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Nucleotide Structure: Pentose sugar

Nucleotide Structure: Pentose sugar

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Nucleotide Structure: Pentose sugar. Nucleotide Structure: Nitrogenous Bases. DNA Linkage. Phosphate group/phosphodiester bond Nucleotides contain one, two, or three phosphate groups. *nucleoside *nucleotide. Nucleic Acid Polarity. *Top of molecule has free 5’-phosphate group - PowerPoint PPT Presentation

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Page 1: Nucleotide Structure: Pentose sugar

Nucleotide Structure: Pentose sugar

Page 2: Nucleotide Structure: Pentose sugar

Nucleotide Structure: Nitrogenous Bases

Page 3: Nucleotide Structure: Pentose sugar

DNA Linkage

• Phosphate group/phosphodiester bond• Nucleotides contain one, two, or three

phosphate groups

*nucleoside *nucleotide

Page 4: Nucleotide Structure: Pentose sugar

Nucleic Acid Polarity

*Top of molecule hasfree 5’-phosphate

group

This is the 5’ end

*Bottom has a free 3’hydroxyl group

This is the 3’ end

Page 5: Nucleotide Structure: Pentose sugar

*Early 1950’s Rosalind Franklin

*1953 Watson and Crick

Structural analysis of DNA

Page 6: Nucleotide Structure: Pentose sugar

Complementarity

Page 7: Nucleotide Structure: Pentose sugar

DNA Structure:

Page 8: Nucleotide Structure: Pentose sugar
Page 9: Nucleotide Structure: Pentose sugar

Brief Review of DNA Replication:

Page 10: Nucleotide Structure: Pentose sugar

Nearest Neighbor Analysis (Kornberg 1956):

In vitro DNA synthesis

•Template DNA

•DNA polymerase

•dNTPs*dTTP*dCTP *dGTP*dATP

•Allow DNA replication to take place

•Denature DNA

•Treat with micrococcal nuclease-

yields mononucleotides with 3’ terminal phosphates

Page 11: Nucleotide Structure: Pentose sugar

A

C

G

T

T

C

ssDNA:

*

ssDNA treated with micrococcal nuclease:

A

C

G

T

T

C

*

Page 12: Nucleotide Structure: Pentose sugar

Exercise:

List all possible dinucleotides (analysis of nearest 5’ neighbor)

Which must be present in equal frequencies if the DNA strands are:

Parallel ? Antiparallel?