MOSQUITO OL-TRAP 18 May 20152DOST School-based O/L Trap Program Objective General Provide the scientific framework of the OL Trap Program of Dengue vector control
MOSQUITO OL-TRAP 18 May 20153DOST School-based O/L Trap Program Objective Specific Explain the concept of vector control Explain dengue vector control Explain Integrated Vector Control (IVC) and Integrated Vector Management (IVM) Discuss the components of IVC from vector biology to control measures
MOSQUITO OL-TRAP 18 May 20154DOST School-based O/L Trap Program Objective Specific Describe vector surveillance as it applies to dengue OL Trap in a classroom setting Answer the fourteen questions enumerated in the situationer
MOSQUITO OL-TRAP 18 May 20155DOST School-based O/L Trap Program Objective Specific Identify the following Mosquito eggs in a lawanit paddle Ovitrap (black tumbler and paddle) Mosquito larva Pupae Positive/negative trap Adults Entomological forms (Ovitrap/OL Trap) Pellets
MOSQUITO OL-TRAP 18 May 20156DOST School-based O/L Trap Program Situationer Urban / semi-urban setting Big public elementary school in City X 3-story building (~30-35 feet) 10 classrooms per floor 16x20 feet per room) 50-55 pupils occupy one classroom from 8am-5pm
MOSQUITO OL-TRAP 18 May 20157DOST School-based O/L Trap Program Situationer ~ 1,200-1,500 pupils No electric fans in the classrooms One aircon and/or electric fan at principals office Houses near the school Presence of flower vases, water containers Click Here
MOSQUITO OL-TRAP 18 May 20158DOST School-based O/L Trap Program Questions How many ovitraps/OL Traps are needed to be installed in the elementary school? Where should the traps be installed? When should the traps be installed? How frequent should the traps be installed?
MOSQUITO OL-TRAP 18 May 20159DOST School-based O/L Trap Program Questions What is the procedure to be followed in installing the traps? In servicing the traps? What is the meaning of servicing the traps? Who will install and service the traps?
MOSQUITO OL-TRAP 18 May 201510DOST School-based O/L Trap Program Questions Who will monitor and check the traps? Monitor Empty tumbler Missing tumbler/paddle Check If positive/negative? Is there a form to be filled-out?
MOSQUITO OL-TRAP 18 May 201511DOST School-based O/L Trap Program Questions What index needs to be obtained? What formula is to be used? What is the meaning of the index obtained? Who will record and compute for the index?
MOSQUITO OL-TRAP 18 May 201512DOST School-based O/L Trap Program Questions To whom should the data be submitted to? Why? For how long will this ovitrapping be done?
MOSQUITO OL-TRAP 18 May 201513DOST School-based O/L Trap Program Whats inside? Vector Surveillance Prevention and Control
MOSQUITO OL-TRAP 18 May 201514DOST School-based O/L Trap Program Vector An arthropod (insects, mites, ticks) that are capable of: acquiring, supporting the development and transmitting a pathogenic agent (bacteria, viruses, parasites) from one host to another host Example: mosquito Aedes sp. transmitting dengue virus
MOSQUITO OL-TRAP 18 May 201515DOST School-based O/L Trap Program Vector Control suppression of a target population by using measures to alter the vector reproductive capacity or potential (Vector Genetics Course, 2010, India)
MOSQUITO OL-TRAP 18 May 201516DOST School-based O/L Trap Program Dengue Vector Control Application of various anti-DEN mosquito measures to alter the life cycle of the mosquito
MOSQUITO OL-TRAP 18 May 201517DOST School-based O/L Trap Program Dengue Vector Control Specific Targets Prevent and cut dengue transmission by: Targeting larval stage through Trapping Larviciding Container management Environmental sanitation
MOSQUITO OL-TRAP 18 May 201518DOST School-based O/L Trap Program Dengue Vector Control Specific Targets Prevent and cut dengue transmission by: Targeting adult stage through Adulticiding Space-spraying Aerosol treatment Genetic manipulation of reproductive capacity of mosquito
MOSQUITO OL-TRAP 18 May 201519DOST School-based O/L Trap Program Dengue Vector Control Specific Targets Prevent and cut dengue transmission by: Prevention of man-mosquito contact curtains Screening of doors and windows Long pants Repellants
