Overview of Genomic DNA Purification 1 · 2013-04-11 · Overview of Genomic DNA Purification e-mail:[email protected] 1 DP318 tianamp Blood Dna Kit (0.1-1 ml) Genomic DNA Purification

Genomic DNA Purification Overview of Genomic DNA Purification 1Genomic DNA Purification Products

TIANcombi DNA Lyse & Amp PCR Kit 7

TIANamp Bacteria DNA Kit 8

Plant Genomic DNA Kit 9

DNAsecure Plant Kit 10

DNAquick Plant System 11

TIANamp Feedstuff Animal DNA Kit 12

DNA Extraction Kit for Food 13

TIANamp Marine Animal DNA Kit 14

TIANamp Yeast DNA Kit 15

TIANamp Virus DNA / RNA Kit 16

TIANamp Stool DNA Kit 17

TIANamp Genomic DNA Kit 18

TIANamp Blood DNA Kit (0.1 - 1 ml) 19

TIANamp Blood DNA Midi Kit (0.5-3 ml) 20

TIANamp Blood DNA Maxi Kit (3-10 ml) 21

RelaxGene Blood DNA System (0.1 - 20 ml) 22

TIANquick FFPE DNA Kit 23

TIANamp FFPE DNA Kit 24

TIANamp N96 Blood DNA Kit 25

TIANamp Swab DNA Kit 26

TIANamp Micro DNA Kit 27

Magnetic Genomic DNA Kit 28

Ordering Information 29

RNA PurificationOverview of RNA Purification 30RNA Purification Products

TRNzol Reagent 37

TRNzol-A+ Reagent 38

RNAplant plus Reagent 39

RNAsimple Total RNA Kit 40

RNAprep pure Kit (For Plant) 41

RNAprep pure Kit (For Tissue) 42

RNAprep pure Kit (For Cell / Bacteria) 43

RNAprep pure Kit (For Blood) 44

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RNAprep pure Kit (For Micro Sample) 45

RNAprep pure FFPE Kit 46

TIANamp Virus DNA / RNA Kit 47

TIANamp Virus RNA Kit 48

Magnetic Virus DNA / RNA Kit 49

TIANamp DNA / RNA Isolation Kit 50

TIANamp DNA / RNA / Protein Isolation Kit 51

RNAclean Kit 52

RNAstore Reagent 53

RNALock Reagent 54

RNAsafe (RNase Inhibitor) 55

RNasin(RNase Inhibitor) 55

miRcute miRNA Isolation Kit 56


Plasmid DNA Purification

Plasmid DNA Purification Products

TIANprep Mini Plasmid Kit 59

TIANpure Mini Plasmid Kit 60

TIANprep Midi Plasmid Kit 60

TIANpure Midi Plasmid Kit 61

HighPure Maxi Plasmid Kit 62

EndoFree Maxi Plasmid Kit 63

TIANprep Highpure N96 Plasmid Kit 64

TIANprep Rapid N96 Plasmid Kit 65

TIANprep Yeast Plasmid DNA Kit 66


PCR Cleanup & Gel ExtractionPCR Cleanup & Gel Extraction Products

TIANquick Midi Purification Kit 69

TIANquick Maxi Purification Kit 70

TIANquick N96 Purification Kit 71

TIANquick Oligo Purification Kit 72

Universal DNA Purification Kit 73

TIANgel Midi Purification Kit 74

TIANgel Maxi Purification Kit 75


DNA Molecular Weight Marker TIANGEN 2×PCR Master Mix

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Overview of Genomic DNA Purification

www.tiangen.com e-mail:[email protected]

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tionDP318 tianamp Blood Dna Kit (0.1-1 ml)

DP319 Relaxgene Blood Dna System (0.1 -20 ml)

DP332 tianamp Blood Dna midi Kit (0.5-3 ml)

DP314 tianamp n96 Blood Dna Kit

DP333 tianamp Blood Dna maxi Kit (3-10 ml)

DP304 tianamp genomic Dna Kit

DP322 tianamp Swab Dna Kit

DP316 tianamp micro Dna Kit

DP329 magnetic genomic Dna Kit

DP330 tianquick FFPe Dna Kit

DP331 tianamp FFPe Dna Kit

DP315 tianamp Virus Dna/Rna Kit

DP328 tianamp Stool Dna Kit

Genomic DNA Purification Productsgenomic Dna purification technology provides a method for purification of high-quality genomic Dna from wide range of samples such as blood, bacteria, yeast, animal tissues and cells, plant tissues and cells, feed, medical samples and deep-processed food. Purified genomic DNA is up to 50kb in size, with an average length of 20-30 kb. Using the right purification product for each application is key to your success. to ensure you get the

Blood

Blood, tissue, cell

Swab

micro Samples

FFPe Samples

DP305 Plant genomic Dna Kit

DP320 Dnasecure Plant Dna Kit

DP321 Dnaquick Plant System

DP302 tianamp Bacteria Dna Kit

DP307 tianamp Yeast Dna Kit

Kg201 tiancombi Dna Lyse&amp PcR Kit

DP323 tianamp Feedstuff animal Dna Kit

DP324 tianamp marine animal Dna Kit

DP326 Dna extraction Kit for Food

Plant

Virus

Bacteria

Yeast

animal, Plant & Bacteria

Stool

Detection

clinical/animal Samples

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best results, tiangen offers a wide range of different products – each designed to effectively purifying genomic Dna from a certain sort of samples. to help you choose the best product for your need, the following section summarizes the characteristics and suited applications of each tiangen’s genomic Dna products.



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Product featuresgenomic Dna purification kits enable rapid purification of pure high-molecular-weight Dna without expensive instruments. there is no need of toxic organic reagents such as phenol, and chloroform, etc. This makes purification of genomic DNA a simple and safe process, suited for parallel processing of multiple samples.

Applications of purified DNA■ Library construction■ Genetic maps■ Southern Blot■ PCR

Applicable samplesPlant / blood / tissues / cultured cells / mouse tail / serum / plasma / mouthwash swab / saliva / feed / stool / deep-processed food / yeast / virus / bacteria / FFPe, etc.

Genomic DNA kit feature comparison chart

Cat.Num. Product Time Safety (For reference)

Typical Downstream Applications (non-toxic)

KG201 TIANcombi DNA Lyse&Amp PCR Kit 20 min PCR only √

DP302 TIANamp Bacteria DNA Kit 60 min PCR, qPCR, Digestion √

DP305 Plant genomic Dna Kit 60 min PcR, qPcR, Digestion, Southern Blot

DP320 Dnasecure Plant Dna Kit 60 min PcR, qPcR, molecular markers,

Library construction, Southern Blot √

DP321 Dnaquick Plant System 60 min PcR, molecular markers,

Library construction, Southern Blot √

DP307 TIANamp Yeast DNA Kit 60 min PCR, Digestion, Library Construction √

DP323 TIANamp Feedstuff Animal DNA Kit 60 min PCR, qPCR √

DP324 TIANamp Marine Animal DNA Kit 60 min PCR, Digestion, Library Construction √

DP326 DNA Extraction Kit for Food 60 min PCR √

DP315 TIANamp Virus DNA/RNA Kit 60 min PCR, qPCR √

DP328 TIANamp Stool DNA Kit 60 min PCR, qPCR √

DP304 TIANamp Genomic DNA Kit 60 min PCR, Digestion, Southern Blot √

DP318 TIANamp Blood DNA Kit ( 0.1-1 ml ) 60 min PCR, qPCR, Digestion, Southern blot √

DP319 Relaxgene Blood Dna System (0.1-20 ml) 60 min PCR, Southern Blot √

DP332 tianamp Blood Dna midi Kit 60 min PcR, Digestion, Southern Blot √

DP333 tianamp Blood Dna maxi Kit 60 min PcR, Digestion, Southern Blot √

DP316 TIANamp Micro DNA Kit 60 min PCR, qPCR √

DP329 Magnetic Genomic DNA Kit 45 min PCR, qPCR, Digestion √

DP322 TIANamp Swab DNA Kit 90 min PCR, qPCR √

DP330 tianquick FFPe Dna Kit 60 min PcR, SnP, StR, Pharmacogenomics analysis √

DP331 tianamp FFPe Dna Kit 3 hours PcR, qPcR, SnP, StR,

Pharmacogenomics analysis

*qPcR: Quantitative real-time PcR



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Genomic DNA purification principles■ ensure the integrity of nucleic acid (nucleic acid integrity is

the base for further functional study).■ eliminate contaminants of large molecules (protein,

polysaccharide, lipids, and organic solvent) to ensure the smooth processing of downstream studies.

Genomic DNA purification methodsThe purification of DNA from cellular components can be divided into four stages:1. Homogenization2. Lysis3. Removal of proteins and other contaminants4. Recovery of Dna

Sample preparation

Genomic DNA Purification Technology

Preparation methods are varied for samples from different sources (bacteria, yeast, blood, animal tissues, plant tissues, and cultured cells), or the same source in different form (fresh blood, frozen blood, blood clots, and dried blood).

Requirements for sampleFresh samples or fresh samples stored at -20℃ or -80℃ immediately after collection are recommended. Repeated freeze-thaw circles should be avoided, as it led to reduced fragment lengths and low yields. collect samples in logarithmic phase for bacteria and yeast.

Sample pre-treatment methods■ Plant sample - Grinding in liquid nitrogen ■ Animal sample - Tissue homogenate, grinding in liquid nitrogen ■ Bacteria sample - Lysozyme treatment ■ Yeast sample - Glass beads grinding and lyticase treatment

Cell lysistiangen genomic Dna purification kits are based on SDS or ctaB methods to lyse cells, and obtain high molecular weight Dna fragments. You can choose the appropriate lysis methods for

Sample preparation cell lysis,release of Dna

Adsorption purification to removeprotein, lipids and polysaccharide

Removing salts and organic impurities

obtain pure genomic Dna

Dna binding to the silica membrane

wash

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different samples.

CTAB method■ Suitable for plant tissues and fungi.■ ctaB (hexadecyltrimethylammonium bromide) is a cationic

detergent which is able to dissolve the cell membrane and forms complex with nucleic acid. this complex is soluble in salt solution.

SDS method ■ Suitable for blood, cells, animal tissues, bacteria and yeast.■ SDS is an anionic detergent, it can lysis cells at high

temperature (55℃-65℃), denature proteins and precipitate chromosome.

Other lysis methods■ Physical methods - mechanical shear, sonication, grinding

homogenate.■ chemical methods - guanidine isothiocyanate, alkaline lysis.■ Enzymatic methods – protease K.

DNA isolation and purificationPurification Requirements

■ no organic molecules or high concentration of salts that will inhibit the activity of enzymes (Endonuclease, DNA polymerase).

■ Low residual of biological molecules such as protein, polysaccharide, and lipids contamination.

■ exclude Rna contamination from purified Dna and vice versa.

Purification methodThe purification of DNA usually begins with lysis, or breakdown of tissue or cells. this process is essential for the destruction of protein structures and allows for release of nucleic acids from the nucleus. adsorption material based technology has been used for purification of DNA and removing impurities. Silicon substrate adsorption material, anion exchange resin and beads are the main adsorption material. Silicon substrate adsorption material is widely used for nucleic acid purification because it has the advantages of fast, simple and no need of toxic organic compounds such as phenol, and chloroform. TIANGEN genomic DNA purification kits use silicon-based membrane technology to keep the integrity of DNA. The methods of purification have to be properly selected to optimize the yield and quality of the extracted DNA.

DNA purification Notesin order to ensure the purity and integrity of purified Dna:

■ Make sure the the purification process is simple enough and reduce harmful factors for damaging nucleic acids.

■ To reduce DNA degradation causing by chemical damage, keep pH value between 4.0 and 10.0 in experiments.

■ Prevent DNA from biodegradation. Various deoxy- is

ribonucleases can break down Dna and damage the primary structure of nucleic acids. Dnase need to be activated by mg2+, ca2+, therefore eDta or citrate can effectively inhibit Dnase activity. Buffers in tiangen Dna purification kit contain Dnase inhibitors to prevent Dna from degradation.

■ Reduce DNA degradation from physical force which is mainly mechanical shear stress; avoid repeated freeze-thaw cycles or exposure to high temperature of Dna samples.

DNA elutionDNA elution efficiency depends on the following factors:

Eluent compositionwhen adsorption material based technology is used for purification of Dna, the optimal pH value for Dna elution is between 7.0 and 8.5. tiangen genomic Dna purification kits use buffer te (pH 8.0) to elute Dna from silica membrane and preserve Dna sample. eDta concentration in te buffer is low and has very slight influence on endonuclease and Dna polymerase. there is no need of concerns for the te eluted Dna fragments in following downstream applications. (notes: Dna elution efficiency can be improved by incubating at 60-75℃for 10 minutes before te elution)

Elution volumethe following figures indicate that elution efficiency is low and unstable when the elution volume is below 30 µl; elution efficiency

Relationship between elution volume and recovery yield

Dna elution efficiency and yields are greatly improved by the second and third time elution

increase clution volume

Elution volume(μl)



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at 80% - 90% and maximum yield can be reached when the elution volume is between 50 µl and 200 µl. Buffer Pw should be completely removed before elution. centrifuge for 2 min and preserve under Rt for 5-10 min can efficiently eliminate organic solvent ethanol influence for downstream applications.

Elution time and frequencyafter adding elute buffer te, incubate at room temperature for 2-5 min to ensure complete Dna elution from silica-membrane and dissolution into buffer te. twice or thrice elution can be done to dissolve residual Dna on silica-membrane. this will greatly increase Dna elution efficiency and yield.

DNA storage

Storage buffer

Dna is stable under alkaline condition and usually preserved in te solution. te solution composition: 10 mm tris-Hci (tris forms strong buffer pair with hydrochloric acid); 1 mm eDta (eDta can chelate divalent metal ions, therefore inhibit Dna enzyme activity); pH 8.0 (alkaline condition can reduce Dna degradation). Buffer te of tiangen genomic Dna kit can effectively elute and serve

as long time preservation solution for Dna. Q: If water is applicable for long term preservation of DNA?A: nomal pH value of ddH2o is 7.0 and applicable for Dna

preservation. But sometimes laboratory prepared ddH2o is acidic and affect Dna elution from silica membrane. Long term preservation in acidic ddH2o leads to Dna degradation. therefore, buffer te is recommended for long term preservation of Dna solution.

Q: If the purified high molecular weight DNA needs to be precipitated and dried.A: genomic Dna molecular weight is too high and especially

hard to dissolve for dry products. Besides stirring dissolve method can cause Dna molecule damage, so there is no need to dry Dna products.

Storage temperature in order to prevent Dna degradation, extracted Dna fragments need to be aliquoted and preserved in refrigerator at -20℃ or -80℃. Dna can be preserved for more than 5 years at -80℃. avoid Dna degradation from repeated freeze-thaw cycles.

Genomic DNA Purification Products

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genomic Dna was isolated from different samples and amplified using tiancombi Dna Lyse&amp PcR Kit. (A) 10 mg plant leaves. the gene Zein of corn, Lectin of soybean and a 694 bp fragment in chromosome 12 of rice were amplified. (B) 800 µl bacteria overnight culture. the gene of 16s rRna was amplified. (C) 10 mg mouse tail. the gene of gaPDH was amplified. 5 µl PcR products were run on agarose gel. M: tiangen markerⅡ 1: Fresh leaves 2: Seeds P: Positive control G Positive: S. aureus G Negative: E. coli NTC: negative control.

TIANcombi DNA Lyse & Amp PCR KitFor ultra-fast and easy Dna purification from plant, bacteria and tissue samples and PcR reaction

Descriptiontiancombi Dna Lyse & amp PcR Kit uses a special buffer system for fast one-step Dna extraction and PcR from a wide range of starting materials, including plant tissues, seeds, bacteria and animal tissues. the kit contains all of the reagents required for DNA extraction and the PCR amplification reagent. Phenol extraction, ethanol precipitation, or grinding in liquid nitrogen are not required. this kit produces PcR-ready Dna for quick screening in as little as 20 minutes followed by high-performance PCR amplification.

Features■ Grinding in liquid nitrogen is not required. PCR-ready DNA can be purified from a

wide range of starting materials in 20 minutes.■ the kit is suited to a broad range of starting materials such as plant leaves,

seeds, bacteria (gram-positive and gram-negative bacteria), and animal tissues.■ no phenol / chloroform extraction, as low as 10 mg starting volume of samples is

accepted.■ the kit provides solutions for both Dna extraction and PcR.

Applications■ Fast screening by DNA extraction and PCR amplification

Ordering Information

Contents and Storage

Cat. no. QuantityKg201-02 50 prepsKg201-03 200 preps

Kit Components 50 preps 200 prepsLysis Buffer 12 ml 40 mlProteinase K(20 mg/ml) 500 µl 2 ml2×PcR Reagent 500 µl 2 mlgrinding Pestle 5 5

Store 2×PcR Reagent at -20℃ and other reagents at room temperature (15-25℃)

corn Soybean Rice

m mouse tail P

Experimental Example

m 1 2 P 1 2 P 1 2 P

m g Positive g negative ntc


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TIANamp Bacteria DNA KitSimple genomic Dna purification from gram-negative and gram-positive bacteria

Description tianamp Bacteria genomic Dna Kit is based on the silica membrane technology and a special buffer system for extracting gDna extraction from a wide range of gram-negative and gram-positive bacteria. this kit can also be used to purify genomic Dna from pathogenic microbes in food samples, such as Staphylococcus aureus, Vibrio cholerae, hemorrhagic Escherichia coli o157: H7, Listeria monocytogenes, Salmonella, Enterobacter sakazakii, etc. Up to 10 - 40 µg genomic DNA can be rapidly purified from 1-5 ml of culture. genomic Dna purified by the tianamp Bacteria Dna Kit is suitable for a variety of downstream applications, including PcR amplification, restriction endonuclease digestion and Southern Blot.

