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RNA DISCOVERY SOLUTIONS
Providing Sample to Insight
QIAGEN RNA DISCOVERY SOLUTIONS – PROVIDING SAMPLE TO INSIGHT
Reliable results in end-point RT-PCR, real-time RT-PCR, microarray and next-generation sequencing analysis
depend strongly on the quality of the RNA sample used. To ensure success in your experiments, QIAGEN
provides a comprehensive range of RNA sample technologies that deliver high-quality RNA from biological
samples. Innovative advancements in the QuantiNova product range offering cutting-edge solutions for
real-time PCR, minimizing experimental errors and artefacts. Array and NGS-based technologies allow
researchers to gain more insight into individual samples using either a disease or pathway specific approach.
Let us aid you in finding the best approach to generate relevant, actionable findings.
QIAGEN RNA WORKFLOW SOLUTIONS
TARGETED ARRAYS &
SOLUTIONS FOR RNA
FOR SINGLE CELL
PCR ASSAY KITS
01 | SAMPLE COLLECTION AND STABILIZATION
Once a biological sample is harvested, its RNA
becomes extremely unstable. Immediate stabilization
of cellular RNA is critical to minimize pre-analytical
variables and to ensure accurate and reproducible
• Allprotect Tissue Reagent
• RNAlater Reagent
• RNAlater TissueProtect Tubes
• RNeasy Protect Mini and Midi Kits
• RNAprotect Cell Reagent
• RNeasy Protect Cell Mini Kit
• RNAprotect Bacteria Reagent
• RNeasy Protect Bacteria Mini Kit
• RNeasy Protect Saliva Mini Kit
• RNAprotect Animal Blood Tubes
• RNeasy Protect Animal Blood Kit
• miRNeasy Protect Animal Blood Kit
• PAXgene Blood RNA Tubes
• PAXgene Blood RNA Kit
• PAXgene Blood miRNA Kit
• PAXgene Bone Marrow RNA Tubes
• PAXgene Bone Marrow RNA Kit
• PAXgene Tissue Containers
• PAXgene Tissue RNA Kit
• PAXgene Tissue miRNA Kit
02 | RNA EXTRACTION AND PURIFICATION
When purifying RNA it is essential to utilize a method that maintains RNA integrity and effectively removes
gDNA, contaminants and inhibitors, all of which can affect downstream applications.
• QIAamp RNA
• RNeasy Plus
• RNeasy Plus
• RNeasy Lipid Tissue
• RNeasy Fibrous
• RNeasy Plus
• RNeasy Micro
• RNeasy FFPE
• RNeasy Plant
• QIAamp Viral RNA Mini
• QIAamp DSP Virus
• QIAamp MinElute Virus Spin
• QIAamp MinElute Virus Vacuum
• QIAamp UltraSens Virus
• RNeasy Plus
• miRNeasy • RNeasy Plus
• miRNeasy • exoRNeasy • RNeasy Plus
• AllPrep DNA/RNA Micro
• Allprep DNA/RNA Mini
• AllPrep RNA/Protein
• AllPrep DNA/
• RNeasy Plus
• miRNeasy FFPE • RNeasy FFPE
03 | PRE-AMPLIFICATION FOR SINGLE CELL ANALYSIS/LIMITED SAMPLE AMOUNTS
Pre-amplification overcomes the limitations imposed by limited RNA availability, enabling downstream
investigation of the transcriptome with minimal bias.
REPLI-g WTA Single Cell Kit
For whole transcriptome amplification of total RNA or mRNA from single cells (1-1000 cells).
REPLI-g Single Cell RNA Library Kit (Illumina)
Contains novel REPLI-g SensiPhi DNA Polymerase, as well as an optimized set of buffers and reagents for
whole transcriptome amplification (WTA) from just single cells (up to 1000 cells) or equivalently small
REPLI-g Cell WGA & WTA Kit
For parallel whole genome and whole transcriptome amplification from cells and limited samples
RT² PreAMP cDNA Synthesis Kit
For pre-amplification of cDNA from small or FFPE samples of RNA for analysis on RT² Profiler PCR Arrays.
miScript PreAMP PCR Kit
For pre-amplification of cDNA from samples containing low RNA amounts prior to real-time PCR analysis
of miRNA using miScript PCR Arrays.
