. med. Werner Solbach● Prof. Dr. med. Johannes Knobloch● Prof. Dr. rer. nat. Tobias Restle● Prof. Dr. rer. nat. Stefan Niemann

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Module Guide

● Dr. rer. nat. Alessandra Mescalchin● Prof. Dr. rer. nat. Georg Sczakiel● Dr. rer. nat. Martina Behnen-Haerer● Ph.D. Kensuke Shima● Prof. Dr. rer. nat. Stefan Taube● Prof. Dr. rer. nat. Norbert Tautz● Prof. Dr. rer. nat. Marc Ehlers● Prof. Dr. Stephan Günther● PD Dr. rer. nat. Elvira Richter● Dr. med. Waltraud Anemüller● PD Dr. med. Jan Kramer


Notes:

The module �diagnosis of infectious diseases� is required for the participation to �Infection Biology 2�.

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Module Guide

LS4155 - Anti-Microbial Therapy and Prophylaxis (AntTherPro)



Course of study, specific field and term:● Master Infection Biology (compulsory), Cellular and molecular Microbiology, 2nd semester

Classes and lectures: Workload:● Anti-Microbial Therapies (lecture, 2 SWS)● Vaccination Strategies (seminar, 2 SWS)


Contents of teaching:● General concepts of anti-microbial therapies; potentials and limitations, Concepts in drug design, Alternative strategies, The problem of

drug resistance, Future challenges,● Selected examples: antibacterial (antibiotics), antiviral (e.g. polymerase and protease inhibitors of HIV, Herpes), antifungal (e.g.

antimycotics and Candida albicans), antiprotozoal (e.g. chloroquine and Malaria) and drugs against multicellular eukaryotes (e.g.anthelmintics and fox-tapeworm).

● Vaccination strategies:Pathogen niches and immunity, Determine vaccine types, Vaccine types (live attenuated, killed, subunit,recombinant live and examples), Epitopes, Vaccine carriers, Adjuvants

Qualification-goals/Competencies:● Students are able to list the different concepts of antimicrobial therapies (directed against: bacteria, viruses, fungi, worms and

protozoa) and are able illustrate these therapies with the aid of appropriate examples. They are able to assess the potential and thelimitation of a given therapy concept and to propose alternative strategies. They are able to list and discuss the general concepts ofanti-microbial prophylaxis (i.e. vaccination). They acquire competences in presenting and discussing scientific results.

Grading through:● written exam● Marked presentation

Responsible for this module:● Prof. Dr. rer. nat. Tobias Restle

Teacher:● Institute of Biochemistry● Research Center Borstel● Institute of Molecular Medicine

● Prof. Dr. rer. nat. Tobias Restle● Prof. Dr. rer. nat. Rolf Hilgenfeld● Prof. Dr. Ulrich Schaible● Dr. med. Andreas Paech

Literature:● : Recent review articles


Notes:

Graded exam and talk (50/50).

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Module Guide

LS4185 - Host-Pathogen Interaction (HostPatInt)



Course of study, specific field and term:● Master Infection Biology (compulsory), Cellular and molecular Microbiology, 2nd semester

Classes and lectures: Workload:● See LS4185 A: Analysis of Host-Pathogen Interaction (lecture

and practical course, 2 SWS)● See LS4185 B: Rational Drug Design (lecture, 2 SWS)


Contents of teaching:● See LS4185 module parts A or B

Qualification-goals/Competencies:● More details see LS4185 module parts A or B


Responsible for this module:● Prof. Dr. med. Peter König

Teacher:● Institute of Biochemistry● Ernst-Moritz-Arndt-University of Greifswald● Berhard Nocht Institute, Hamburg ● Research Center Borstel● Institute of Chemistry● Institute of Anatomy


Notes:

Choice of one course of LS4185A or B

* more details see LS4185A or B

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Module Guide

LS4185 A - Module part: Analysis of Host Pathogen Interaction (AnalHPI)




Classes and lectures: Workload:● Analysis of Host Pathogen Interaction (lecture and practical

course, 2 SWS)● 60 Hours private studies● 30 Hours in-classroom work

Contents of teaching:● Introduction to host-pathogen interaction using different examples● Importance of commensal colonization● Introduction to methods used for the analysis of host-pathogen interaction with focus on omics methods to analyze genes, proteins

and lipids and imaging methods to visualize host-pathogen interaction

Qualification-goals/Competencies:● Understanding of different approaches to investigate how host and pathogen can interact and its impact on disease● Knowledge of in vivo and in vitro methods to analyze pathogen-host interaction● Insight into experimental design and data analysis of imaging and omics experiments

Grading through:● presentation● written exam


Teacher:● Berhard Nocht Institute, Hamburg ● Ernst-Moritz-Arndt-University of Greifswald● Institute of Anatomy● Research Center Borstel

● Prof. Dr. med. Peter König● Prof. Dr. Ulrich Schaible● Dr. Frank Schmidt● Dr.rer.nat. Monica Hagedorn● Prof. Dr. rer. nat. Stefan Niemann● Dr. Dominik Schwudke● Dr. Silke Feuerriegel ● Dr. Susanne Homolka


Notes:

(Is part of LS4185)

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Module Guide

LS4185 B - Module part: Rational Drug Design (RatDruDes)




Classes and lectures: Workload:● Rational Drug Design (lecture, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Drug development � an overview● Target identification and validation● Role of x-ray crystallography in drug development● Structure-based drug design � Principles and methods● Case studies of structure-based drug development● Combinatorial approach for nucleic acid therapeutics identification● Oligomeric nucleic acid therapeutics● Cellular applications of nucleic acid therapeutics via non-viral carrier systems

Qualification-goals/Competencies:● Basic strategies of drug design● The path from the discovery of an active principle to a marketable product. Rational drug design● NMR and crystallography as fundamental tools for finding and optimizing active agents● Structure and effect relationships will be demonstrated using examples, techniques used for theoretical prediction and experimental

proof will be introduced, especially the complementary approach using crystallographic methods and NMR experiments● Students will critically evaluate these methods and perceive their limits



Teacher:● Institute of Biochemistry● Institute of Chemistry

● Prof. Dr. rer. nat. Thomas Peters● Prof. Dr. rer. nat. Rolf Hilgenfeld● Dr. rer. nat. Alessandra Mescalchin● Prof. Dr. rer. nat. Tobias Restle● PD Dr. phil. nat. Thomas Weimar● Dr. phil. nat. Hannelore Peters


Notes:

(Part of module LS4185

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Module Guide

CS1400 T - Module part: Introduction to Bioinformatics (Bioinfo)



Course of study, specific field and term:● Master Infection Biology (module part), Competencies and clinical aspects, 1st semester

Classes and lectures: Workload:● Introduction to bioinformatics (lecture, 2 SWS)● Introduction to bioinformatics (exercise, 1 SWS)

● 55 Hours private studies● 45 Hours in-classroom work● 20 Hours exam preparation

Contents of teaching:● Life, Evolution & the Genome● Sequence assembly - Industrial reading of genetic information● DNA sequence models & hidden markov models● Viterbi-Algoritm● Sequence alignment & dynamic programming● Unsupervised data analysis (k-means, PCA, ICA)● DNA microarrays & GeneChip technologies

Qualification-goals/Competencies:● Students are able to explain the basic concepts of coding, transcription and translation of information in living beings.● They are able to explain how a solution of the shortest common superstring problem can be estimated with a simple greedy algorithm.● They are able to create a Markov chain or a Hidden Markov Model (HMM) for a given modelling problem.● They are able to give examples on how to solve a problem using dynamic programming.● They are able to implement the introduced algorithms (in Matlab)● They are able to use unsupervised learning methods and they are able to interpret the results.● They are able to explain basic Microarray-and DNA-Chip-Technologies.