Features■ Genomic DNA can be purified from Gram-negative bacteria within one hour.■ The high pure gDNA can be used directly in downstream experiments, such as

PCR amplification, restriction endonuclease digestion and Southern Blot.

Applications■ Genomic DNA purification from Gram-negative and Gram-positive bacteria■ Genomic DNA purification from pathogenic bacteria in food

Required Reagents Ethanol (96% -100%); For Gram-positive bacteria: Lysozyme & Lysozyme buffer (20 mm tris, 2mm na2-eDta, 1.2% triton pH 8.0)

Typical DNA Yields




Cat. no. QuantityDP302-02 50 preps

Kit Components 50 prepsBuffer ga 15 mlBuffer gB 15 mlBuffer gD 13 mlBuffer Pw 15 mlBuffer te 15 mlProteinase K 1 mltianamp Spin columns cB3 50 collection tubes (2 ml) 50

Store at room temperature (15-25℃)

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genomic Dna was isolated from 1 ml overnight culture of different bacteria samples using tianamp Bacteria Dna Kit. 3 µl of 100 µl eluates were loaded per lane.M: TIANGEN λ DNA/Hind III Marker1: λ DNA2: E.coli3: S. epidermidis4: S. aureus

Starting Material Gram-negative bacteria Gram-positive bacterianumber of cells 2×108 cells/ml 3.5×108 cells/mlStarting Volume 1 ml 1 mlDna Yield 15-20 µg 6-13 µgoD260/oD280 1.7-1.9 1.7-1.9


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Plant Genomic DNA KitFor genomic Dna purification from powder or polysaccharide/ polyphenol rich plants

Description Plant genomic Dna Kit provides a fast, simple, and cost-effective genomic Dna extraction method for routine molecular biology laboratory applications. the kit is based on the silica membrane technology and a unique buffer to eliminate polysaccharide, polyphenol, and enzyme inhibitors. Plant genomic Dna Kit is ready-to-use to purify the genomic Dna from a wide variety of plants. Purified Dna is suitable for PcR, qPcR, restriction digestion and Southern Blot.

Features■ The procedure is quick and convenient within one hour.■ The high pure genomic DNA can be used directly in downstream experiments

such as PcR amplification, restriction endonuclease digestion and Southern Blotting.

Applications■ Suited to various plant tissues■ Particularly suited to polysaccharide-rich, polyphenol-rich plant tissues, and

plant powders

Required Reagents Liquid nitrogen, ethanol (96% -100%), Phenol-chloroform, Rnasea (optional)

Typical DNA Yields




Cat. no. QuantityDP305-02 50 prepsDP305-03 200 preps

Kit Components 50 preps 200 prepsBuffer gP1 40 ml 160 mlBuffer gP2 40 ml 160 mlBuffer gD 13 ml 52 mlBuffer Pw 15 ml 50 mlBuffer te 15 ml 60 mlSpin columns cB3 50 200collection tubes (2 ml) 50 200Store at room temperature (15-25℃)

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genomic Dna was isolated from 100 mg leaves of different plants using Plant genomic Dna Kit. 3 µl of 100 µl eluates were loaded per lane.m: TIANGEN λ DNA/Hind III Marker1: Fresh tomato leaves2: Fresh cotton leaves3: Fresh tea leaves4: Fresh strawberry leaves

Starting Sample Sample Amount Yield Range OD260/OD280

wheat 100 mg 25-30 µg 1.7-1.9Pine needles 100 mg 25-30 µg 1.7-1.9Potato 100 mg 4-6 µg 1.7-1.9tomato 100 mg 10-15 µg 1.7-1.9Strawberry 100 mg 10-15 µg 1.7-1.9tobacco 100 mg 20-25 µg 1.7-1.9Rice 100 mg 10-25 µg 1.7-1.9Soy 100 mg 20-30 µg 1.7-1.9corn 100 mg 20-30 µg 1.7-1.9cotton 100 mg 10-25 µg 1.7-1.9

Dna yield depends on sample types


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DNAsecure Plant KitFor quick genomic Dna purification from a wide range of plant samples

Description Dnasecure Plant Kit is based on silica membrane technology and unique buffer to eliminate impurities. it is suitable for genomic Dna purification from both fresh and frozen plant samples. No phenol / chloroform extraction is needed in this kit. Dnasecure Plant Kit is ready to use and can purify the genomic Dna from a wide variety of plant species and tissues.

Features■ The procedure is fast, convenient, and can be finished within one hour.■ No phenol / chloroform extraction.■ 3-30 µg genomic DNA can be purified from 100 mg plant samples. ■ OD260 / oD280 value is within the range of 1.7 - 1.9.

ApplicationsPurified DNA using this kit is sized up to 40 kb, and suitable for downstream applications such as:

■ PCR, real-time PCR and multiplex PCR■ RAPD, RFLP, AFLP and SSR■ Library construction and Southern Blot

Required Reagents Liquid nitrogen, ethanol (96% -100%)

Typical DNA Yields





Kit Components 50 preps 200 prepsBuffer LP1 25 ml 100 mlBuffer LP2 10 ml 40 mlBuffer LP3 21 ml 84 mlBuffer Pw 15 ml 50 mlBuffer te 15 ml 60 mlRnase a (10 mg/ml) 300 µl 1.25 mlSpin columns cB3 50 200collection tubes (2 ml) 50 200Store at room temperature (15-25℃)

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genomic Dna was isolated from 100 mg leaves of differentplants using DNAsecure Plant Kit. 3 μl of 100 μl eluateswere loaded per lane.1: Fresh soy leaves2: Fresh tobacco leaves3: Fresh wheat leaves4: Fresh corn leavesm: TIANGEN λ DNA/Hind III Marker


arabidopsis thaliana 100 mg 3-4 µg 1.7-1.9wheat 100 mg 25-30 µg 1.7-1.9Pine needles 100 mg 25-30 µg 1.7-1.9Potato 100 mg 4-6 µg 1.7-1.9tomato 100 mg 10-15 µg 1.7-1.9cole 100 mg 2-4 µg 1.7-1.9tobacco 100 mg 20-25 µg 1.7-1.9Rice 100 mg 10-25 µg 1.7-1.9Soy 100 mg 20-30 µg 1.7-1.9corn 100 mg 20-30 µg 1.7-1.9

Dna yield depends on sample types. all samples above are fresh leaves.


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DNAquick Plant SystemQuick and flexible genomic DNA purification from plant samples with a wide range of starting amount

Description Dnaquick Plant System uses special buffer system for gDna extraction from a wide range of fresh and frozen plant samples. No phenol / chloroform extraction is needed. Dnaquick Plant system is available for different starting sample amounts. Dnaquick Plant system can greatly exclude protein impurities and organic compounds. The purified DNA is suitable for a wide range of downstream applications.

Features■ The fast and convenient purification process can be finished within one hour.■ No phenol / chloroform extraction and no restriction to starting sample amount. ■ High-quality kit at reasonable price, especially suitable for high-throughput plant

genomic DNA purification.

Applications■ PCR, real-time PCR and multiplex PCR■ RAPD, RFLP, AFLP and SSR■ Library construction and Southern Blot

Required Reagents Liquid nitrogen, isopropyl alcohol, 70% ethanol

Typical DNA Yields





Kit Components 50 preps 200 prepsBuffer FP1 25 ml 100 mlBuffer FP2 10 ml 40 mlRnasea (10 mg/ml) 300 µl 1.25 mlBuffer te 15 ml 60 ml


m 1 1 2 2 3 3 4 4

genomic Dna was isolated from 100 mg leaves of different plants using Dnaquick Plant System. 3 µl of 100 µl eluates were loaded per lane.m: TIANGEN λ DNA/Hind III Marker1: Fresh soy leaves2: Fresh tobacco leaves3: Fresh wheat leaves4: Fresh corn leaves


arabidopsis thaliana 100 mg 3-5 µg 1.7-1.9wheat 100 mg 25-32 µg 1.7-1.9Pine needles 100 mg 25-32 µg 1.7-1.9Potato 100 mg 5-7 µg 1.7-1.9tomato 100 mg 12-16 µg 1.7-1.9cole 100 mg 2-5 µg 1.7-1.9tobacco 100 mg 20-30 µg 1.7-1.9Rice 100 mg 12-30 µg 1.7-1.9Soy 100 mg 20-32 µg 1.7-1.9corn 100 mg 20-32 µg 1.7-1.9

Dna yield depends on sample types; all data above were gained from plant leaves.


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TIANamp Feedstuff Animal DNA KitHigh performance genomic DNA purification from feedstuff

Description tianamp Feedstuff animal Dna Kit is developed by tiangen together with Shanghai Veterinary Feed Monitoring Association(China) as the specialized product to detect animal feedstuff. the kits are available for a wide range of samples. Purified DNA is suitable for a wide range of downstream applications, including standard PcR and real-time PcR.

Features■ Specialized product developed with Feedstuff Quarantine Department.■ Suited to a wide range of feedstuff samples and animal tissues.■ High sensitivity for feedstuff samples. 500 ng - 40 µg genomic DNA can be

extracted from 50 mg feedstuff sample.

Applications■ Purified DNA is suited to standard PCR and real-time PCR

Required Reagents ethanol (96% -100%), Rnasea (optional)





Kit Components 50 preps 200 prepsBuffer ga 30 ml 2×50 mlBuffer gB 30 ml 2×50 mlBuffer gD 13 ml 52 mlBuffer Pw 15 ml 50 mlBuffer te 15 ml 60 mlProteinase K ( 20 mg/ml ) 1 ml 4×1 mlSpin columns cB3 50 200collection tubes (2 ml) 50 200Store at room temperature (15-25℃)

1 2 3 4 5

m 1 2 3 4 5 6

genomic Dna extracted from feedstuff using tianamp Feedstuff animal Dna kitSample amount: 50 mg; elution volume: 100ul; loading volume: 3ul1: empty control without any animal component2: positive feedstuff 1 containing bovine component3: positive feedstuff 2 containing bovine component4: positive feedstuff 1 containing ovine component5: positive feedstuff 2 containing ovine componentelectrophoresis on 1% agarose gel, volage 6V/cm, lasting 20min

PcR detection of bovine and ovine Dna extracted from feedstuff respectively by species specific primersm: tiangen D2000 Dna Ladder1: empty control Dna, amplified by bovine specific primers2: empty control Dna, amplified by ovine specific primers3: positive feedstuff 1 Dna, amplified by bovine specific primers4: positive feedstuff 2 Dna, amplified by bovine specific primers5: positive feedstuff 1 Dna, amplified by ovine specific primers6: positive feedstuff 2 Dna, amplified by ovine specific primerselectrophoresis on 1% agarose gel, volage 6V/cm, lasting 20 min


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DNA Extraction Kit for FoodFor quick genomic DNA purification from deep-processed food and raw food materials

Description tiangen Dna extraction Kit for Food is specially developed for Dna purification from raw materials and deep processed food samples. The Kits are suited for Dna extraction from soybean, rice, cookies, convenient noodle, bean curd, soybean milk, tomato sauce and potato crisp, etc. no phenol / chloroform extraction is needed.

Features■ Specialized product developed for Food Safety Quarantine Department.■ No phenol / chloroform extraction.■ High-quality purified DNA can be obtained within one hour. The high pure

genomic Dna can be directly used in downstream experiments.

Applications■ Processed food PCR genetically-modified detection■ Processed food real-time PCR genetically-modified detection

Required Reagents 0.1 m tris-cl (pH 8.0) (soy sauce, tomato sauce samples), isopropyl alcohol, 70% ethanol, carrier Rna (optional)




Cat. no. QuantityDP326 100 preps

Kit Components 100 prepsBuffer gmo1 50 mlBuffer gmo2 20 mlProteinase K ( 20 mg/ml ) 2 mlBuffer te 15 ml


m torfu preserved

soy sance French

dry bun popcom

beancurd slice

PcR was performed with Dna isolated from different deep processed food samples using tiangen Dna extraction Kit for Food. 5 μl of 20 μl PCR products were loaded per lane. M: tiangen 50 bp Dna Ladder.


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TIANamp Marine Animal DNA KitFor quick genomic Dna extraction from marine animal tissues

Description tianamp marine animals Dna extraction kit is specially developed for Dna purification from marine animal tissue samples. the kits are available for Dna extraction from tissues of fish, shrimp, shellfish, crab, etc. no phenol / chloroform extraction is needed. tianamp marine animal Dna Kit thoroughly eliminates protein, fat and organic compounds impurities within marine animal tissues. the purified genomic Dna can be used directly in downstream experiments such as PcR amplification, restriction endonuclease digestion, library construction and Southern Blot.

Features■ Specially developed for marine animal samples.■ No phenol / chloroform extraction.■ High-quality purified DNA can be obtained in 1 to 2 hours. The high pure

genomic Dna can be used directly in downstream applications.

Required Reagents

ethanol (96% -100%), Rnasea (optional)

ApplicationsPurified DNA is suitable for:

■

Standard PCR and real-time PCR

■

RAPD, RFLP, AFLP, SSR■

Library Construction and Southern Blot





Kit Components 50 preps 200 prepsBuffer ga 15 ml 50 mlBuffer gB 15 ml 50 mlBuffer gD 13 ml 52 mlBuffer Pw 15 ml 50 mlBuffer te 15 ml 60 mlProteinase K 1 ml 4 × 1 mltianamp Spin columns cB3 50 200 collection tubes (2 ml) 50 200 Store at room temperature (15-25℃)

m 1 2 3 4 5 6 7 8 9 10 11 12

genomic Dna extracted from Japanese Scallop using tianamp marine animal Dna KitSample amount: 20 mg of gills, 30 mg of mantellum, digestive gland, or gonad; elution volume: 100ul; loading volume: 3 μlelectrophoresis on 1% agarose gel, volage 6V/cm, lasting 20 minm: TIANGEN λ DNA/Hind III Marker

genomic Dna extracted from muscle of Shrimp using tianamp marine animal Dna KitSample amount: 30 mg; elution volume: 100 μl; loading volume: 3 μlelectrophoresis on 1% agarose gel, volage 6V/cm, lasting 20 minm: TIANGEN λ DNA/Hind III Marker

genomic Dna extracted from crucian carp using tianamp marine animal Dna Kit.Sample amount: 20 mg of gills or 30 mg of muscle; elution volume: 100 μl; loading volume: 3 μlelectrophoresis on 1% agarose gel, volage 6V/cm, lasting 20 minm: TIANGEN λ DNA/Hind III Marker

gills mantellum Digestive gland gonad

m Shrimp muscle

gills musclem 1 2 3 4 5 6


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TIANamp Yeast DNA KitFor genomic Dna extraction from yeast samples

Description tianamp Yeast Dna Kit is based on silica membrane technology and special buffer system for gDna extraction from yeast and other starting samples. genomic Dna binds to the silica-membrane in the presence of high salt, while the contaminants pass through the column. after the membrane is thoroughly washed to remove any remaining contaminants, the pure Dna is eluted from the membrane with low salt buffer. Genomic DNA purified with the TIANamp Yeast Dna Kit is suitable for a variety of downstream applications, including restriction analysis, PcR analysis, Southern Blot and library construction.

Features■ Purified genomic DNA can be extracted from yeast samples within one hour.■ no phenol / chloroform extraction.■ Purified DNA can be used directly for PCR, restriction digestion and Southern

Blot.

Applications■ Standard PCR and real-time PCR■ Library construction and southern blot

Required Reagents Sorbitol buffer, Lyticase, ethanol (96% -100%), Rnasea (optional)




Kit Components 100 prepsBuffer ga 15 mlBuffer gB 15 mlBuffer gD 13 mlBuffer Pw 15 mlBuffer te 15 mlProteinase K ( 20 mg/ml ) 1 mltianamp Spin columns cB3 50collection tubes (2 ml) 50



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TIANamp Virus DNA / RNA Kitcolumn-based technology for virus Dna / Rna extraction from plasma, serum, and cell-free materials

Description tianamp virus Dna / Rna kit provides a fast, simple, and cost-effective virus Dna/Rna miniprep method for virus Dna or Rna purification from plasma, serum and other cell-free materials. tianamp virus Dna / Rna kit is based on silica membrane technology and unique buffer system. carrier Rna in the Kit improves the binding of virus Rna to the tianamp membrane especially in the case of low-titer samples, and limits possible degradation of the virus Rna due to any residual RNase activity. Virus DNA/RNA purified with TIANamp Virus DNA / Rna Kit is ready for use. no phenol / chloroform extraction. High-quality virus Dna / Rna is eluted in a small volume of Rnase-free ddH2O. The purified DNA/Rna is ready for use in downstream applications such as PcR, Rt-PcR, real-time PcR, nest PcR, etc.