04 | END-POINT RT-PCR SOLUTIONS
QIAGEN’s new OneStep Ahead RT-PCR Kit offers faster one-step RT-PCR (1-hour protocol) with high sensitivity,
specificity and fidelity. The unique two-phase hot-start procedure allows for room-temperature setup and
optional pipetting control offer increased convenience. The dedicated enzyme mix improves specificity,
sensitivity (0.1pg to 2µg) and fidelity.
05 | QUANTINOVA REAL-TIME PCR ASSAY KITS
QIAGEN’s new range of QuantiNova kits offer a breakthrough in real-time PCR technologies. In-process safety
features assist in eliminating variables and minimizing artefacts. QuantiNova kits offer the added convenience
of room temperature setup and in-built visual pipetting controls. QuantiNova kits are compatible will all
real-time PCR instruments, irrespective of format, and provide consistent, sensitive and reproducible results.
QuantiNova Reverse Transcription Kit
Allows for fast cDNA synthesis and efficient removal of contaminating gDNA through the use of an
integrated gDNA removal mix. Includes internal control RNA to assess for successful reverse
transcription and amplification (requires separate QuantiNova IC assay and appropriate assay kit for
QuantiNova SYBR Green PCR Kit
Accurate and robust detection down to single target copy targets using ultrafast cycling protocol.
Superior sensitivity and reaction efficiencies.
QuantiNova Probe PCR Kit
Highly sensitive, specific, and ultrafast, probe-based real-time PCR. Duplex capability. Robust detection
down to single copy targets. Stable for up to 100 hours at 30�C.
QuantiNova SYBR Green RT-PCR Kit
Unique two-phase hot-start procedure for room temperature setup. Internal control for positive
in-process verification of successful RT-PCR. Ultrafast, universal protocol for all standard and fast cyclers.
QuantiNova Probe RT-PCR Kit
One-step qRT-PCR using sequence-specific probes for gene expression analysis. Unique two-phase
hot-start procedure for room temperature setup. gDNA reduction for improved quantification accuracy.
QuantiNova IC Probe Assay (primer/probe mix)
Detects QuantiNova Internal Control RNA; use with QuantiNova Probe PCR Kit or QuantiNova Probe
QuantiNova IC SYBR Green Assay (primer mix)
For SYBR-based detection of QuantiNova Internal Control RNA; use with QuantiNova SYBR Green PCR Kit
or QuantiNova SYBR Green RT-PCR Kit.
QuantiTect Primer Assays
Genome-wide, bioinformatically validated primer sets for use in SYBR Green-based real-time RT-PCR on
any cycler. Assays are available for all genes from human, rat, mouse, and many other species.
Isolate RNA from research samples:
cells, tissues, FFPE, and/or blood
Add cDNA to RT²
SYBR Green qPCR Mastermix;
aliquot accross RT²Profiler PCR
RNA to cDNA with
RT² First Strand Kit
Generate data in any
real-time PCR instrument
Analyse your data
Interpret your data and publish
06 | TARGETED ARRAYS & PANELS
Arrays allow researchers to evaluate more data points in a single experiment, reducing sample input
• RT² Profiler PCR Arrays
• miScript PCR System
• QIAseq Targeted RNA and Targeted Virtual Panels
06.01 | RT² PROFILER PCR ARRAYS
Data analysis is simplified with an easy to use Microsoft Excel analysis macro. Alternatively, researchers can
utilize the GeneGlobe Data Analysis Center:
• Intuitive, user-friendly interface with no bioinformatics skills needed
• Complimentary data analysis for all GeneGlobe assays and arrays
• Get results i