QIAGEN RNA Workflow Solutions - At GeneGlobe, you can discover and order gene- and pathway-speciï¬پc

  • View
    0

  • Download
    0

Embed Size (px)

Text of QIAGEN RNA Workflow Solutions - At GeneGlobe, you can discover and order gene- and...

  • WhiteSci Whitehead Scientific (Pty) Ltd

    Products. Expertise. Support.

    RNA DISCOVERY SOLUTIONS Providing Sample to Insight

  • QIAGEN RNA DISCOVERY SOLUTIONS – PROVIDING SAMPLE TO INSIGHT

    Reliable results in end-point RT-PCR, real-time RT-PCR, microarray and next-generation sequencing analysis depend strongly on the quality of the RNA sample used. To ensure success in your experiments, QIAGEN provides a comprehensive range of RNA sample technologies that deliver high-quality RNA from biological samples. Innovative advancements in the QuantiNova product range offering cutting-edge solutions for real-time PCR, minimizing experimental errors and artefacts. Array and NGS-based technologies allow researchers to gain more insight into individual samples using either a disease or pathway specific approach. Let us aid you in finding the best approach to generate relevant, actionable findings.

    QIAGEN RNA WORKFLOW SOLUTIONS

    RNA EXTRACTION AND PURIFICATION

    END-POINT RT-PCR SOLUTIONS

    TARGETED ARRAYS & PANELS

    AUTOMATION SOLUTIONS FOR RNA ANALYSIS

    SAMPLE COLLECTION AND STABILIZATION

    1

    2

    3

    4

    5

    6

    7

    8

    PRE-AMPLIFICATION FOR SINGLE CELL

    ANALYSIS/LIMITED SAMPLE AMOUNTS

    QUANTINOVA REAL-TIME PCR ASSAY KITS

    GENEGLOBE PORTAL

  • 01 | SAMPLE COLLECTION AND STABILIZATION

    Once a biological sample is harvested, its RNA becomes extremely unstable. Immediate stabilization of cellular RNA is critical to minimize pre-analytical variables and to ensure accurate and reproducible analysis.

    Sample Type

    Kit

    Human Blood

    Tissue

    Cells

    Bacteria

    Saliva

    Animal Blood

    Human Blood

    Human Bone Marrow

    Fixed Tissue

    • Allprotect Tissue Reagent • RNAlater Reagent • RNAlater TissueProtect Tubes • RNeasy Protect Mini and Midi Kits

    • RNAprotect Cell Reagent • RNeasy Protect Cell Mini Kit

    • RNAprotect Bacteria Reagent • RNeasy Protect Bacteria Mini Kit

    • RNeasy Protect Saliva Mini Kit

    • RNAprotect Animal Blood Tubes • RNeasy Protect Animal Blood Kit • miRNeasy Protect Animal Blood Kit

    • PAXgene Blood RNA Tubes • PAXgene Blood RNA Kit • PAXgene Blood miRNA Kit

    • PAXgene Bone Marrow RNA Tubes • PAXgene Bone Marrow RNA Kit

    • PAXgene Tissue Containers • PAXgene Tissue RNA Kit • PAXgene Tissue miRNA Kit

  • 02 | RNA EXTRACTION AND PURIFICATION

    When purifying RNA it is essential to utilize a method that maintains RNA integrity and effectively removes gDNA, contaminants and inhibitors, all of which can affect downstream applications.

    RNA incl. miRNA

    Analyte RNA

    Human Blood

    Human Blood

    Serum/ plasma (cell-free body fluids*)

    Tissue Easy to lyse

    Tissue Difficult to lyse

    Cells or cell super- natants

    Limited sample amount

    FFPE Tissue Sections

    Plant

    Animal Blood

    Circulating nucleic acid

    RNA clean-up

    Exosomal RNA

    RNA incl. gDNA

    removal

    DNA, RNA and Protein

    Viral RNA

    • QIAamp RNA Blood

    • PAXgene Blood System

    • RNeasy Plus

    • RNeasy Plus Universal • RNeasy Lipid Tissue • RNeasy Fibrous Tissue

    • RNeasy Plus

    • RNeasy Micro

    • RNeasy FFPE

    • RNeasy Plant

    • RNeasy MinElute

    • PAXgene Blood System

    • RNeasy MinElute

    • RNeasy MinElute

    • miRNeasy Serum/Plasma

    • exoRNeasy

    • QIAamp Viral RNA Mini • QIAamp DSP Virus • QIAamp MinElute Virus Spin • QIAamp MinElute Virus Vacuum • QIAamp UltraSens Virus

    • QIAamp Circulating Nucleic Acid

    • RNeasy Plus Universal

    • miRNeasy • RNeasy Plus

    • miRNeasy

    • miRNeasy • exoRNeasy • RNeasy Plus

    • AllPrep DNA/RNA Micro • Allprep DNA/RNA Mini • AllPrep RNA/Protein • AllPrep DNA/ RNA/Protein Mini

    • miRNeasy Micro

    • RNeasy Plus Micro

    • miRNeasy FFPE • RNeasy FFPE

    • miRNeasy

    • RNeasy Protect Animal Blood

    • RNeasy Protect Animal Blood

    • RNeasy Protect Animal Blood

    • RNeasy Protect Animal Blood

    • RNeasy MinElute

    • RNeasy MinElute

    • RNeasy MinElute

    • RNeasy MinElute

    • RNeasy MinElute

    • RNeasy MinElute

  • 03 | PRE-AMPLIFICATION FOR SINGLE CELL ANALYSIS/LIMITED SAMPLE AMOUNTS

    Pre-amplification overcomes the limitations imposed by limited RNA availability, enabling downstream investigation of the transcriptome with minimal bias.