Features■ High quality virus DNA / RNA can be purified from samples of various virus

including HBV, HPV, HcV and enteroviruses within one hour.■ No phenol / chloroform extraction.■ High yield, excellent repeatability and totally free from contaminants and

inhibitors.

Applications■ Virus genotyping research■ Virus epidemiologic study■ Virus infectious diseases analysis and drug resistance analysis

Required Reagents ethanol (96% -100%)




Kit Components 100 prepsBuffer gB 15 mlBuffer Rw 15 mlProteinase K ( 20 mg/ml ) 1 mlCarrier RNA 310 μgRnase-free ddH2o (Bottled) 15 mlRnase-free ddH2o (tubular) 1 mlRnase-free Spin columns cR2 50Rnase-free collection tubes (2 ml) 50Rnase-free collection tubes (1.5 ml) 50



Viral Rna was purified from serum samples containing Hepatitis e virus in 5×106, 5×105, 5×104, and 5×103 copies/ml with tianamp Virus Dna/Rna Kit. Purified performance was analyzed by qRt-PcR detection with tiangen Quant qRt-PcR (SYBR green) Kit.


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TIANamp Stool DNA KitQuick and efficient isolation of DNA from stool

Description TIANamp Stool DNA Kit simplifies isolation of DNA from stool with a fast spin-column procedure. the combined effection of inhibiteX, a unique adsorption resin, and an optimized buffer leads to removal of PCR inhibitors. No phenol / chloroform extraction is required. The purified DNA is ready to use in sensitive downstream applications such as PcR and blotting procedures.

Features■ Purified DNA could be isolated from a variety of samples.■ Rapid isolation of ready-to-use Dna within one hour.■ inhibiteX tablets combined with spin column technology to ensure high-purity

Dna which can be used directly in sensitive downstream applications.

Important Notes■ Repeated freezing and thawing of stored samples should be avoided, since this

leads to reduced DNA size and amount.■ if precipitates formed in Buffer gSL or Buffer gB, warm buffer to 56°c until the

precipitate fully dissolve.■ all centrifugation steps should be carried out in a conventional centrifuge at room

temperature.



Picture of InhibitEX Tablets



Kit Components 100 prepsBuffer gSL 80 mlBuffer gB 15 mlBuffer gD 13 mlBuffer Pw 15 mlBuffer tB 15 mlProteinase K 1 mlinhibiteX tablets 50tianamp Spin columns cR2 50 collection tubes (2 ml) 50

Store at room temperature (15-25℃) for 12 months


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TIANamp Genomic DNA KitFor genomic Dna isolation from cells, blood and tissues

Description tianamp genomic Dna Kit provides a fast and easy silica-based format for Dna purification. the kit simplifies the purification of Dna from a wide range of sample types, including animal blood (≤ 1 ml), cultured cells, and tissues commonly encountered in life science, veterinary, and genotyping applications. most samples can be directly lysed with the provided buffer system, eliminating the need for mechanical disruption and reducing hands-on time. the kit provides purification of high-quality DNA free from protein and inhibitors. The purified DNA is suitable for PcR, restriction digestion and Southern Blot.

Features■ Silica-membrane technology ensures fast and ready-to-use high-quality Dna. ■ High Dna yield, excellent repeatability.■ this kit significantly removes contaminants and inhibitors, facilitating

downstream applications.

Applications■ PcR reaction■ Southern blot■ Dna library■ Restriction enzyme digestion

Required Reagents Red cell lysis buffer (for blood samples more than 200 µl), ethanol (96%-100%), Rnase a (optional)

Typical DNA Yields




Kit Components 50 preps 200 prepsBuffer ga 15 ml 50 mlBuffer gB 15 ml 50 mlBuffer gD 13 ml 52 mlBuffer Pw 15 ml 50 mlBuffer te 15 ml 60 mlProteinase K ( 20 mg/ml ) 1 ml 4×1 mltianamp Spin columns cB3 50 200collection tubes (2 ml) 50 200


Starting Materials Sample Amount Average Yieldsmammalian Blood 100-500 µl 3-10 µgBird and amphibian Blood 5 µl 5-10 µgcultured cells 106-107 cells 5-30 µganimal tissues 30 mg 10-30 µgmouse tail 1.2 cm (tip) 10-25 µgRat tail 0.6 cm (tip) 20-40 µg


genomic Dna was isolated from long-stored blood samples using tianamp gnomic Dna Kit. 3 μl of 100 ul eluates were loaded per lane.m: TIANGEN λ DNA/Hind III Marker

m


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TIANamp Blood DNA Kit (0.1 - 1 ml)Silica membrane based high quality Dna purification from blood samples

Description tianamp Blood Dna Kit is based on silica membrane technology and special buffer system for gDNA extraction effectively from fresh or frozen whole blood (with anticoagulants such as citrate, and eDta) the spin column made of new type silica-gel membrane in Blood Dna Kit can be easily bound by Dna specifically. PcR inhibitors such as impurities and proteins are completely removed in two efficient wash steps, leaving pure Dna to be eluted in either water or a buffer provided with the kit. genomic Dna isolated with this kit is of high-quality and serves as an excellent template for agarose gel analysis, restriction enzyme digestion, PCR analysis and blotting procedures.

Features■ The kit ensures high-quality DNA purification from fresh and frozen whole blood,

plasma, serum, buffy coat and body fluids.■ Entire purification process requires only 30 minutes of handling time.■ the kit yields high-quality ready-to-use Dna for sensitive downstream

applications.

Required Reagents ethanol (96% -100%), Rnase a (optional)

Typical DNA Yields





Kit Components 50 preps 200 prepsBuffer cL 60 ml 250 mlBuffer gS 15 ml 50 mlBuffer gB 15 ml 50 mlBuffer gD 13 ml 52 mlBuffer Pw 15 ml 50 mlBuffer tB 15 ml 60 mlProteinase K 1 ml 4 × 1 mltianamp Spin columns cB3 50 200 collection tubes (2 ml) 50 200 collection tubes (1.5 ml) 50 200


m 200 µl 300 µl 400 µl

genomic Dna extracted from blood samples of different volume using tianamp Blood Dna Kit. M: TIANGEN λ DNA/Hind iii marker

Starting Materials Sample Volume DNA Yields

mammalian Blood 100-500 µl 3-10 µg

500-1000 µl 4-30 µg

coagulated Blood 200-500 µl 1-8 µg

500-1000 µl 8-15 µgBird and amphibian Blood 5-20 µl 5-40 µg


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TIANamp Blood DNA Midi Kit (0.5-3 ml)For purification of high-purity genomic DNA from 0.5 ml-3 ml blood samples

Description TIANamp Blood DNA Midi Kit is specially designed for purification of genomic Dna from 0.5-3 ml fresh whole blood (treated with any common anticoagulant such as heparin, eDta or acid-citrate-dextrose), blood clot and white blood cells. it adopts innovative silica-based material and special buffer system for effective binding and extracting gDna from blood samples. the procedure completely removes contaminants and enzyme inhibitors making total DNA isolation fast, convenient, and reliable. genomic Dna isolated with this kit is of high-quality and serves as an excellent template for agarose gel analysis, restriction enzyme digestion, PCR analysis and blotting procedures.

Features■ High-quality DNA can be effectively purified from blood samples within 1 hour.■ Purified genomic DNA is ready-to-use for sensitive downstream applications.■ no phenol/chloroform extraction, no Licl and ethanol precipitation, no cscl

gradients centrifugation

Reagents Requiredethanol (96% -100%)

Typical DNA Yields20-60 μg from 1 ml mammalian blood




Kit Components 10 prepsBuffer ge 30 mlBuffer gD 13 mlBuffer Pw 15 mlBuffer tB 15 mlProteinase K 2 × 1 mltianamp Spin columns cB5-miDi 10 collection tubes (15 ml) 10



Genomic DNA was purified from 0.5 ml, 1 ml, 2 ml or 3 ml blood samples using TIANamp Blood DNA Midi Kit. 3 μl of 500 μl eluates were loaded per lane. M: tiangen D15000 Dna marker.

D15000 0.5 ml 1 ml 2 ml 3 ml


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TIANamp Blood DNA Maxi Kit (3-10 ml)For purification of high-purity genomic DNA from 3 ml-10 ml blood samples

Description TIANamp Blood DNA Maxi Kit is specially designed for purification of genomic Dna from 3-10 ml fresh whole blood (treated with any common anticoagulant such as heparin, eDta or acid-citrate-dextrose), blood clot and white blood cells. it adopts innovative silica-based material and special buffer system for effective binding and extracting gDna from blood samples. the procedure completely removes contaminants and enzyme inhibitors making total DNA isolation fast, convenient, and reliable. genomic Dna isolated with this kit is of high-quality and serves as an excellent template for agarose gel analysis, restriction enzyme digestion, PCR analysis and blotting procedures.

Features■ High-quality DNA can be effectively purified from blood samples within 1 hour.■ Purified genomic DNA is ready-to-use for sensitive downstream applications.■ no phenol/chloroform extraction, no Licl and ethanol precipitation, no cscl

gradient centrifugation

Reagents Requiredethanol (96% -100%)

Typical DNA Yields100-300 μg from 5 ml mammalian blood




Kit Components 50 prepsBuffer ge 140 mlBuffer gD 52 mlBuffer Pw 50 mlBuffer tB 15 mlProteinase K 5 × 1 mltianamp Spin columns cB5-maXi 10 collection tubes (50 ml) 10



Genomic DNA was purified from 3 ml, 5 ml, 8 ml and 10 ml blood samples using TIANamp Blood DNA Maxi Kit. 2 μl of 500 μl eluates were loaded per lane. M: tiangen D15000 Dna marker.

m 3ml 5ml 8ml 10ml


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RelaxGene Blood DNA System (0.1 - 20 ml)For safe and flexible genomic DNA isolation from whole blood

Description Relaxgene Blood Dna system uses simple centrifugation to purify high-quality genomic Dna from variable amounts of whole blood, buffy coat, and cultured cells. the simple purification procedure is performed in a single tube, which reduces costs and simplifies handling. no organic extraction or other toxic reagents are necessary. isopropyl alcohol precipitation is used to recover high yields of pure Dna. the purified Dna performs well in sensitive downstream applications.

Features■ the entire procedure is conducted in one tube.■ Protocol is easy to handle and no risk of sample mix-up.■ Flexible in amount of starting material (range from 0.1 - 20 ml whole blood).■ the kit yields high-quality ready-to-use Dna for downstream applications.

Required Reagents isopropyl alcohol, 70% ethanol

Typical DNA yields



Cat. no. QuantityDP319-01 50 mlDP319-02 200 ml

Kit Components 50 ml 200 mlBuffer cL 125 ml 2 × 250 mlBuffer Fg 30 ml 120 mlBuffer tB 30 ml 60 mlProteinase K 250 μl 1 mlStore at room temperature (15-25℃)

Starting Materials Sample Amount DNA Yield

mammalian Blood

300 µl 3-10 µg 1 ml 4-30 µg 5 ml 100-200 µg 20 ml 300-700 µg

coagulated Blood 200-500 µl 1-8 µg

500-1000 µl 8-15 µgBird and amphibian Blood 5-20 µl 5-40 µg


m 1 2 3 4 5 6

Genomic DNA was purified from 400 μl old blood samples using TIANGEN RelaxGene Blood DNA System. 3 μl of 100 μl eluates (in TB buffer) were loaded per lane. M: TIANGEN λ DNA / HindⅢ.


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TIANquick FFPE DNA KitQuick purification of Dna from formalin-fixed, paraffin-embedded tissues without xylene treatment

Description tianquick FFPe Dna Kit provides special lysis condition and unique deparaffinization method to extract DNA from formalin-fixation and paraffin-embedding (FFPe) samples. this kit significantly reverses the cross-linking and minimizes the impairment of DNA caused by the FFPE procedure. It also incorporates specific absorbent columns which completely avoids the use of toxic and hazardous organic reagents such as xylene and the overnight digestion with proteinase K. The purified DNA has high-integrity, high-purity and stability, ideally suited for diagnosis and academic research.

Features■ The whole procedure can be finished within one hour.■ Neither xylene nor other hazardous solvents are needed.■ High-purity Dna can be isolated without the overnight digestion with proteinase K.

Procedure




Kit Components 50 prepsBuffer gL 30 mlBuffer gP 3 mlBuffer gD 13 mlBuffer Pw 15 mlBuffer te 15 mltianamp Spin columns cR2 50collection tubes (2 ml) 50


elution

Lysis in high temperature

transfer of aqueous phase

Dna Binding

wash


genomic Dna was extracted from mouse's liver FFPe samples (5 pieces, 10 μm/piece) using tianquick FFPe Dna Kit (TIANGEN) or similar product from Supplier a (Supplier A). PcR amplification of 190 bp fragment (A), 300 bp fragment (B) and 750 bp fragment (C) were conducted using purified Dna as template. 5 μl of 20 μl PcR products were loaded per lane. M: tiangen Dna marker D2000.

190bp

300bp

750bp

m tiangen Supplier a

m tiangen Supplier a

m tiangen Supplier aC

B

A


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TIANamp FFPE DNA KitFor purification of Dna from formalin-fixed, paraffin-embedded tissues

Description tianamp FFPe Dna Kit is based on silica membrane technology and special buffer system for genomic Dna purification. this kit overcomes the inhibitory effect of formalin. It uses xylene to deparaffinization and specific buffer to release DNA from tissues. The purified DNA has high-integrity, high-purity, and stability, ideally suited for use in diagnosis and academic research.

Features■ Purification of genomic Dna from formalin-fixed, paraffin-embedded tissues,

suitable for a wide range of samples.■ Ready-to-use, high-quality Dna for downstream applications.■ Reliable and stable process, completely removes contaminants and PcR

inhibitors.

Procedure




Kit Components 50 prepsBuffer ga 15 mlBuffer gB 15 mlBuffer gD 13 mlBuffer Pw 15 mlBuffer te 15 mlProteinase K ( 20 mg/ml ) 1 mltianamp Spin columns cR2 50collection tubes (2 ml) 50


elution

Xylene treatment

Sample Lysis

Dna Binding

wash


PCR amplification results of genomic DNA extracted from mouse's liver FFPE samples (5 pieces, 10 μm/piece) using tianamp FFPe Dna Kit (T) or similar product from Supplier a (A). 5 μl of 50 μl eluates were loaded per lane. M: tiangen Dna marker D2000.

190bp 300bp 500bp 650 bp 1300 bp

m t a t a t a t a t a


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TIANamp N96 Blood DNA KitFor high-throughput isolation of genomic Dna from blood sample

Description tianamp n96 Blood Dna Kit is based on silica-membrane technology in a convenient 96-well format for high-throughput purification needs. no phenol / chloroform extraction is required. Dna binds specifically to the silica-gel membrane while contaminants pass through. PcR inhibitors such as impurities and proteins are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or the buffer provided with the kit. the purified genomic DNA from blood samples is ready-to-use in PCR and blotting procedures.

Features■ Rapid isolation of high-quality, ready-to-use genomic Dna.■ no phenol / chloroform extraction.■ Significant removal of contaminants and inhibitors, facilitating downstream

applications.


ProcedureSample Pretreatment




Cat. no. QuantityDP314-01 4 plates

Kit Components 4 platesBuffer cL 4×250 mlBuffer ga 2×50 mlBuffer gB 2×50 mlBuffer gD 2×52 mlBuffer Pw 3×50 mlBuffer tB 60 mlProteinase K ( 20 mg/ml ) 8×1 mltianap n96 Plates cB3 496-well 2.2ml Plates 12Plate Films 18


Genomic DNA extracted from 600 μl blood samples using TIANamp N96 Blood DNA Kit. 5 μl of 100 μl eluates were loaded per lane. M: λ Dna/Hind iii marker

Lysis

Binding

wash

wash

elution

m


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TIANamp Swab DNA KitSilica membrane based high-purity Dna purification from swab samples

Description tianamp Swab Dna Kit is based on silica membrane technology and special buffer system for effective genomic DNA purification. The spin column made of new type silica-gel membrane in swab Dna kit can be easily bounded by Dna specifically. genomic Dna from swab samples can be purified in 90 minutes. Purified DNA is of high quality and serves as an excellent template for agarose gel analysis, real-time PcR analysis and genotyping.

Features■ Silica membrane technology ensures simple and rapid purification of high-

quality Dna from swab.■ The purified DNA from swabs is ready-to-use in downstream applications.■ 0.5 - 3.5 µg genomic Dna can be isolated from one swab sample.





Kit Components 50 preps 200 prepsBuffer ga 30 ml 2 × 50 mlBuffer gB 30 ml 2 × 50 mlBuffer gD 13 ml 52 mlBuffer Pw 15 ml 50 mlBuffer tB 15 ml 30 mlProteinase K 1 ml 4 × 1 mltianamp Spin columns cR2 50 200 collection tubes (1.5 ml) 50 200 collection tubes (2 ml) 50 200



m 1 2 3 4 5 6 7 8 9

genomic Dna was purified from different swab samples using TIANamp Swab DNA Kit. 3 μl of 50 μl eluates (in TB buffer) were loaded per lane. M: TIANGEN λ DNA/ HindⅢ.