MOSQUITO OL-TRAP Integrated Vector Control Combination of 2 or more vector control activities supplementary to each other towards reduction of dengue incidence 18 May 201520DOST School-based O/L Trap Program
MOSQUITO OL-TRAP Integrated Vector Management utilization of technical and managerial measures to bring about an effective degree of vector suppression to prevent and control transmission 18 May 201521DOST School-based O/L Trap Program
MOSQUITO OL-TRAP Components of IVC Vector biology/behavior Surveillance tool (Ovitrap/OL Trap surveillance index) Critical Threshold Density Index (effective degree of vector suppression [Ovitrap index] Control Measures Monitoring and evaluation 18 May 201522DOST School-based O/L Trap Program
MOSQUITO OL-TRAP 18 May 201523DOST School-based O/L Trap Program The Vector
MOSQUITO OL-TRAP Vector Biology 18 May 201524DOST School-based O/L Trap Program Aedes aegyptiAedes albopictus General characteristics - primary vector - involve in cases of epidemics - urbanized areas - lives indoors - secondary vector - maintains the virus in the environment - rural areas - lives outdoors Breeding habitat - confined to artificial containers indoors - prefers to breed outdoor, in natural containers Resting habits - rests in cool, dark corners of the house - rests outdoors in clearings and vegetations
MOSQUITO OL-TRAP Morphology 18 May 201525DOST School-based O/L Trap Program Aedes aegyptiAedes albopictus Size - 3-4 mm- same Color - dark brown to black- same Thorax - lyre-shaped white marks or scales - single white marks Abdomen - with white scales - with white and dark marks Legs - with white marks- same
MOSQUITO OL-TRAP Vector Characteristics Biting Habits daybiters with two peaks of biting time: 1 to 2 hours after sunrise 1 to 2 hours before sunset only female mosquitoes bite because of the requirement of blood for oviposition males do not bite but feed on plant juices and nectar of flowers man is the preferred host but in his absence, lower vertebrates serve as substitute 18 May 201526DOST School-based O/L Trap Program
MOSQUITO OL-TRAP Vector Characteristics Flying Habit do not fly great distances farthest flight distance is within 200 to 400 meters radius from their breeding places Adults found around 50 meters from the breeding sources 18 May 201527DOST School-based O/L Trap Program
MOSQUITO OL-TRAP Bionomics at temperatures 40 o C, no developmental process takes place females mates with male only once Blood meal takes place after 48 hours from emergence and after mating for egg production 18 May 201528DOST School-based O/L Trap Program
MOSQUITO OL-TRAP Bionomics oviposition takes place about 2 to 5 days from blood engorgement prefers dark-colored background for oviposition lays egg 3 to 4 times in its lifetime 60 to 100 eggs per batch Survival potential: 20-30 days 18 May 201529DOST School-based O/L Trap Program
MOSQUITO OL-TRAP Life Cycle (10-12 days) 18 May 201530DOST School-based O/L Trap Program Eggs: - Black and oval in shape - Laid singly above water surfaces of containers - Without float - Viability: 6 months to 1 year due to the presence of chorion 2 - 3 days Larva: - Feeding stage - Breeds in clean and non-polluted water - Short and stout siphon with one pair of hair tuft - Rests at an angle to the water surface 6 - 8 days Pupa -Non-feeding stage -Breeding trumpet is long, slender with narrow opening 1 - 2 days Adult -Maxillary pulps shorter than probosis -Wings uniformly grayish black -Body and legs are black with distinctive white patches throughout -Thorax has markings
MOSQUITO OL-TRAP 18 May 201531DOST School-based O/L Trap Program SurveillanceSurveillance
MOSQUITO OL-TRAP Vector Surveillance Collection and interpretation of data which can be used to guide mosquito vector control operations Establish baseline Presence or absence of vector Species? Density? Changes? 18 May 201532DOST School-based O/L Trap Program
MOSQUITO OL-TRAP Dengue Surveillance Tool Classical OVITRAP/LARVITRAP (black tumbler, paddle, water) Modified OVITRAP is the OLTRAP (black container, paddle, water and organic larvicide) 2 adults (females) emerging per day (Malaysian experience) 18 May 201533DOST School-based O/L Trap Program