Grading through:● exercises● written or oral exam as announced by the examiner

Responsible for this module:● Prof. Dr. rer. nat. Amir Madany Mamlouk

Teacher:● Institute for Neuro- and Bioinformatics

● Prof. Dr. rer. nat. Amir Madany Mamlouk

Literature:● H. Lodish, A. Berk, S. L. Zipursky and J. Darnell: Molekulare Zellbiologie - Spektrum Akademischer Verlag, 4. Auflage, 2001, ISBN-13:

978-3827410771● A. M. Lesk: Introduction to Bioinformatics - Oxford University Press, 3. Auflage, 2008, ISBN-13: 978-0199208043● R. Merkl and S. Waack: Bioinformatik Interaktiv: Grundlagen, Algorithmen, Anwendungen - Wiley-VCH Verlag, 2. Auflage, 2009, ISBN-13:

978-3527325948● M. S. Waterman: Introduction to Computational Biology - Chapman and Hall, 1995

Language:● offered only in German

Notes:

Part of module CS4011.

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Module Guide

CS4011 - Bioinformatics, Modelling and Biostatistics (BioModEpi)



Course of study, specific field and term:● Master Infection Biology (compulsory), Competencies and clinical aspects, 1st semester

Classes and lectures: Workload:● See CS1400 T: Introduction to Bioinformatics (course, 3 SWS)● See MA3200 T: Genetic Epidemiology (course, 3 SWS)

● 120 Hours (see module parts)

Contents of teaching:● See possible module parts

Qualification-goals/Competencies:● See possible module parts

Grading through:● exercises● written or oral exam as announced by the examiner

Responsible for this module:● Prof. Dr. rer. nat. Thomas Martinetz

Teacher:● Institute of Medical Biometry and Statistics● Institute for Neuro- and Bioinformatics

● Prof. Dr. rer. nat. Thomas Martinetz● Prof. Dr. rer. nat. Andreas Ziegler● Prof. Dr. rer. biol. hum. Inke König

Language:● depends on the chosen courses

Notes:

You must choose exactly one of the module parts CS1400 T or MA3200 T.

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Module Guide

LS4025 - Clinical Aspects of Infection (ClinAsp)



Course of study, specific field and term:● Master Infection Biology (compulsory), Competencies and clinical aspects, 1st semester

Classes and lectures: Workload:● Lectures clinical aspects of infection (lecture, 3 SWS)● Excercises clinical aspects of infection (exercise, 1 SWS)


Contents of teaching:● Clinical background: Clinical characteristics and diagnostical approaches of the most important infectious diseases. Pathophysiological

background on the occurence of systemic and local clinical symptoms and disease-specific clinical pictures. Introduction in the mostrelevant instrumental and laboratory techniques for the diagnosis of infections. Established and novel strategies in the treatment ofinfectious diseases with respect to the emergence of multi-drug resistant pathogens.

● Modern therapeutic approaches● Clinical epidemiology: Introduction into epidemiology; What is normal? Diagnosis; Frequencies; Registers and data sources;

Geographical epidemiology; Study designs (RCT, cohort study, case control study, cross sectional study); Effect measures; Causality;Chance, bias and confounding; Control of errors.

● Assessment of scientific articles on the basis of clinical epidemiology●

●

Qualification-goals/Competencies:● Basic knowledge on clinical aspects of infectious diseases● Students are able to explain technical terms such as disease register, incidence, prevalence, mortality, lethality.● Students are able to explain and to interprete epidemiological measures.● Students are able explain which study design is appropiate for which specific study question.● Students are able to judge if the methods that were used in a particular study will result in valid or biased results.● Students are able to formally and critically appraise the internal and external validty and the reporting quality of a scientific paper by

means of checklists.● Soft Skills:By means of small group discussions students' communication competencys and capacity to team work should be increased.● Understanding the links between clinic - infection medicine for diagnosis, patient management and therapyBasic knowledge on clinical

aspects of infectious diseases●

Grading through:● continuous participation in all courses of the module● written or oral exam as announced by the examiner

Responsible for this module:● Prof. Dr. med. Jan Rupp

Teacher:● Medical Clinic I● Clinic for Infectiology and Microbiology● Research Center Borstel● Institute for Social Medicine and Epidemiology

● Prof. Dr. med. Jan Rupp● Prof. Dr. med. Alexander Katalinic● Prof. Dr. med. Johannes Knobloch● Prof. Dr. Christoph Lange● PD Dr. rer. nat. Annika Waldmann● Dipl.-Inf. Heiner Fauteck● Dipl.-Stat. Nora Eisemann● Dr. med. Gunar Günther

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Module Guide

● Dr. med. Claudia Jafari● Prof. Dr. med. Christian Sina● Dr. med. Barbara Kalsdorf● Dr. med. Jochen Krajewski● Dr. med. Jan Heyckendorf● Dr. med. Maria Raili Noftz

Literature:● Mims, Nash, Stephen: Mims' Pathogenesis of Infectious Disease - 5th edition● Fletcher. Fletcher & Fletcher: Clincal Epidemiology. The Essentials. 5th edition


Notes:

You are only allowed 2 apoligized missed days.