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TIANamp Micro DNA Kitgenomic Dna purification from small volume samples including whole blood, serum/plasma, forensic materials, blood stain and swab

Description tianamp micro Dna Kit simplifies isolation of Dna from small samples with a fast spin-column procedure. no phenol / chloroform extraction is required. PCR inhibitors such as impurities and proteins are significantly removed in two efficient wash steps, leaving pure DNA to be eluted in either water or the buffer provided with the kit. the purified genomic Dna is ready-to-use in sensitive downstream applications.

Features■ Rapid purification of high-quality DNA.■ no phenol / chloroform extraction is required.■ carrier Rna enhances binding of micro Dna to spin column membrane.■ complete removal of contaminants and inhibitors, facilitating downstream

applications.


Carrier RNAcarrier Rna supplied in tianamp micro genomic Dna kit enhances Dna binding to the tianamp spin column, especially when the target Dna is limited in the sample.




Kit Components 50 prepsBuffer ga 15 mlBuffer gB 15 mlBuffer gD 13 mlBuffer Pw 15 mlBuffer tB 15 mlProteinase K 1 mlCarrier RNA 310 μgRnase-free ddH2o 1 mltianamp Spin columns cR2 50 collection tubes (2 ml) 50



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Magnetic Genomic DNA KitMagnetic beads based quick purification of genomic DNA from blood or tissues

Description Magnetic genomic DNA kit provides quick purification of genomic DNA by using magnetic-particle technology. a wide range of sample material can be processed including tissue, whole and dried blood samples. magnetic technology provides high-quality Dna, which is suitable for direct use in downstream applications such as amplification or other enzymatic reactions. DNA binds to the silica surface of the magnetic particles and two different wash buffers are used to remove contaminants. The purified high-quality DNA can be used in demanding downstream applications and organic reagents are not required in the whole process.

Features■ Procedure is quick, simple, and easy to handle within 45 minutes.■ no phenol / chloroform extraction is required.■ the purified Dna can be used directly in PcR, restriction digestion and

hybridization procedure.■ this kit can be used on the nucleic acid automatic extraction system (open

system), such as thermo, eppendorf, tecan, Hamilton, and Beckman, etc.


Instruments Requiredmagnetic separation device




Kit Components 96 prepsBuffer gB 30 mlBuffer gD 2 ×13 mlBuffer Pw 2 ×15 mlProteinase K ( 20 mg/ml ) 2 ×1 ml maS* 2 ×1 mlBuffer tB 15 ml

*maS: magattract SuspensionStore at room temperature (15-25℃)


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Genomic DNA Individual Buffers and Accessories

Cat. no. Product Name QuantityRt122-01 Red cell Lysis Buffer 100 ml

Rt122-02 Red cell Lysis Buffer 250 ml

RT401 Lysozyme (50 mg/ml) 1 ml

RT403-01 Proteinase K (20 mg/ml) 500 μl

Rt403-02 Proteinase K (20 mg/ml) 5 ml

RT405-01 RNaseA (10 mg/ml) 200 μl

Rt405-02 Rnasea (10 mg/ml) 1 ml

RT405-11 RNaseA (100 mg/ml) 400 μl

Rt410-02 Lyticase (10 U/µl) 3000 U


Cat. no. Product Name QuantityDP302-02 tianamp Bacteria Dna Kit 50 preps

DP304-02 tianamp genomic Dna Kit 50 preps

DP304-03 tianamp genomic Dna Kit 200 preps

DP305-02 Plant genomic Dna Kit 50 preps

DP305-03 Plant genomic Dna Kit 200 preps

DP307-02 tianamp Yeast Dna Kit 50 preps

DP314-01 tianamp n96 Blood Dna Kit 4×96 preps

DP315 tianamp Virus Dna/Rna Kit 50 preps

DP316 tianamp micro Dna Kit 50 preps

DP318-02 tianamp Blood Dna Kit(0.1-1 ml) 50 preps

DP318-03 tianamp Blood Dna Kit (0.1-1 ml) 200 preps

DP319-01 Relaxgene Blood Dna System (0.1-20 ml) 50 ml

DP319-02 Relaxgene Blood Dna System (0.1-20 ml) 200 ml

DP320-02 Dnasecure Plant Dna Kit 50 preps

DP320-03 Dnasecure Plant Dna Kit 200 preps

DP321-02 Dnaquick Plant System 50 preps

DP321-03 Dnaquick Plant System 200 preps

DP322-02 tianamp Swab Dna Kit 50 preps

DP322-03 tianamp Swab Dna Kit 200 preps

DP323-02 tianamp Feedstuff animal Dna Kit 50 preps

DP323-03 tianamp Feedstuff animal Dna Kit 200 preps

DP324-02 tianamp marine animal Dna Kit 50 preps

DP324-03 tianamp marine animal Dna Kit 200 preps

DP326 Dna extraction Kit for Food 100 preps

DP329-02 magnetic genomic Dna Kit 50 preps

DP328-02 tianamp Stool Dna Kit 50 preps

DP330-02 tianquick FFPe Dna Kit 50 preps

DP331-02 tianamp FFPe Dna Kit 50 preps

DP332-01 tianamp Blood Dna midi Kit 10 preps

DP333-01 tianamp Blood Dna maxi Kit 10 preps

Kg201-01 tiancombi Dna Lyse&amp PcR Kit 50 preps

Kg201-02 tiancombi Dna Lyse&amp PcR Kit 200 preps

Overview of RNA Purification


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DP419 Rnasimple total Rna Kit

DP420 Rnaprep pure Kit (For micro Sample)

DP432 Rnaprep pure Kit (For Plant)

DP430 Rnaprep pure Kit(For cell/Bacteria)

DP439 Rnaprep pure FFPe Kit

DP433 Rnaprep pure Kit (For Blood)

DP431 Rnaprep pure Kit (For tissue) DP315/DP315-R tianamp Virus Dna/Rna Kit

Rnaprep pure Series

DP422 tianamp Dna / Rna isolation Kit

DP423 tianamp Dna/Rna/Protein isolation Kit

DP501 miRcute miRna isolation Kit

DP501 miRcute miRna isolation Kit

DP438 maggene Viral Dna / Rna Kit

DP405 tRnzol Reagent

DP421 tRnzol-a+ Reagent

DP419 Rnasimple total Rna Kit

DP437 Pnaplant plus Reagent

DP408 Rnastore Reagent

DP440 RnaLock Reagent

DP409 Rnasafe (Rnase inhibitor)

DP418 Rnasin (Rnase inhibitor)

DP412 Rnaclean Kit

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Silica-membrane technology

magnetic Particle technology

Phenol/chloroform extraction

Sample Stabilization

Rna Protection

Rna cleanup

TIANGEN RNA Purification Products



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Sele

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Rnaprep p

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Rnaprep p

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Principles and MethodsRna can be divided into messenger Rna, transfer Rna and ribosomal RNA. Purification of RNA is a series of processes of cell lysis, releasing Rna, eliminating contaminates, including protein, Dna, and other impurities, and obtaining high-purity Rna.

Purification ProcessSample preparationPurify Rna from samples of different sources (such as bacteria, yeast, blood, animal tissues, plant tissues and cultured cells), or of the same source in different forms (such as fresh blood, frozen blood, blood clots and dried blood).

Requirements for samplesFresh samples or fresh samples frozen at -80℃ immediately after collection are the most suitable sources for RNA purification. Repeated freeze-thaw circles should be avoided, which led to Rna degradation and low yields.

Sample pre-treatment methods■ Plant sample – Liquid nitrogen grinding■ animal sample –tissue homogenation, liquid nitrogen

grinding■ Bacteria sample – Lysozyme treatment■ Yeast sample – Liquid nitrogen grinding, glass beads or

lysozyme treatment

Cell lysis■ guanidine isothiocyanate / Phenol method

Phenol / chloroform extraction is a liquid / liquid extraction technique in biochemistry. it is widely used in molecular biology for Rna isolating. this method relies on phase separation by centrifugation of a mix of the aqueous sample and a solution containing water-saturated phenol, chloroform and a chaotropic denaturing solution (guanidine isothiocyanate) resulting in an upper aqueous phase and a lower organic phase (mainly chloroform). Rna are in the aqueous phase, while proteins remain in organic phase. in the next step, Rna is recovered from the aqueous phase by precipitation with isopropanol or ethanol. tRnzol (DP405), tRnzol-a+ (DP421), and Rnasimple (DP419) are RNA purification kits developed based on guanidine isothiocyanate / phenol method. tRnzol is widely used for animal tissues, microbial tissues, cultured cells and plant that are not abundant in secondary metabolites.

Introduction to RNA Purification Technology

cell lysis

Reagent extraction

extraction add spin column

Remove proteinPrecipitation

wash

wash

elution

Dissolved Rna

Purified RNA

Purified RNA

Kit extraction



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Rna abundance. it is recommended to increase starting tissue amount, and take complete grinding in liquid nitrogen.

Tissues rich in protein or fatFat content is high in brain or plant. Phenol / chloroform extraction should be done repeatedly for purifying the supernatant.

Nucleic acid / RNase rich tissuesRnase content is rich in spleen, and thymus tissues, liquid nitrogen grinding and quick homogenate can effectively inactivate Rnase. Repeated phenol / chloroform extraction can remove residual Dna. if white precipitate forms immediately after adding ethanol, that may indicate Dna contamination. this can be solved by using acid phenol / chloroform extraction or Dnase Ⅰdigestion to eliminate Dna contamination.

Plant tissues and fungal tissuesRNA purification from plant tissues and fungal tissues are much more complicated than animal tissues. Sample grinding in liquid nitrogen is often necessary to prevent Rna from degradation. if Rna degradation cannot be solved by using this method, it is most likely caused by the impurities within the samples. many plants and fungi are rich in polysaccharide and polyphenol which are difficult to be removed and may precipitate or bind with RNA. extra steps or special reagents are needed (such as tiangen DP437 Rnaplant plus) to remove polysaccharide and polyphenol contamination.

RNA Identification and Evaluationextracted Rna must take appropriate quality testing after puri f icat ion to make sure i t is suitable for downstream applications. Rna quality standards differ for various experiments. intact Rna with no inhibitory impurities is required for cDna library construction. intactness of Rna is vital for northern blot, but inhibitory impurities are more tolerated. Rt-PcR requires relatively low intactness level of Rna, but it is sensitive for inhibitory impurities. Different purification methods of RNA should be adapted for various downstream applications.

RNA yield testing ─ ultraviolet spectrophotometryRna abundance is varied in different tissues. Rna concentration can be estimated using ultraviolet spectrophotometry which detects a linear change in absorbance with concentration. Rna has its absorption maximum at 260 nm and the ratio of the absorbance at 260 and 280 nm is used to assess the Rna purity. oD value at 260 nm is used to determine the Rna concentration in a solution. an oD260 reading of 1.0 is equivalent to about 40 µg/ml of Rna. the ratio of oD260/280 of a pure Rna sample without protein contamination is between 1.8 and 2.

concentration of Rna (µg/ml) = oD260 X 40 µg/ml X dilution ratio

■ Guanidine salt / β-Mercaptoethanol methodSuitable for purifying Rna from animal tissues and plants not abundant of secondary metabolites.β-Mercaptoethanol is used as a denaturing reagent to inhibit Rnase activity and keep Rna intact from degradation. TIANGEN RNAprep pure series purification kits are based on this method for Rna isolation from various samples.

■ Other methodsSome plant sampels are rich in polysaccharide and polyphenol such as fruits and tomato leaves; some have high degree of lignification such as roots organization. Rnaplant plus (DP437) are specially developed by tiangen for these samples. it can easily extract high-quality intact total Rna from plants rich in secondary metabolites.

RNA Purification

Requirements for purity■ no inhibitory organic solutions; low concentration of salts in

samples■ no contamination of biological macromolecular such as

protein, polysaccharide, and lipids ■ no Dna molecule contamination

Purification method and precipitation

Method one: organic solvent extraction■ Phenol / chloroform extraction

Phenol / chloroform extraction is a classical method to efficiently eliminate proteins from nucleic acid samples. During extraction, Rna migrates to the aqueous phase while Dna and proteins remain in the interphase or organic phase. tiangen’s tRnzol and Rnaplant plus are based on this method.

■ method two: Silica-membrane based adsorptionwith the improvement of technology, adsorption material based products have been developed for easier purification of nucleic acid. Silicon substrate adsorption is one of the most widely used methods due to its fast and simple features and no need of toxic organic compounds such as phenol and chloroform.Rnasimple total Rna, Rnaprep pure series are based on silica-membrane adsorption technology for Rna purification. Using specific spin columns and unique buffer system, protein and organic impurities are removed and high-quality Rna can be obtained.

RNA Purification for Special TissuesFibrous tissuesEfficient cell lysis is vital for purifying RNA from heart and skeletal muscle fibrous tissues. These samples have low cell density and



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Total RNA (µg) extracted from 1 mg sample

High yields ( 4 - 2 µg ) Moderate yields ( 2 - 0.05 µg ) Low yields ( 0.05 - 0 µg )

Liver Brain Bladder

Spleen embryo Bone

Heart Kidney adipose

Lung

ovarian

thymus

RNA purity testing ─ ultraviolet spectrophotometryRatio of oD260/oD280 between 1.9 and 2.1, indicates high-purity of the Rna;Ratio of oD260/oD280 less than 1.8, indicates protein impurity;Ratio of oD260/oD280 larger than 2.2, indicates Rna degradation;Ratio of oD260/oD280 less than 2.0, indicates residual guanidine isothiocyanate and β-Mercaptoethanol.attention: oD260/oD280 values increase when buffer te is used for dissolution or elution of Rna.

RNA Integrity Identification ─ Agarose gel electrophoresis

Denaturing gel electrophoresiswe can ident i fy Rna integri ty by using denaturing gel electrophoresis; but usually conventional electrophoresis testing is also capable of doing the identification.rRna account for about 80%-85% of total Rna. 28S (23S) and 18S (16S) rRna can be seen in agarose gel. 28S rRna is about double amount of 18S rRna, which indicates the integrity of Rna. rRna sizes from different soures are in the following table.

Conventional electrophoresisas denaturing gel electrophoresis process is much more complicated in conventional electrophoresis, therefore Rna integrity testing can be done in conventional electrophoresis, total Rna bands are the same for conventional electrophoresis and denaturing gel electrophoresis.

Sample types rRna types Sizes ( Kb )

Human 18 S 1.9

28 S 5.0

mouse 18 S 1.9

28 S 4.7

Drosophila 18 S 2.0

28 S 4.1

16 S 1.5

tobacco leaves 18 S 1.9

23 S 2.9

25 S 3.7

Yeast 18 S 2.0

26 S 3.8

e.coli 16 S 1.5

23 S 2.9

Xenopus laevis 18 S 1.8

28 S 4.0

RNA ProtectionRna purification is much more complicated compared to Dna purification. it comes from the fact that Rna is very easily degradable because of the following internal and external factors.

Starting sample amount for RNA extraction

Starting samples Sample amount

animal or plant tissues 20 - 100 mg

mammal cells 1×102 - 1×106 cells or 10 cm2 culture plate

g- bacteria 107 – 109 cells or 1.8 ml culture (oD600 = 2-3)

g+ bacteria 107 – 109 cells or 1.8 ml culture (oD600 = 1.8-2)

Yeast cells 1×107 cells or 1.5 ml culture (oD600 = 1.8-2)



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internal factor: residues of nucleotide in Rna molecular have two hydroxyl groups, located at 2′ and 3′ positions respectively. The presence of these two hydroxyl groups makes Rna much more chemically reactive than Dna.

external factor: Rnase is widely existed in the surrounding environment. it cannot be easily inactivated by high temperature; therefore Rna becomes very prone to cleavage by contaminating Rnases.

RNA protection prior to extractionSample collection and storageFresh sample is more appropriate for Rna purification. we should first select the fresh sample and take quick liquid nitrogen grinding and homogenate treatment to ensure the integrity of RNA in sample. If immediately purification of RNA is not possible after sample collection, proper storage method is necessary. Storing samples in liquid nitrogen is a commonly used method to prevent Rna degradation. Repeated freeze-thaw cycles should be avoided.

RNase-free experimental conditionRnase is abundant in natural environment and surrounding air. Rna purification experiment should be conducted in a special area which is free of Rnase and prevented from cross-contamination with other experimental areas.

RNase-free consumables and reagentsSterile, disposable plasticware is generally free of Rnase contamination. glassware should be treated by baking at 150℃ for >4 hours. in addition, treatment with 0.1% solution of diethylpyrocarbonate (DePc), an inhibitor of Rnase is sometimes used prior to autoclaving and baking glassware. Hands are a major source of Rnase contamination. Use gloves at all stages of preparation. Segregate Rnase-free glassware and reagents in the lab. DePc treated H2o is also used for preparing reagents. add 1ml DePc per liter of water, incubate for 12 hours, and then autoclave for 15-20 min. Use this treated water for preparing all solutions.

RNA protection in purification processSample homogenatechoose the r ight homogenate method to minimize the homogenate time and keep a low temperature. one of the most commonly used homogenate method is liquid nitrogen grinding. During grinding, don not let the liquid nitrogen volatilize completely and keep the sample frozen always. if the sample begins to thaw, Rna will be degraded by endogenous Rnase.

Cell lysisSuitable and sufficient cell lysis buffer is important for cell lysis. the lysis buffers of tiangen’s tRnzol, Rnasimple and Rnaprep kits all have inhibitory effects on Rnase. Sample amount should be in right proportion to lysis buffer. too much sample amount led to inefficient cell disruption and compromised Rnase inhibition, which cause low Rna yields and undermine the integrity of Rna.

