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Report of laboratory

exercises

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Application of flame atomic absorption

spectrometric techniques to pharmaceuticals

and waters

Atomic absorption specroscopy is analytical method characterized with high

selectivity,sensitivity and fast speed.

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Three different brands of multivitamins (effervescent tablets) were taken from the market

and the levels of some elements (total amount) were determined (Ca, Mg, Zn, Cr, Mn)

One tablet from each of the three pharmaceutical products was dissolved in 100ml (mother

solution) water and one milliliter of each solution was diluted 25 times. So were prepared the

working sample solutions and were marked as follows:

Sample 1 (samp1) - ABO Pharma A-Z ;

Sample 2 (samp2) - Supradyn ;

Sample 3 (samp3) - Supravit Active ;

A brand of mineral water - Gorna Banja was tested for any Mg and the concentration of

the metal was under the detection limit of the instrument

For each different element was made a standard calibration curve, using freshly prepared

standard solutions from 0,5; 1; 3; 5; 10; 20ppm containing all the mentioned elements in water

(deionized)

For the last sample results were obtained both after automated calibration and a manually

made calibration curve.

Differences in the absorption using reducing and oxidizing flames were observed

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2.110%3

2.410%2

1.910%1

Measuredvalues

[ug/tablet]

# Sample

Pb calibration curve (ET AAS)

y = 3,775x + 8,3333

R2

= 0,9909

0

50

100

150

200

250

300

350

0 20 40 60 80 100

concentration [ppb]

absorption

=283.3 nm

1.910%3

1.610%2

8.910%1

Measured

values

[ug/tablet]

# Sample

Cd calibration curve (ETAAS)

y = 144,38x + 8,8333

R2

= 0,9828

0

50

100

150200

250

300

0 0,5 1 1,5 2

concentration [ppb]

absoptio

n

=228.8 nm

E t AA S

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Sample 1 (samp1) - ABO Pharma A-Z ;

Manufacturer: ABO Pharma AG

Sample 2 (samp2) - Supradyn ;

Manufacturer: Bayer AG

Sample 3 (samp3) - Supravit Active ;

Manufacturer: Kendy Ltd Bulgaria

Patented by Swisse AG

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IR spectra of benzocaine

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CH3, CH2

def. vibr.

CH3 (end)def. vibr.

C-H str. vibr

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KBr Spectra benzocaine

-NH2

-CH2

-C=O-NH2

-Ar

C-C

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Thank You for Your

attentionLora Georgieva

Denitsa AtanasovaKrum Bardarov