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Module Guide

LS4165 - Model Systems of Infection (ModSysInf)



Course of study, specific field and term:● Master Infection Biology (compulsory), Competencies and clinical aspects, 2nd semester

Classes and lectures: Workload:● Lectures In vivo Models (lecture, 3 SWS)● Excercises In vivo Models (exercise, 2 SWS)● Practical course In vivo Models (practical course, 2 SWS)


Contents of teaching:● (1) Animal experimentation and biological safety; Lecture: (a) animal protection laws, animal experimentation application,

documentationof animal experiments, anatomy and physiology of the mouse, breeding methods and nomenclature, transgenic mice,anesthetics,analgesia, methods of anesthesia, criteria for animal burden and experiment abortion, ethics, alternative and additionalmethods,biological and gene technological safety. (b) Practicals: biology and handling of mouse, handling and behaviour of mouse,health and gender controling, applicationmethods, blood sampling and animal protection appropriate sacrifice, sectioning mouse,working under biological safety levels,transgenic technology

● (2) Clinic-near model systems in infection biology: principles of animal experimentation in infection biology, infections of theskin(leishmaniasis), lung (tuberculosis, influenza), intestinal (helminths, salmonella), intracerebral (toxoplasmosis) and systemicinfections(trypanosomis, malaria, sepsis), humanized animal experimental models, comparison of scientific results from animalexperimentationand the situation in humans

Qualification-goals/Competencies:● Basic knowledge of laws regulating animal experimentaion; theoretical and practical basics in handling of experimental animals;

basicknowledge on aspects of biological and gene technological safety; basic knowledge on the generation of transgenic animals● Basic knowledge based on experimental examples; consolidation of knowledge in seminar● Knowing how to handle the model systems; Competence in protocol writing


Responsible for this module:● Dr. rer. nat. Christoph Hölscher

Teacher:● Institute for Systemic Inflammation Research (ISEF) ● Clinic for Infectiology and Microbiology● Research Center Borstel

● Dr. rer. nat. Christoph Hölscher● Prof. Ph.D. Tamás Laskay● Prof. Dr. rer. nat. Stefan Taube● Dr. rer.nat. Hanna Erdmann● PD Dr. rer. nat. Norbert Reiling● Dr. rer. nat. Bianca Schneider● Prof. Dr. Guntram Grassl Grassl● Dr. Kerstin Walter● Dr.rer.nat. Christian Karsten● Dr. rer. nat. Jochen Behrends


Notes:

*attendance in lecture and practical course min. 90%

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Module Guide

MA3200 T - Module part: Genetic Epidemiology (GenEpi)


1 Semester every second year 4

Course of study, specific field and term:● Master Infection Biology (module part), Competencies and clinical aspects, 1st semester

Classes and lectures: Workload:● Genetic Epidemiology (lecture, 2 SWS)● Genetic Epidemiology (exercise, 1 SWS)

● 60 Hours private studies● 45 Hours in-classroom work● 15 Hours exam preparation

Contents of teaching:● Fundamentals in molecular genetics: Genetic information, transmission and variation of genetic information, genotyping methods● Fundamentals in formal genetics: Mendelian laws, segregation patterns, Hardy-Weinberg-equilibrium● Genetic markers● Data quality: Errors in the data, methods of error detection● Association studies: Study designs, tests, estimates, linkage disequilibrium, bias in the data● Haplotype-based association: Estimation of haplotypes, tests, haplotype blocks● Genome-wide association: Study designs, study conduct, specific problems● Family-based association: Transmission-disequilibrium and other tests, estimates, sample size estimation

Qualification-goals/Competencies:● Students are able to describe the generation of genetic data, its error sources and methods of detection.● They can select and describe the most important approaches for genetic epidemiological association studies on the level of single

markers and haplotypes.● They are able to apply the basic test procedures manually and more complex test procedures using the computer and to interpret the

results.● They have the methodological competence to solve large-scale tasks cost- and time- efficiently.● They have the management competence to organize their own work and that of collaborators involved in the project.● They have the methods competence to develop solutions with limited resources (time, personnel, etc.) that comply with general

quality criteria.


Responsible for this module:● Prof. Dr. rer. biol. hum. Inke König

Teacher:● Institute of Medical Biometry and Statistics

● Prof. Dr. rer. biol. hum. Inke König● MitarbeiterInnen des Instituts

Literature:● Ziegler A, König IR.: A statistical approach to genetic epidemiology. Concepts and applications. - 2010. ISBN: 978-3-527-32389-0


Notes:

Is the same as MA3200Is module part of CS4011

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Module Guide

PS4610 - Ethics in Sciences / Scientific Writing (EthScWr)


1 Semester each summer semester 7 (Typ B)

Course of study, specific field and term:● Master MLS (compulsory), interdisciplinary competence, 4th semester● Master Infection Biology (compulsory), Competencies and clinical aspects, 4th semester

Classes and lectures: Workload:● Ethics in Sciences (seminar and project work, 2 SWS)● Scientific Writing (seminar and project work, 2 SWS)


Contents of teaching:● See module parts

Qualification-goals/Competencies:● See module parts

Grading through:● Oral presentation and written report● continuous participation (>80%)● written or oral exam as announced by the examiner● Marked presentation with written report

Responsible for this module:● Prof. Dr. rer. nat. Georg Sczakiel

Teacher:● Institute for the History of Medicine and Science Studies● Institute of Molecular Medicine

● Prof. Dr. rer. nat. Georg Sczakiel● Prof. Dr. phil. Christoph Rehmann-Sutter


Notes:

The module is successfully graduated if both courses are passed. The certificate will be ungraded (B-certificate).

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Module Guide

PS4610 A - Module part: Ethics in Sciences (Ethics)


1 Semester each summer semester 3,5

Course of study, specific field and term:● Master MLS (module part), interdisciplinary competence, 4th semester● Master Infection Biology (module part), Competencies and clinical aspects, 4th semester

Classes and lectures: Workload:● Ethics in Sciences (lecture, 2 SWS) ● 40 Hours private studies

● 30 Hours in-classroom work● 20 Hours exam preparation

Contents of teaching:● Societal and ethical implications of research in biomedical sciences and technologies● Basics of philosophy and sociology of science● Good scientific practice● Basics of bioethics: duties of investigators, obligations to colleagues,● Ethics of human subjects research and animal experim. Environmental ethicsentation. Control and governance of technology. Risk

assessement● Use and implications of images in science

Qualification-goals/Competencies:● You can explain the methodology of the physical sciences and their philosophical basis● You can recognize ethical dimensions of practice and deciding● You can understand relevant laws in Germany● You can participate in current discussions in bioethics and research ethics● You can reflect on ethical dimensions of biomedical sciences● You can write a structured ethics paper about a self-chosen topic

Grading through:● Marked presentation with written report


Teacher:● Institute for the History of Medicine and Science Studies

● Prof. Dr. phil. Christoph Rehmann-Sutter

Literature:● Daniel A. Vallero: Biomedical Ethics for Engineers. Ethics and Decision Making in Biomedical and Biosystem Engineering - Amsterdam:

Elsevier 2007● Ben Mepham: Bioethics. An Introduction for the Biosciences - Oxford: Oxford University Press 2008● Sergio Sismondo: An introduction to science and technology studies - Chichester: Wiley-Blackwell 2010


Notes:

Part of PS4610.