Storage conditionafter obtain the high-quality Rna, Rna protection is of great importance in the next stage. the most vital point is to prevent Rna from degradation. tiangen Rnasafe (DP409) provides a simple solution to this problem. Rnasafe is a unique chemical reagent which inhibits Rnase and removes Rnase contamination from solution to keep Rna intactness. it is compatible with various buffer solutions and further sterilization is no needed.

Downstream applicationsRna purif ication is the base for downstream molecular experiments, such as Rt-PcR, northern blot, in vitro transcription and translation. in these downstream applications, continued Rna protection is needed, and Rnasin (DP418) is one of the widely used Rnase inhibitor in the following experiments.

RNA Purification Products


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TRNzol ReagentFor purification of RNA from a wide range of samples

Description tRnzol Reagent is a ready-to-use reagent for the isolation of total Rna from cells and tissues. the reagent is a monophasic solution of phenol and guanidine isothiocyanate. tRnzol Reagent maintains the integrity of the Rna, during sample homogenization or lysis, while disrupting cells and dissolving cell components. addition of chloroform followed by centrifugation separates the solution into an aqueous phase and an organic phase. Rna remains exclusively in the aqueous phase. after transfer of the aqueous phase, the Rna is recovered by precipitation with isopropyl alcohol. after removal of the aqueous phase, the Dna and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields Dna from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. this technique performs well with small quantities of tissue (50-100 mg) and cells (5×106), and large quantities of tissue (>1 g) and cells (>107), of human, animal, plant, or bacterial origin. the simplicity of the tRnzol Reagent method allows simultaneous processing of a large number of samples. the entire procedure can be completed in one hour. total Rna isolated by tRnzol Reagent is free of protein and Dna contamination. it can be used for northern blot analysis, dot blot, and cDna synthesis.

Features■ tRnzol Reagent is yellow and easy to distinguish between aqueous and

organic phase.■ Purified RNA is free of genomic DNA and other contaminations.■ High yield Rna for variable kinds of applications.

Required Reagents chloroform, isopropyl alcohol, 75% ethanol (in DePc-treated water), Rnase-free ddH2o

StorageStored at 2-8℃ and in dark condition for 9 -12 months

Typical DNA Yields




m 1 2 3 4 5 6

total Rna extracted using tiangen tRnzol Reagent and similar product from Supplier a.experiment Result: total Rna extracted from 1×106 Human Jurkat cells1, 2, 3: Rna extracted using tiangen tRnzol Reagent.4, 5, 6: Rna extracted using similar product from Supplier a.m: tiangen marker iii

Starting Sample Sample Amount Yield Rangewheat 1 g 100-200 µgmouse Liver 1 mg 6-10 µgculture cells 106 5-10 µge.coli culture 1 ml overnight culture 2-10 µgwhole Blood 1 ml 3-5 µg



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TRNzol-A+ ReagentHigh sensitivity total RNA purification reagent

Description tRnzol a+ Reagent is designed by tiangen on the base of tRnzol Reagent to optimize the isolation of Rna from cells and tissues. tRnzol a+ Reagent is optimized for lysis of a variety of sample types before Rna isolation with standard homogenization methods and alcohol precipitation. this reagent performs well with small amounts of tissue (50-100 mg) and cells (5×106), and large quantities of tissue (≥1 g) and cells (≥107), of human, animal or plant. tRnzol-a+ reagent can extract Rna from virus, serum and body fluids liquid samples. The entire procedure can be finished in one hour. Total RNA isolated by tRnzol a+ reagent is free of protein and Dna contamination. it can be used for Rt-PcR, northern blot analysis, and molecular cloning.

Features■ optimized lysis format, especially suited for small amounts of tissue, liquid

samples, and virus samples.■ Higher Rna yields and sensitivity compared with tRnzol Reagent.■ Suited for a wide range of sample.

Required Reagents chloroform, isopropyl alcohol, 75% ethanol (in DePc-treated water), Rnase-free ddH2o

StorageStore at 2-8℃ and in dark condition



Cat. no. QuantityDP421 100 ml

Real-time PcR results of HcV Rna extracted using tiangen tRnzol-a+ reagent. tiangen Quant qRt-PcR (SYBR green) Kit was used in qRt-PcR assay. 10-fold serial dilutions of total HcV Rna (102~105 copies/ml) were quantified by qRT-PCR.

Total RNA was purified using TIANGEN TRNzol-A+ Reagent or similar product from supplier a.

2.5

2.0

1.5

1.0

0.5

0

oD260/oD280 oD230/oD260

tiangen Supplier a



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RNAplant plus ReagentRNA purification from secondary metabolite rich plant samples

Description Rnaplant plus reagent is designed for purifying Rna from a wide range of plant samples, especially polysaccharide and polyphenol rich plant materials (such as cotton leaves, potato tubers, white pine needles, banana, apple, chinese yam, papaya, and chlorophytum leaves). 100 ml (after adding β-mercaptoethanol) Rnaplant plus Reagent can be used to purify Rna from 100 mg plant tissue sample for 200 times and from 5 g tissue sample for 4 times.

Features■ effectively extract total Rna for secondary metabolite rich plant samples from

polysaccharide and polyphenol rich plant materials. ■ total Rna can be extracted from samples within 1.5 hours.

Applications■ Rt-PcR■ northern blot, Dot blot■ in vitro translation, poly a screening■ cloning

Required Reagents β-mercaptoethanol, 5 M NaCl, Chloroform, Isopropyl alcohol, 75% Ethanol (in DePc-treated water), Rnase-free ddH2o

StorageStore at Rt and store at 4℃ after adding β-mercaptoethanol




total Rna extracted from a variety of plant samples using Rnaplant plus reagent.experimental samples: apple fruit (0.1 g); pear fruit (0.1 g); potato tuber (0.05 g); spiderivy leaves (0.05 g).experimental procedures: extract total Rna using Rnaplant plus reagent from tiangen or trizol Reagent from Supplier a according to supplier's instruction.MIII: TIANGEN Marker III; RNA volume amount: 4 - 6 μl.

0.1 g apple fruit

0.05 g potato tuber

0.05 g spiderivy leaves

0.1 g pear fruit

miii Rnaplant plus trizol






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RNAsimple Total RNA KitHigh effective total Rna purification using silica membrane technology

Description the Rnasimple total Rna Kit uses a new total Rna isolation technology based on guanidine thiocyanate / Phenol method. this kit contains a unique buffer RZ which minimizes the contamination from genomic Dna and proteins. the kit is ready to use for the isolation of total Rna from blood, cells, tissues and plant samples. Rnase-free Spin column cR3 capacity is 50-100 mg tissues or 5×106 cells for each experiment. Total RNA can be purified in one hour using this kit. total Rna isolated by Rnasimple kit is free of protein and Dna contamination and can be used for various downstream applications.

Features■ Purified Rna is free of contaminations and ready-to-use for downstream

applications.■ High quality RNA can be purified from a wide range of samples.■ The procedure is quick, convenient and can be finished in one hour.

Required Reagents chloroform, ethanol (96% -100%)

Experimental Procedure




total Rna was extracted from 20 mg rat spleen samples using RNAsimple Total RNA Kit. 3 μl of 100 μl eluates were loaded per lane.

Contents and StorageKit Components 50 prepsBuffer RZ 60 mlBuffer RD 12 mlBuffer Rw 15 mlRnase-free ddH2o 15 mlRnase-free Spin columns cR3 50 Rnase-free collection tubes (1.5 ml) 50Rnase-free collection tubes (2 ml) 50

Buffer RZ should be stored at 2-8℃and in dark condition. other reagents can be stored at room temperature.

1 2 3 4 5 6

Lysis

isolation

wash

elution



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RNAprep pure Kit (For Plant)For purification of total RNA from plants and fungi

Description Rnaprep pure Kit (For Plant) provides a fast, simple, and cost-effective method for purification of total RNA from plant samples by using effective spin column and unique buffer system. the kit includes Rnase-free Filtration column cS for homogenizing and filtering viscous plant or fungal lysates, and spin column cR3 for purifying high-quality Rna by using silica-membrane technology. High-quality total Rna can be obtained in 30 - 40 minutes. the whole process is simple, easy to operate, low toxic, and safe. the obtained Rna has high-purity and is free from protein contamination. if sample is rich in secondary metabolism, buffer HL can be provided by tiangen to achieve maximum purification efficiency.

Features■ optimized buffers for plant samples make the process more convenience.■ Unique Dnase i minimizes genomic Dna contamination.■ Unique filtration column CS eliminates other contaminations.■ High-purity ready-to-use Rna, suited for sensitive downstream applications.■ no phenol / chloroform extraction, Licl and ethanol precipitation, and cscl

gradients centrifugation.

Required Reagents β-mercaptoethanol, ethanol (96% -100%), Buffer HL (for samples rich in secondary metabolism)

Applications■ Rt-PcR; Real-time Rt-PcR■ northern Blot, Dot Blot■ chip analysis■ in vitro translation, Poly a Screening, molecular clone

Typical RNA Yields




total Rna was isolated from 80 mg atenia cordifolia leaves using Rnaprep pure Kit (For Plant). 3 µl of 100 µl eluates were loaded per lane.

Contents and StorageKit Components 50 prepsBuffer RL 30 mlBuffer Rw1 40 mlBuffer Rw 15 mlDnase i (1500 U) 1Buffer RDD 4 mlRnase-free ddH2o (tubular) 1 mlRnase-free ddH2o (Bottled) 15 mlRnase-free Spin columns cR3 50Rnase-free Filtration columns cS 50Rnase-free collection tubes (1.5 ml) 50Rnase-free collection tubes (2 ml) 100

Dnase i, Buffer RDD and Rnase-free ddH2o (tubular) should be stored at 2-8℃. other reagents can be stored at room temperature.

1 2 3 4 5 6

Starting Sample Sample Amount Yield RangeYeast 7×107 cells 30-100 µgtobacco Leaves 100 mg 73 µgarabidopsis thaliana 100 mg 35 µgcorn Leaves 100 mg 25 µgtomato Leaves 100 mg 65 µg



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RNAprep pure Kit (For Tissue)For purification of up to 100 µg total RNA from animal tissues

Description Rnaprep pure Kit (For tissue) provides a fast, simple, and cost-effective method for purification of total RNA from animal tissues by using effective spin column and unique buffer system. the kit includes Rnase-free spin columns cR3 for purifying high-quality Rna by using silica-membrane technology. High-quality total Rna can be obtained in 40-50 minutes with high-purity and is free from protein and genomic Dna contamination.

Features■ optimized buffers for animal tissue samples make the process simple and

convenient.■ Unique Dnase i minimizes genomic Dna contamination.■ Higher-purity ready-to-use Rna, suitable for sensitive downstream applications.■ no phenol / chloroform extraction, no Licl and ethanol precipitation, no cscl


Required Reagents β-mercaptoethanol, Ethanol (96% -100%)

Applications■ Rt-PcR■ northern Blot, Dot Blot■ Real-time Rt-PcR■ chip analysis■ in vitro translation, poly a screening




Total RNA purified from different tissue samples of rat using Rnaprep pure Kit (For tissue). Sample amount: 20 mg embryo (13 days), 15 mg kidney, 10 mg liver, 15 mg spleen, 10 mg thymus, 20 mg lung; elution volume: 100 μl; loading volume: 3 μl3 μl of 100 μl eluates were loaded per lane.m: tiangen marker iii

typical Rna purification yields of different rat tissue samples using Rnaprep pure Kit (For tissue).

Contents and StorageKit Components 50 prepsBuffer RL 30 mlBuffer Rw1 40 mlBuffer Rw 15 mlProteinase K 500 μlgrinding Pestles 10Dnase i (1500 U) 1Buffer RDD 4 mlRnase-free ddH2o (tubular) 1 mlRnase-free ddH2o (Bottled) 40 mlRnase-free Spin columns cR3 50Rnase-free collection tubes (1.5 ml) 50Rnase-free collection tubes (2 ml) 50


m embry

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embryo Kidney Liver Spleen thymus Lung

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RNAprep pure Kit (For Cell / Bacteria)For purification of high-quality total RNA from cells and bacteria

Description Rnaprep pure Kit (For cell / Bacteria) provides a fast, simple, and cost-effective method for purification of total RNA from cultured cells and bacteria samples by using effective spin column and unique buffer system. the kit includes Rnase-free spin column cR3 for purifying high-quality Rna by using silica-membrane technology. High-quality total Rna can be obtained in 30-40 minutes with high-purity and is free from protein and genomic Dna contamination.

Features■ optimized buffers and protocols for cultured cells and bacteria samples make

the process simple and convenient.■ Unique Dnase i minimizes genomic Dna contamination.■ Unique Rnase-free Filtration columns cS eliminates other contaminations. ■ High-purity ready-to-use Rna, suitable for sensitive downstream applications.■ no phenol / chloroform extraction, no Licl and ethanol precipitation, no cscl



Applications■ Rt-PcR■ northern blot, Dot blot■ Real-time Rt-PcR■ chip analysis■ in vitro translation, poly a screening




total Rna was purified from human Jurkat cells (1×106) using RNAprep pure Kit (For Cell / Bacteria). 3 μl of 50 μl eluates were loaded per lane.

total Rna was purified from toP10 E.coli (1×108) using RNAprep pure Kit (For Cell / Bacteria). 3 μl of 50 μl eluates were loaded per lane.

Contents and StorageKit Components 50 prepsBuffer RL 30 mlBuffer Rw1 40 mlBuffer Rw 15 mlDnase i (1500 U) 1Buffer RDD 4 mlRnase-free ddH2o (tubular) 1 mlRnase-free ddH2o (Bottled) 15 mlRnase-free Spin columns cR3 50Rnase-free Filtration columns cS 50Rnase-free collection tubes (1.5 ml) 50Rnase-free collection tubes (2 ml) 100


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RNAprep pure Kit (For Blood)For purification of total RNA from whole blood

Description Rnaprep pure Kit (For Blood) provides a fast, simple, and cost-effective method for purification of total RNA from blood samples by using effective spin column and unique buffer system. the simplicity of the procedure allows rapid parallel processing of multi-sample total RNA purification. High-quality total RNA can be obtained within 30 - 40 minutes with highest purity and is free from protein and other contamination.

Features■ optimized buffers and protocols for blood samples make the process simple

and convenient.■ Unique Dnase i minimizes genomic Dna contamination.■ Unique Rnase-free Filtration columns cS provides Rna free from other

contaminations.■ High-purity ready-to-use Rna, suitable for sensitive downstream applications.■ no phenol / chloroform extraction, no Licl and ethanol precipitation, no cscl



Applications■ Rt-PcR■ northern blot, Dot blot■ Real-time Rt-PcR■ chip analysis■ in vitro translation, poly a screening

Ordering InformationCat. no. QuantityDP433 50 preps

Contents and StorageKit Components 50 preps10 x Red cell Lysis Buffer 60 mlBuffer RL 30 mlBuffer Rw1 40 mlBuffer Rw 15mlDnase i（1500 U） 1Buffer RDD 4 mlRnase-free ddH2o (tubular) 1 mlRnase-free ddH2o (Bottled） 15 mlRnase-free Spin columns cR2 50Rnase-free Filtration columns cS 50Rnase-free collection tubes (1.5 ml) 50Rnase-free collection tubes (2 ml) 100



total Rna was isolated from the blood of object S (S) or object L (L) with tiangen Rnaprep pure Kit (For Blood), or Rna isolation product from Supplier a (Supplier A). 500 ng of isolated Rna were loaded per lane.

Supplier a tiangenS S L L S L



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RNAprep pure Kit (For Micro Sample)For purification of high-quality total RNA from micro samples

Description Rnaprep pure Kit (For micro Sample) provides a fast, simple, and cost-effective method for purification of total RNA from micro samples by using effective spin column and unique buffer system. this kit can purify total Rna from wide range of micro samples. Rnaprep pure Kit (For micro Sample) is supplied with carrier Rna to enhance binding of nucleic acid to the spin column membrane. High-quality total Rna can be obtained in 30 - 40 minutes. compared to other Rna purification methods, RNAprep pure Micro Kit selectively removes < 200nt RNA (5.8S rRna, 5S rRna, and tRnas), and improves enrichment, isolation and purification of > 200nt RNA. The obtained RNA has high-purity and is free from protein contamination.

Features■ optimized buffers and protocols for purification high-quality Rna from micro

samples, such as microdissection tissues, fiberous tissues and cells.■ Unique Dnase i minimizes genomic Dna contamination. ■ High-purity ready-to-use Rna, suitable for sensitive downstream applications.■ no phenol / chloroform extraction, no Licl and ethanol precipitation, no cscl



Applications■ Rt-PcR, Real-time Rt-PcR■ northern blot, Dot blot■ chip analysis■ in vitro translation, poly a screening


Contents and StorageKit Components 50 prepsBuffer RL 30 mlBuffer Rw1 40 mlBuffer Rw 15 mlcarrier Rna 310 μgDnase i (1500 U) 1Buffer RDD 4 mlRnase-free ddH2o (tubular) 1 mlRnase-free ddH2o (Bottled) 2 × 15 mlRnase-free Spin columns cR1 50Rnase-free collection tubes (1.5 ml) 50Rnase-free collection tubes (2 ml) 50


Result of qRt-PcR from purified Rna using Quant qRt-PCR (SYBRGreen I) Kit (FP302). Total RNA purified from Hela cells (1×106, 1×105, 1×104, 1×103, 1×102, 10) cells using Rnaprep pure Kit (For micro Sample) of tiangen.


