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PEG precipitation of the PCR product Used Eppendorf 5413 Centrifuge equipped with a drum rotor. Custom strip cap tube holders hold 4x8 = 32 tubes each. It is critical to precipitate the DNA to the bottom of the tube. For difficult ORFs use double precipitation to decrease background. - PowerPoint PPT Presentation
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PEG precipitation of the PCR productUsed Eppendorf 5413 Centrifuge equipped with a drum rotor.Custom strip cap tube holders hold 4x8 = 32 tubes each.It is critical to precipitate the DNA to the bottom of the tube.For difficult ORFs use double precipitation to decrease background.