    REPLI-g WTA Single Cell Kit For whole transcriptome amplification of total RNA or mRNA from single cells (1-1000 cells).

    REPLI-g Single Cell RNA Library Kit (Illumina) Contains novel REPLI-g SensiPhi DNA Polymerase, as well as an optimized set of buffers and reagents for whole transcriptome amplification (WTA) from just single cells (up to 1000 cells) or equivalently small samples.

    REPLI-g Cell WGA & WTA Kit For parallel whole genome and whole transcriptome amplification from cells and limited samples (25-1000 cells).

    RT² PreAMP cDNA Synthesis Kit For pre-amplification of cDNA from small or FFPE samples of RNA for analysis on RT² Profiler PCR Arrays.

    miScript PreAMP PCR Kit For pre-amplification of cDNA from samples containing low RNA amounts prior to real-time PCR analysis of miRNA using miScript PCR Arrays.

  • 04 | END-POINT RT-PCR SOLUTIONS

    QIAGEN’s new OneStep Ahead RT-PCR Kit offers faster one-step RT-PCR (1-hour protocol) with high sensitivity, specificity and fidelity. The unique two-phase hot-start procedure allows for room-temperature setup and optional pipetting control offer increased convenience. The dedicated enzyme mix improves specificity, sensitivity (0.1pg to 2µg) and fidelity.

  • 05 | QUANTINOVA REAL-TIME PCR ASSAY KITS

    QIAGEN’s new range of QuantiNova kits offer a breakthrough in real-time PCR technologies. In-process safety features assist in eliminating variables and minimizing artefacts. QuantiNova kits offer the added convenience of room temperature setup and in-built visual pipetting controls. QuantiNova kits are compatible will all real-time PCR instruments, irrespective of format, and provide consistent, sensitive and reproducible results.

    QuantiNova Reverse Transcription Kit Allows for fast cDNA synthesis and efficient removal of contaminating gDNA through the use of an integrated gDNA removal mix. Includes internal control RNA to assess for successful reverse transcription and amplification (requires separate QuantiNova IC assay and appropriate assay kit for detection).

    QuantiNova SYBR Green PCR Kit Accurate and robust detection down to single target copy targets using ultrafast cycling protocol. Superior sensitivity and reaction efficiencies.

    QuantiNova Probe PCR Kit Highly sensitive, specific, and ultrafast, probe-based real-time PCR. Duplex capability. Robust detection down to single copy targets. Stable for up to 100 hours at 30�C.

    QuantiNova SYBR Green RT-PCR Kit Unique two-phase hot-start procedure for room temperature setup. Internal control for positive in-process verification of successful RT-PCR. Ultrafast, universal protocol for all standard and fast cyclers.

    QuantiNova Probe RT-PCR Kit One-step qRT-PCR using sequence-specific probes for gene expression analysis. Unique two-phase hot-start procedure for room temperature setup. gDNA reduction for improved quantification accuracy.

    QuantiNova IC Probe Assay (primer/probe mix) Detects QuantiNova Internal Control RNA; use with QuantiNova Probe PCR Kit or QuantiNova Probe RT-PCR Kit.

    QuantiNova IC SYBR Green Assay (primer mix) For SYBR-based detection of QuantiNova Internal Control RNA; use with QuantiNova SYBR Green PCR Kit or QuantiNova SYBR Green RT-PCR Kit.

    QuantiTect Primer Assays Genome-wide, bioinformatically validated primer sets for use in SYBR Green-based real-time RT-PCR on any cycler. Assays are available for all genes from human, rat, mouse, and many other species.

  • Isolate RNA from research samples: cells, tissues, FFPE, and/or blood

    Add cDNA to RT² SYBR Green qPCR Mastermix; aliquot accross RT²Profiler PCR

    Array

    Convert total RNA to cDNA with RT² First Strand Kit

    Generate data in any real-time PCR instrument

    Analyse your data

    Interpret your data and publish your results

    Control Experimental

    06 | TARGETED ARRAYS & PANELS

    Arrays allow researchers to evaluate more data points in a single experiment, reducing sample input requirements. • RT² Profiler PCR Arrays • miScript PCR System • QIAseq Targeted RNA and Targeted Virtual Panels

    06.01 | RT² PROFILER PCR ARRAYS

    Data analysis is simplified with an easy to use Microsoft Excel analysis macro. Alternatively, researchers can utilize the GeneGlobe Data Analysis Center: • Intuitive, user-friendly interface with no bioinformatics skills needed • Complimentary data analysis for all GeneGlobe assays and arrays • Get results i

Recommended

View more >