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Module Guide

PS4610 B - Module part: Scientific Writing (SciWrit)


1 Semester each winter semester 3,5

Course of study, specific field and term:● Master MLS (module part), interdisciplinary competence, 4th semester● Master Infection Biology (module part), Competencies and clinical aspects, 4th semester

Classes and lectures: Workload:● Scientific Writing (seminar, 2 SWS) ● 120 Hours private studies


Contents of teaching:● Basics of ethics and moral philosophy● The ethical structure of experiments with tissue, animals and human subjects● Principles of the most important laws and guidelines regulating research● Basic issues of research ethics and cases from recent debates● Key topics of research ethics in the biomedical sciences● Introduction into categories of scientific presentations● Analysis of scientific manuscripts and rules for their presentation● Preparation and presentation of scientific posters● Preparing a project proposal

Qualification-goals/Competencies:● Understanding of basic ethical dimensions of human actions and decisions● Understanding of ethical implication of experimental scientific research● Knowledge of relevant legal regulations in Germany and internationally● Knowledge of key debates in bioethics and research ethics● Basic skills for an autonomous ethical reflection about issues in biomedical sciences● Analysis of the logical and formal structure of scientific publications. Analysis of a specific original publication. Introduction into the

´peer-review process´● Understanding the criteria underlying scientific posters. Preparation and presentation of a poster based on given experimental data● Introduction into the writing of ´grant applications´ and the funding process of research projects. Writing a grant application on the

basis of specified prior-work and scientific aims

Grading through:● term paper● continuous, successful participation in course● written or oral exam as announced by the examiner


Teacher:● Institute of Molecular Medicine

● Prof. Dr. rer. nat. Georg Sczakiel

Languages:● offered only in English● English, except in case of only German-speaking participants

Notes:

Part of PS4610.

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Module Guide

LS4115 - Practical Course (PC)



Course of study, specific field and term:● Master Infection Biology (compulsory), Interdisciplinary internships and consolidation courses, 3rd semester

Classes and lectures: Workload:● Practical Courses (block practical course, 24 SWS) ● 360 Hours in-classroom work


Contents of teaching:● In two practical courses in each case 2 of the different skills, described below, have to be acquired. At least one practical course must

have a duration of 3 months (12 weeks) full time. The second one can last 8 to 12 weeks.● Molecular microbiology:M 1: Genomics/ transcriptomicsM 2: Proteomics/ lipidomicsM 3: Structure analytics of macromoleculesM 4:

Protein expression and -purificationM 5: Membrane biophysicsM 6: RNA/ siRNA-TechnologiesM 7: Molecular Genetics ofmicrobes/pathogens

● Cellular microbiology: C 1: Tissue culture/ Cell cultureC 2: Microbe-infected cell/ tissue modelsC 3: In vivo infection modelsC 4: Celltracing/ Cell sorting/ Cellular biochemistryC 5: ImmunologyC 6: Microscopic Techniques/ Ultrastructure

● Medical Microbiology Me1: Diagnostic tools in microbiology and serologyMe2: Tropical medicineMe3: Infection-epidemiology

Qualification-goals/Competencies:● Extension and application of the theoretically gained experience to the experimental work in two fields of Cellular- and Molecular

microbiology (called competences) in each course;absorbing knowledge in documentation and presentation of scientific data(presentation with defense)ability to work in a team;getting lab experiences by working on real research projects

● Live long learning competence

Grading through:● continuous, successful participation in practical course● presentation● Poster

Is requisite for:● Master Thesis Infection Biology (LS5995)

Requires:● Diagnosis of Infectious Diseases (LS4045)● Infection Biology 2 (LS4145)● Infection Biology 1 (LS4015)


Teacher:● All institutes of the University of Lübeck

● MitarbeiterInnen des Instituts


Notes:

All practical courses must be approved by Prof. Schaible 4 weeks in time before.

Two internships musst be passed during totaly 22 weeks. The Results will be presented by poster or talk. The presentation will be graded.

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Module Guide

LS5200 PM - Module part: Project Management (ProjectM)



Course of study, specific field and term:● Master MLS (module part), advanced curriculum MLS, 3rd semester● Master Infection Biology (module part), Interdisciplinary internships and consolidation courses, 3rd semester

Classes and lectures: Workload:● Project Management (seminar / practical course / exercise, 2

SWS)● 60 Hours private studies● 30 Hours in-classroom work

Contents of teaching:● This introductory lecture provides a comprehensive account of project management, i.e. the application of knowledge, skills and

techniques to execute projects effectively and efficiently. Project management is a strategic competency for individuals andorganisations alike, enabling them to relate project results to business goals and thus improve their performance in their markets.

● Introduction to Project Management● Project Management as a Business Process● Starting and Planning Projects● Cross Cultural Collaboration● Risk Management● Quality Management● Implementing and Controlling Projects● Leading Projects● Completing Projects● Different areas of knowledge and process groups will be covered over the course of the project life cycle. Lectures, presentations and

team-oriented practices will lead to students familiarising themselves with the terminology, the tools and the techniques used inproject management.

Qualification-goals/Competencies:● Students can single-handedly apply knowledge, skills and techniques in order to effectively and efficiently execute projects (such as

master and PhD theses, among others). They will also gain a thorough understanding of relevant differences between different levelsof project management, the importance of cultural dimensions and success factors to be considered in projects, methods of dealingand coping with certain peculiarities in projects as well as a guide to conduct projects. Students will further be able to do project andteam work, lead projects and argue with regards to their project solutions in a constructive manner.

Grading through:● presentation


Teacher:● Institute of Virology and Cell Biology● University of Applied Sciences

● Prof. Dr. Leef Dierks


Notes:

For MLS and IB.Requirements: All modules of the 1st and 2nd semesters MSc Infection Biology must be passed.

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Module Guide

LS5200 SA - Modul part: Lipidomics and biological mass spectrometry (Massenspek)



Course of study, specific field and term:● Master Infection Biology (module part), Interdisciplinary internships and consolidation courses, 3rd semester● Master MLS (module part), advanced curriculum MLS, 3rd semester

Classes and lectures: Workload:● Lipidomics and biological mass spectrometry (seminar with

practical exercises, 2 SWS)● 60 Hours private studies● 30 Hours in-classroom work

Contents of teaching:● Analytical chemistry● sample preparation techniques● mass spectrometric principles● proteomics● lipidomics● LC-MSn based quantification

Qualification-goals/Competencies:● Understanding mass spectrometric Principles and its application in life sciences




● Dr. Dominik Schwudke

Literature:● : Mass spectrometry-based proteomics - Nature 2003 / doi:10.1038/nature01511● : Shotgun lipidomics on high resolution mass spectrometers - Cold Spring Harb Perspect Biol. 2011 / doi: 10.1101/cshperspect.a004614.

