PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

War Time:War Time: A Universal Microarray BiochiA Universal Microarray Biochip for detection of p for detection of SARS and anthraxSARS and anthrax

Chih-Wei Lin (D937708)Chih-Wei Lin (D937708)

Nov.3, 2006Nov.3, 2006

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

War Time…War Time…

In In May, 20, 2006May, 20, 2006, Our , Our country is attacked by the country is attacked by the biochemical weapon. biochemical weapon.

““We need compact We need compact diagnostics systems to diagnostics systems to provide triage to screen provide triage to screen large numbers of people large numbers of people against the outbreaks of against the outbreaks of diseases that are bogging diseases that are bogging down our local healthcare down our local healthcare systems”systems” Our brave Our brave President Liu said.President Liu said.

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

Bioweapon contain…Bioweapon contain…

SARSSARS AnthraxAnthrax

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

SARSSARS

Severe Acute Respiratory Syndrome (SARS)Severe Acute Respiratory Syndrome (SARS) is cau is caused by coronavirus. (SARS-CoV). sed by coronavirus. (SARS-CoV).

In 2003, SARS was first detected in In 2003, SARS was first detected in AsiaAsia. Few mon. Few months letter, SARS spread to ths letter, SARS spread to North America, South ANorth America, South America and Europemerica and Europe. 8,098 people worldwide get S. 8,098 people worldwide get SARS-CoV, and 774 were died. ARS-CoV, and 774 were died.

Generally, when you get SARS, your body temperGenerally, when you get SARS, your body temperature will be ature will be higher than 38.0°Chigher than 38.0°C. Other symptoms . Other symptoms include include headache, discomfort, mild respiratory, dheadache, discomfort, mild respiratory, diarrhea, dry cough, pneumonia and body aches.iarrhea, dry cough, pneumonia and body aches.

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

AnthraxAnthrax

Anthrax is caused by the Anthrax is caused by the spore-forming baspore-forming bacteriumcterium, called , called Bacillus anthracisBacillus anthracis. .

A a disease of A a disease of animalanimal (cattle, sheep, camel (cattle, sheep, camels, and other herbivores) and s, and other herbivores) and humanshumans..

Detected in Detected in South and Central America, SoSouth and Central America, Southern and Eastern Europe, Asia, Africa, thuthern and Eastern Europe, Asia, Africa, the Caribbean, and the Middle Easte Caribbean, and the Middle East. .

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

Symptoms of Symptoms of AnthraxAnthrax

SkinSkin: skin infection will raise an itchy bump, : skin infection will raise an itchy bump, usually 1-3 cm in diameter, and with a dying usually 1-3 cm in diameter, and with a dying area in the bump center. Then, lymph in the area in the bump center. Then, lymph in the nearby area will swell. nearby area will swell. About 20% will result in About 20% will result in deathdeath..

AirAir: In the beginning, symptoms may like a : In the beginning, symptoms may like a common cold. Several days later, the symptoms common cold. Several days later, the symptoms will be severe breathing problems. will be severe breathing problems.

IntestinalIntestinal: intestinal infection will be nausea, loss : intestinal infection will be nausea, loss of appetite, vomiting, fever are followed by of appetite, vomiting, fever are followed by abdominal pain, vomiting blood, and severe abdominal pain, vomiting blood, and severe diarrhea, and which will diarrhea, and which will result in death in 25% to result in death in 25% to 60%.60%.

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

About PMECAbout PMEC

Power MEMS Company (PMC)Power MEMS Company (PMC) is a world-le is a world-leading MEMS group, located in Hsinchu, anading MEMS group, located in Hsinchu, and cooperates with many IC companies, sud cooperates with many IC companies, such as TSMC and UMC. ch as TSMC and UMC.

We have set up the We have set up the world-class laboratoryworld-class laboratory and employ the and employ the best engineerbest engineer. Now, we ca. Now, we can meet customer's demands in the shorten meet customer's demands in the shortest time. st time.

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

War time…War time…

Now, our country is facing the Now, our country is facing the great danger and great danger and disasterdisaster. Anyone should do their best to help our . Anyone should do their best to help our country to spend this difficulty. For this reason, country to spend this difficulty. For this reason, within the shortest time, we combine two key tecwithin the shortest time, we combine two key technologies, hnologies, real time polymerase chain reactionreal time polymerase chain reaction a and nd bio-bar code DNA detection techniquebio-bar code DNA detection technique, to des, to design such a power chip to check whether the patieign such a power chip to check whether the patient is infected the SARS or anthrax. Its name is nt is infected the SARS or anthrax. Its name is ““Universal Microarray Biochip-L1”Universal Microarray Biochip-L1”

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

Bio-bar code DNA Bio-bar code DNA detection technique detection technique Bio-bar code DNA detection technique is invented in 2004. It is aBio-bar code DNA detection technique is invented in 2004. It is a

ble to detect 10 strands of target DNA in a 30 microlitre sample.ble to detect 10 strands of target DNA in a 30 microlitre sample.

Bio-bar code DNA detection technique

Gold particle

Bar-code DNA (specimen)

Identify DNA

Ferric oxide

Bar-code DNA (specimen)

Magnetic Field

1.

Heating2.

High concentration

3.Plot by Chih-Wei Lin

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

Real Time Polymerase Real Time Polymerase Chain Reaction (RT Chain Reaction (RT PCR)PCR) Real Time Polymerase Chain ReaReal Time Polymerase Chain Rea

ction (RT PCR)ction (RT PCR) is a technique to is a technique to monitor the progress of a PCR remonitor the progress of a PCR reaction in real time.action in real time.

By this method, a relatively small By this method, a relatively small amount of DNA or RNA can be qamount of DNA or RNA can be quantified. uantified.

It was found that the six nucleotiIt was found that the six nucleotide positions de positions (nt9404, nt9479, nt1(nt9404, nt9479, nt19838, nt21721, nt22222, and nt279838, nt21721, nt22222, and nt27827)827) have an important relationsh have an important relationship to detect ip to detect 40 SARS-CoV40 SARS-CoV geno genome sequences and two SARS-like me sequences and two SARS-like coronavirus genome sequences.coronavirus genome sequences.

92~95℃

40~52℃

1.

2.

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

Universal Microarray BiocUniversal Microarray Biochip-L1hip-L1

Micro glass slides

Micro array strucure

RT PCR and Bio-bar code DNA detection processes

Results analysis

Universal Microarray Biochip-L1 Detection process

Plot by PMC Chih-Wei Lin

PME Company, Chih-Wei LinPME Company, Chih-Wei Lin

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