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RNAprep pure FFPE KitFor purification of Rna from formalin-fixed, paraffin-embedded tissues

Description the Rnaprep pure FFPe Kit is specially designed for purifying total Rna from formalin-fixed, paraffin-embedded tissue sections. Special lysis and incubation conditions reverse formaldehye modification of Rna. in addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. the kit also uses Dnase Ⅰand Buffer RDD for optimized removal of genomic Dna contamination. the purified Rna can be used in various of downstream applications

Features■ Silica membrane based technology to ensure high-purity and integrity of Rna.■ Fast and simple process compared with conventional methods.■ Suitable for downstream Rt-PcR or Rt-qPcR applications.

Required Reagents Xylene ethanol (96% -100%)


Contents and StorageKit Components 50 prepsBuffer RF 12 mlBuffer RB 12 mlBuffer Rw1 40 mlBuffer Rw 15 ml Proteinase K 500 lRnase-free ddH2o (Bottled) 40 mlRnase-free Spin columns cR3 50Dnase i (1500 U) 1Buffer RDD 4 mlRnase-free ddH2o (tubular) 1 mlRnase-free collection tubes (1.5 ml) 50Rnase-free collection tubes (2 ml) 50


Rna extracted from paraffin-embedded rat liver sample using Rnaprep pure FFPe KitSample amount: 15 mg rat liver; elution volume: 50 μl; loading volume: 8 μlm: tiangen marker Ⅲ


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TIANamp Virus DNA/RNA KitFor virus DNA or RNA purification from plasma, serum or cell-free materials

Description tianamp Virus Dna / Rna Kit provides a fast, simple, and cost-effective method for virus DNA or RNA purification from whole blood, plasma, serum and other cell-free materials by using effective spin column and unique buffer system. tianamp Virus Dna / Rna Kit is supplied with carrier Rna to enhance binding of nucleic acid to the spin column membrane. the obtained genomic Dna or Rna is free from protein and nuclease contamination. it can be used directly in PcR, restriction endonuclease digestion, hybridization, and reverse transcription experiments. tianamp Virus Dna / Rna Kit is used for wide range of virus Dna / Rna purification such as HcV, HiV, HPV, and animal pathogenic virus. the high-quality Dna or Rna can be obtained within one hour.

Features■ High-quality genomic Dna or virus Rna can be obtained in one hour.■ no phenol / chloroform extraction and ethanol precipitation.■ High yield and excellent repeatability.■ complete removal of contaminants and inhibitors, facilitating downstream

applications.

Applications ■ Virus genotyping research■ Virus epidemiologic study■ Virus infectious diseases analysis


Contents and StorageKit Components 50 prepsBuffer gB 15 mlBuffer Rw 15 mlRnase-free ddH2o (Bottled) 15 mlProteinase K 1 mlcarrier Rna 310 μgRnase-free ddH2o (tubular) 1 mlRnase-free Spin columns cR2 50 Rnase-free collection tubes (2 ml) 50 Rnase-free collection tubes (1.5 ml) 50



Viral Rna was purified from serum samples containing Hepatitis e virus in 5×106, 5×105, 5×104, and 5×103 copies/ml with tianamp Virus Dna/Rna Kit. Purified performance was analyzed by qRt-PcR detection with tiangen Quant qRt-PcR (SYBR green) Kit.



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TIANamp Virus RNA KitProfessional virus RNA purification kit

Description tianamp Virus Rna Kit provides a fast, simple, and cost-effective virus Rna purification method for virus RNA purification from whole blood, plasma, serum and other cell-free materials by using effective spin column and unique buffer system. this kit is supplied with carrier Rna to enhance binding of nucleic acid to the spin column membrane. the obtained Rna is free from protein and nuclease contamination and can be used directly in PcR, restriction endonuclease digestion, hybridization, and reverse transcription experiments. tianamp Virus RNA Kit is suitable for various kinds of virus RNA purification and the high-quality Rna can be obtained within one hour.

Features■ High-quality virus Rna can be obtained within one hour.■ no phenol / chloroform extraction and ethanol precipitation.■ High Rna yield and excellent repeatability.■ complete removal of contaminants and inhibitors, facilitating downstream

applications.


Applications■ Virus genotyping research■ Virus epidemiologic study■ Virus infectious diseases analysis

Ordering InformationCat. no. QuantityDP315-R 50 preps

Contents and StorageKit Components 50 prepsBuffer RL 30 mlBuffer gD 13 mlBuffer Rw 15 mlcarrier Rna 310 μgRnase-free ddH2o (tubular) 1 mlRnase-free ddH2o (Bottled) 15 mlRnase-free Spin columns cR2 50 Rnase-free collection tubes (2 ml) 50 Rnase-free collection tubes (1.5 ml) 50



Sample Duplicate ct value average

1 duplicate 1 19.71 19.70

duplicate 2 19.68

2 duplicate 1 19.8 19.78

duplicate 2 19.75

3 duplicate 1 19.49 19.53

duplicate 2 19.56 ntc none -average 19.67

Sample Duplicate ct value average 1 duplicate 1 26.44 26.40 duplicate 2 26.35 2 duplicate 1 26.37 26.36 duplicate 2 26.35 3 duplicate 1 26.33 26.35 duplicate 2 26.37 ntc none - average 26.37

Genomic DNA was purified from 200 μl of Avian influenza virus standard antigen that was diluted 100 times (A), or 10,000 times (B) with tianamp Virus RNA Kit. 4 μl of 50 μl eluates were used as template in real-time PcR detection. Real-time PcR were in triplicate for each sample.

A

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MagGene Viral DNA/RNA KitFor virus Dna / Rna purification from blood, plasma, and serum, especially suitable for incorporation with high-throughput workstation

Description maggene Viral Dna/Rna Kit is designed for rapid and reliable purification of

virus Dna / Rna from serum, plasma etc. nucleic acids (Dna / Rna) from a complex with magnetic beads in a specially formulated buffer. the beads / nucleic acids complex is then separated from lysates using a magnet. Purified Dna / Rna are then eluted when the buffer condition is adjusted. Special magnetic beads technology enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities.

Features■ Rapid purification of high quality, ready-to-use virus Dna and Rna within 45

minutes.■ no phenol / chloroform extraction.■ this system can be easily adapted with automated high-throughput nucleic acid

purification systems such as Eppendorf, Thermo, and Beckman, etc.

Applications■ Rt-PcR, Real-time Rt-PcR


Contents and StorageKit Components 50 prepsBuffer gB 30 mlBuffer Rw 3×15 mlProteinase K 2×1 mlcarrier Rna 2 ×310 μgBead Suspension Buffer g 2×1 mlRnase-free ddH2o 15 ml

transport and store at room temperature (15-25℃)

qRT-PCR result of purified RNA.Samples: total Rna extracted from Bird Flu Standard antigen (gradient dilute by 50, 100, 200, 400, 800, 1600, 3200, 6400, 12800).Primers & Probes: Bird Flu Virus specific primers and probes.PCR volume: 20 μlTemplate: 5 μl




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TIANamp DNA / RNA Isolation KitFor simultaneous purification of DNA and RNA from one sample

Description tianamp Dna / Rna isolation Kits are designed for extracting both genomic Dna and total Rna simultaneous from the same animal cells or tissue sample. this kit is compatible with a wide range of animal cells and tissues. the simple process allows the purification of high-quality Dna and Rna from the same sample within 40-50 min. The purified DNA and RNA are eluted separately and ready-to-use in sensitive downstream applications.

Features■ Simultaneous purification of high-quality DNA and RNA from one sample.■ Whole process can be finished within 40 - 50 minutes. ■ no phenol / chloroform extraction.■ Ready-to-use Dna and Rna for sensitive downstream analysis.

Required Reagents β-mercaptoethanol, ethanol (96% -100%)

Applications■ Rt-PcR, Real-time Rt-PcR


Contents and StorageKit Components 50 prepsBuffer RL plus 30 mlBuffer Rw1 40 mlBuffer Rw 15 mlRnase-free ddH2o (Bottled) 15 mlRnase-free Spin columns cR3 50Rnase-free Spin columns cB3 50Buffer gD 13 mlBuffer Pw 15 mlBuffer tB 15 mlRnase-free microcentrifuge tubes (1.5 ml) 100Rnase-free microcentrifuge tubes (2 ml) 50Rnase-free collection tubes (2 ml) 100

Store dry at room temperature (15-25℃)



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TIANamp DNA / RNA / ProteinIsolation KitFor simultaneous purification of Dna, Rna, and protein from one sample

Description DNA / RNA / Protein Isolation Kits provide simultaneous purification of genomic Dna, total Rna, and total protein from the culture animal cells and tissue sample. this kit provides multi-sample parallel processing and the whole process can be finished within one hour. There is no need to split the sample into three parts prior to purification. In addition, no organic solvents such as phenol and acetone are required.

Features■ Simultaneous purification of high-quality genomic DNA, total RNA and protein

from the same sample.■ this kit can provide parallel processing of multiple samples within one hour.■ no organic extraction and alcohol precipitation.■ Ready-to-use Dna and Rna for downstream analysis.

Required Reagents β-mercaptoethanol, ethanol (96% -100%)

Applications■ Purified genomic DNA and total RNA is suitable for PCR, RT-PCR and real-time

PcR■ Purified total protein is suited for SDS-PAGE and western blot


Contents and StorageKit Components 50 prepsBuffer RL 30 mlBuffer Rw1 40 mlBuffer Rw 15 mlBuffer PR 160 mlBuffer SP 15 mlRnase-free ddH2o (Bottled) 15 mlRnase-free Spin columns cR3 50Rnase-free Spin columns cB3 50Buffer gD 13 mlBuffer Pw 15 mlBuffer tB 15 mlRnase-free microcentrifuge tubes (1.5 ml) 100Rnase-free microcentrifuge tubes (2 ml) 50Rnase-free collection tubes (2 ml) 100




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RNAclean KitFor Rna cleanup and concentration

Description the Rnaclean Kit enables cleanup and concentration of Rna from samples using specialized Rnase-free spin columns cR2 based on silica-membrane technology. Up to 20 μg RNA sample can be treated and the obtained RNA is free from protein, salts and organic impurities. this kit enables recovery of Rna from products of enzymatic reactions (Dnase treated, Protease treated, Rna marked) and other samples. the obtained Rna can be used in northern blot, dot blot, mRna extraction, cDna synthesis, and primer extension etc.

Procedure


Contents and StorageKit Components 50 prepsBuffer RK 10 mlBuffer Rw 15 mlRnase-free ddH2o 15 ml Rnase-free Spin columns cR2 20Rnase-free collection tubes (1.5 ml) 20Rnase-free collection tubes (2 ml) 20


add buffer RK and ethanol

Rna binding

wash

elution

Obtained purified RNA



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RNAstore Reagentnon-frozen reagent for preservation of intact Rna in samples

Description Rnastore is a liquid and non-toxic tissue conservation reagent. this innovative, aqueous reagent quickly permeates tissues, stabilize and protect the Rna expression pattern, and facilitate tissue gene expression profiles analysis. tissues must be immersed in Rnastore immediately after collection for preservation. this can keep Rna intact and there is no need of immediate sample handling or preservation in liquid nitrogen. Rnastore can be used to preserve various vertebrate tissue samples such as brain, heart, kidney, spleen, liver, lung, and thymus.

Features■ the reagent preserves Rna in tissues for up to 1 day at 37℃, 7 days at 18-

25℃, or 4 weeks at 2-8℃; tissue can be stored for a long time at -20 or -80℃ after submerging overnight at 4℃.

■ Rnastore-treated tissues can be frozen and thawed repeatedly for 20 times without affecting Rna yield.

■ total Rna can be extracted directly from tissues stored in Rnastore using tiangen tRnzol Reagent, tRnzol-a+ reagent, Rnaprep pure and Rnasimple kits.

Storage Store dry at room temperature (15-25℃) for more than one year. Precipitates or crystal may form in low-temperature during storage, Dissolve completely before usage.

Points for Attention■ Rnastore can be used for storage of fresh tissue samples.■ Rnastore cannot be used for storage of plant samples.■ Usage amount of Rnastore should be at least tenfold of tissue sample amount.■ to ensure rapid and reliable stabilization of Rna even in the inner parts of solid

tissues, the sample must be cut into slices with thickness less than 0.5 cm.

Ordering InformationCat. no. QuantityDP408-01 20 mlDP408-02 100 ml

total Rna was isolated using tiangen tRnzol Reagent from 15 mg rat lung samples stored in Rnastore Reagent under the indicated conditions or stored directly at -80℃

after collection without RNAstore treatment. 3 μl of 100 μl isolation product were loaded per lane.3-4: Rna isolated from tissues stored in Rnastore at 37℃ for one day5-6: Rna isolated from tissues stored in Rnastore at Rt for one week 7-8: Rna isolated from tissues stored in Rnastore at 4℃ for four weeks c: Rna isolated from tissues directly stored at -80℃ without Rnastore treatment

total Rna was isolated from Rnastore-treated rat lung tissues subjected to 5, 10, 15 or 20 freeze/thaw cycles (as indicated). 3 μl of 100 μl isolation product were loaded per lane. C: Rna isolated from tissues directly stored at -80℃ without repeated freezing and thawing.


1 2 3 4 5 6 7 8

c 1 day 37℃ 1 week Rt 4 week 4℃

c 5 10 15 20



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RNALock ReagentFor efficient preservation of RNA in blood samples

Description RnaLock Reagent is a liquid non-toxic blood preservation reagent which can stabilize Rna in fresh blood. Human blood sample with RnaLock reagent can be stored for 5 days at 2-8℃, or 3 months at -20℃. mammal blood sample with RnaLock reagent can be stored for 2 days at 15-25℃, 7 days at 2-8℃, or 6 months at -20℃ without any detectable Rna degradation. coordinate with Rnaprep pure kit (For blood) to optimize Rna purification process, Rnalock Reagent facilitates rapid total Rna purification.

Features■ efficient protection of Rna in fresh blood to prevent degradation. ■ Suited for large amount of blood sample preservation in two steps. ■ excellent compatibility with tiangen Rna or Dna purification kit to ensure

high yield and purity.

Storage Store dry at room temperature (15-25℃)

Ordering InformationCat. no. QuantityDP440-01 20 mlDP440-02 100 ml

Rna purification from RnaLock reagent stored blood samples by using Rnaprep pure kit (for blood). m: tiangen marker Ⅲ1-2: RNA purified from fresh blood.3-4: Stored at room temperature for 2 days.5-6: Stored at 4℃ for a week.7-8: Stored at -20℃ for six months.


m 1 2 3 4 5 6 7 8

control 15-25℃ 4℃ -20℃



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RNAsafe (RNase Inhibitor)Efficient RNase inhibitor for preservation of RNA samples

Description Rnasafe reagent is a highly active Rnase inhibition. it thoroughly removes Rnase contamination to preserve the intactness of Rna.

Features■ complete removal of Rnase.■ treated solution could be retreated (60℃, 10-20 min) to further remove any

trace Rnase contaminations.■ no inhibition of Rtase, Dna polymerase, and Rna polymerase activity after

Rnasafe treatment.■ no need of sterilization after Rnasafe treatment.

Storage Store at -20℃

Applications■ Preservation, isolation, and purification of RNA ■ inhibition of susceptible Rnase in Rna samples

RNasin (RNase Inhibitor)For stabilization of purified RNA in downstream applications

Description Rnasin is a special Rnase inhibitor existing in human placenta. it is essentially a protein with molecular weight of 51,000 Da and isoelectric point of pH 4.7. Rnasin binds noncovalently to Rnase and inhibits its activity. Rnasin can keep mRna intact to enhance transcription and translation efficiency, and to avoid negative influence of organic compound inhibitors.

Features■ inhibition of eukaryotes Rnasea, RnaseB, Rnasec and human placenta

Rnase activity.■ no inhibition of Rnase H, S1 nuclease, SP6, t7, t3 Rna Polymerase, mV or

m-mLV Reverse transcriptase, taq Dna Polymerase, Rnase t1. no influence upon the transcription and translation processes.

■ 1 mm Dtt excised in the buffer system, wide pH activity ranges.

Storage Store at -20℃ and store at -70℃ for long-term preservation



Cat. no. QuantityDP409 1 ml

Cat. no. QuantityDP418 30 µl



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miRcute miRNA Isolation KitFast Purification of 20-200 nt miRNA from variable Samples

Description miRcute miRNA Isolation Kit is uniquely designed to purify cellular small (20-200 nt) RNA molecules, including miRNA, siRNA and snRNA. The Kit can also be used to purify total RNA from samples. The Kit utilizes a silica-based two-column system to enrich small RNA from various sample sources. From sample lysate, RNAs greater than ~ 200 nt are bound to the first column. The flow-through, enriched with small RNA molecules, is mixed with a higher concentration of ethanol to increase the binding affinity of small RNA to the membranes of the second column. Bound small RNA molecules are then eluted, ready-to-use, and free of large RNA molecules that can inhibit expression analysis of small RNA molecules, Purified RNA is suitable for different downtown applications.