Language:● German and English skills required

Notes:

Prerequisites: Passed all modules of the 1. and 2. semester MSc.One week in March

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Module Guide

LS5200 SB - Module part: Biochemistry of the bacterial envelope (BacEnv)




Classes and lectures: Workload:● Biochemistry of the bacterial envelope (lecture with exercises

or seminar, 2 SWS)● 60 Hours private studies● 30 Hours in-classroom work

Contents of teaching:● Part 1: Glycolipids, lipoproteins, membrane components: Introduction● Structure, occurrence and properties● Synthesis, biosynthesis and decomposition● Structural analysis of lipids and lipoconjugates● Application and impact of lipoconjugates in bio-medicine: function● Lipoconjugates in Immunology● Pathobiochemistry of lipids (functional disorders)● Signal transduction in membranes● Part 2 Oligo- and polysaccharides and glycoproteins:Introduction● Structure, occurrence and properties● Synthesis, biosynthesis and decomposition● Structural analysis of polysaccharides and glycoproteins● Function: Impact and application of polysaccharides and glycoproteins● Pathobiochemistry

Qualification-goals/Competencies:● Understanding the structure-function ratio of biologically and biomedically important molecules● Understanding problems of modern bioinformatics● Knowledge of structures of molecules

Grading through:● presentation● written or oral exam as announced by the examiner● written exam



● Prof. Dr. rer. nat. Ulrich Zähringer● Prof. Dr. rer. nat. Otto Holst

Literature:● F. Lottspeich und H. Zorbas (Hrsg.).: Bioanalytik - Spektrum, Akademischer Verlag, Heidelberg, Berlin, 1. Auflage 1998● Lehmann, Jochen: Kohlenhydrate: Chemie und Biologie - Wiley-VCH, 1996, ISBN 3-527-30859-8


Notes:


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Module Guide

LS5200 SD - Module part: Molecular Dynamics (MolDyn)




Classes and lectures: Workload:● Molecular Dynamics (lecture, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Energy minimization and discrete Newtonian dynamics (Verlet algorithm)● Force fields: stretching, bending, torsion, fields of application (proteins, DNA, universal)● Treatment of Coulomb and van der Waals interactions (cutoffs, Ewald summation)● Influence of temperature and pressure: thermostats and barostats● Hybrid methods: classical force fields combined with quantum mechanical treatment● Examples using gromacs (www.gromacs.org)

Qualification-goals/Competencies:● You are able to list the interactions between  atoms and molecules● You can explain in which framework these  interactions can be described by classical models● You know different types of molecular dynamics  simulations and can explain for which applications  they are useful

Grading through:● Recitation● written exam



● PD Dr. rer. nat. Hauke Paulsen

Literature:● A. Leach: Molecular modelling


Notes:

For MLS and IB.Prerequisites: Passed all modules of the 1. and 2. semester MSc

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Module Guide

LS5200 SF - Module part: Structural Aspects of Protein biosynthesis (ProtBSynth)




Classes and lectures: Workload:● Structural Aspects of Protein biosynthesis (seminar, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Seminar series● Analysis of the most recent and relevant scientific publications and presentation thereof followed by a discussion

Qualification-goals/Competencies:● Students are able to present and explain the theoretical foundation of a central biological process called protein biosynthesis● They have learned to screen, condense and present information on a particular topic● They have improved their analytical competences● They have learned to scrutinize, compare and judge the value of written information● They have improved their communication competence

Grading through:● presentation in English● participation in discussions



● Dr. math. et dis. nat. Jeroen Mesters

Literature:● Anders Liljas: Structural Aspects of Protein Synthesis - World Scientific Publishing, London Singapore, ISBN 981-238-867-2● : Current research and review articles


Notes:


40



Module Guide

LS5200 SH - Module part: Light into Darkness: Modern Fluorescence Techniques in Structural Biology (ModernF)

Duration: Turnus of offer: Credit points: Max. group size:

1 Semester each winter semester 3 4


Classes and lectures: Workload:● Light up the Dark: modern fluorescence techniques in

structural biology (lecture, 2 SWS)● 60 Hours private studies● 30 Hours in-classroom work

Contents of teaching:● Basics in fluorescence● Photo physics● Energy transfer● Single molecule fluorescence● Fluorescence correlation spectroscopy

Qualification-goals/Competencies:● You understand modern fluorescence methods● You gain expertise in quantitative data analysis● You achieve ability to work with current literature● You are enabled for the assessment of scientific results●

Grading through:● Recitation● written report



● Prof. Dr. rer. nat. Christian Hübner

Literature:● : Current publications


Notes:


41



Module Guide

LS5200 SI - Module part: Characterization of Protein Complexes in Structural Biology (ProtCompl)




Classes and lectures: Workload:● Characterization of Protein Complexes in Structural Biology

(Seminar and practical course, 2 SWS)● 60 Hours private studies● 30 Hours in-classroom work

Contents of teaching:● Protein complex formation as the basis of all biochemical cascade● Basic techniques to study protein complexes. Theories to be covered: Fluorescence Resonance Transfer (FRET)/ Isothermal Calorimetry

(ITC)/ Surface Plasmon Resonance (SPR)/ Circular Dichroism (CD)● Experimental techniques to be covered: Protein Complex Preparation/ FRET and SPR

Qualification-goals/Competencies:● This module aims to address at expanding the knowledge of biophysical and biochemical characterization methods of protein

complexes, which is the basis of all working enzymatic cascades. The module will include lectures and hands-on practical sessions. Wewill also visit CSSB/DESY

● You will learn:● How to prepare a protein complex● How to label proteins with fluorescent dye for single molecule fluorescence measurements● UV / Vis spectra and discussions thereof● How to setup the experiments to determine dissociation constant of the complex formation● Different method for determination of complex formation (FRET, SPR)● Discussion of the obtained results and comparisons of the results with other available structural data.●

Grading through:● attendance, >90%● presentation


Teacher:● Institute of Virology and Cell Biology● Institute of Physics

● Dr. Young-Hwa Song

Literature:● : A few recent review articles will be provided.


Notes:


42




Module Guide

LS5200 SL - Module part: Structure-based design and synthesis of an antiviral compound (DDSyn)




Classes and lectures: Workload:● Structure-based design and synthesis of an antiviral compound

(seminar, 2 SWS)● 60 Hours private studies● 30 Hours in-classroom work

Contents of teaching:● Experiment 1: Recombinant production of 3C-protease (3Cpro) of Coxsackievirus B3 in E. coli, purification of protein, activity assays● Experiment 2: Crystallization of 3Cpro. Conditions are chosen allowing for crystal growth in only few hours● Experiment 3: Collection of diffraction data of these crystals or, in case of not sufficient quality, of primed crystals● Experiment 4: Interpretation of electron densitiy and modelling of an inhibitor● Experiment 5: Practical performance of two steps of the synthesis of an inhibitor, determination of the identity of the product via ESI

mass spectrometry and NMR spectroscopy● Experiment 6: Determination of kinetic parameters using FRET: IC50 and kkat/KM● Experiment 7: Working with replicons, transfection, determination of EC50 - values

Qualification-goals/Competencies:● This practical course offers an interdisciplinary insight in design and chemical synthesis of antiviral compounds. All practical work is

performed by the students themselves. The course is based on the knowledge of the following courses: virology, biochemistry,structure analysis/crystallography, rational drug design, organic chemistry, biological chemistry

Grading through:● protocols



● Prof. Dr. rer. nat. Rolf Hilgenfeld● MitarbeiterInnen des Instituts

Language:● offered only in German

Notes:


43



Module Guide

LS5200 SP - Module part: Critical Appraisal of Scientific Papers (CritApp)



Course of study, specific field and term:● Master Infection Biology (module part), Interdisciplinary internships and consolidation courses, 3rd semester

Classes and lectures: Workload:● Critical Appraisal of Scientific Papers (seminar, 2 SWS) ● 60 Hours private studies



Qualification-goals/Competencies:● Students have the communication competency to elucidate the rationale for evidence-based medicine● Students have the communication competency to elucidate the rationale for and the principles of critical appraisal● Students have the communication competency to elucidate the importance of assessing the reporting quality of a study and the

quality of the study design● Students have the methodological competency to read and critically appraise scientific papers



Teacher:● Institute for Social Medicine and Epidemiology

● PD Dr. rer. nat. Annika Waldmann● Dr. med. Maria Raili Noftz


Notes:

Only for IB.Requirements: All modules of the 1st and 2nd semesters MSc Infection Biology must be passed.

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http://www.sozmed.uni-luebeck.de/

http://univis.uni-luebeck.de/form?__s=2&dsc=anew/tel_view&pers=mf/sozmed/sozmed/waldma&anonymous=1&founds=mf/sozmed/sozmed/waldma&sem=2014w&tel_nosem=1&__e=477

http://univis.uni-luebeck.de/form?__s=2&dsc=anew/tel_view&pers=mf/sozmed/sozmed/noftzm&anonymous=1&founds=mf/sozmed/sozmed/noftzm&sem=2014w&tel_nosem=1&__e=477

Module Guide

LS5200 TB - Module part: Tuberculosis (Tuberculos)




Classes and lectures: Workload:● Tuberculosis (seminar, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Insight in current TB research● Disease aspects and pathogenesis aspects of TB● Characterization of immune responses in TB● Details on the cell biology of the TB agent● Cellular pathways of host responses to TB agent including genetic susceptibilities● The microbiology of the TB agent● Cutting edge approaches and methods to study TB at immunological, cell biological and microbiological levels●

Qualification-goals/Competencies:● You can explain the infection and disease particularities of TB and its causative agent.● You can discuss the different intracellular pathways in anti-TB host responses and inflammation.● You can describe the immune response players in TB regarding protection and pathogenesis.● You can list the approaches for therapy and prophylaxis in TB● You can present research data to your peers and critically discuss cutting edge research using TB as model infection.● You are aware of the currently unsolved questions in TB research and actual approaches to answer them.● You develop the capacity to phrase strategies to tackle such questions experimentally.




● Prof. Dr. Ulrich Schaible

Literature:● : Cutting edge primary literature and reviews


Notes:

for MLS and IB.All modules of the 1st and 2nd semesters MSc Infection Biology must be passed.

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Module Guide

LS5200 VHI - Module part: Virus Host Interaction (VirHostIn)




Classes and lectures: Workload:● Virus Host Interaction (seminar / practical course / exercise, 2


Contents of teaching:● Characteristics of viral infections● Concepts of virus host interactions● Concepts of virus entry● Differences between enveloped and non-enveloped viruses● The role of viral entry receptors on susceptibility, trafficking, and tropism● Advanced molecular and virological methods

Qualification-goals/Competencies:● Can explain basic concepts of viral infections● Can explain methods to study virus host interactions● Can explain different concepts of viral entry● Can explain entry mechanisms of enveloped and non-enveloped viruses● Can apply basic and advanced molecular and virological methods● Are familiar with biological safety of a BSL1 and BSL2 laboratory● Can discuss and present scientific primary literature● Can communicate scientific results

Grading through:● continuous, successful participation in course● presentation



● Prof. Dr. rer. nat. Stefan Taube● MitarbeiterInnen des Instituts

Literature:● Flint, S.J., Racaniello, V.R., Enquist, L.W., Skalka, A.M., Flint, S.J., Enquist, L.W., Racaniello, V.R., and Skalka, A.M.: Principles of virology -

ASM Press, 2009


Notes:


46



Module Guide

LS5200 ZB - Module part: Cell Biology of the Senses (ZellBioSen)




Classes and lectures: Workload:● Cell Biology of the Senses (seminar, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Cellular and molecular mechanisms that enable our perception of the outside world● Impaired cell and tissue function and genetic diseases as causes of sensory failures● Introduction to cell physiology and neurobiology of the senses (signal processing)● Diseases and sensory failures: human genetic disorders and animal models● Techniques: in vivo imaging, electrophysiology, knockout mice, molecular techniques

Qualification-goals/Competencies:● Survey of cellular & molecular mechanisms of human sensory perception● Survey of specialized cell types involved in signal perception and processing● Impaired cell and tissue function and genetic diseases as causes of sensory failures● Critical reading and discussion of scientific primary literature● Presentation of scientific results

Grading through:● continuous, successful participation in course● presentation of original research papers



● Prof. Dr. rer nat. Rainer Duden

Literature:● : The Cell Biology of the Senses - Sonderheft des �Journal of Cell Biology� 2011● : - http://jcb.rupress.org/cgi/collection/rss?coll_alias=cell_biol_of_senses● : Primary literature for student presentations will be announced at the start of the module


Notes:

For ML and IB.Requirements: All modules of the 1st and 2nd semesters MSc Infection Biology must be passed.