Features■ efficiency: efficient enrichment of micro Rna and Rnas < 200 nucleotides■ convenience: effective purification of micro Rna and total Rna■ Simplity: the procedure is quick and convenient in about 1 hour.■ High Performance: High-purity Rna suitable for all downstream applications

Required Reagents Chloroform, Ethanol (96% -100%)


qRT-PCR analysis of purified miRNA extracted by DP501.Sample: miRNA extracted from 30 mg rat liver.

Purification Comparison of miRNASample: miRNA extracted from 30 mg rat liver A: Extracted by DP501B: Extracted by traditional isopropyl alcohol precipitation


Contents and StorageKit Components 50 prepsBuffer mZ 60 mlBuffer Rw 15 mlBuffer mRD 12 mlRnase-free ddH2o 15 mlRnase-free Spin columns miRspin 50Rnase-free Spin columns miRelute 50Rnase-free eP tubes (1.5 ml) 50Rnase-free collection tubes (2 ml) 50

Buffer mZ should be stored dry at 2-8℃; other Reagents at room temperature.

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RNA Purification Individual Buffers and Accessories

Cat. no. Product Name QuantityDP315 tianamp Virus Dna / Rna Kit 50 preps

DP315-R tianamp Virus Rna Kit 50 preps

DP405-01 tRnzol Reagent 20 ml

DP405-02 tRnzol Reagent 100 ml

DP408-01 Rnastore Reagent 20 ml

DP408-02 Rnastore Reagent 100 ml

DP409 Rnasafe (Rnase inhibitor) 1 ml

DP412 Rnaclean Kit 20 preps

DP418 Rnasin (Rnase inhibitor) 30 µl

DP419 Rnasimple total Rna Kit 50 preps

DP420 Rnaprep pure Kit (For micro Sample) 50 preps

DP421 tRnzol-a+ Reagent 100 ml

DP422 tianprep Dna-Rna isolation Kit 50 preps

DP423 tianprep Dna-Rna-Protein isolation Kit 50 preps

DP430 Rnaprep pure Kit (For cell/Bacteria) 50 preps

DP431 Rnaprep pure Kit (For tissue) 50 preps

DP432 Rnaprep pure Kit (For Plant) 50 preps

DP433 Rnaprep pure Kit (For Blood) 50 preps

DP439 Rnaprep pure FFPe Kit 50 preps

DP440-01 RnaLock Reagent 20 ml

DP440-02 RnaLock Reagent 100 ml

DP501 miRcute miRna isolation Kit 50 preps

Cat. no. Product Name QuantityRK123 Buffer Rw 15 ml

RK124 Filtration column cS 1

RK130 Spin column cR1 1

RK131 Spin column cR2 1

RK132 tianamp Spin column cR3 1

RK143 Buffer BL 30 ml

RK145 Buffer RZ 60 ml

RK146 Buffer Rw1 40 ml

Rt121-01 Dnase/Rnase-Free ddH2o 5×5 ml

Rt121-02 Dnase/Rnase-Free ddH2o 100 ml

Rt202 5×Rna electrophoretic Loading Buffer 5 ml

Rt401 Lysozyme (50 mg/ml) 1 ml

Rt403-01 Proteinase K (20 mg/ml) 500 µl

Rt403-02 Proteinase K (20 mg/ml) 5 ml

RT410-02 Lyticase (10 U/μl) 3000 U

Rt411 Dnasei (Rnase-free) 1500 U

Rt416-02 carrier Rna 310 µg




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96-well KitsDP115 tianprep Rapid n96 Plasmid Kit

DP114 tianprep Highpure n96 Plasmid Kit

DP104 tianpure mini Plasmid Kit

DP103 tianprep mini Plasmid KitSmall-Scale Plasmid Kits

DP107 tianpure midi Plasmid Kit

DP106 tianprep midi Plasmid Kitmedium-Scale Plasmid Kits

DP116 HighPure maxi Plasmid Kit

DP117 endoFree maxi Plasmid KitLarge-Scale Plasmid Kits

DP112 tianprep Yeast Plasmid Dna Kit

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Plasmid DNA Purification Products

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TIANprep Mini Plasmid KitHigh-performance plasmid purification using novel silica-membrane technology

Description The TIANprep Mini Plasmid Kit provides a fast, simple and cost-effective plasmid purification method for routine molecular biology laboratory applications. TIANprep Mini Plasmid Kit uses silica-membrane technology and unique buffer system to reduce contamination of protein and other organic compounds. this kit is designed to isolate plasmid DNA from 1-5 ml bacterial overnight culture, yielding up to 20 μg of plasmid DNA. The purified plasmid DNA can be used directly in downstream applications, including restriction endonuclease digestion, transformation, sequencing and PCR.

Features■ Simple and fast procedure with excellent performance.■ Efficiently extract > 85% plasmid DNA from bacterial cultures.

Ordering InformationCat. no. QuantityDP103-02 50 prepsDP103-03 200 preps

Contents and StorageKit Components 50 preps 200 prepsRNaseA (10 mg/ml) 150 μl 600 μlBuffer BL 30 ml 120 mlBuffer P1 15 ml 60 mlBuffer P2 15 ml 60 mlBuffer P3 20 ml 80 mlBuffer PD 30 ml 120 mlBuffer Pw 15 ml 50 mlBuffer eB 15 ml 30 mlSpin columns cP3 50 200Collection Tubes (2 ml) 50 200

Stored at room temperature (15-25℃)


pBS-T was purified from 1ml, 3 ml or 5 ml E.coli overnight cultures with TIANGEN TIANpure Mini Plasmid Kit. 3 μl of 200 μl eluates were loaded per lane. M: tiangen Dna ladder D15000.

m 1ml 1ml 3ml 3ml 5ml 5ml



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TIANpure Mini Plasmid KitFor purification of high-purity molecular biology grade plasmid DNA

Description The TIANpure Mini Plasmid Kit is a ready-to-use kit providing all necessary components for purification of high-quality plasmid DNA. The kit is designed for isolating up to 20 µg high-purity plasmid DNA, especially suited for nuclease rich host strains. The purified plasmid DNA can be used directly in high-precision molecular biological experiments such as transfection, etc.

Features■ Simple and fast procedure with excellent performance.■ Efficiently extract > 85% plasmid DNA from bacterial cultures. ■ Purified plasmid DNA can be used directly in high-precision experiment

including transfection.

Ordering InformationCat. no. QuantityDP104-02 50 preps

Contents and StorageKit Components 50 prepsRnase a (10 mg/ml) 150 µlBuffer BL 30 mlBuffer P1 15 mlBuffer P2 15 mlBuffer P3 20 mlBuffer PD 30 mlBuffer Pw 15 mlBuffer tB 15 mlFiltration columns cS 50Spin columns cP3 50Collection Tubes (2 ml) 100


TIANprep Midi Plasmid KitFast method for purification of up to 40 µg plasmid DNA

Description The TIANprep Midi Plasmid Kit is based on silica membrane technology to eliminate contamination of protein and other organic compounds. this kit is designed to isolate plasmid DNA from 5-15 ml overnight bacterial culture, yielding up to 40 μg of plasmid DNA. The purified plasmid DNA can be used directly in downstream applications, such as restriction endonuclease digestion, transformation, sequencing and PCR.

Features■ Simple and fast procedure with excellent performance.■ Efficiently extract > 85% plasmid DNA from bacterial cultures. ■ Up to 40 µg plasmid DNA can be purified from 5-15 ml bacterial culture.


Contents and StorageKit Components 50 prepsRNaseA (10 mg/ml) 300 μlBuffer BL 30 mlBuffer P1 30 mlBuffer P2 30 mlBuffer P3 40 mlBuffer PD 30mlBuffer Pw 15 mlBuffer eB 15 mlSpin columns cP4 50Collection Tubes ( 2 ml ) 50


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TIANpure Midi Plasmid KitFast method for purification of up to 40 µg high-purity plasmid DNA

Description The TIANpure Midi Plasmid Kit provides a uniquely developed filtration column CS based on the silica membrane technology, to ensure removal of trace protein and other impurities. this kit is designed for preparation of up to 40 µg high-purity plasmid. Purified plasmid is suited for high-precision large-scale animal cells molecular biological experiments.

Features■ Simple and fast procedure with excellent performance.■ Efficiently extract > 85% plasmid DNA from bacterial cultures. ■ Up to 40 µg plasmid DNA can be purified from 5-15 ml bacterial culture.■ Purified plasmid DNA can be used directly in downstream high-precision

molecular biological experiments.


Contents and StorageKit Components 50 prepsRnase a (10 mg/ml) 300 µlBuffer BL 30 mlBuffer P1 30 mlBuffer P2 30 mlBuffer P3 40 mlBuffer PD 30 mlBuffer Pw 15 mlBuffer tB 15 mlFiltration columns cS 50Spin columns cP4 50Collection Tubes (2 ml) 100


High-copy plasmid (pBS) or low-copy plasmid (pBR322) were purified from 5 ml or 15 ml bacterial overnight cultures with TIANGEN TIANpure Midi Plasmid Kit. 3 μl of 200 μl eluates were loaded per lane. M: tiangen marker iV.

Experiment ExamplepBR322 pBS

m 5ml 15ml 5ml 15ml



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HighPure Maxi Plasmid KitFor fast purification of up to 1.5 mg transfection-grade plasmid DNA

Description HighPure Maxi Plasmid Kit provides unique buffer system and specially developed TIANRed colour indicator which offers visual identification to ensure highest extraction efficiency of high-quality plasmid. This kit uses isopropyl alcohol precipitation method to obtain maximum amount of plasmid DNA. This kit allows flexible starting amount of bacterial overnight cultures to meet the requirements of various downstream applications.

Features■ Isopropyl alcohol precipitation method can obtain up to 1.5 mg plasmid DNA.■ Specially designed buffer P4 and filtration columns CS1 treatment ensure the

transfection-grade purity.■ Simple and fast process that can be finished within 1 hour.■ Unique colour indicator ensures the highest extraction efficiency.

Visualization Using TIANRed



Typical DNA yields


Kit Components 50 prepsRnase a (100 mg/ml) 500 µlBuffer P1 100 mlBuffer P2 100 mlBuffer P4 100 mltianRed 500 µlFiltration columns cS1 10Buffer tB 30 ml


1: Upon addition of P1 buffer with TIANRed the cell suspension turns red allowing visualization of sufficient mixing.

2: Correct mixing after addition of buffer P2 causes tianRed to turn clear purple. a homogeneous solution with traces of red indicates insufficient lysis.

3: insufficient mixing after addition of neutralization buffer P4.

4 : co r rec t m ix i ng a f t e r add i t i on o f neutralization buffer P4 causes TIANred to turn clear yellow.

Plasmid Type Sample Amount Average YieldsHigh-copy Plasmid 100 ml 500 µg – 1.5 mgLow-copy Plasmid 200 ml 200 µg – 400 µg

pegFP and pcmV plasmid Dna extracted from 100 ml bacterial culture (pEGFP: OD600=1.95; pcmV: oD600=2.05) using HighPure maxi Plasmid Kit.

pegFP pcmV

pegFP pcmV

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200

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■Yields (µg)

■ oD260 / oD280 ■ oD220 / oD260

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EndoFree Maxi Plasmid KitFor purification of endotoxin-free transfection grade plasmid DNA

Description EndoFree Maxi Plasmid Kit is based on silica membrane technology to extract endotoxin-free plasmid DNA. This kit provides a unique endotoxin removal process to generate high-quality plasmid DNA for use in demanding downstream applications. it is designed to isolate plasmid Dna of the highest purity from 100 - 200 ml of bacterial culture and can yield up to 1.5 mg transfection-grade plasmid Dna.

Features■ 200 µg -1.5 mg plasmid Dna yield in one hour with high proportion of

supercoiled structures.■ Low endotoxin level and high purity of plasmid DNA are ensured by unique

buffer system and CP6 spin columns.■ Excellent transfection efficiency, suitable for transfection experiment of most cell

lines.■ Wide range of applications, suited for restriction endonuclease digestion,

transformation, sequencing, gene therapy, cell microscopy, gene silencing and transfection experiments.



Typical DNA yields


Kit Components 50 prepsRNaseA (100 mg/ml) 500 μlBuffer BL 30 mlBuffer P1 100 mlBuffer P2 100 mlBuffer P4 100 mlBuffer Pw 70 mlBuffer tB 30 ml Filtration columns cS1 10Spin columns cP6 10Collection Tubes (50 ml) 20


Plasmid Type Sample Amount Average YieldsHigh-copy Plasmid 100 ml 500 µg – 1.5 mgLow-copy Plasmid 200 ml 200 µg – 600 µg

Low-copy plasmid pBR322, extracted from 200 ml bacterial cultures using EndoFree Maxi Plasmid Kit, was eluted in 1 ml Buffer TB, with a concentration of 0.6 μg/μl. 2 ul plasmid was loaded per lane onto the agarose gel.

High-copy plasmid pBS, extracted from 100 ml bacterial cultures using EndoFree Maxi Plasmid Kit, was eluted in 1 ml Buffer TB, with a concentration of 1.2 μg/μl. 2 μl plasmid was loaded per lane onto the agarose gel.

miV: tiangen Dna marker iV

pEGFP preparations obtained with EndoFree Maxi Plasmid Kit were each transfected into 293t (A) and Huh7 (B) cells using tiangen Dnafectin transfection Reagent. expression of gFP was detected 24 hours post-transfection.

miV pBR322 pBS miV

BA



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TIANprep Highpure N96 Plasmid KitFor purification of high-throughput transfection-grade plasmid DNA with real-time lysis monitoring

Description tianprep HighPure n96 Plasmid Kit is designed for rapid isolation of 5 -10 µg high-purity plasmid DNA from up to 1.3 ml bacterial culture each well for usage in routine molecular biology applications. This kit provides unique type of silica membrane technology to remove protein and impurities, ensuring high-purity of the isolated plasmid. The process is performed entirely in 96-well format, reducing sample handling to a minimum. The purified plasmid DNA can be used in restriction endonuclease digestion, sequencing, screening, library construction and transformation.

Features■ Designed to isolate high-purity plasmid in short time.■ Compatible with high-throughput nucleic acid purification workstations. ■ Unique TIANRed reagent and the real-time monitoring of lysis status ensure the

high efficiency of purification.■ Purified plasmid can be used directly in downstream applications.

TIANRed Colour Indicator

Ordering InformationCat. no. QuantityDP114-01 4 platesDP114-02 24 plates



Kit Components 4 plates 24 platesBuffer BL 240 ml 6×250 mlRnase a (10 mg/ml) 1.25 ml 7.2 mlBuffer P1 125 ml 3×240 mlBuffer P2 125 ml 3×240 mlBuffer P3 160 ml 4×250 mlBuffer PD 240 ml 6×250 mlBuffer Pw 3×50 ml 4×250 mlBuffer tB 60 ml 240 mltianRed 700 µl 3.6 ml96 Plate cP3 4 Plates 24 Plates96 Filtration Plate 4 Plates 24 Plates96 collection Plate 16 Plates 96 PlatesPlate Cover 16 Sheets 96 SheetsPermeation Sheet 5 Sheets 25 Sheets


5~6 μg high-quality pBS plasmid could be extracted from 1 ml E.coli suspension (LB culture) using tianprep Highpure n96 Plasmid Kit.

Accurate Sequencing of Purified PCR Product on ABI PRiSm 3730XL.

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TIANprep Rapid N96 Plasmid KitFor rapid high-throughput purification of small amount of plasmid Dna

Description TIANprep Rapid N96 Plasmid Kit is designed for rapid isolation of high-quality plasmid DNA using uniquely developed 96-well plate and buffer III. The kit deliver DNA yields of 10-20 µg from up to 1.3 ml bacterial culture each well using isopropanol precipitation. This kit is suitable for high-through plasmid DNA purification and the purified plasmid DNA can be used in restriction endonuclease digestion, sequencing, screening, library construction and transformation.

Features■ Extraction of 96 plasmid DNA samples can be finished in 2-3 hours.■ Unique TIANRed reagent and real-time monitoring of lysis status ensure the

high efficiency of purification.■ Purified plasmid DNA can be used directly in downstream applications.

Experimental Procedure:Sample Pretreatment

Ordering InformationCat. no. QuantityDP115-01 4 platesDP115-02 24 plates



Kit Components 4 plates 24 platesRnase a (10 mg/ml) 1.25 ml 7.2 mlBuffer P1 125 ml 3×240 mlBuffer P2 125 ml 3×240 mlBuffer iii 125 ml 3×240 mlBuffer tB 60 ml 240 mltianRed 700 µl 3.6 ml96 Filtration Plate 4 Plates 24 Plates96 collection Plate 8 Plates 48 PlatesPlate Cover 32 Sheets 150 SheetsPermeation Sheet 6 Sheets 25 Sheets


Accurate Sequencing of Purified PCR Product on ABI PRiSm 3730XL

Add Buffer P1, P2, III

Filtration

isopropanol Precipitation

Wash with 70% Ethanol

Air dry to remove ethanol

DNA dissolve

8~10 μg high-quality pBS plasmid was extracted from 1 ml E.coli suspension (LB culture) using tianprep Rapid N96 Plasmid Kit. Gel concentration: 1%; Sample volume: 2 μl; Vector: pBS; Strains: TOP10



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TIANprep Yeast Plasmid DNA KitFor fast and convenient purification of plasmid DNA from yeast

Description the tianprep Yeast Plasmid Dna Kit is designed for rapid isolation of plasmid DNA from yeast cultures. The kit uses silica membrane technology to provide simple and highly efficient method for yeast plasmid DNA preparation. This kit is also suited for E. coli, and can handle multiple samples without the use of toxic reagents. Purified plasmid DNA can be used in restriction endonuclease digestion, sequencing, screening, library construction and transformation.