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Module Guide

LS5200 ZD - Module part: Experimental Immunology (ExpImmuno)




Classes and lectures: Workload:● Experimental Immunology (lecture, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Animal models● Organisation and function of the immune system● Pathogens● The unspecific immune system● B lymphocytes and antibodies● T cells● Effector mechanims against infectious agents● Inflammation● Allergy● Tumour immunology● Tolerance and autoimmunity● Immun defects● Vaccination● Ethics in animal experiments● Biometric design and statistical evaluation of immunological experiments

Qualification-goals/Competencies:● Immunological topics should be discussed on the basis of experimental examples● Relevant scientific publications should be comprehended in an accompanying seminar

Grading through:● attendance, >90%● presentation



● Dr. rer. nat. Christoph Hölscher● Dr. rer.nat. Hanna Erdmann

Literature:● Kenneth Murphy: Janeway's Immunobiology - Taylor and Francis Ltd., 2011, ISBN-10:0815342438


Notes:

For both MLS and IB.Prerequisites: Passed all modules of the 1. and 2. semester MSc

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Module Guide

LS5200 ZE - Module part: Advanced course in Immunology (ImmunoFort)




Classes and lectures: Workload:● Immunology for higher levels (seminar, 2 SWS) ● 60 Hours private studies


Contents of teaching:● To understand the structure and function of lymphatic organs● Overview on the analysis and role of the T- and B-cell receptors● Overview on next- generation- sequencing● Critical appraisal on research literature● Overview on mechanisms and pathogenesis of human pathogens

Qualification-goals/Competencies:● They can describe the structure and function of lymphatic organs● They can explain the processes of antigen presentation and T cell activation● They can list T and B cell subsets and can describe their functions● They can describe the organization of the T and B cell antigen recognition receptors● They can illustrate the role of the T and B cell receptor repertoires in diseases● They can explain methods for analyzing the T and B cell receptor repertoire (Next-Generation-Sequencing)

Grading through:● Oral presentation and written report


Teacher:● Institute of Anatomy

● Dr. rer. nat. Kathrin Kalies● Prof. Dr. med. Jürgen Westermann

Literature:● Fred Rosen, Raif Geha: Case studies in Immunology: a clinical companion - Garland Science Publishing, 3rd edition● : Reviews, scientific articles


Notes:


49



http://www.anat.uni-luebeck.de/index.php?id=69

Module Guide

LS5200 ZF - Module part: Gene Expression (Genregu)




Classes and lectures: Workload:● Gene Expression (seminar / exercises, 2 SWS) ● 60 Hours private studies


Contents of teaching:● Definition of the terms �genome, transcriptome, proteome, promoterome, phenome, and exome● Principle of gene regulation on the DNA, RNA and protein level● RNA-Polymerase II (RNAP II): role, function of its subunits, and regulation● Basal transcription versus regulated transcription; factors in the respective processes● Significance of covalent modifications of promoters and histones● Examples of gene regulation in defined tissues and cell types, as well as during the processes of ontogenesis and inflammation● Examples for the regulation of transcription factor activity by ligand binding, phosphorylation and proteolytic cleavage: CREB,

NFκB, Notch, nuclear receptors (RAR, VDR, ER, TR).

Qualification-goals/Competencies:● They know the terms �genome, transcriptome, proteome, promoterome, phenome, and exome� and can explain their role for current

research strategies● They can explain the principles of gene regulation on the DNA, RNA and protein level● They can name the roles and functions of the RNAP II and can describe its regulation● They can list the differences between basal and regulated transcription. Moreover, they can specify and evaluate the various types of

modifications of promoters and histones in these processes● They know examples for the regulation of gene expression during ontogenesis and in defined tissues and cells (including the immune

system)● They can explain the regulation of transcription factors by ligand binding, phosphorylation, as well as non-specific and specific

proteolytic cleavage●

Grading through:● Oral presentation and exercise with written report


Teacher:● Department of Neurosurgery

● PD Dr. rer. nat. Christina Zechel

Literature:● : Originalliteratur und Reviews zu den einzelnen Themenabschnitten


Notes:

For both MLS and IB.Prerequisites: Passed all modules of the 1. and 2. semester MSc

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http://www.neurochirurgie.uni-luebeck.de/

Module Guide

LS5200 ZK - Module part: Cellular Microbiology & Inflammatory Diseases (CellInfDis)




Classes and lectures: Workload:● Cellular Microbiology & Inflammatory Diseases (seminar, 2


Contents of teaching:● Lecture: Cell Biology of inflammatory and infectious diseases: how pathogens maipulate the host cell�s membranes, organelles,

cytoskeleton, signalling and cell-cycle● Host-Pathogen Interactions● Bacterial toxins and mechanisms of intoxication● ER stress associated with infectious and inflammatory diseases●

● Practical part:● Live cell fluorescence microscopy to study the action of AB5 toxins on mammalian cells

Qualification-goals/Competencies:● Understanding of mechanisms leading to infectious and inflammatory diseases● Critical reading and discussion of primary scientific literature● Presentation of scientific results

Grading through:● continuous, successful participation in course● presentation of original research papers



● PD Dr. Nauk Irina Majoul

Literature:● Cossart, Boquet, Normark, and Rappuoli: Cellular Microbiology, - ASM Press, 2nd Edition 2005, ISBN: 1-55581-302-X● : - Primary literature for student presentations will be handed out at the beginning of the course


Notes:


51



Module Guide

LS5200 ZM - Module part: Molecular Biology of Virus Infections of Cythopathogenic and Non-Cytopathogenic

Viruses (MolbioVir)




Classes and lectures: Workload:● Molekular Biology of Virus infections of cythopathogenic and

non-cytopathogenic viruses (seminar / exercises, 2 SWS)● 60 Hours private studies● 30 Hours in-classroom work


●

●

●

●

●

●

Qualification-goals/Competencies:● They can explain principles of cytopathic effects in viral infections● They can list and explain basic methods to characterize virus- host-interactions● They can apply different RNA techniques to analyze viral infections● They can use and explain current methods of virus detection in cell culture● They can name the necessary safety requirements to work under BSL2 conditions with viral pathogens● They can work with stable cell lines and explain methods for their characterization

Grading through:● Oral presentation and written report



● Prof. Dr. rer. nat. Norbert Tautz● Dr. rer. nat. Olaf Isken

Literature:● S.J. Flint et al.: Principles of Virology: Molecular Biology, Pathogenesis, and Control of Animal Viruses - American Society Microbiology,

February 2009, 3rd Ed., ISBN: 978-1-55581-443-4


Notes:


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Module Guide

LS5200 ZP - Modle part: Inflammatory skin deseases: from bench to bedside (InSkinD)




Classes and lectures: Workload:● Inflammatory skin diseases: From bench to bedside (and back)

(seminar with practical exercises, 2 SWS)● 60 Hours private studies● 30 Hours in-classroom work

Contents of teaching:● Clinical presentation, diagnosis and current treatment options of autoimmune skin blistering diseases● In vitro and ex vivo models of AIBD● Animal models of AIBD● Isolation of immunoglobulin● Practical work: Isolation and characterization of immune IgG, and coating of 96-wells with immune complexes● Practical work: Activation of human neutrophils with immune complexes and other activators● Preparation of student presentations on in vitro models of inflammatory skin diseases such as psoriasis, allergic contact dermatis,

atopic dermatitis

Qualification-goals/Competencies:● They will obtain an overview of the clinical presentation, pathogenesis, diagnosis and treatment of inflammatory skin diseases● They will obtain in depth knowledge of the clinical presentation, pathogenesis, diagnosis and treatment of autoimmune skin blistering

diseases● They will learn (and perform) how to isolate IgG from serum● They will learn (and perform) how to isolate neutrophils from blood● They will learn (and perform) how to activate and evaluate the activation of human neutrophils

Grading through:● Recitation● protocols


Teacher:● Department of Dermatology, Allergology and Venerology

● Prof. Dr. med. Ralf Ludwig● Prof. Dr. med. Saleh Ibrahim

Literature:● : Original papers and reviews to the above topics


Notes:

For MLS and IB.Prerequisites: Passed all modules of the 1. and 2. semester MScOne week in March

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Module Guide

LS5200 ZR - Modle part: Animal experimentation: A comprehensive introduction into laboratory mice

(AnimalExp)




Classes and lectures: Workload:● Animal experimentation: A comprehensive introduction into

laboratory mice (seminar with practical exercises, 2 SWS)● 60 Hours in-classroom work● 30 Hours private studies

Contents of teaching:● Commonly used mouse stains● Generation of knock-out mice● Selected mouse models of AIBD● Isolation of immunoglobulin● Practical work: Isolation and characterization of immune IgA● Practical work: Mouse handling, including injection techniques● Preparation of student presentations on mouse models of inflammatory skin diseases (Ibrahim, Ludwig)

Qualification-goals/Competencies:● They will obtain an overview of commonly used mouse strains● They will learn how to generate transgenic mice● They will learn (and perform) isolation of IgA from immune-serum● They will learn how to handle laboratory mice (with a focus on injection techniques)● They will obtain an overview of mouse models of inflammatory skin diseases




● Prof. Dr. med. Ralf Ludwig● Prof. Dr. med. Saleh Ibrahim

Literature:● : Original papers and reviews to the above topics


Notes:


54



Module Guide

LS5200 ZS - Module part: Serological and histopathological diagnostic of inflammatory diseases (SeroHist)




Classes and lectures: Workload:● Serologically and histopathologically diagnostic of

inflammatory diseases (seminar with practical exercises, 2 SWS)● 60 Hours private studies● 30 Hours in-classroom work

Contents of teaching:● Clinic, diagnosis and therapy of chronic inflammatory skin diseases● Serologically and histopathologically methods for the analysis of cutaneous autoimmune diseases● Practical work: serologically analysis (ELISA, detection of autoantibodies and immunoblotanalysis)● Practical work: Detection of tissue bound autoantibodies (IgG, C3c) via direct immunofluorescence microscopy● Presentation: methods for the diagnosis of cutaneous autoimmune diseases

Qualification-goals/Competencies:● You have an overview about methods for the diagnosis of cutaneous autoimmune diseases● You can perform serologically analysis (e.g. ELISA, detection of autoantibodies and immunoblotanalysis)● You can detect tissue bound autoantibodies (IgG, C3c) via direct immunofluorescence microscopy




● Prof. Dr. med. Dr. rer. nat. Enno Schmidt


Notes:

For MLS and IB.Prerequisites: Passed all modules of the 1. and 2. semester MSc.One week in March

55



Module Guide

LS5200 ZT - Module part: Molecular Genetics of inflammatory diseases (MolGenIn)




Classes and lectures: Workload:● Molecular Genetics of inflammatory diseases (seminar with

practical exercises, 2 SWS)● 60 Hours in-classroom work● 30 Hours private studies

Contents of teaching:● Environmental and genetically reasons for chronic-inflammatory diseases● Animal models for the investigation of autoimmune diseases● Ex vivo and in silico analysis in human genetics● Practical work: Isolation of human and bacterial DNA, SNP genotyping● Practical work: Genome-wide-association mapping and QTL mapping● Presentation: Methods of analysis in human genetics

Qualification-goals/Competencies:● You have an overview about human genetics methods for chronic inflammatory diseases● You are able to extract DNA and to genotype samples● You can perform bioinformatically and statistically analysis (e.g. GWAS, QTL mapping diversity analysis)




● Prof. Dr. med. Saleh Ibrahim


Notes:

For MLS and IB.Prerequisites: Passed all modules of the 1. and 2. semester MSc.One week in March

56



Module Guide

LS5205 - Consolidating in Infection Biology (ConsoleIB)


1 Semester each winter semester 6 (Typ B)

Course of study, specific field and term:● Master Infection Biology (compulsory), Interdisciplinary internships and consolidation courses, 3rd semester

Classes and lectures: Workload:● 2 courses of list LS5200... (see remarks) (depends on the chosen

courses, 1 SWS)● 120 Hours private studies● 60 Hours in-classroom work

Contents of teaching:● See special plan of the course located on the IB website.

Qualification-goals/Competencies:● Ability, to understand and reproduce the specific knowledge of the topics described in

Grading through:● as announced by examiner


Teacher:● Universitätsklinikum S-H● Research Center Borstel● All institutes of the University of Lübeck

● Alle Dozentinnen/Dozenten der UzL


Notes:

Two courses starting LS5200� from the list below must be passed (no grades).

(Consists of LS5200 ZB, LS5200 ZC, LS5200 ZD, LS5200 ZE, LS5200 ZF, LS5200 ZK, LS5200 ZM, LS5200 ZP, LS5200 ZR, LS5200 ZS, LS5200ZT, LS5200 IB1, LS5200 IB2, LS5200 IB3, LS5200 IB4, LS5200 SA, LS5200 SB, LS5200 SC, LS5200 SE, LS5200 SG, LS5200 SI, LS5200 SK)

57



Module Guide

LS5995 - Master Thesis Infection Biology (MScThesis)


1 Semester each semester 30

Course of study, specific field and term:● Master Infection Biology (compulsory), Masterthesis, 3rd and 4th semester

Classes and lectures: Workload:● Practical work (practical course, 1 SWS)● Authoring of the Master Thesis (supervised self studies, 1 SWS)● Colloquium (presentation (incl. preparation), 1 SWS)


Contents of teaching:● Scientific project in the field of infection biology

Qualification-goals/Competencies:● Competence and ability to solve a preformulated more complex scientific problem in a defined period of time and to document with

respect to good scientific practice and present and critically defend the experimental results.

Grading through:● written exam, oral presentation, and defence of the experiment´s results


Teacher:● Institutes of natural science● Research Center Borstel

● Alle prüfungsberechtigten Dozentinnen/Dozenten des Studienganges


Notes:

Prerequisites: Minimum of 70 ECTS.If the Master thesis is done externally (outside our university) the student has to choose a licensed lecturer (see PO) of our university as asecond instructor who will be First Examiner in the examination.

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Documents

Module Guide for the Study Path - uni-luebeck.de Guide for the Study Path ... Viva Voce written or oral ... Can present a molecular virology based paper in a journal club format