Kit Components 50 prepsRnase a (10 mg/ml) 150 µlBuffer BL 30 mlBuffer YP1 15 mlBuffer YP2 15 mlBuffer YP3 20 mlBuffer PD 30 mlBuffer Pw 15 mlBuffer eB 15 mlSpin columns cP2 50Collection Tubes (2 ml) 50


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Cat. no. Product Name QuantityDP103-02 tianprep mini Plasmid Kit 50 preps

DP103-03 tianprep mini Plasmid Kit 200 preps

DP104-02 tianpure mini Plasmid Kit 50 preps

DP106-02 tianprep midi Plasmid Kit 50 preps

DP107-02 tianpure midi Plasmid Kit 50 preps

DP112-02 tianprep Yeast Plasmid Dna Kit 50 preps

DP114-01 tianprep Highpure n96 Plasmid Kits 4x96 wells deepwell plates

DP114-02 tianprep Highpure n96 Plasmid Kits 24x96 wells deepwell plates

DP115-01 tianprep Rapid n96 Plasmid Kits 4×96 preps

DP115-02 tianprep Rapid n96 Plasmid Kits 24×96 preps

DP116 HighPure maxi Plasmid Kit 10 preps

DP117 endoFree maxi Plasmid Kit 10 preps

Cat. no. Product Name QuantityRK101-01 Buffer P1 15 ml

RK101-02 Buffer P1 60 ml





RK105-01 Buffer PD 30 ml

RK105-02 Buffer PD 120 ml

RK109 Buffer P4 80 ml

RK113-01 Buffer Pw 15 ml

RK113-02 Buffer Pw 50 ml

RK120-02 Buffer eB 30 ml

RK126-c Spin column cP2 1





RK135 Filtration cS 1

RK136 Filtration cS1 1

RK140 Buffer YP1 15 ml



Rt410-02 Lyticase (10 U/µl) 3000 U

Plasmid DNA Purification Individual Buffers and Accessories


PCR Cleanup & Gel Extraction


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100 bp – 20 kb fragments

DP212 TIANquick Oligo Purification Kit

DP205 TIANquick Maxi Purification Kit

DP213 TIANquick N96 Purification Kit

DP204 TIANquick Midi Purification Kit

20 bp – 10 kb fragments

DP214 Universal DNA Purification Kit

DP209 TIANgel Midi Purification Kit

DP210 TIANgel Maxi Purification Kit

Pc

R c

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PcR cleanup

Universal(PcR cleanup & gel extraction)

gel extraction

PCR Cleanup & Gel Extraction Products

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TIANquick Midi Purification KitFor purification of PcR products (100 bp -10 kb) with > 80% recovery efficiency

Description TIANquick Midi Purification Kit provides unique spin columns, buffers, and collection tubes for silica-membrane-based purification of PcR products. the recovery yield is more than 80% for 100 bp -10 kb Dna fragments (30%-50% yields for <100 bp or >10 kb Dna fragments). this kit effectively removes proteins, salts, and small fragment impurities. It can also be used for purification of DNA fragments from enzymatic reaction solutions. Purified DNA can be directly used in applications such as restriction enzyme digestion, PCR amplification, sequencing, hybridization, and transformation, etc.

Features■ Fast and simple DNA recovery that can be finished in 15 min.■ Specific spin columns and unique buffers ensure >80% recovery of high-purity

Dna.■ 10 µg Dna binding capacity for each spin column.


Contents and StorageKit Components 50 preps 200 prepsBuffer BL 30 ml 120 mlBuffer PB 30 ml 120 mlBuffer Pw 15 ml 50 mlBuffer eB 15 ml 30 mlSpin columns cB2 50 200collection tubes (2 ml) 50 200



100 bp Dna fragments were purif ied using TIANquick Midi Purification Kit1: Before purification 2: After purificationm: tiangen 50 bp Dna marker

8 kb DNA fragments were purified using TIANquick Midi Purification Kit1: Before purification 2: After purificationm: tiangen 1 kb Dna marker

m 1 2

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TIANquick Maxi Purification KitFor purification of large amount of PCR products (100 bp -20 kb)

Description TIANquick Maxi Purification Kit provides unique spin columns, buffers and collection tubes for silica membrane based purification of PcR products. the recovery yield is more than 80% for 100 bp -10 kb Dna fragments (30%-50% yields for <100 bp or >10 kb Dna fragments). this kit effectively removes proteins, salts, small fragments and other impurities. It can also be used for purification of DNA fragments from enzymatic reaction solutions. Purified DNA can be directly used in applications such as restriction enzyme digestion, PCR amplification, sequencing, hybridization, and transformation, etc.


Dna.■ 20 µg Dna binding capacity for each spin column.


Contents and StorageKit Components 50 prepsBuffer BL 30 mlBuffer PB 60 mlBuffer Pw 15 mlBuffer eB 15 mlSpin columns cB3 50collection tubes (2 ml) 50


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TIANquick N96 Purification KitFor high-throughput purification of 100 bp - 10 kb DNA fragments

Description TIANquick N96 Purification Kit provides 96-well plates, buffers and collection tubes for high-throughput silica-membrane-based purification of PCR products. This kit effectively removes proteins, organic compounds, salts, oligonucleotide and other impurities. Purified DNA can be directly used in applications such as restriction enzyme digestion, PCR amplification, sequencing, library construction, and transformation, etc.

Features■ 100 bp - 10 kb DNA fragments are effectively purified.■ 5 µg Dna binding capacity for each well.■ High-purity purified Dna fragments can be directly used in downstream

applications.

Ordering InformationCat. no. QuantityDP213-01 4 PlatesDP213-02 24 Plates

Contents and StorageKit Components 4×96 preps 24×96 prepsBuffer BL 240 ml 6×250 mlBuffer PB 240 ml 6×250 mlBuffer Pw 3×50 ml 4×250 mlBuffer tB 60 ml 240 ml96 Plates cB2 4 2496 collection Plates 8 48Plate cover 4 24


Experimental Example:Experimental Example:

900 bp, 3500 bp, 4500 bp DNA fragments were purified using TIANquick N96 Purification Kit1, 2, 3: DNA purified using TIANquick N96 Purification Kit4: DNA purified using purification kit from another famous company

1 2 3 4 1 2 3 4 1 2 3 4

900 bp 3500 bp 4500 bp

Sample Pretreatment Balance

Buffer PB

Dna Binding

wash

Remove ethanol

Dna elution



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TIANquick Oligo Purification KitFor efficient purification of oligonucleotide fragments

Description TIANquick Oligo Purification Kit provides a simple, fast and high-effective oligonucleotide purification from solutions (PCR products, enzymatic reactions, etc). Fragments ranging from 20 bp to 10 kb can be effectively purified by using specially developed spin column ca2. Purified Dna can be directly used in applications such as restriction enzyme digestion, PCR amplification, sequencing, hybridization and transformation, etc.

Features■ Especially suited to the purification of probes.


Contents and StorageKit Components 4×96 prepsBuffer BL 30 mlBuffer PS 30 mlBuffer Pw 15 mlBuffer eB 15 mlSpin columns ca2 20collection tubes (2 ml) 20


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Universal DNA Purification KitFor universal purification of Dna fragments from both gels and solutions

Description Universal DNA Purification Kit provides a fast, simple and high-effective DNA purification from agarose gel or solution (PCR products, enzymatic reaction, etc). this kit is effective for any type of agarose in either tae or tBe buffer. Buffer PC contains a pH indicator, allowing determination of the optimal pH for DNA binding. DNA fragments ranging from 100 bp to 8 kb can be effectively purified by using a specially developed spin column CB2. The purification efficiency is more than 80%. Dna fragments purified with the Universal Dna Purification Kit can be used directly in downstream applications such as restriction enzyme digestion, PCR amplification, sequencing, hybridization and transformation, etc.

Features■ excellent compatibility that fulfils the needs of purification of Dna fragments

from both gels and solutions.■ Stable process plus pH indicator allow the easy determination of the optimal pH

for Dna binding.

pH Indicator Dye


Contents and StorageKit Components 50 preps 200 prepsBuffer Pc 25 ml 100 mlBuffer BL 30 ml 120 mlBuffer Pw 15 ml 50 mlBuffer eB 15 ml 30 mlSpin columns cB2 50 200collection tubes (2 ml) 50 200


Experimental Example:

m 100% 80% 60% 40% 20% Supplier a tiangen

100% 80% 60% 40% 20% Supplier a tiangen

Before extraction

Before extraction

pH indicator Dye: easy visual determination of optimal pH for Dna adsorption.

DNA adsorption requires pH ≤7.5, and the pH indicator in the Buffer Pc will appear yellow in this range. If the solution is orange or violet, add 10 μl 3M CH3coona (pH 5.0) to the solution until the solution becomes yellow.

100 bp (A) or 10 kb (B) Dna fragments before extraction in different dilution percentage and after extraction with TIANGEN Universal DNA Purification Kit (TIANGEN), or a silica-based DNA purification kit from Supplier a (Supplier A). Recoveries of approximately 80% are obtained for both fragment sizes by Universal DNA Purification Kit.

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TIANgel Midi Purification KitFor gel extraction of Dna fragments (100 bp -10 kb) with > 80% recovery efficiency

Description TIANgel Midi Purification Kit is designed to purify DNA fragments from agarose gels. the simple procedure uses silica-based spin columns to purify and concentrate Dna fragments from 100 bp to 20 kb in about 15 minutes. the recovery efficiency is more than 80% for 100 bp -10 kb DNA fragments (30%-50% yields for <100 bp or >10 kb Dna fragments). the purified Dna can be used for restriction enzyme digestion, PCR analysis, sequencing, DNA ligation, and transformation procedures.


Dna fragments.■ 10 µg Dna binding capacity for each spin column.


Contents and StorageKit Components 50 preps 200 prepsBuffer BL 30 ml 120 mlBuffer Pn 50 ml 2 × 100 mlBuffer Pw 15 ml 50 mlBuffer eB 15 ml 30 mlSpin columns ca2 50 200collection tubes (2 ml) 50 200


Experimental Example:

100 bp Dna fragments were purified using tiangel midi Purification Kit1: Before Purification 2: After Purificationm: tiangen 50 bp Dna Ladder

10 kb Dna fragments were purified using tiangel midi Purification Kit1: Before Purification 2: After Purificationm: tiangen Dna marker D15000

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TIANgel Maxi Purification KitFor large scale Dna clean up from agarose gel using silica-membrane columns

Description TIANgel Maxi Purification Kit provides unique buffer system to purify DNA fragments from any type of agarose in either tae or tBe buffer. the simple procedure uses silica-based spin columns to purify and concentrate Dna fragments from 100 bp to 20 kb in about 15 minutes. the recovery efficiency is more than 80% for 100 bp -10 kb Dna fragments (30%-50% yields for <100 bp or >10 kb Dna fragments). the purified Dna can be used for restriction enzyme digestion, PCR analysis, sequencing, DNA ligation, and transformation procedures.

Features■ Fast and simple DNA recovery that can be finished in 15 min.■ Specific spin columns and unique buffers to ensure >80% recovery of high-

purity Dna.■ 20 µg Dna binding capacity for each spin column.




Kit Components 50 prepsBuffer BL 30 mlBuffer Pn 100 mlBuffer Pw 15 mlBuffer eB 15 mlSpin columns ca3 50collection tubes (2 ml) 50




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Cat. no. Product Name QuantityRK106-01 Buffer PB 30 ml

RK106-03 Buffer PB 120 ml

RK107 Buffer PS 30 ml

RK108-01 Buffer PN 50 ml

RK108-02 Buffer PN 100 ml

RK110-01 Buffer PC 25 ml

RK110-02 Buffer PC 100 ml

RK113-01 Buffer PW 15 ml

RK120-02 Buffer EB 30 ml

RK125-A Spin Column CA1 50

RK125-B Spin Column CB1 50





RK150-01 Buffer BL 30 ml

RK150-03 Buffer BL 120 ml

PCR Cleanup & Gel Extraction Individual Buffers and Accessories


Cat. no. Product Name QuantityDP204-02 TIANquick Midi Purification Kit 50 preps

DP204-03 TIANquick Midi Purification Kit 200 preps

DP205-02 TIANquick Maxi Purification Kit 50 preps

DP209-02 TIANgel Midi Purification Kit 50 preps

DP209-03 TIANgel Midi Purification Kit 200 preps

DP210-02 TIANgel Maxi Purification Kit 50 preps

DP212-01 TIANquick Oligo Purification Kit 20 preps

DP213-01 TIANquick N96 Purification Kit 4×96 preps

DP213-02 TIANquick N96 Purification Kit 24×96 preps

DP214-02 Universal DNA Purification Kit 50 preps

DP214-03 Universal DNA Purification Kit 200 preps

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精准、清晰的DNA分子量Marker精准、清晰的DNA分子量Marker

DNA Molecular Weight Marker


Cat. no. Product Name QuantitymD101-01 Dna marker i 50 preps

mD101-02 Dna marker i 200 preps

mD102-01 Dna marker ii 50 preps

mD102-02 Dna marker ii 200 preps

mD103-01 Dna marker iii 50 preps

mD103-02 Dna marker iii 200 preps

mD104-01 Dna marker iV 50 preps

mD104-02 Dna marker iV 200 preps

mD105-01 Dna marker V 50 preps

mD105-02 Dna marker V 200 preps

mD107-01 Dna marker Vii 50 preps

mD107-02 Dna marker Vii 200 preps

mD108-01 50 bp Dna Ladder 50 preps








mD111-01 1 kb Dna Ladder 50 preps

mD111-02 1 kb Dna Ladder 200 preps

mD113-01 1 kb Plus Dna Ladder 50 preps

mD113-02 1 kb Plus Dna Ladder 200 preps

mD114-01 D2000 50 preps

mD114-02 D2000 200 preps

mD110-01 D15000 50 preps

mD110-02 D15000 200 preps

mD116-01 D15000+2000 50 preps

mD116-02 D15000+2000 200 preps

MD202-01 λ DAN/Hind III 50 preps

MD202-02 λ DAN/Hind III 200 preps

MD206-01 pBR322 DNA/Msp I 20 preps

MD206-02 pBR322 DNA/Msp I 100 preps

MD203-01 pUC18 DNA/Msp I 20 preps

MD203-02 pUC18 DNA/Msp I 100 preps


DNA Molecular Weight Marker



Cat. no. Product Name SizeKT201-01 2×Taq PCR MasterMix (with loading dye) 1 ml

KT201-02 2×Taq PCR MasterMix (with loading dye) 5×1 ml

KT201-11 2×Taq PCR MasterMix 1 ml

KT201-12 2×Taq PCR MasterMix 5×1 ml

KP201-01 2×Pfu PCR MasterMix (with loading dye) 0.5 ml

KP201-02 2×Pfu PCR MasterMix (with loading dye) 5×1 ml

KP201-11 2×Pfu PCR MasterMix 0.5 ml

KP201-12 2×Pfu PCR MasterMix 5×1 ml

KT203-01 2×Long Taq PCR MasterMix (with loading dye) 0.5 ml

KT203-02 2×Long Taq PCR MasterMix (with loading dye) 5×1 ml

KT203-11 2×Long Taq PCR MasterMix 0.5 ml

KT203-12 2×Long Taq PCR MasterMix 5×1 ml

KT204-01 2×Taq Platinum PCR MasterMix (with loading dye) 0.5 ml

KT204-02 2×Taq Platinum PCR MasterMix (with loading dye) 5×1 ml

KT204-11 2×Taq Platinum PCR MasterMix 0.5 ml

KT204-12 2×Taq Platinum PCR MasterMix 5×1 ml

KT205-01 2×Taq Plus PCR MasterMix (with loading dye) 0.5 ml

KT205-02 2×Taq Plus PCR MasterMix (with loading dye) 5×1 ml

KT205-11 2×Taq Plus PCR MasterMix 0.5 ml

KT205-12 2×Taq Plus PCR MasterMix 5×1 ml

KT206-01 2×GC-rich PCR MasterMix (with loading dye) 0.5 ml

KT206-11 2×GC-rich PCR MasterMix 0.5 ml

KT208-01 2×HotMaster PCR MasterMix (with loading dye) 0.5 ml

KT208-02 2×HotMaster PCR MasterMix (with loading dye) 5×1 ml

KT208-11 2×HotMaster PCR MasterMix 0.5 ml

KT208-12 2×HotMaster PCR MasterMix 5×1 ml

KT501-11 Golden Fast PCR Kit 1 ml

KT501-12 Golden Fast PCR Kit 5 ml

TIANGEN 2×PCR Master Mix

Documents

Overview of Genomic DNA Purification 1 · 2013-04-11 · Overview of Genomic DNA Purification e-mail:[email protected] 1 DP318 tianamp Blood Dna Kit (0.1-1 ml) Genomic DNA Purification